{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,30]],"date-time":"2026-05-30T01:14:40Z","timestamp":1780103680384,"version":"3.54.0"},"reference-count":39,"publisher":"MDPI AG","issue":"19","license":[{"start":{"date-parts":[[2022,9,22]],"date-time":"2022-09-22T00:00:00Z","timestamp":1663804800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Sichuan Agricultural University","award":["2221993246"],"award-info":[{"award-number":["2221993246"]}]},{"name":"Sichuan Agricultural University","award":["2022NSFSC0070"],"award-info":[{"award-number":["2022NSFSC0070"]}]},{"name":"Sichuan Agricultural University","award":["2021YFYZ0015"],"award-info":[{"award-number":["2021YFYZ0015"]}]},{"name":"the Natural Science Foundation of Sichuan Province of China","award":["2221993246"],"award-info":[{"award-number":["2221993246"]}]},{"name":"the Natural Science Foundation of Sichuan Province of China","award":["2022NSFSC0070"],"award-info":[{"award-number":["2022NSFSC0070"]}]},{"name":"the Natural Science Foundation of Sichuan Province of China","award":["2021YFYZ0015"],"award-info":[{"award-number":["2021YFYZ0015"]}]},{"name":"the Project of Provincial 14th Five Years\u2019 Animal Breeding of Sichuan Province","award":["2221993246"],"award-info":[{"award-number":["2221993246"]}]},{"name":"the Project of Provincial 14th Five Years\u2019 Animal Breeding of Sichuan Province","award":["2022NSFSC0070"],"award-info":[{"award-number":["2022NSFSC0070"]}]},{"name":"the Project of Provincial 14th Five Years\u2019 Animal Breeding of Sichuan Province","award":["2021YFYZ0015"],"award-info":[{"award-number":["2021YFYZ0015"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>A. hydrophila is an important pathogen that mainly harms aquatic animals and has exhibited resistance to a variety of antibiotics. Here, to seek an effective alternative for antibiotics, the effects of umbelliferone (UM) at sub-MICs on A. hydrophila virulence factors and the quorum-sensing system were studied. Subsequently, RNA sequencing was employed to explore the potential mechanisms for the antivirulence activity of umbelliferone. Meanwhile, the protective effect of umbelliferone on grass carp infected with A. hydrophila was studied in vivo. Our results indicated that umbelliferone could significantly inhibit A. hydrophila virulence such as hemolysis, biofilm formation, swimming and swarming motility, and their quorum-sensing signals AHL and AI-2. Transcriptomic analysis showed that umbelliferone downregulated expression levels of genes related to exotoxin, the secretory system (T2SS and T6SS), iron uptake, etc. Animal studies demonstrated that umbelliferone could significantly improve the survival of grass carps infected with A. hydrophila, reduce the bacterial load in the various tissues, and ameliorate cardiac, splenic, and hepatopancreas injury. Collectively, umbelliferone can reduce the pathogenicity of A. hydrophila and is a potential drug for treating A. hydrophila infection.<\/jats:p>","DOI":"10.3390\/ijms231911119","type":"journal-article","created":{"date-parts":[[2022,9,22]],"date-time":"2022-09-22T05:33:14Z","timestamp":1663824794000},"page":"11119","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["The Antivirulence Activity of Umbelliferone and Its Protective Effect against A. hydrophila-Infected Grass Carp"],"prefix":"10.3390","volume":"23","author":[{"given":"Ling","family":"Zhao","sequence":"first","affiliation":[{"name":"College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoyu","family":"Jin","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ziqian","family":"Xiong","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7944-029X","authenticated-orcid":false,"given":"Huaqiao","family":"Tang","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongrui","family":"Guo","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gang","family":"Ye","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Defang","family":"Chen","sequence":"additional","affiliation":[{"name":"Institute of Animal Genetics and Breeding, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shiyong","family":"Yang","sequence":"additional","affiliation":[{"name":"Institute of Animal Genetics and Breeding, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhongqiong","family":"Yin","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hualin","family":"Fu","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 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