{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T04:32:36Z","timestamp":1789619556925,"version":"build-2803163510"},"reference-count":58,"publisher":"MDPI AG","issue":"18","license":[{"start":{"date-parts":[[2022,9,19]],"date-time":"2022-09-19T00:00:00Z","timestamp":1663545600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Scabies and hair lice are parasitic diseases that affect human skin and hair, respectively. The incidence and resistances of these infections are increasing. Tenutex\u00ae (disulfiram and benzyl benzoate emulsion) is an alternative to standard insecticides to avoid resistances. The aim of the work is to evaluate the transdermal absorption and the in vitro efficacy against scabies and hair lice after different exposition times. Dermatomed human skin was used to assess the dermal absorption using a validated High Performance Liquid Chromatography (HPLC) method. HEK001 keratinocytes were used to evaluate the cytotoxicity of benzyl benzoate. Only benzyl benzoate was able to cross the skin, but it did not show cytotoxicity at any of the tested concentrations. The product efficacy was tested on Psoroptes ovis after direct contact and after administration on sheep skin explants at different contact times. Permethrin\/malathion-resistant strains of Pediculus humanis capitis adults and eggs were directly exposed to Tenutex, and the vitality and hatchability, respectively, were evaluated. The anti-scabies study demonstrated that exposure for 6 or 24 h completely eradicated the parasite. The pediculicidal activity of Tenutex exhibited superior efficacy than standard treatment on resistant lice. The positive results obtained suggest that Tenutex\u00ae is a good treatment option, especially in drug resistance situations.<\/jats:p>","DOI":"10.3390\/ijms231810969","type":"journal-article","created":{"date-parts":[[2022,9,19]],"date-time":"2022-09-19T21:47:27Z","timestamp":1663624047000},"page":"10969","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["The Efficacy and Biopharmaceutical Properties of a Fixed-Dose Combination of Disulfiram and Benzyl Benzoate"],"prefix":"10.3390","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4080-3516","authenticated-orcid":false,"given":"Maria","family":"Lajarin-Reinares","sequence":"first","affiliation":[{"name":"Department of Genetics and Microbiology, Campus Microbiology Unit, Autonomous Barcelona University, 08193 Barcelona, Spain"},{"name":"Topical & Oral Development, Research and Development Reig Jofre Laboratories, 08970 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elia","family":"Martinez-Esteve","sequence":"additional","affiliation":[{"name":"Topical & Oral Development, Research and Development Reig Jofre Laboratories, 08970 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8743-7229","authenticated-orcid":false,"given":"Eloy","family":"Pena-Rodr\u00edguez","sequence":"additional","affiliation":[{"name":"Topical & Oral Development, Research and Development Reig Jofre Laboratories, 08970 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2383-7300","authenticated-orcid":false,"given":"Mariona","family":"Ca\u00f1ellas-Santos","sequence":"additional","affiliation":[{"name":"Biotechnology Department, Research and Development, Reig Jofre Laboratories, 08970 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sanja","family":"Bulut","sequence":"additional","affiliation":[{"name":"Department of Development, Bioglan AB, 21124 Malm\u00f6, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5152-2579","authenticated-orcid":false,"given":"Kostas","family":"Karabelas","sequence":"additional","affiliation":[{"name":"Department of Development, Bioglan AB, 21124 Malm\u00f6, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6381-324X","authenticated-orcid":false,"given":"Adam","family":"Clauss","sequence":"additional","affiliation":[{"name":"Department of Development, Bioglan AB, 21124 Malm\u00f6, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carles","family":"Nieto","sequence":"additional","affiliation":[{"name":"Topical & Oral Development, Research and Development Reig Jofre Laboratories, 08970 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9316-8459","authenticated-orcid":false,"given":"Mireia","family":"Mallandrich","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Pharmaceutical Technology and Physical Chemistry, School of Pharmacy and Food Sciences, University of Barcelona, 08028 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8224-2932","authenticated-orcid":false,"given":"Francisco","family":"Fernandez-Campos","sequence":"additional","affiliation":[{"name":"Topical & Oral Development, Research and Development Reig Jofre Laboratories, 08970 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,9,19]]},"reference":[{"key":"ref_1","first-page":"757","article-title":"The Treatment of Scabies: A Systematic Review of Randomized Controlled Trials","volume":"113","author":"Dressler","year":"2016","journal-title":"Dtsch. 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