{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,21]],"date-time":"2026-02-21T07:24:56Z","timestamp":1771658696465,"version":"3.50.1"},"reference-count":41,"publisher":"MDPI AG","issue":"20","license":[{"start":{"date-parts":[[2022,10,16]],"date-time":"2022-10-16T00:00:00Z","timestamp":1665878400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"MacKay Memorial Hospital","award":["10940"],"award-info":[{"award-number":["10940"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Few studies have examined the correlation between sperm miRNA levels and clinical outcomes of intracytoplasmic sperm injection (ICSI). In this study, we aimed to assess the correlation of sperm miR-34b, miR-34c, miR-122, and miR-429 levels with ICSI outcomes in men with teratozoospermia and asthenozoospermia. TaqMan microRNA quantitative polymerase chain reaction was used to evaluate the relative expression of miRNAs in sperm. The relative miRNA levels quantified using a comparative method found that the four miRNAs were not associated with fertilization rate and early embryo development. However, revels of miR-34b and miR-34c in teratozoospermia sperm of the live birth group were significantly higher than those in the non-live birth group. Receiver operating characteristic curve analysis revealed that the optimal cut-off delta cycle threshold values of miR-34b and miR-34c were 8.630 and 7.883, respectively. Statistical analysis found that the levels of miR-34b and the miR-34c in teratozoospermic and asthenozoospermic sperm above the thresholds were not associated with the fertilization rate and the high-quality embryo rate above 50%; however, they were more likely to exhibit higher implantation, pregnancy, and live birth rates. miR-34b and miR-34c were significantly associated with ICSI clinical outcomes in male factor infertility, especially teratozoospermia. Further validation is required before it becomes a clinically valid reference indicator.<\/jats:p>","DOI":"10.3390\/ijms232012381","type":"journal-article","created":{"date-parts":[[2022,10,17]],"date-time":"2022-10-17T02:29:46Z","timestamp":1665973786000},"page":"12381","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Correlation between Sperm Micro Ribonucleic Acid-34b and -34c Levels and Clinical Outcomes of Intracytoplasmic Sperm Injection in Men with Male Factor Infertility"],"prefix":"10.3390","volume":"23","author":[{"given":"Ling-Yu","family":"Yeh","sequence":"first","affiliation":[{"name":"Institute of Biochemical and Biomedical Engineering, Department of Chemical Engineering & Biotechnology, National Taipei University of Technology, Taipei 106, Taiwan"},{"name":"Department of Medical Research, MacKay Memorial Hospital, Tamsui District, New Taipei 251, Taiwan"},{"name":"MacKay Junior College of Medicine, Nursing, and Management, Taipei 112, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert Kuo-Kuang","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Medical Research, MacKay Memorial Hospital, Tamsui District, New Taipei 251, Taiwan"},{"name":"Department of Obstetrics and Gynecology, MacKay Memorial Hospital, Taipei 104, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ming-Huei","family":"Lin","sequence":"additional","affiliation":[{"name":"MacKay Junior College of Medicine, Nursing, and Management, Taipei 112, Taiwan"},{"name":"Department of Obstetrics and Gynecology, MacKay Memorial Hospital, Taipei 104, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chih-Hung","family":"Huang","sequence":"additional","affiliation":[{"name":"Institute of Biochemical and Biomedical Engineering, Department of Chemical Engineering & Biotechnology, National Taipei University of Technology, Taipei 106, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0549-3516","authenticated-orcid":false,"given":"Sheng-Hsiang","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Medical Research, MacKay Memorial Hospital, Tamsui District, New Taipei 251, Taiwan"},{"name":"MacKay Junior College of Medicine, Nursing, and Management, Taipei 112, Taiwan"},{"name":"Department of Chemical Engineering & Biotechnology, National Taipei University of Technology, Taipei 106, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,10,16]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1016\/j.cell.2009.01.002","article-title":"MicroRNAs: Target Recognition and Regulatory Functions","volume":"136","author":"Bartel","year":"2009","journal-title":"Cell"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"861","DOI":"10.1038\/nrg3074","article-title":"Non-Coding RNAs in Human Disease","volume":"12","author":"Esteller","year":"2011","journal-title":"Nat. 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