{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T14:52:21Z","timestamp":1787064741415,"version":"build-2736575974"},"reference-count":34,"publisher":"MDPI AG","issue":"18","license":[{"start":{"date-parts":[[2022,9,15]],"date-time":"2022-09-15T00:00:00Z","timestamp":1663200000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Federal University of Ouro Preto (UFOP)","award":["18\/2022"],"award-info":[{"award-number":["18\/2022"]}]},{"name":"FAPEMIG","award":["18\/2022"],"award-info":[{"award-number":["18\/2022"]}]},{"name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico (CNPq)","award":["18\/2022"],"award-info":[{"award-number":["18\/2022"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Modified release systems depend on the selection of an appropriate agent capable of controlling the release of the drug, sustaining the therapeutic action over time, and\/or releasing the drug at the level of a particular tissue or target organ. Polyethylene glycol 4000 (PEG 4000) is commonly employed in drug release formulations while polymethyl methacrylate (PMMA) is non-toxic and has a good solubility in organic solvents. This study aimed at the incorporation of ketoconazole in PMMA-g-PEG 4000 and its derivatives, thus evaluating its release profile and anti-Candida albicans and cytotoxic activities. Ketoconazole was characterized and incorporated into the copolymers. The ketoconazole incorporated in the copolymer and its derivatives showed an immediate release profile. All copolymers with ketoconazole showed activity against Candida albicans and were non-toxic to human cells in the entire concentration tested.<\/jats:p>","DOI":"10.3390\/ijms231810775","type":"journal-article","created":{"date-parts":[[2022,9,15]],"date-time":"2022-09-15T22:11:10Z","timestamp":1663279870000},"page":"10775","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Evaluation of Anti-Candida albicans Activity and Release of Ketoconazole in PMMA-G-PEG 4000 Films"],"prefix":"10.3390","volume":"23","author":[{"given":"Juliana Ribeiro","family":"Reynaldo","sequence":"first","affiliation":[{"name":"Department of Chemistry, Federal University of Ouro Preto (UFOP), Ouro Preto 35400-000, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0644-9197","authenticated-orcid":false,"given":"K\u00e1tia Monteiro","family":"Novack","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Federal University of Ouro Preto (UFOP), Ouro Preto 35400-000, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucas Resende Dutra","family":"Sousa","sequence":"additional","affiliation":[{"name":"Laboratory of Morphopathology, Center for Research in Biological Sciences, Federal University of Ouro Preto (UFOP), Morro do Cruzeiro Campus, Ouro Preto 35400-000, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7033-7686","authenticated-orcid":false,"given":"Paula Melo de Abreu","family":"Vieira","sequence":"additional","affiliation":[{"name":"Laboratory of Morphopathology, Center for Research in Biological Sciences, Federal University of Ouro Preto (UFOP), Morro do Cruzeiro Campus, Ouro Preto 35400-000, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6426-7113","authenticated-orcid":false,"given":"Tatiane Roquete","family":"Amparo","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Pharmaceutical Science, Federal University of Ouro Preto (UFOP), Morro do Cruzeiro Campus, Ouro Preto 35400-000, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1548-3274","authenticated-orcid":false,"given":"Gustavo Henrique Bianco de","family":"Souza","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Pharmaceutical Science, Federal University of Ouro Preto (UFOP), Morro do Cruzeiro Campus, Ouro Preto 35400-000, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luiz Fernando Medeiros","family":"Teixeira","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Pharmaceutical Science, Federal University of Ouro Preto (UFOP), Morro do Cruzeiro Campus, Ouro Preto 35400-000, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana Paula Moreira","family":"Barboza","sequence":"additional","affiliation":[{"name":"Physics Department, Federal University of Ouro Preto (UFOP), Morro do Cruzeiro Campus, Ouro Preto 35400-000, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0464-4754","authenticated-orcid":false,"given":"Bernardo Ruegger Almeida","family":"Neves","sequence":"additional","affiliation":[{"name":"Department of Physics, Federal University of Minas Gerais (UFMG), Belo Horizonte 31270-901, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meiry Edivirges","family":"Alvarenga","sequence":"additional","affiliation":[{"name":"Institute of Chemistry, Federal University of Goi\u00e1s (UFG), Samambaia Campus, Goi\u00e2nia 74690-900, GO, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Felipe Terra","family":"Martins","sequence":"additional","affiliation":[{"name":"Institute of Chemistry, Federal University of Goi\u00e1s (UFG), Samambaia Campus, Goi\u00e2nia 74690-900, GO, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0144-9518","authenticated-orcid":false,"given":"Viviane Martins Rebello dos","family":"Santos","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Federal University of Ouro Preto (UFOP), Ouro Preto 35400-000, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,9,15]]},"reference":[{"key":"ref_1","first-page":"21","article-title":"Polymers for Colon Targeted Drug Delivery: A Review","volume":"5","author":"Sharma","year":"2013","journal-title":"J. Drug Dev. Res."},{"key":"ref_2","unstructured":"Wilson, C., Mukherji, G., and Sha, H.K. (2008). Biopolymers and Colonic Delivery, Informa Healthcare."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"13","DOI":"10.4103\/0975-1483.51869","article-title":"Colon targeted drug delivery: Different approaches","volume":"1","author":"Jose","year":"2009","journal-title":"J. Young Pharm."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1175","DOI":"10.1211\/0022357011776612","article-title":"New strategies for polymer development in pharmaceutical science\u2014A short review","volume":"53","author":"Godwin","year":"2001","journal-title":"J. Pharm. Pharmacol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"97","DOI":"10.2174\/1567201052772906","article-title":"Mathematical Modelling and Controlled Drug Delivery: Matrix Systems","volume":"2","author":"Grassi","year":"2005","journal-title":"Curr. Drug Deliv."},{"key":"ref_6","unstructured":"Langer, R.S., and Wise, D.L. (1984). Medical Applications of Controlled Release, Applications and Evaluation, CRC Press. Volumes I\u2013II."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1527","DOI":"10.1126\/science.2218494","article-title":"New Methods of Drug Delivery","volume":"249","author":"Langer","year":"1990","journal-title":"Science"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1016\/S1367-5931(00)00226-X","article-title":"Drug delivery: An odyssey of 100 years","volume":"5","author":"Pillai","year":"2001","journal-title":"Curr. Opin. Chem. Biol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/S0378-5173(01)00804-3","article-title":"Controlled release of antifungal drug terbinafine hydrochloride from poly(N-vinyl 2-pyrrolidone\/itaconic acid) hydrogels","volume":"228","author":"Yakar","year":"2001","journal-title":"Int. J. Pharm."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"3181","DOI":"10.1021\/cr940351u","article-title":"Polymeric systems for controlled drug release","volume":"99","author":"Kathryn","year":"1999","journal-title":"Chem. Rev."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1590\/S0104-14282010005000009","article-title":"Aplica\u00e7\u00f5es Farmac\u00eauticas de Pol\u00edmeros","volume":"20","author":"Villanova","year":"2010","journal-title":"Pol\u00edmeros"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"447","DOI":"10.1016\/S1367-5931(00)00227-1","article-title":"Polymers in drug delivery","volume":"5","author":"Pillai","year":"2001","journal-title":"Curr. Opin. Chem. Biol."},{"key":"ref_13","first-page":"3","article-title":"A label-free Geno sensor for BRCA1 related sequence based on impedance spectroscopy","volume":"42","author":"Shuang","year":"2009","journal-title":"Macromolecules"},{"key":"ref_14","first-page":"29","article-title":"Pol\u00edmeros na Obten\u00e7\u00e3o de Sistemas de Libera\u00e7\u00e3o de f\u00e1rmacos","volume":"3","author":"Oliveira","year":"2006","journal-title":"Rev. Eletr\u00f4n. Farm."},{"key":"ref_15","first-page":"42","article-title":"Polymers for Controlled Release: Formulation Follows Function","volume":"29","author":"Rios","year":"2005","journal-title":"Pharm. Technol."},{"key":"ref_16","unstructured":"Mano, E.B., and Mendes, L.C. (1999). Introdu\u00e7\u00e3o a Pol\u00edmeros, Edgard Bl\u00fccher."},{"key":"ref_17","first-page":"167","article-title":"Preparation and release characteristics of Itraconazole polymeric films for topical application","volume":"5","author":"Mohamed","year":"2013","journal-title":"Int. J. Pharm. Pharm. Sci."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"028","DOI":"10.7324\/JAPS.2015.50505","article-title":"Formulation and Evaluation of Ketoconazole Polymeric Films for Topical Application","volume":"5","author":"Salama","year":"2015","journal-title":"J. Appl. Pharm. Sci."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1002\/masy.201300197","article-title":"Chemistry Modification of PMMA-g-PEG copolymer","volume":"343","author":"Silveira","year":"2014","journal-title":"Macromol. Symp."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1590\/0104-1428.1636","article-title":"S\u00edntese e Caracteriza\u00e7\u00e3o de novos copol\u00edmeros fosforilados","volume":"25","author":"NovacK","year":"2015","journal-title":"Pol\u00edmeros"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1700066","DOI":"10.1002\/masy.201700066","article-title":"Preparation, Characterization, and Biological Activity against Artemia Salina of News Copolymer PMMA-g-PEG Derivatives Incorporated with Fluconazole","volume":"378","author":"Novack","year":"2018","journal-title":"Macromol. Symp."},{"key":"ref_22","first-page":"685","article-title":"Novel Peg 4000 Derivatives and Its use in Controlled Release of Drug Indomethacin","volume":"43","author":"Nascimento","year":"2020","journal-title":"Qu\u00edm. Nova"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1800145","DOI":"10.1002\/masy.201800145","article-title":"Study of Controlled Release of PMMA-g-PEG Copolymer and Derivatives Incorporated with the Indomethacin Drug","volume":"381","author":"Azevedo","year":"2018","journal-title":"Macromol. Symp."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"111548","DOI":"10.1016\/j.msec.2020.111548","article-title":"QbD-driven formulation development and evaluation of topical hydrogel containing ketoconazole loaded cubosomes","volume":"119","author":"Rapalli","year":"2021","journal-title":"Mater. Sci. Eng. C"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"119","DOI":"10.4161\/viru.22913","article-title":"Candida albicans pathogenicity mechanisms","volume":"4","author":"Mayer","year":"2013","journal-title":"Virulence"},{"key":"ref_26","first-page":"5930489","article-title":"High Virulence and Antifungal Resistance in Clinical Strains of Candida albicans","volume":"2016","author":"Vaca","year":"2016","journal-title":"Can. J. Infect. Dis. Med. Microbiol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"3714329","DOI":"10.1155\/2018\/3714329","article-title":"Novel Intravaginal Drug Delivery System Based on Molecularly PEGylated Lipid Matrices for Improved Antifungal Activity of Miconazole Nitrate","volume":"2018","author":"Kenechukwu","year":"2018","journal-title":"BioMed Res. Int."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"4921","DOI":"10.1038\/s41598-019-41381-0","article-title":"Carbon nanotube incorporation in PMMA to prevent microbial adhesion","volume":"9","author":"Kim","year":"2019","journal-title":"Sci. Rep."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"8193","DOI":"10.1021\/la010909a","article-title":"Spread Coating of OPA on Mica:\u2009 From Multilayers to Self-Assembled Monolayers","volume":"17","author":"Neves","year":"2001","journal-title":"Langmuir"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1465","DOI":"10.1007\/s00396-015-3538-3","article-title":"Catharanthus roseus leaf extract-synthesized chitosan nanoparticles for controlled in vitro release of chloramphenicol and ketoconazole","volume":"293","author":"Nagaonkar","year":"2015","journal-title":"Colloid Polym. Sci."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"388","DOI":"10.1002\/hep.26208","article-title":"High lipophilicity and high daily dose of oral medications are associated with significant risk for drug-induced liver injury","volume":"58","author":"Chen","year":"2013","journal-title":"Hepatology"},{"key":"ref_32","unstructured":"Dick, M.K., Miao, J.H., and Limaiem, F. (2022). Histology, Fibroblast. StatPearls, StatPearls Publishing."},{"key":"ref_33","unstructured":"CLSI (2012). Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically, Clinical and Laboratory Standards Institute. [2nd ed.]."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1107","DOI":"10.1093\/jnci\/82.13.1107","article-title":"New Colorimetric Cytotoxicity Assay for Anticancer-Drug Screening","volume":"82","author":"Skehan","year":"1990","journal-title":"J. Natl. Cancer Inst."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/23\/18\/10775\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T00:32:22Z","timestamp":1760142742000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/23\/18\/10775"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,9,15]]},"references-count":34,"journal-issue":{"issue":"18","published-online":{"date-parts":[[2022,9]]}},"alternative-id":["ijms231810775"],"URL":"https:\/\/doi.org\/10.3390\/ijms231810775","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,9,15]]}}}