{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T19:44:22Z","timestamp":1790192662274,"version":"4.1.0"},"reference-count":44,"publisher":"MDPI AG","issue":"19","license":[{"start":{"date-parts":[[2022,9,23]],"date-time":"2022-09-23T00:00:00Z","timestamp":1663891200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Ministry of Education, Culture, Sports, Science and Technology (MEXT)","award":["15\u2009K10881"],"award-info":[{"award-number":["15\u2009K10881"]}]},{"name":"Ministry of Education, Culture, Sports, Science and Technology (MEXT)","award":["18\u2009K09424"],"award-info":[{"award-number":["18\u2009K09424"]}]},{"name":"Ministry of Education, Culture, Sports, Science and Technology (MEXT)","award":["JPMJSP2123"],"award-info":[{"award-number":["JPMJSP2123"]}]},{"name":"JST SPRING","award":["15\u2009K10881"],"award-info":[{"award-number":["15\u2009K10881"]}]},{"name":"JST SPRING","award":["18\u2009K09424"],"award-info":[{"award-number":["18\u2009K09424"]}]},{"name":"JST SPRING","award":["JPMJSP2123"],"award-info":[{"award-number":["JPMJSP2123"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Retinal ischemia\/reperfusion (I\/R) injury can cause severe vision impairment. Retinal I\/R injury is associated with pathological increases in reactive oxygen species and inflammation, resulting in retinal neuronal cell death. To date, effective therapies have not been developed. Nicotinamide mononucleotide (NMN), a key nicotinamide adenine dinucleotide (NAD+) intermediate, has been shown to exert neuroprotection for retinal diseases. However, it remains unclear whether NMN can prevent retinal I\/R injury. Thus, we aimed to determine whether NMN therapy is useful for retinal I\/R injury-induced retinal degeneration. One day after NMN intraperitoneal (IP) injection, adult mice were subjected to retinal I\/R injury. Then, the mice were injected with NMN once every day for three days. Electroretinography and immunohistochemistry were used to measure retinal functional alterations and retinal inflammation, respectively. The protective effect of NMN administration was further examined using a retinal cell line, 661W, under CoCl2-induced oxidative stress conditions. NMN IP injection significantly suppressed retinal functional damage, as well as inflammation. NMN treatment showed protective effects against oxidative stress-induced cell death. The antioxidant pathway (Nrf2 and Hmox-1) was activated by NMN treatment. In conclusion, NMN could be a promising preventive neuroprotective drug for ischemic retinopathy.<\/jats:p>","DOI":"10.3390\/ijms231911228","type":"journal-article","created":{"date-parts":[[2022,9,25]],"date-time":"2022-09-25T23:13:27Z","timestamp":1664147607000},"page":"11228","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Nicotinamide Mononucleotide Prevents Retinal Dysfunction in a Mouse Model of Retinal Ischemia\/Reperfusion Injury"],"prefix":"10.3390","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5992-9039","authenticated-orcid":false,"given":"Deokho","family":"Lee","sequence":"first","affiliation":[{"name":"Laboratory of Photobiology, Keio University School of Medicine, Shinjuku-ku, Tokyo 160-8582, Japan"},{"name":"Department of Ophthalmology, Keio University School of Medicine, Shinjuku-ku, Tokyo 160-8582, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1013-5737","authenticated-orcid":false,"given":"Yohei","family":"Tomita","sequence":"additional","affiliation":[{"name":"Laboratory of Photobiology, Keio University School of Medicine, Shinjuku-ku, Tokyo 160-8582, Japan"},{"name":"Department of Ophthalmology, Keio University School of Medicine, Shinjuku-ku, Tokyo 160-8582, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5377-6772","authenticated-orcid":false,"given":"Yukihiro","family":"Miwa","sequence":"additional","affiliation":[{"name":"Laboratory of Photobiology, Keio University School of Medicine, Shinjuku-ku, Tokyo 160-8582, Japan"},{"name":"Department of Ophthalmology, Keio University School of Medicine, Shinjuku-ku, Tokyo 160-8582, Japan"},{"name":"Aichi Animal Eye Clinic, Nagoya 466-0827, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2322-0332","authenticated-orcid":false,"given":"Ari","family":"Shinojima","sequence":"additional","affiliation":[{"name":"Laboratory of Photobiology, Keio University School of Medicine, Shinjuku-ku, Tokyo 160-8582, Japan"},{"name":"Department of Ophthalmology, Keio University School of Medicine, Shinjuku-ku, Tokyo 160-8582, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Norimitsu","family":"Ban","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Keio University School of Medicine, Shinjuku-ku, Tokyo 160-8582, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4917-033X","authenticated-orcid":false,"given":"Shintaro","family":"Yamaguchi","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Keio University School of Medicine, Shinjuku-ku, Tokyo 160-8582, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ken","family":"Nishioka","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Keio University School of Medicine, Shinjuku-ku, Tokyo 160-8582, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2892-9810","authenticated-orcid":false,"given":"Kazuno","family":"Negishi","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Keio University School of Medicine, Shinjuku-ku, Tokyo 160-8582, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Yoshino","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Keio University School of Medicine, Shinjuku-ku, Tokyo 160-8582, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5457-2720","authenticated-orcid":false,"given":"Toshihide","family":"Kurihara","sequence":"additional","affiliation":[{"name":"Laboratory of Photobiology, Keio University School of Medicine, Shinjuku-ku, Tokyo 160-8582, Japan"},{"name":"Department of Ophthalmology, Keio University School of Medicine, Shinjuku-ku, Tokyo 160-8582, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,9,23]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"501","DOI":"10.1006\/exer.1996.0140","article-title":"Inducible nitric oxide synthase in retinal ischemia-reperfusion injury","volume":"63","author":"Hangai","year":"1996","journal-title":"Exp. 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