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Attraction and International Mobility, Linea 1 Mobilit\u00e0 dei ricercatori","award":["AIM1874325-3"],"award-info":[{"award-number":["AIM1874325-3"]}]},{"name":"Programma Operativo Nazionale (PON) Ricerca e Innovazione 2014\u20132020\u2014Asse I \u201cCapitale Umano\u201d, Azione I.2\u2014A.I.M. Attraction and International Mobility, Linea 1 Mobilit\u00e0 dei ricercatori","award":["CUP: J54I18000110001"],"award-info":[{"award-number":["CUP: J54I18000110001"]}]},{"name":"Programma Operativo Nazionale (PON) Ricerca e Innovazione 2014\u20132020\u2014Asse I \u201cCapitale Umano\u201d, Azione I.2\u2014A.I.M. Attraction and International Mobility, Linea 1 Mobilit\u00e0 dei ricercatori","award":["D.R. 2397\/2021"],"award-info":[{"award-number":["D.R. 2397\/2021"]}]},{"name":"Fondazione di Sardegna 2018\u20132020 and 2021\u2014Progetti di Ricerca di Base Dipartimentali","award":["AIM1874325-3"],"award-info":[{"award-number":["AIM1874325-3"]}]},{"name":"Fondazione di Sardegna 2018\u20132020 and 2021\u2014Progetti di Ricerca di Base Dipartimentali","award":["CUP: J54I18000110001"],"award-info":[{"award-number":["CUP: J54I18000110001"]}]},{"name":"Fondazione di Sardegna 2018\u20132020 and 2021\u2014Progetti di Ricerca di Base Dipartimentali","award":["D.R. 2397\/2021"],"award-info":[{"award-number":["D.R. 2397\/2021"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Carotid atherosclerosis represents a relevant healthcare problem, since unstable plaques are responsible for approximately 15% of neurologic events, namely transient ischemic attack and stroke. Although statins treatment has proven effective in reducing LDL-cholesterol and the onset of acute clinical events, a residual risk may persist suggesting the need for the detection of reliable molecular markers useful for the identification of patients at higher risk regardless of optimal medical therapy. In this regard, several lines of evidence show a relationship among specific biologically active plasma lipids, atherosclerosis, and acute clinical events. We performed a Selected Reaction Monitoring-based High Performance Liquid Chromatography-tandem Mass Spectrometry (SRM-based HPLC-MS\/MS) analysis on plasma HDL, LDL, and VLDL fractions purified, by isopycnic salt gradient ultracentrifugation, from twenty-eight patients undergoing carotid endarterectomy, having either a \u201chard\u201d or a \u201csoft\u201d plaque, with the aim of characterizing the specific lipidomic patterns associated with features of carotid plaque instability. One hundred and thirty lipid species encompassing different lipid (sub)classes were monitored. Supervised multivariate analysis showed that lipids belonging to phosphatidylethanolamine (PE), sphingomyelin (SM), and diacylglycerol (DG) classes mostly contribute to discrimination within each lipoprotein fraction according to the plaque typology. Differential analysis evidenced a significant dysregulation of LDL PE (38:6), SM (32:1), and SM (32:2) between the two groups of patients (adj. p-value threshold = 0.05 and log2FC \u2265 |0.58|). Using this approach, some LDL-associated markers of plaque vulnerability have been identified, in line with the current knowledge of the key roles of these phospholipids in lipoprotein metabolism and cardiovascular disease. This proof-of-concept study reports promising results, showing that lipoprotein lipidomics may present a valuable approach for identifying new biomarkers of potential clinical relevance.<\/jats:p>","DOI":"10.3390\/ijms232012449","type":"journal-article","created":{"date-parts":[[2022,10,18]],"date-time":"2022-10-18T21:18:02Z","timestamp":1666127882000},"page":"12449","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Molecular Characterization of Plasma HDL, LDL, and VLDL Lipids Cargos from Atherosclerotic Patients with Advanced Carotid Lesions: A Preliminary Report"],"prefix":"10.3390","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9268-9436","authenticated-orcid":false,"given":"Gabriele","family":"Nieddu","sequence":"first","affiliation":[{"name":"Department of Biomedical Sciences, University of Sassari, 07100 Sassari, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1619-401X","authenticated-orcid":false,"given":"Elena","family":"Michelucci","sequence":"additional","affiliation":[{"name":"Institute of Clinical Physiology, National Research Council, 56124 Pisa, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marilena","family":"Formato","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, University of Sassari, 07100 Sassari, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cristina","family":"Ciampelli","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, University of Sassari, 07100 Sassari, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3192-755X","authenticated-orcid":false,"given":"Gabriele","family":"Obino","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, University of Sassari, 07100 Sassari, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0067-2240","authenticated-orcid":false,"given":"Giovanni","family":"Signore","sequence":"additional","affiliation":[{"name":"Department of Biology, Biochemistry Unit, University of Pisa, 56126 Pisa, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicoletta","family":"Di Giorgi","sequence":"additional","affiliation":[{"name":"Institute of Clinical Physiology, National Research Council, 56124 Pisa, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3831-4200","authenticated-orcid":false,"given":"Silvia","family":"Rocchiccioli","sequence":"additional","affiliation":[{"name":"Institute of Clinical Physiology, National Research Council, 56124 Pisa, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8356-894X","authenticated-orcid":false,"given":"Antonio Junior","family":"Lepedda","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, University of Sassari, 07100 Sassari, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,10,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/j.atherosclerosis.2010.05.013","article-title":"Carotid plaque formation and serum biomarkers","volume":"213","author":"Hermus","year":"2010","journal-title":"Atherosclerosis"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1007\/s11883-018-0715-0","article-title":"The Interplay of Lipids, Lipoproteins, and Immunity in Atherosclerosis","volume":"20","author":"Pirillo","year":"2018","journal-title":"Curr. 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