{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T07:10:27Z","timestamp":1791443427924,"version":"4.3.3"},"reference-count":48,"publisher":"MDPI AG","issue":"19","license":[{"start":{"date-parts":[[2022,10,3]],"date-time":"2022-10-03T00:00:00Z","timestamp":1664755200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005795","name":"Chang Gung Memorial Hospital, Linkou, Taiwan","doi-asserted-by":"publisher","award":["CMRPG 11002"],"award-info":[{"award-number":["CMRPG 11002"]}],"id":[{"id":"10.13039\/501100005795","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Staphylococcus aureus is an important pathogen for keratitis, a vision-threatening disease. We aimed to investigate the genotyping, antibiotic susceptibility, and clinical features of S. aureus keratitis, and to explore the possible role of Panton\u2013Valentine leucocidin (PVL), a major virulence factor of S. aureus. We recruited 49 patients with culture-proven S. aureus keratitis between 2013 and 2017 at Chang Gung Memorial Hospital, Taiwan. PVL gene, multilocus sequence type (MLST), staphylococcal cassette chromosome mec (SCCmec), and pulsed-field gel electrophoresis (PFGE) were performed. Antibiotic susceptibility was verified using disk diffusion\/E test. There were 49 patients with S. aureus keratitis; 17 (34.7%) were caused by methicillin-resistant S. aureus (MRSA) and 9 (18.4%) isolates had PVL genes. The predominant genotyping of MRSA isolates was CC59\/PFGE type D\/SCCmec VT\/PVL (+). All methicillin-sensitive S. aureus (MSSA) and approximately 60% MRSA were susceptible to fluoroquinolones. No significant differences in clinical features, treatments, and visual outcomes were observed between MRSA\/MSSA or PVL(+)\/PVL(\u2212) groups. In Taiwan, approximately one third of S. aureus keratitis was caused by MRSA, mainly community-associated MRSA. Although MRSA isolates were more resistant than MSSA, clinical characteristics were similar between two groups. Fluoroquinolones could be good empiric antibiotics for S. aureus keratitis.<\/jats:p>","DOI":"10.3390\/ijms231911703","type":"journal-article","created":{"date-parts":[[2022,10,8]],"date-time":"2022-10-08T23:39:31Z","timestamp":1665272371000},"page":"11703","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Staphylococcus aureus Keratitis in Taiwan: Genotyping, Antibiotic Susceptibility, and Clinical Features"],"prefix":"10.3390","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5745-9371","authenticated-orcid":false,"given":"Ching-Hsi","family":"Hsiao","sequence":"first","affiliation":[{"name":"Department of Ophthalmology, Chang Gung Memorial Hospital, Linkou Medical Center, No. 5 Fu-Hsin Rd., Kweishan, Taoyuan 333, Taiwan"},{"name":"College of Medicine, Chang Gung University, No. 261, Wenhua 1st Rd., Kweishan, Taoyuan 333, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6814-6530","authenticated-orcid":false,"given":"Eugene Yu-Chuan","family":"Kang","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Chang Gung Memorial Hospital, Linkou Medical Center, No. 5 Fu-Hsin Rd., Kweishan, Taoyuan 333, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lung-Kun","family":"Yeh","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Chang Gung Memorial Hospital, Linkou Medical Center, No. 5 Fu-Hsin Rd., Kweishan, Taoyuan 333, Taiwan"},{"name":"College of Medicine, Chang Gung University, No. 261, Wenhua 1st Rd., Kweishan, Taoyuan 333, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David H. K.","family":"Ma","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Chang Gung Memorial Hospital, Linkou Medical Center, No. 5 Fu-Hsin Rd., Kweishan, Taoyuan 333, Taiwan"},{"name":"College of Medicine, Chang Gung University, No. 261, Wenhua 1st Rd., Kweishan, Taoyuan 333, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1117-7878","authenticated-orcid":false,"given":"Hung-Chi","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Chang Gung Memorial Hospital, Linkou Medical Center, No. 5 Fu-Hsin Rd., Kweishan, Taoyuan 333, Taiwan"},{"name":"College of Medicine, Chang Gung University, No. 261, Wenhua 1st Rd., Kweishan, Taoyuan 333, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6298-5269","authenticated-orcid":false,"given":"Kuo-Hsuan","family":"Hung","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Chang Gung Memorial Hospital, Linkou Medical Center, No. 5 Fu-Hsin Rd., Kweishan, Taoyuan 333, Taiwan"},{"name":"College of Medicine, Chang Gung University, No. 261, Wenhua 1st Rd., Kweishan, Taoyuan 333, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4351-8953","authenticated-orcid":false,"given":"Yhu-Chering","family":"Huang","sequence":"additional","affiliation":[{"name":"College of Medicine, Chang Gung University, No. 261, Wenhua 1st Rd., Kweishan, Taoyuan 333, Taiwan"},{"name":"Division of Pediatric Infectious Diseases, Department of Pediatrics, Chang Gung Memorial Hospital, Linkou Medical Center, No. 5 Fu-Hsin Rd., Kweishan, Taoyuan 333, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,10,3]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"593","DOI":"10.1001\/jama.279.8.593","article-title":"Community-acquired methicillin-resistant Staphylococcus aureus in children with no identified predisposing risk","volume":"279","author":"Herold","year":"1998","journal-title":"JAMA"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"S269","DOI":"10.1086\/430788","article-title":"Community-associated methicillin-resistant Staphylococcus aureus","volume":"41","author":"Weber","year":"2005","journal-title":"Clin. 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