{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T04:48:08Z","timestamp":1787028488145,"version":"build-2736575974"},"reference-count":52,"publisher":"MDPI AG","issue":"19","license":[{"start":{"date-parts":[[2022,10,1]],"date-time":"2022-10-01T00:00:00Z","timestamp":1664582400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Romanian Ministry of Research, Innovation and Digitalization","award":["PN-III-P2-2.1-PED-2019-2436-PED 396 project"],"award-info":[{"award-number":["PN-III-P2-2.1-PED-2019-2436-PED 396 project"]}]},{"name":"Romanian Ministry of Research, Innovation and Digitalization","award":["Finance Excellence (PFE)\u20148\/2021"],"award-info":[{"award-number":["Finance Excellence (PFE)\u20148\/2021"]}]},{"name":"Romanian Ministry of Research, Innovation and Digitalization","award":["PN-III-P2-2.1-PED-2019-2436-PED 396 project"],"award-info":[{"award-number":["PN-III-P2-2.1-PED-2019-2436-PED 396 project"]}]},{"name":"Romanian Ministry of Research, Innovation and Digitalization","award":["Finance Excellence (PFE)\u20148\/2021"],"award-info":[{"award-number":["Finance Excellence (PFE)\u20148\/2021"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>(1) The present study tested in vitro the capacity of a fermented rapeseed meal extract to reduce medicinal ZnO, which will be banned at the EU level from 2023 onwards because of its potential to cause environmental pollution and the development of Zn resistance in gut bacteria. Rapeseed meal could be an important ZnO substitute as it has antioxidant\/radical scavenging properties due to its content of bioactive compounds (e.g., polyphenols). (2) Protein array and flow cytometry were used to detect apoptosis, oxidative stress production, and inflammatory and signaling-related molecules in Caco-2 and goblet HT29-MTX co-culture cells challenged with Escherichia coli lipopolysaccharides and treated with ZnO and FRSM. (3) LPS induced cell death (21.1% vs. 12.7% in control, p &lt; 0.005); apoptosis (16.6%); ROS production; and overexpression of biomarkers related to inflammation (63.15% cytokines and 66.67% chemokines), oxidative stress, and signaling proteins when compared to untreated cells. ZnO was effective in counteracting the effect of LPS, and 73.68% cytokines and 91.67% of chemokines were recovered. FRSM was better at restoring normal protein expression for 78.94% of cytokines, 91.67% of chemokines, and 61.11% of signaling molecules. FRSM was able to mitigate negative effects of LPS and might be an alternative to ZnO in pig diets.<\/jats:p>","DOI":"10.3390\/ijms231911640","type":"journal-article","created":{"date-parts":[[2022,10,8]],"date-time":"2022-10-08T22:19:27Z","timestamp":1665267567000},"page":"11640","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Yeast-Fermented Rapeseed Meal Extract Is Able to Reduce Inflammation and Oxidative Stress Caused by Escherichia coli Lipopolysaccharides and to Replace ZnO in Caco-2\/HTX29 Co-Culture Cells"],"prefix":"10.3390","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0727-5827","authenticated-orcid":false,"given":"Ionelia","family":"Taranu","sequence":"first","affiliation":[{"name":"Laboratory of Animal Biology, National Institute for Research and Development for Biology and Animal Nutrition, Calea Bucuresti No. 1, Balotesti, 077015 Ilfov, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1139-604X","authenticated-orcid":false,"given":"Gina Cecilia","family":"Pistol","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Biology, National Institute for Research and Development for Biology and Animal Nutrition, Calea Bucuresti No. 1, Balotesti, 077015 Ilfov, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrei Cristian","family":"Anghel","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Biology, National Institute for Research and Development for Biology and Animal Nutrition, Calea Bucuresti No. 1, Balotesti, 077015 Ilfov, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniela","family":"Marin","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Biology, National Institute for Research and Development for Biology and Animal Nutrition, Calea Bucuresti No. 1, Balotesti, 077015 Ilfov, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3909-9373","authenticated-orcid":false,"given":"Cristina","family":"Bulgaru","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Biology, National Institute for Research and Development for Biology and Animal Nutrition, Calea Bucuresti No. 1, Balotesti, 077015 Ilfov, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,10,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"761742","DOI":"10.3389\/fvets.2021.761742","article-title":"Effect of Resin Acid and Zinc Oxide on Immune Status of Weaned Piglets Challenged With, E. coli Lipopolysaccharide","volume":"8","author":"Guan","year":"2021","journal-title":"Front. 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