{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T01:23:08Z","timestamp":1783387388116,"version":"3.54.6"},"reference-count":71,"publisher":"MDPI AG","issue":"19","license":[{"start":{"date-parts":[[2022,9,26]],"date-time":"2022-09-26T00:00:00Z","timestamp":1664150400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"RSF","award":["#21-44-07002"],"award-info":[{"award-number":["#21-44-07002"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>The power of most of the enterobacterial O antigen types to provide robust protection against direct recognition of the cell surface by bacteriophage receptor-recognition proteins (RBP) has been recently recognized. The bacteriophages infecting O antigen producing strains of E. coli employ various strategies to tackle this nonspecific protection. T-even related phages, including RB49-like viruses, often have wide host ranges, being considered good candidates for use in phage therapy. However, the mechanisms by which these phages overcome the O antigen barrier remain unknown. We demonstrate here that RB49 and related phages Cognac49 and Whisky49 directly use certain types of O antigen as their primary receptors recognized by the virus long tail fibers (LTF) RBP gp38, so the O antigen becomes an attractant instead of an obstacle. Simultaneously to recognize multiple O antigen types, LTFs of each of these phages can bind to additional receptors, such as OmpA protein, enabling them to infect some rough strains of E. coli. We speculate that the mechanical force of the deployment of the short tail fibers (STF) triggered by the LTF binding to the O antigen or underneath of it, allows the receptor binding domains of STF to break through the O polysaccharide layer.<\/jats:p>","DOI":"10.3390\/ijms231911329","type":"journal-article","created":{"date-parts":[[2022,9,27]],"date-time":"2022-09-27T23:12:12Z","timestamp":1664320332000},"page":"11329","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["RB49-like Bacteriophages Recognize O Antigens as One of the Alternative Primary Receptors"],"prefix":"10.3390","volume":"23","author":[{"given":"Alexandr D.","family":"Efimov","sequence":"first","affiliation":[{"name":"Laboratory of Microbial Viruses, Winogradsky Institute of Microbiology RC Biotechnology RAS, 117312 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alla K.","family":"Golomidova","sequence":"additional","affiliation":[{"name":"Laboratory of Microbial Viruses, Winogradsky Institute of Microbiology RC Biotechnology RAS, 117312 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9101-1543","authenticated-orcid":false,"given":"Eugene E.","family":"Kulikov","sequence":"additional","affiliation":[{"name":"Laboratory of Microbial Viruses, Winogradsky Institute of Microbiology RC Biotechnology RAS, 117312 Moscow, Russia"},{"name":"Faculty of Biology, Lomonosov Moscow State University, 119991 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5447-4872","authenticated-orcid":false,"given":"Ilya S.","family":"Belalov","sequence":"additional","affiliation":[{"name":"Laboratory of Microbial Viruses, Winogradsky Institute of Microbiology RC Biotechnology RAS, 117312 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pavel A.","family":"Ivanov","sequence":"additional","affiliation":[{"name":"Laboratory of Microbial Viruses, Winogradsky Institute of Microbiology RC Biotechnology RAS, 117312 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrey V.","family":"Letarov","sequence":"additional","affiliation":[{"name":"Laboratory of Microbial Viruses, Winogradsky Institute of Microbiology RC Biotechnology RAS, 117312 Moscow, Russia"},{"name":"Faculty of Biology, Lomonosov Moscow State University, 119991 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,9,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Thanki, A.M., Taylor-Joyce, G., Dowah, A., Nale, J.Y., Malik, D., and Clokie, M.R.J. 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