{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T22:20:32Z","timestamp":1782858032874,"version":"3.54.5"},"reference-count":51,"publisher":"MDPI AG","issue":"19","license":[{"start":{"date-parts":[[2022,10,1]],"date-time":"2022-10-01T00:00:00Z","timestamp":1664582400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Diabetes UK Harry Keen Intermediate Clinical Fellowship","award":["DUK-HKF 17\/0005712"],"award-info":[{"award-number":["DUK-HKF 17\/0005712"]}]},{"name":"Diabetes UK Harry Keen Intermediate Clinical Fellowship","award":["NNF19SA058974"],"award-info":[{"award-number":["NNF19SA058974"]}]},{"name":"Diabetes UK Harry Keen Intermediate Clinical Fellowship","award":["BB\/M027252\/1"],"award-info":[{"award-number":["BB\/M027252\/1"]}]},{"name":"European Foundation for the Study of Diabetes\u2014Novo Nordisk Foundation Future Leaders\u2019 Award","award":["DUK-HKF 17\/0005712"],"award-info":[{"award-number":["DUK-HKF 17\/0005712"]}]},{"name":"European Foundation for the Study of Diabetes\u2014Novo Nordisk Foundation Future Leaders\u2019 Award","award":["NNF19SA058974"],"award-info":[{"award-number":["NNF19SA058974"]}]},{"name":"European Foundation for the Study of Diabetes\u2014Novo Nordisk Foundation Future Leaders\u2019 Award","award":["BB\/M027252\/1"],"award-info":[{"award-number":["BB\/M027252\/1"]}]},{"DOI":"10.13039\/501100000268","name":"BBSRC","doi-asserted-by":"publisher","award":["DUK-HKF 17\/0005712"],"award-info":[{"award-number":["DUK-HKF 17\/0005712"]}],"id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000268","name":"BBSRC","doi-asserted-by":"publisher","award":["NNF19SA058974"],"award-info":[{"award-number":["NNF19SA058974"]}],"id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000268","name":"BBSRC","doi-asserted-by":"publisher","award":["BB\/M027252\/1"],"award-info":[{"award-number":["BB\/M027252\/1"]}],"id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"publisher"}]},{"name":"NIHR Cambridge BRC","award":["DUK-HKF 17\/0005712"],"award-info":[{"award-number":["DUK-HKF 17\/0005712"]}]},{"name":"NIHR Cambridge BRC","award":["NNF19SA058974"],"award-info":[{"award-number":["NNF19SA058974"]}]},{"name":"NIHR Cambridge BRC","award":["BB\/M027252\/1"],"award-info":[{"award-number":["BB\/M027252\/1"]}]},{"name":"core biochemical assay laboratory (CBAL)","award":["DUK-HKF 17\/0005712"],"award-info":[{"award-number":["DUK-HKF 17\/0005712"]}]},{"name":"core biochemical assay laboratory (CBAL)","award":["NNF19SA058974"],"award-info":[{"award-number":["NNF19SA058974"]}]},{"name":"core biochemical assay laboratory (CBAL)","award":["BB\/M027252\/1"],"award-info":[{"award-number":["BB\/M027252\/1"]}]},{"name":"core metabolomic and lipidomic laboratory (CMAL)","award":["DUK-HKF 17\/0005712"],"award-info":[{"award-number":["DUK-HKF 17\/0005712"]}]},{"name":"core metabolomic and lipidomic laboratory (CMAL)","award":["NNF19SA058974"],"award-info":[{"award-number":["NNF19SA058974"]}]},{"name":"core metabolomic and lipidomic laboratory (CMAL)","award":["BB\/M027252\/1"],"award-info":[{"award-number":["BB\/M027252\/1"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Male fertility, as manifest by the quantity and progressive motility of spermatozoa, is negatively impacted by obesity, dyslipidaemia and metabolic disease. However, the relative distribution of lipids in spermatozoa and the two compartments which supply lipids for spermatogenesis (seminal fluid and blood serum) has not been studied. We hypothesised that altered availability of lipids in blood serum and seminal fluid may affect the lipid composition and progressive motility of sperm. 60 men of age 35 years (median (range 20\u201345) and BMI 30.4 kg\/m2 (24\u201336.5) under preliminary investigation for subfertility were recruited at an NHS clinic. Men provided samples of serum and semen, subject to strict acceptance criteria, for analysis of spermatozoa count and motility. Blood serum (n = 60), spermatozoa (n = 26) and seminal fluid (n = 60) were frozen for batch lipidomics analysis. Spermatozoa and seminal fluid had comparable lipid composition but showed marked differences with the serum lipidome. Spermatozoa demonstrated high abundance of ceramides, very-long-chain fatty acids (C20-22), and certain phospholipids (sphingomyelins, plasmalogens, phosphatidylethanolamines) with low abundance of phosphatidylcholines, cholesterol and triglycerides. Men with spermatozoa of low progressive motility had evidence of fewer concentration gradients for many lipid species between blood serum and spermatozoa compartments. Spermatozoa are abundant in multiple lipid species which are likely to contribute to key cellular functions. Lipid metabolism shows reduced regulation between compartments in men with spermatozoa with reduced progressive motility.<\/jats:p>","DOI":"10.3390\/ijms231911655","type":"journal-article","created":{"date-parts":[[2022,10,8]],"date-time":"2022-10-08T22:19:27Z","timestamp":1665267567000},"page":"11655","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Relative Abundance of Lipid Metabolites in Spermatozoa across Three Compartments"],"prefix":"10.3390","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4267-2051","authenticated-orcid":false,"given":"Samuel","family":"Furse","sequence":"first","affiliation":[{"name":"Wellcome Trust-MRC Institute of Metabolic Science, University of Cambridge, Box 289, Cambridge Biomedical Campus, Hills Road, Cambridge CB2 0QQ, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura C.","family":"Kusinski","sequence":"additional","affiliation":[{"name":"Wellcome Trust-MRC Institute of Metabolic Science, University of Cambridge, Box 289, Cambridge Biomedical Campus, Hills Road, Cambridge CB2 0QQ, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alison","family":"Ray","sequence":"additional","affiliation":[{"name":"Department of Clinical Chemistry and Immunology, Peterborough City Hospital, North West Anglia NHS Foundation Trust, Bretton Gate, Peterborough PE3 9GZ, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Coralie","family":"Glenn-Sansum","sequence":"additional","affiliation":[{"name":"R&D Department, Peterborough City Hospital, North West Anglia NHS Foundation Trust, Bretton Gate, Peterborough PE3 9GZ, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huw E. L.","family":"Williams","sequence":"additional","affiliation":[{"name":"Centre for Biomolecular Sciences, School of Chemistry, University of Nottingham, University Park, Nottingham NG7 2RD, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9998-051X","authenticated-orcid":false,"given":"Albert","family":"Koulman","sequence":"additional","affiliation":[{"name":"Wellcome Trust-MRC Institute of Metabolic Science, University of Cambridge, Box 289, Cambridge Biomedical Campus, Hills Road, Cambridge CB2 0QQ, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claire L.","family":"Meek","sequence":"additional","affiliation":[{"name":"Wellcome Trust-MRC Institute of Metabolic Science, University of Cambridge, Box 289, Cambridge Biomedical Campus, Hills Road, Cambridge CB2 0QQ, UK"},{"name":"Department of Clinical Chemistry and Immunology, Peterborough City Hospital, North West Anglia NHS Foundation Trust, Bretton Gate, Peterborough PE3 9GZ, UK"},{"name":"Department of Clinical Biochemistry, Cambridge Universities NHS Foundation Trust, Hills Road, Cambridge CB2 0QQ, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,10,1]]},"reference":[{"key":"ref_1","first-page":"e184","article-title":"Fertility and the aging male","volume":"13","author":"Harris","year":"2011","journal-title":"Rev. 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