{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T23:32:13Z","timestamp":1790811133106,"version":"4.1.0"},"reference-count":80,"publisher":"MDPI AG","issue":"19","license":[{"start":{"date-parts":[[2022,9,20]],"date-time":"2022-09-20T00:00:00Z","timestamp":1663632000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Natural Science Foundation of China","award":["41977120"],"award-info":[{"award-number":["41977120"]}]},{"name":"National Natural Science Foundation of China","award":["42177031"],"award-info":[{"award-number":["42177031"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Cigarette smoke exposure has a harmful impact on health and increases the risk of disease. However, studies on cigarette-smoke-induced adverse effects from the perspective of the gut\u2013liver axis are lacking. In this study, we evaluated the adverse effects of cigarette smoke exposure on mice through physiological, biochemical, and histopathological analyses and explored cigarette-smoke-induced gut microbiota imbalance and changes in liver gene expression through a multiomics analysis. We demonstrated that cigarette smoke exposure caused abnormal physiological indices (including reduced body weight, blood lipids, and food intake) in mice, which also triggered liver injury and induced disorders of the gut microbiota and liver transcriptome (especially lipid metabolism). A significant correlation between intestinal bacterial abundance and the expression of lipid-metabolism-related genes was detected, suggesting the coordinated regulation of lipid metabolism by gut microbiota and liver metabolism. Specifically, Salmonella (harmful bacterium) was negatively and positively correlated with up- (such as Acsl3 and Me1) and downregulated genes (such as Angptl4, Cyp4a12a, and Plin5) involved in lipid metabolism, while Ligilactobacillus (beneficial bacterium) showed opposite trends with these genes. Our results clarified the key role of gut microbiota in liver damage and metabolism and improved the understanding of gut\u2013liver interactions caused by cigarette smoke exposure.<\/jats:p>","DOI":"10.3390\/ijms231911008","type":"journal-article","created":{"date-parts":[[2022,9,20]],"date-time":"2022-09-20T22:36:44Z","timestamp":1663713404000},"page":"11008","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["Effects of Cigarette Smoke Exposure on the Gut Microbiota and Liver Transcriptome in Mice Reveal Gut\u2013Liver Interactions"],"prefix":"10.3390","volume":"23","author":[{"given":"Lei","family":"Meng","sequence":"first","affiliation":[{"name":"Key Laboratory of Agricultural and Environmental Microbiology, Department of Microbiology, College of Life Sciences, Nanjing Agricultural University, Ministry of Agriculture and Rural Affairs, Nanjing 210095, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mengjun","family":"Xu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Agricultural and Environmental Microbiology, Department of Microbiology, College of Life Sciences, Nanjing Agricultural University, Ministry of Agriculture and Rural Affairs, Nanjing 210095, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Youwen","family":"Xing","sequence":"additional","affiliation":[{"name":"Key Laboratory of Agricultural and Environmental Microbiology, Department of Microbiology, College of Life Sciences, Nanjing Agricultural University, Ministry of Agriculture and Rural Affairs, Nanjing 210095, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3936-523X","authenticated-orcid":false,"given":"Chen","family":"Chen","sequence":"additional","affiliation":[{"name":"Key Laboratory of Agricultural and Environmental Microbiology, Department of Microbiology, College of Life Sciences, Nanjing Agricultural University, Ministry of Agriculture and Rural Affairs, Nanjing 210095, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiandong","family":"Jiang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Agricultural and Environmental Microbiology, Department of Microbiology, College of Life Sciences, Nanjing Agricultural University, Ministry of Agriculture and Rural Affairs, Nanjing 210095, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2092-9987","authenticated-orcid":false,"given":"Xihui","family":"Xu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Agricultural and Environmental Microbiology, Department of Microbiology, College of Life Sciences, Nanjing Agricultural University, Ministry of Agriculture and Rural Affairs, Nanjing 210095, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,9,20]]},"reference":[{"key":"ref_1","unstructured":"World Health Organization (2021). 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