{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T21:12:17Z","timestamp":1780089137587,"version":"3.54.0"},"reference-count":40,"publisher":"MDPI AG","issue":"19","license":[{"start":{"date-parts":[[2022,10,5]],"date-time":"2022-10-05T00:00:00Z","timestamp":1664928000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Natural Science Foundation of China","award":["U21A20262"],"award-info":[{"award-number":["U21A20262"]}]},{"name":"National Natural Science Foundation of China","award":["22ZY1QA004"],"award-info":[{"award-number":["22ZY1QA004"]}]},{"name":"National Natural Science Foundation of China","award":["18YF1NA074"],"award-info":[{"award-number":["18YF1NA074"]}]},{"name":"National Natural Science Foundation of China","award":["20JR10RA563"],"award-info":[{"award-number":["20JR10RA563"]}]},{"name":"Central Guidance for Local Science and Technology Development Projects","award":["U21A20262"],"award-info":[{"award-number":["U21A20262"]}]},{"name":"Central Guidance for Local Science and Technology Development Projects","award":["22ZY1QA004"],"award-info":[{"award-number":["22ZY1QA004"]}]},{"name":"Central Guidance for Local Science and Technology Development Projects","award":["18YF1NA074"],"award-info":[{"award-number":["18YF1NA074"]}]},{"name":"Central Guidance for Local Science and Technology Development Projects","award":["20JR10RA563"],"award-info":[{"award-number":["20JR10RA563"]}]},{"name":"Gansu Province Key Research and Development Program Agriculture","award":["U21A20262"],"award-info":[{"award-number":["U21A20262"]}]},{"name":"Gansu Province Key Research and Development Program Agriculture","award":["22ZY1QA004"],"award-info":[{"award-number":["22ZY1QA004"]}]},{"name":"Gansu Province Key Research and Development Program Agriculture","award":["18YF1NA074"],"award-info":[{"award-number":["18YF1NA074"]}]},{"name":"Gansu Province Key Research and Development Program Agriculture","award":["20JR10RA563"],"award-info":[{"award-number":["20JR10RA563"]}]},{"name":"Gansu Key Laboratory of Animal Generational Physiology and Reproductive Regulation","award":["U21A20262"],"award-info":[{"award-number":["U21A20262"]}]},{"name":"Gansu Key Laboratory of Animal Generational Physiology and Reproductive Regulation","award":["22ZY1QA004"],"award-info":[{"award-number":["22ZY1QA004"]}]},{"name":"Gansu Key Laboratory of Animal Generational Physiology and Reproductive Regulation","award":["18YF1NA074"],"award-info":[{"award-number":["18YF1NA074"]}]},{"name":"Gansu Key Laboratory of Animal Generational Physiology and Reproductive Regulation","award":["20JR10RA563"],"award-info":[{"award-number":["20JR10RA563"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Hydrogen sulfide (H2S), as an endogenous gaseous signaling molecule, plays an important role in the inflammatory process. Our previous study found that Cystathionine-\u03b3-lyase (CTH) and H2S are correlated with the occurrence and development of Clinical Mastitis (CM) in Holstein cows. However, the functions and regulatory mechanisms of CTH\/H2S are still unknown. In this study, the inflammatory mammary cell model based on the MAC-T cell line was established by Lipopolysaccharide (LPS)-induced manner to further explore the function and regulatory mechanism of CTH\/H2S in cows with CM. In the inflammatory MAC-T cell, the CTH expression and H2S production were both repressed in an LPS-dose dependent manner, which demonstrated that CTH\/H2S is related to the progression of inflammation. The inhibition of CTH\/H2S using a selective CTH inhibitor, \u03b2-cyano-l-Alanine (BCA), promoted LPS-induced inflammation response and the expression of inflammatory cytokines. However, this was reversed by the H2S donor NaHS, demonstrating that H2S can protect cells from inflammatory damage. Intriguingly, interleukin-8 (IL-8) showed an inverse expression pattern correlated with the H2S-mediated cell protection effect during the inflammation process, and the inhibition test using a selective IL-8 receptor antagonist, SB225002, showed that IL-8 signaling plays a critical role in mediating endogenous H2S synthesis, and CTH\/H2S exerts its anti-inflammation via IL-8-mediated signaling. This study provided support for the prevention and treatment of CM and the development of a novel anti-inflammatory strategy.<\/jats:p>","DOI":"10.3390\/ijms231911822","type":"journal-article","created":{"date-parts":[[2022,10,8]],"date-time":"2022-10-08T23:39:31Z","timestamp":1665272371000},"page":"11822","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["CTH\/H2S Regulates LPS-Induced Inflammation through IL-8 Signaling in MAC-T Cells"],"prefix":"10.3390","volume":"23","author":[{"given":"Ting","family":"Lin","sequence":"first","affiliation":[{"name":"College of Life Science and Biotechnology, Gansu Agricultural University, Lanzhou 730070, China"},{"name":"Gansu Key Laboratory of Animal Generational Physiology and Reproductive Regulation, Lanzhou 730070, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xu","family":"Bai","sequence":"additional","affiliation":[{"name":"College of Life Science and Biotechnology, Gansu Agricultural University, Lanzhou 730070, China"},{"name":"Gansu Key Laboratory of Animal Generational Physiology and Reproductive Regulation, Lanzhou 730070, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6149-5487","authenticated-orcid":false,"given":"Yuan","family":"Gao","sequence":"additional","affiliation":[{"name":"College of Life Science and Biotechnology, Gansu Agricultural University, Lanzhou 730070, China"},{"name":"Gansu Key Laboratory of Animal Generational Physiology and Reproductive Regulation, Lanzhou 730070, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1064-7222","authenticated-orcid":false,"given":"Bohao","family":"Zhang","sequence":"additional","affiliation":[{"name":"Gansu Key Laboratory of Animal Generational Physiology and Reproductive Regulation, Lanzhou 730070, China"},{"name":"College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Shi","sequence":"additional","affiliation":[{"name":"College of Life Science and Biotechnology, Gansu Agricultural University, Lanzhou 730070, China"},{"name":"Gansu Key Laboratory of Animal Generational Physiology and Reproductive Regulation, Lanzhou 730070, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bao","family":"Yuan","sequence":"additional","affiliation":[{"name":"College of Life Science and Biotechnology, Gansu Agricultural University, Lanzhou 730070, China"},{"name":"Gansu Key Laboratory of Animal Generational Physiology and Reproductive Regulation, Lanzhou 730070, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenli","family":"Chen","sequence":"additional","affiliation":[{"name":"College of Life Science and Biotechnology, Gansu Agricultural University, Lanzhou 730070, China"},{"name":"Gansu Key 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China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5476-508X","authenticated-orcid":false,"given":"Quanwei","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Life Science and Biotechnology, Gansu Agricultural University, Lanzhou 730070, China"},{"name":"Gansu Key Laboratory of Animal Generational Physiology and Reproductive Regulation, Lanzhou 730070, China"},{"name":"College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xingxu","family":"Zhao","sequence":"additional","affiliation":[{"name":"College of Life Science and Biotechnology, Gansu Agricultural University, Lanzhou 730070, China"},{"name":"Gansu Key Laboratory of Animal Generational Physiology and Reproductive Regulation, Lanzhou 730070, China"},{"name":"College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,10,5]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1017\/S1466252319000094","article-title":"Causes, types, etiological agents, prevalence, diagnosis, treatment, prevention, effects on human health and future aspects of bovine mastitis","volume":"21","author":"Ashraf","year":"2020","journal-title":"Anim. 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