{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T21:12:56Z","timestamp":1787951576340,"version":"build-2784847793"},"reference-count":54,"publisher":"MDPI AG","issue":"20","license":[{"start":{"date-parts":[[2022,10,12]],"date-time":"2022-10-12T00:00:00Z","timestamp":1665532800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Spanish Ministerio de Econom\u00eda, Industria y Competitividad","award":["BIO2015-64169-P"],"award-info":[{"award-number":["BIO2015-64169-P"]}]},{"name":"Spanish Ministerio de Econom\u00eda, Industria y Competitividad","award":["US-1380339"],"award-info":[{"award-number":["US-1380339"]}]},{"name":"Universidad de Sevilla","award":["BIO2015-64169-P"],"award-info":[{"award-number":["BIO2015-64169-P"]}]},{"name":"Universidad de Sevilla","award":["US-1380339"],"award-info":[{"award-number":["US-1380339"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>In the diatom Phaeodactylum tricornutum, iron limitation promotes a decrease in the content of photosystem II, as determined by measurements of oxygen-evolving activity, thermoluminescence, chlorophyll fluorescence analyses and protein quantification methods. Thermoluminescence experiments also indicate that iron limitation induces subtle changes in the energetics of the recombination reaction between reduced QB and the S2\/S3 states of the water-splitting machinery. However, electron transfer from QA to QB, involving non-heme iron, seems not to be significantly inhibited. Moreover, iron deficiency promotes a severe decrease in the content of the extrinsic PsbV\/cytochrome c550 subunit of photosystem II, which appears in eukaryotic algae from the red photosynthetic lineage (including diatoms) but is absent in green algae and plants. The decline in the content of cytochrome c550 under iron-limiting conditions is accompanied by a decrease in the binding of this protein to photosystem II, and also of the extrinsic PsbO subunit. We propose that the lack of cytochrome c550, induced by iron deficiency, specifically affects the binding of other extrinsic subunits of photosystem II, as previously described in cyanobacterial PsbV mutants.<\/jats:p>","DOI":"10.3390\/ijms232012138","type":"journal-article","created":{"date-parts":[[2022,10,12]],"date-time":"2022-10-12T05:31:18Z","timestamp":1665552678000},"page":"12138","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Iron Deficiency Promotes the Lack of Photosynthetic Cytochrome c550 and Affects the Binding of the Luminal Extrinsic Subunits to Photosystem II in the Diatom Phaeodactylum tricornutum"],"prefix":"10.3390","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0920-4718","authenticated-orcid":false,"given":"Carmen","family":"Castell","sequence":"first","affiliation":[{"name":"Instituto de Bioqu\u00edmica Vegetal y Fotos\u00edntesis, cicCartuja, Universidad de Sevilla and CSIC, 41092 Seville, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6506-8303","authenticated-orcid":false,"given":"Encarnaci\u00f3n","family":"D\u00edaz-Santos","sequence":"additional","affiliation":[{"name":"Instituto de Bioqu\u00edmica Vegetal y Fotos\u00edntesis, cicCartuja, Universidad de Sevilla and CSIC, 41092 Seville, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luis G.","family":"Heredia-Mart\u00ednez","sequence":"additional","affiliation":[{"name":"Instituto de Bioqu\u00edmica Vegetal y Fotos\u00edntesis, cicCartuja, Universidad de Sevilla and CSIC, 41092 Seville, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3510-0621","authenticated-orcid":false,"given":"Luis","family":"L\u00f3pez-Maury","sequence":"additional","affiliation":[{"name":"Instituto de Bioqu\u00edmica Vegetal y Fotos\u00edntesis, cicCartuja, Universidad de Sevilla and CSIC, 41092 Seville, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 M.","family":"Ortega","sequence":"additional","affiliation":[{"name":"Instituto de Bioqu\u00edmica Vegetal y Fotos\u00edntesis, cicCartuja, Universidad de Sevilla and CSIC, 41092 Seville, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0536-6074","authenticated-orcid":false,"given":"Jos\u00e9 A.","family":"Navarro","sequence":"additional","affiliation":[{"name":"Instituto de Bioqu\u00edmica Vegetal y Fotos\u00edntesis, cicCartuja, Universidad de Sevilla and CSIC, 41092 Seville, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8749-7432","authenticated-orcid":false,"given":"Mercedes","family":"Roncel","sequence":"additional","affiliation":[{"name":"Instituto de Bioqu\u00edmica Vegetal y Fotos\u00edntesis, cicCartuja, Universidad de Sevilla and CSIC, 41092 Seville, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4523-8891","authenticated-orcid":false,"given":"Manuel","family":"Herv\u00e1s","sequence":"additional","affiliation":[{"name":"Instituto de Bioqu\u00edmica Vegetal y Fotos\u00edntesis, cicCartuja, Universidad de Sevilla and CSIC, 41092 Seville, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,10,12]]},"reference":[{"key":"ref_1","first-page":"785","article-title":"Electron transfer between membrane complexes and soluble proteins in photosynthesis","volume":"35","author":"Navarro","year":"2003","journal-title":"Acc. Chem. Res."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"43","DOI":"10.3389\/fmicb.2012.00043","article-title":"Iron utilization in marine cyanobacteria and eukaryotic algae","volume":"3","author":"Morrisey","year":"2012","journal-title":"Front. Microbiol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1016\/B978-0-12-394313-2.00003-2","article-title":"Iron in Cyanobacteria","volume":"Volume 65","author":"Chauvat","year":"2013","journal-title":"Genomics of Cyanobacteria"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"755","DOI":"10.1111\/j.0022-3646.1993.00755.x","article-title":"Response of the photosynthetic apparatus of Phaeodactylum tricornutum (Bacillariophyceae) to nitrate, phosphate, or iron starvation","volume":"29","author":"Geider","year":"1993","journal-title":"J. Phycol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"463","DOI":"10.1016\/S0967-0645(01)00109-6","article-title":"Iron cycling and nutrient limitation patterns in surface waters of the world ocean","volume":"49","author":"Moore","year":"2002","journal-title":"Deep Sea Res. Part II"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1082","DOI":"10.1093\/pcp\/pcab044","article-title":"New insights into the evolution of the electron transfer from cytochrome f to photosystem I in the green and red branches of photosynthetic eukaryotes","volume":"62","author":"Castell","year":"2021","journal-title":"Plant Cell Physiol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"10438","DOI":"10.1073\/pnas.0711370105","article-title":"Whole-cell response of the pennate diatom Phaeodactylum tricornutum to iron starvation","volume":"105","author":"Allen","year":"2008","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Nunn, B.L., Faux, J.F., Hippmann, A.A., Maldonado, M.T., Harvey, H.R., Goodlett, D.R., Boyd, P.W., and Strzepek, R.F. (2013). Diatom proteomics reveals unique acclimation strategies to mitigate Fe limitation. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0075653"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1050","DOI":"10.3389\/fpls.2016.01050","article-title":"Iron deficiency induces a partial inhibition of the photosynthetic electron transport and a high sensitivity to light in the diatom Phaeodactylum tricornutum","volume":"7","author":"Roncel","year":"2016","journal-title":"Front. Plant Sci."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1111\/ppl.13290","article-title":"The heterologous expression of a plastocyanin in the diatom Phaeodactylum tricornutum improves cell growth under iron-deficient conditions","volume":"171","author":"Castell","year":"2021","journal-title":"Physiol. Plant."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1146\/annurev-arplant-050312-120129","article-title":"The structure of photosystem II and the mechanism of water oxidation in photosynthesis","volume":"66","author":"Shen","year":"2015","journal-title":"Annu. Rev. Plant Biol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1261","DOI":"10.3389\/fpls.2015.01261","article-title":"The roles of cytochrome b559 in assembly and photoprotection of photosystem II revealed by site-directed mutagenesis studies","volume":"6","author":"Chu","year":"2016","journal-title":"Front. Plant Sci."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1007\/s11120-011-9628-2","article-title":"Inhibition of photosynthetic oxygen evolution and electron transfer from the quinone acceptor QA\u2212 to QB by iron deficiency","volume":"107","author":"Msilini","year":"2011","journal-title":"Photosynth. Res."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1007\/s11120-013-9926-y","article-title":"The nonheme iron in photosystem II","volume":"116","author":"Zouni","year":"2013","journal-title":"Photosynth. Res."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"820","DOI":"10.1093\/pcp\/pcg106","article-title":"Comparison of binding and functional properties of two extrinsic components, cyt c550 and a 12 kDa protein, in cyanobacterial PSII with those in red algal PSII","volume":"44","author":"Enami","year":"2003","journal-title":"Plant Cell Physiol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"29191","DOI":"10.1074\/jbc.M110.146092","article-title":"Binding and functional properties of five extrinsic proteins in oxygen-evolving photosystem II from a marine centric diatom, Chaetoceros gracilis","volume":"285","author":"Nagao","year":"2010","journal-title":"J. Biol. Chem."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"eaax4406","DOI":"10.1126\/science.aax4406","article-title":"The pigment-protein network of a diatom photosystem II-light harvesting antenna supercomplex","volume":"365","author":"Pi","year":"2019","journal-title":"Science"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1038\/nature09913","article-title":"Crystal structure of oxygen-evolving photosystem II at a resolution of 1.9 \u00c5","volume":"473","author":"Umena","year":"2011","journal-title":"Nature"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1152","DOI":"10.1016\/j.bbabio.2012.01.008","article-title":"Photosynthetic cytochrome c550","volume":"1817","author":"Roncel","year":"2012","journal-title":"Biochim. Biophys. Acta-Bioenerg."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1007\/s11120-008-9343-9","article-title":"Structures and functions of the extrinsic proteins of photosystem II from different species","volume":"98","author":"Enami","year":"2008","journal-title":"Photosynth. Res."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"5676","DOI":"10.1074\/jbc.M115.711689","article-title":"Novel features of eukaryotic photosystem II revealed by its crystal structure analysis from a red alga","volume":"291","author":"Ago","year":"2016","journal-title":"J. Biol. Chem."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1007\/s11120-016-0327-x","article-title":"The photosynthetic cytochrome c550 from the diatom Phaeodactylum tricornutum","volume":"133","author":"Yruela","year":"2017","journal-title":"Photosynth. Res."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"84","DOI":"10.3389\/fpls.2016.00084","article-title":"Structural coupling of extrinsic proteins with the oxygen-evolving center in photosystem II","volume":"7","author":"Ifuku","year":"2016","journal-title":"Front. Plant Sci."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"890","DOI":"10.1038\/s41477-019-0477-x","article-title":"Structural basis for energy harvesting and dissipation in a diatom PSII-FCPII supercomplex","volume":"5","author":"Nagao","year":"2019","journal-title":"Nat. Plants"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1825","DOI":"10.1021\/bi00058a017","article-title":"Binding and functional properties of two new extrinsic components, cytochrome c550 and a 12-kDa protein, in cyanobacterial photosystem II","volume":"32","author":"Shen","year":"1993","journal-title":"Biochemistry"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"2787","DOI":"10.1021\/bi9724624","article-title":"Binding and functional properties of four extrinsic proteins of photosystem II from a red alga, Cyanidium caldarium, as studied by release-reconstitution experiments","volume":"37","author":"Enami","year":"1998","journal-title":"Biochemistry"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1551","DOI":"10.1021\/bi971676i","article-title":"Functional characterization of Synechocystis sp. PCC 6803 \u0394psbU and \u0394psbV mutants reveals important roles of cytochrome c-550 in cyanobacterial oxygen evolution","volume":"37","author":"Shen","year":"1998","journal-title":"Biochemistry"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1016\/j.bbabio.2011.07.006","article-title":"The extrinsic proteins of Photosystem II","volume":"1817","author":"Bricker","year":"2012","journal-title":"Biochim. Biophys. Acta-Bioenerg."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1007\/s11120-020-00753-8","article-title":"Role of PsbV-Tyr137 in photosystem II studied by site-directed mutagenesis in the thermophilic cyanobacterium Thermosynechococcus vulcanus","volume":"146","author":"Xiao","year":"2020","journal-title":"Photosynth. Res."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"52869","DOI":"10.1074\/jbc.M408206200","article-title":"Cytochrome c550 in the cyanobacterium Thermosynechococcus elongatus: Study of redox mutants","volume":"279","author":"Kirilovsky","year":"2004","journal-title":"J. Biol. Chem."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"14161","DOI":"10.1021\/bi0486738","article-title":"In situ effects of mutations of the extrinsic cytochrome c550 of photosystem II in Synechocystis sp. PCC6803","volume":"43","author":"Li","year":"2004","journal-title":"Biochemistry"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"152","DOI":"10.1016\/j.cbpa.2014.12.040","article-title":"Oxygen-evolving complex of Photosystem II: An analysis of second-shell residues and hydrogen-bonding networks","volume":"25","author":"Vogt","year":"2015","journal-title":"Curr. Opin. Chem. Biol."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Smith, S.R., Gillard, J.T., Kustka, A.B., McCrow, J.P., Badger, J.H., Zheng, H., New, A.M., Dupont, C.L., Obata, T., and Fernie, A.R. (2016). Transcriptional Orchestration of the Global Cellular Response of a Model Pennate Diatom to Diel Light Cycling under Iron Limitation. PLoS Genet., 12.","DOI":"10.1371\/journal.pgen.1006490"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"268","DOI":"10.1111\/ppl.13288","article-title":"The afterglow photosynthetic luminescence","volume":"171","author":"Ortega","year":"2021","journal-title":"Physiol. Plant."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"445","DOI":"10.1007\/978-94-007-1579-0_19","article-title":"Thermoluminescence","volume":"Volume 34","author":"Tripathy","year":"2012","journal-title":"Photosynthesis: Plastid Biology, Energy Conversion and Carbon Assimilation"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"548","DOI":"10.1016\/0005-2728(84)90054-9","article-title":"Thermoluminiscence as a probe of photosystem II. The redox and protonation states of the secondary acceptor quinone and O2-evolving enzyme","volume":"767","author":"Rutherford","year":"1984","journal-title":"Biochim. Biophys. Acta-Bioenerg."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"6137","DOI":"10.1021\/bi00186a013","article-title":"Site-directed photosystem II mutants with perturbed oxygen-evolving properties. 1. Instability or inefficient assembly of the manganese cluster in vivo","volume":"33","author":"Chu","year":"1994","journal-title":"Biochemistry"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"686","DOI":"10.1016\/j.bbabio.2007.02.016","article-title":"Site-directed mutagenesis on the heme axial-ligands of cytochrome b559 in photosystem II by using cyanobacteria Synechocystis PCC 6803","volume":"1767","author":"Hung","year":"2007","journal-title":"Biochim. Biophys. Acta-Bioenerg."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"E317","DOI":"10.1073\/pnas.1118408109","article-title":"Comparative metatranscriptomics identifies molecular bases for the physiological responses of phytoplankton to varying iron availability","volume":"109","author":"Marchetti","year":"2012","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"565","DOI":"10.1104\/pp.100.2.565","article-title":"Iron-induced changes in light harvesting and photochemical energy conversion processes in eukaryotic marine algae","volume":"100","author":"Greene","year":"1992","journal-title":"Plant Physiol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1016\/0005-2728(93)90134-2","article-title":"Photoinhibition of photosystem II. Inactivation, protein damage and turnover","volume":"1143","author":"Aro","year":"1993","journal-title":"Biochim. Biophys. Acta"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1007\/s11120-022-00912-z","article-title":"From manganese oxidation to water oxidation: Assembly and evolution of the water-splitting complex in photosystem II","volume":"152","author":"Oliver","year":"2022","journal-title":"Photosynth. Res."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"769","DOI":"10.1139\/m64-098","article-title":"Some considerations of the growth of marine algae in artificial media","volume":"10","author":"McLachlan","year":"1964","journal-title":"Can. J. Microbiol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"695","DOI":"10.4319\/lo.1978.23.4.0695","article-title":"Steady state growth and ammonium uptake of a fast-growing marine diatom","volume":"23","author":"Goldman","year":"1978","journal-title":"Limnol. Oceanogr."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"510","DOI":"10.1046\/j.1529-8817.1999.3530510.x","article-title":"Accumulation of ferredoxin and flavodoxin in a marine diatom in response to Fe","volume":"35","author":"McKay","year":"1999","journal-title":"J. Phycol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1111\/gcb.13501","article-title":"Decreased photosynthesis and growth with reduced respiration in the model diatom Phaeodactylum tricornutum grown under elevated CO2 over 1800 generations","volume":"23","author":"Li","year":"2017","journal-title":"Glob. Change Biol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1016\/S0021-9258(19)52451-6","article-title":"Protein measurement with the Folin phenol reagent","volume":"193","author":"Lowry","year":"1951","journal-title":"J. Biol. Chem."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"206","DOI":"10.1016\/0003-2697(78)90586-9","article-title":"A modification of the Lowry procedure to simplify protein determination in membrane and lipoprotein samples","volume":"87","author":"Markwell","year":"1978","journal-title":"Anal. Biochem."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1006\/abbi.1995.1202","article-title":"Purification and physiochemical properties of the low potential cytochrome c549 from the cyanobacterium Synechocystis sp. PCC 6803","volume":"318","author":"Navarro","year":"1995","journal-title":"Arch. Biochem. Biophys."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"1549","DOI":"10.1016\/j.bbabio.2015.09.006","article-title":"Interaction of photosystem I from Phaeodactylum tricornutum with plastocyanins as compared with its native cytochrome c6: Reunion with a lost donor","volume":"1847","author":"Pallara","year":"2015","journal-title":"Biochim. Biophys. Acta-Bioenerg."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"240","DOI":"10.1111\/ppl.12916","article-title":"The afterglow thermoluminescence band as an indicator of changes in the photorespiratory metabolism of the model legume Lotus japonicus","volume":"166","author":"Betti","year":"2019","journal-title":"Physiol. Plant."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1007\/BF00032979","article-title":"Graphical and numerical analysis of thermoluminescence and fluorescence F0 emission in photosynthetic material","volume":"33","author":"Ducruet","year":"1992","journal-title":"Photosynth. Res."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1007\/s11120-004-6427-z","article-title":"A thermoluminescence study of photosystem II back electron transfer reactions in rice leaves. Effects of salt stress","volume":"84","author":"Zurita","year":"2005","journal-title":"Photosynth. Res."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1016\/j.jphotobiol.2011.02.014","article-title":"Peculiar properties of chlorophyll thermoluminescence emission of autotrophically or mixotrophically grown Chlamydomonas reinhardtii","volume":"104","author":"Ducruet","year":"2011","journal-title":"J. Photochem. Photobiol. B Biol."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/23\/20\/12138\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T00:50:22Z","timestamp":1760143822000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/23\/20\/12138"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,10,12]]},"references-count":54,"journal-issue":{"issue":"20","published-online":{"date-parts":[[2022,10]]}},"alternative-id":["ijms232012138"],"URL":"https:\/\/doi.org\/10.3390\/ijms232012138","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,10,12]]}}}