{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T16:19:58Z","timestamp":1791476398228,"version":"4.3.4"},"reference-count":51,"publisher":"MDPI AG","issue":"20","license":[{"start":{"date-parts":[[2022,10,12]],"date-time":"2022-10-12T00:00:00Z","timestamp":1665532800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Shandong Provincial Natural Science Foundations, China","award":["ZR2019MC043"],"award-info":[{"award-number":["ZR2019MC043"]}]},{"name":"Shandong Provincial Natural Science Foundations, China","award":["ZR2020QC136"],"award-info":[{"award-number":["ZR2020QC136"]}]},{"name":"Shandong Provincial Natural Science Foundations, China","award":["2021CXGC010811"],"award-info":[{"award-number":["2021CXGC010811"]}]},{"name":"Shandong Provincial Natural Science Foundations, China","award":["2021YFD1700101"],"award-info":[{"award-number":["2021YFD1700101"]}]},{"name":"Key R&amp;D Program of Shandong Province","award":["ZR2019MC043"],"award-info":[{"award-number":["ZR2019MC043"]}]},{"name":"Key R&amp;D Program of Shandong Province","award":["ZR2020QC136"],"award-info":[{"award-number":["ZR2020QC136"]}]},{"name":"Key R&amp;D Program of Shandong Province","award":["2021CXGC010811"],"award-info":[{"award-number":["2021CXGC010811"]}]},{"name":"Key R&amp;D Program of Shandong Province","award":["2021YFD1700101"],"award-info":[{"award-number":["2021YFD1700101"]}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["ZR2019MC043"],"award-info":[{"award-number":["ZR2019MC043"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["ZR2020QC136"],"award-info":[{"award-number":["ZR2020QC136"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2021CXGC010811"],"award-info":[{"award-number":["2021CXGC010811"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2021YFD1700101"],"award-info":[{"award-number":["2021YFD1700101"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Beckmannia syzigachne is a noxious grassy weed that infests wheat fields in China. Previously, we identified that mesosulfuron-methyl resistance in a B. syzigachne population (R, SD04) was conferred by non-target resistance, such as cytochrome P450 mixed-function oxidases (P450s)-based metabolism. RNA sequencing and real-time PCR (qRT-PCR) were used to discover potential P450s-resistant-related genes. Five cytochrome P450s (CYP704A177, CYP96B84, CYP71D7, CYP93A1, and CYP99A44) were found to be highly expressed in R plants. In this study, CYP99A44 and CYP704A177 were cloned from B. syzigachne and transferred into Arabidopsis thaliana to test the sensitivity of Arabidopsis with and without P450s genes to mesosulfuron-methyl and other acetolactate synthase (ALS)-inhibiting herbicides. Transgenic Arabidopsis overexpressing CYP99A44 became resistant to the sulfonylurea herbicide mesosulfuron-methyl, but showed no resistance to pyroxsulam, imazethapyr, flucarbazone, and bispyribac-sodium. Notably, those overexpressing CYP704A177 showed resistance to pyroxsulam and bispyribac-sodium, but not to mesosulfuron-methyl, imazethapyr, and flucarbazone. These results indicated that B. syzigachne and transgenic Arabidopsis displayed different cross-resistance patterns to ALS-inhibiting herbicides. Subcellular localization revealed that CYP99A44 and CYP704A177 protein were located in the endoplasmic reticulum. Furthermore, these results clearly indicated that CYP99A44-mediated mesosulfuron-methyl resistance in B. syzigachne and CYP704A177 may be involved in B. syzigachne cross-resistance to pyroxsulam and bispyribac-sodium.<\/jats:p>","DOI":"10.3390\/ijms232012175","type":"journal-article","created":{"date-parts":[[2022,10,12]],"date-time":"2022-10-12T20:53:29Z","timestamp":1665608009000},"page":"12175","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":38,"title":["Cytochrome P450 BsCYP99A44 and BsCYP704A177 Confer Metabolic Resistance to ALS Herbicides in Beckmannia syzigachne"],"prefix":"10.3390","volume":"23","author":[{"given":"Shuang","family":"Bai","sequence":"first","affiliation":[{"name":"College of Plant Health and Medicine, Qingdao Agricultural University, Qingdao 266109, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mengjie","family":"Yin","sequence":"additional","affiliation":[{"name":"College of Plant Health and Medicine, Qingdao Agricultural University, Qingdao 266109, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qinghao","family":"Lyu","sequence":"additional","affiliation":[{"name":"College of Plant Health and Medicine, Qingdao Agricultural University, Qingdao 266109, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bo","family":"Jiang","sequence":"additional","affiliation":[{"name":"College of Plant Health and Medicine, Qingdao Agricultural University, Qingdao 266109, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1436-0069","authenticated-orcid":false,"given":"Lingxu","family":"Li","sequence":"additional","affiliation":[{"name":"College of Plant Health and Medicine, Qingdao Agricultural University, Qingdao 266109, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,10,12]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"728","DOI":"10.1126\/science.aar3780","article-title":"Wicked evolution: Can we address the sociobiological dilemma of pesticide resistance?","volume":"360","author":"Gould","year":"2018","journal-title":"Science"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"611","DOI":"10.1146\/annurev-arplant-042817-040038","article-title":"Population genomics of herbicide resistance: Adaptation via evolutionary rescue","volume":"69","author":"Kreiner","year":"2018","journal-title":"Annu. 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