{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,24]],"date-time":"2026-01-24T18:38:17Z","timestamp":1769279897267,"version":"3.49.0"},"reference-count":59,"publisher":"MDPI AG","issue":"20","license":[{"start":{"date-parts":[[2022,10,19]],"date-time":"2022-10-19T00:00:00Z","timestamp":1666137600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"funds from authorized direct expenditure to the sub-directorate of basic research, Institute National of Cardiology, Ignacio Ch\u00e1vez"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Deodorized garlic (DG) may favor the activity of the antioxidant enzymes and promote the synthesis of hydrogen sulfide (H2S). The objective was to test if DG favors an increase in H2S and if it decreases the oxidative stress caused by lipopolysaccharide (LPS) in rat hearts. A total of 24 rats were divided into 4 groups: Group 1 control (C), Group 2 LPS, Group 3 DG, and Group 4 LPS plus DG. The cardiac mechanical performance (CMP), coronary vascular resistance (CVR), and oxidative stress markers, such as total antioxidant capacity (TAC), glutathione (GSH), selenium (Se), lipid peroxidation (LPO), thiols, hydrogen sulfide (H2S), and the activities and expressions of thioredoxin reductase (TrxR), glutathione peroxidase (GPx), and glutathione-S-transferase (GST), cystathionine synthetase (CBS), cystathionine \u03b3-lyase (CTH), iNOS, and eNOS-p, were analyzed in the heart. Infarct zones in the cardiac tissue were present (p = 0.01). The CMP and CVR decreased and increased (p \u2264 0.05), TAC, GSH, H2S, NO, thiols, and GST activity (p \u2264 0.01) decreased, and LPO and iNOS increased (p \u2264 0.05). The activities and expressions of TrxR, GPx, eNOS-p, CTH, and CBS (p \u2264 0.05) decreased with the LPS treatment; however, DG normalized this effect. DG treatment decreases heart damage caused by LPS through the cross-talk between the H2S and NO systems.<\/jats:p>","DOI":"10.3390\/ijms232012529","type":"journal-article","created":{"date-parts":[[2022,10,19]],"date-time":"2022-10-19T20:32:23Z","timestamp":1666211543000},"page":"12529","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Deodorized Garlic Decreases Oxidative Stress Caused by Lipopolysaccharide in Rat Heart through Hydrogen Sulfide: Preliminary Findings"],"prefix":"10.3390","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6510-2954","authenticated-orcid":false,"given":"Israel","family":"P\u00e9rez-Torres","sequence":"first","affiliation":[{"name":"Department of Cardiovascular Biomedicine, Instituto Nacional de Cardiolog\u00eda Ignacio Ch\u00e1vez, Juan Badiano 1, Secci\u00f3n XVI, Tlalpan, Ciudad de M\u00e9xico 14080, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Linaloe","family":"Manzano-Pech","sequence":"additional","affiliation":[{"name":"Department of Cardiovascular Biomedicine, Instituto Nacional de Cardiolog\u00eda Ignacio Ch\u00e1vez, Juan Badiano 1, Secci\u00f3n XVI, Tlalpan, Ciudad de M\u00e9xico 14080, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ver\u00f3nica","family":"Guarner-Lans","sequence":"additional","affiliation":[{"name":"Department de and Hysiology, Instituto Nacional de Cardiolog\u00eda Ignacio Ch\u00e1vez, Juan Badiano 1, Secci\u00f3n XVI, Tlalpan, Ciudad de M\u00e9xico 14080, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda Elena","family":"Soto","sequence":"additional","affiliation":[{"name":"Department de Inmunology, Instituto Nacional de Cardiolog\u00eda Ignacio Ch\u00e1vez, Juan Badiano 1, Secci\u00f3n XVI, Tlalpan, M\u00e9xico City 14080, 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Anabell","family":"D\u00edaz-Ju\u00e1rez","sequence":"additional","affiliation":[{"name":"Department de Pharmacology, Instituto Nacional de Cardiolog\u00eda Ignacio Ch\u00e1vez, Juan Badiano 1, Secci\u00f3n XVI, Tlalpan, Ciudad de M\u00e9xico 14080, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6697-0208","authenticated-orcid":false,"given":"Mar\u00eda","family":"S\u00e1nchez-Aguilar","sequence":"additional","affiliation":[{"name":"Department de Pharmacology, Instituto Nacional de Cardiolog\u00eda Ignacio Ch\u00e1vez, Juan Badiano 1, Secci\u00f3n XVI, Tlalpan, Ciudad de M\u00e9xico 14080, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3669-7482","authenticated-orcid":false,"given":"Juan Carlos","family":"Torres-Narv\u00e1ez","sequence":"additional","affiliation":[{"name":"Department de Pharmacology, Instituto Nacional de Cardiolog\u00eda Ignacio Ch\u00e1vez, Juan Badiano 1, 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Nutr. Food. Res."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"148","DOI":"10.1016\/j.clnesp.2017.11.010","article-title":"Aged garlic extract supplementation modifies inflammation and immunity of adults with obesity: A randomized, double-blind, placebo-controlled clinical trial","volume":"24","author":"Xu","year":"2018","journal-title":"Clin. Nutr. ESPEN"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"563","DOI":"10.1016\/S0278-6915(00)00171-X","article-title":"Effects of organosulfur compounds from garlic oil on the antioxidation system in rat liver and red blood cells","volume":"39","author":"Wu","year":"2002","journal-title":"Food. Chem. Toxicol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1248","DOI":"10.1139\/Y07-120","article-title":"Hydrogen sulfide contributes to cardioprotection during ischemia-reperfusion injury by opening K ATP channels","volume":"85","author":"Zhang","year":"2007","journal-title":"Can. J. Physiol. Pharmacol."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Louis, X.L., Murphy, R., Thandapilly, S.J., Yu, L., and Netticadan, T. (2012). Garlic extracts prevent oxidative stress, hypertrophy and apoptosis in cardiomyocytes: A role for nitric oxide and hydrogen sulfide. BMC. Complement. Altern. Med., 12.","DOI":"10.1186\/1472-6882-12-140"},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Rodrigues, C., and Percival, S.S. (2019). Immunomodulatory effects of glutathione, garlic derivatives, and hydrogen sulfide. Nutrients, 11.","DOI":"10.3390\/nu11020295"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"539","DOI":"10.1146\/annurev.nutr.24.012003.132418","article-title":"Sulfur amino acid metabolism: Pathways for production and removal of homocysteine and cysteine","volume":"24","author":"Stipanuk","year":"2004","journal-title":"Annu. Rev. Nutr."},{"key":"ref_8","first-page":"5492127","article-title":"Oxidative stress in plasma from patients with marfan syndrome is modulated by deodorized garlic preliminary findings","volume":"2022","author":"Soto","year":"2022","journal-title":"Oxid. Med. Cell. Longev."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Aisa-Alvarez, A., Soto, M.E., Guarner-Lans, V., Camarena-Alejo, G., Franco-Granillo, J., Mart\u00ednez-Rodr\u00edguez, E.A., \u00c1vila, R.G., Manzano-Pech, L., and P\u00e9rez-Torres, I. (2020). Usefulness of antioxidants as adjuvant therapy for septic shock: A randomized clinical trial. Medicina, 56.","DOI":"10.3390\/medicina56110619"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1054","DOI":"10.1503\/cmaj.050632","article-title":"Early and innovative interventions for severe sepsis and septic shock: Taking advantage of a window of opportunity","volume":"173","author":"Rivers","year":"2005","journal-title":"CMAJ"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1699","DOI":"10.1056\/NEJMra043632","article-title":"Management of sepsis","volume":"355","author":"Russell","year":"2006","journal-title":"N. Engl. J. Med."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1062","DOI":"10.1016\/S0735-1097(98)00660-3","article-title":"Effects of endotoxin on human myocardial contractility involvement of nitric oxide and peroxynitrite","volume":"33","author":"Flesch","year":"1999","journal-title":"Am. Coll. Cardiol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1650","DOI":"10.1159\/000489241","article-title":"Current mechanistic concepts in ischemia and reperfusion injury","volume":"46","author":"Wu","year":"2018","journal-title":"Cell. Physiol. Biochem."},{"key":"ref_14","first-page":"381815","article-title":"Alliin, a garlic (Allium sativum) compound, prevents LPS-induced inflammation in 3T3-L1 adipocytes","volume":"2013","year":"2013","journal-title":"Mediat. Inflamm."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"401630","DOI":"10.1155\/2015\/401630","article-title":"Immunomodulation and anti-inflammatory effects of garlic compounds","volume":"2015","author":"Arreola","year":"2015","journal-title":"J. Immunol. Res."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"2837","DOI":"10.1089\/ars.2010.3713","article-title":"Lipopolysaccharide induced mitochondrial DNA depletion","volume":"15","author":"Choumar","year":"2011","journal-title":"Antioxid. Redox. Signal."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"P\u00e9rez-Torres, I., Torres-Narv\u00e1ez, J.C., Pedraza-Chaverri, J., Rubio-Ru\u00edz, M.E., D\u00edaz-D\u00edaz, E., Del Valle-Mondrag\u00f3n, L., Mart\u00ednez-Memije, R., Varela L\u00f3pez, E., and Guarner-Lans, V. (2016). Effect of the aged garlic extract on cardiovascular function in metabolic syndrome rats. Molecules, 21.","DOI":"10.3390\/molecules21111425"},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Shang, A., Cao, S.-Y., Xu, X.-Y., Gan, R.-Y., Tang, G.-Y., Corke, H., Mavumengwana, V., and Li, H.-B. (2019). Bioactive compounds and biological functions of garlic (Allium sativum L.). Foods, 8.","DOI":"10.3390\/foods8070246"},{"key":"ref_19","first-page":"4898","article-title":"LncRNA NEAT1 alleviates sepsis-induced myocardial injury by regulating the TLR2\/NF-\u03baB signaling pathway","volume":"23","author":"Wang","year":"2019","journal-title":"Eur. Rev. Med. Pharmacol. Sci."},{"key":"ref_20","first-page":"61","article-title":"Effect of alpha-lipoic acid n LPS-induced oxidative stress in the heart","volume":"60","author":"Goraca","year":"2009","journal-title":"J. Physiol. Pharmacol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"94","DOI":"10.4103\/0253-7613.125185","article-title":"Effect of aged garlic extract and S-allyl cysteine and their interaction with atenolol during isoproterenol induced myocardial toxicity in rats","volume":"46","author":"Avula","year":"2014","journal-title":"Indian. J. Pharmacol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1769","DOI":"10.12659\/MSM.914916","article-title":"The cardioprotective effects of remote ischemic conditioning in a rat model of acute myocardial infarction","volume":"25","author":"You","year":"2019","journal-title":"Med. Sci. Monit."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Ryu, J.H., and Kang, D. (2017). Physicochemical properties, biological activity, health benefits, and general limitations of aged black garlic: A review. Molecules, 22.","DOI":"10.3390\/molecules22060919"},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Jeremic, J.N., Jakovljevic, V., Zivkovic, V.I., Srejovic, I.M., Bradic, J., Milosavljevic, I.M., Mitrovic, S.J., Jovicic, N.U., Bolevich, S.B., and Svistunov, A.A. (2020). Garlic derived diallyl trisulfide in experimental metabolic syndrome: Metabolic effects and cardioprotective role. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21239100"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"955S","DOI":"10.1093\/jn\/131.3.955S","article-title":"Intake of garlic and its bioactive components","volume":"131","author":"Amagase","year":"2001","journal-title":"J. Nutr."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"3041","DOI":"10.1007\/s11064-017-2337-2","article-title":"Chronic administration of S-allylcysteine activates Nrf2 factor and enhances the activity of antioxidant enzymes in the striatum, frontal cortex and hippocampus","volume":"42","author":"Maldonado","year":"2017","journal-title":"Neurochem. Res."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"403S","DOI":"10.3945\/jn.114.208066","article-title":"Garlic-derived organic polysulfides and myocardial protection","volume":"146","author":"Bradley","year":"2016","journal-title":"J. Nutr."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1089\/ars.2008.2282","article-title":"Hydrogen sulfide increases glutathione production and suppresses oxidative stress in mitochondria","volume":"12","author":"Kimura","year":"2010","journal-title":"Antioxid. Redox. Signal."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1020S","DOI":"10.1093\/jn\/131.3.1020S","article-title":"Garlic compounds minimize intracellular oxidative stress and inhibit nuclear factor-\u03baB activation","volume":"131","author":"Ide","year":"2001","journal-title":"J. Nutr."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"170","DOI":"10.1016\/j.pupt.2017.06.006","article-title":"Effects of intranasal treatment with slow (GYY4137) and rapid (NaHS) donors of hydrogen sulfide in lipopolysaccharide-induced airway inflammation in mice","volume":"45","author":"Karaman","year":"2017","journal-title":"Pulm. Pharmacol. Ther."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"897","DOI":"10.1016\/j.pharep.2014.05.014","article-title":"Propargylglycine aggravates liver damage in LPS-treated rats: Possible relation of nitrosative stress with the inhibition of H2S formation","volume":"66","author":"Bekpinar","year":"2014","journal-title":"Pharmacol. Rep."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"71","DOI":"10.2147\/IBPC.S51434","article-title":"Potential of garlic (Allium sativum) in lowering high blood pressure: Mechanisms of action and clinical relevance","volume":"7","author":"Ried","year":"2014","journal-title":"Integr. Blood. Press. Control."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"H2410","DOI":"10.1152\/ajpheart.00044.2012","article-title":"The polysulfide diallyl trisulfide protects the ischemic myocardium by preservation of endogenous hydrogen sulfide and increasing nitric oxide bioavailability","volume":"302","author":"Predmore","year":"2012","journal-title":"Am. J. Physiol. Heart. Circ. Physiol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"777S","DOI":"10.1093\/jn\/136.3.777S","article-title":"Aged garlic extract maintains cardiovascular homeostasis in mice and rats","volume":"136","author":"Morihara","year":"2006","journal-title":"J. Nutr."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1016\/S0021-9150(98)00094-X","article-title":"Effect of allicin and ajoene, two compounds of garlic, on inducible nitric oxide synthase","volume":"139","author":"Dirsch","year":"1998","journal-title":"Atherosclerosis"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1886","DOI":"10.1161\/01.CIR.0000124231.98250.A8","article-title":"Myocyte nitric oxide synthase 2 contributes to blunted beta-adrenergic response in failing human hearts by decreasing Ca2+ transients","volume":"109","author":"Ziolo","year":"2004","journal-title":"Circulation"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"172","DOI":"10.1016\/j.cardiores.2004.06.020","article-title":"Inflammation-induced endothelial dysfunction involves reduced nitric oxide bioavailability and increased oxidant stress","volume":"64","author":"Clapp","year":"2004","journal-title":"Cardiovasc. Res."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"957","DOI":"10.2174\/1568026621666211105094939","article-title":"Garlic (Allium sativum L.): Its Chemistry, Nutritional Composition, Toxicity, and Anticancer Properties","volume":"22","author":"Rauf","year":"2022","journal-title":"Curr. Top Med. Chem."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"9","DOI":"10.2147\/IBPC.S93335","article-title":"The effect of aged garlic extract on blood pressure and other cardiovascular risk factors in uncontrolled hypertensives: The AGE at heart trial","volume":"9","author":"Ried","year":"2016","journal-title":"Integr. Blood. Press. Control"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"870","DOI":"10.1253\/circj.CJ-16-1097","article-title":"Diallyl Trisulfide Augments Ischemia-Induced Angiogenesis via an Endothelial Nitric Oxide Synthase-Dependent Mechanism","volume":"81","author":"Hayashida","year":"2017","journal-title":"Circ. J."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"12591","DOI":"10.3390\/molecules190812591","article-title":"Allicin: Chemistry and biological properties","volume":"8","author":"Borlinghaus","year":"2014","journal-title":"Molecules"},{"key":"ref_42","first-page":"1","article-title":"Garlic: A review of potential therapeutic effects","volume":"4","author":"Bayan","year":"2014","journal-title":"Avicenna. J. Phytomed."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"6494306","DOI":"10.1155\/2019\/6494306","article-title":"H2S protects against cardiac cell hypertrophy through regulation of selenoproteins","volume":"2019","author":"Greasley","year":"2019","journal-title":"Oxid. Med. Cell. Longev."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1016\/j.niox.2010.11.001","article-title":"Cardioprotective effects of hydrogen sulfide","volume":"25","author":"Veres","year":"2011","journal-title":"Nitric Oxide."},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Gruhlke, M.C.H., and Slusarenko, A.J. (2018). The chemistry of alliums. Molecules, 23.","DOI":"10.3390\/molecules23010143"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"1945","DOI":"10.1159\/000494874","article-title":"Targeting thioredoxin system with an organosulfur compound, diallyl trisulfide (DATS), attenuates progression and metastasis of triple-negative breast cancer (TNBC)","volume":"50","author":"Liu","year":"2018","journal-title":"Cell. Physiol. Biochem."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1080\/01635589409514341","article-title":"Protective effects of an aged garlic extract on doxorubicin-induced cardiotoxicity in the mouse","volume":"22","author":"Kojima","year":"1994","journal-title":"Nutr. Cancer"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"378","DOI":"10.1021\/jf010937z","article-title":"Differential effects of garlic oil and its three major organosulfur components on the hepatic detoxification system in rats","volume":"50","year":"2002","journal-title":"J. Agric. Food. Chem."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"743","DOI":"10.1016\/j.fct.2003.12.010","article-title":"The effects of allyl sulfides on the induction of phase II detoxification enzymes and liver injury by carbon tetrachloride","volume":"42","author":"Fukao","year":"2004","journal-title":"Food Chem. Toxicol."},{"key":"ref_50","unstructured":"Dehnert, H. (1988). The Isolated Perfused Warm-Blooded Heart According to Langendorff. Biological Measurement Techniques, Biomes Technik-Verlag. [1st ed.]."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/0003-2697(76)90527-3","article-title":"A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding","volume":"72","author":"Bradford","year":"1976","journal-title":"Anal. Biochem."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1006\/abio.1996.0292","article-title":"The ferric reducing ability of plasma (FRAP) as a measure of \u2018antioxidant power\u2019: The FRAP assay","volume":"239","author":"Benzie","year":"1996","journal-title":"Anal. Biochem."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"3159","DOI":"10.1038\/nprot.2006.378","article-title":"Assay for quantitative determination of glutathione and glutathione disulfide levels using enzymatic recycling method","volume":"1","author":"Rahman","year":"2006","journal-title":"Nat. Protoc."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"5392454","DOI":"10.1155\/2020\/5392454","article-title":"Oxidant\/antioxidant profile in the thoracic aneurysm of patients with the Loeys-Dietz syndrome","volume":"2020","author":"Soto","year":"2020","journal-title":"Oxid. Med. Cell. Longev."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"326","DOI":"10.1016\/j.clinbiochem.2014.09.026","article-title":"A novel and automated assay for thiol\/disulphide homeostasis","volume":"49","author":"Erel","year":"2014","journal-title":"Clin. Biochem."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"7130","DOI":"10.1016\/S0021-9258(19)42083-8","article-title":"Glutathione-S-transferases. The first enzymatic step in mercapturic acid formation","volume":"249","author":"Habig","year":"1974","journal-title":"Biol. Chem."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1016\/j.prostaglandins.2016.05.001","article-title":"Participation of oleic acid in the formation of the aortic aneurysm in Marfan syndrome patients","volume":"123","author":"Soto","year":"2016","journal-title":"Prostaglandins. Other. Lipid. Mediat."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1186\/1743-7075-8-53","article-title":"Garlic improves insulin sensitivity and associated metabolic syndromes in fructose fed rats","volume":"8","author":"Padiya","year":"2011","journal-title":"Nutr. Metab."},{"key":"ref_59","doi-asserted-by":"crossref","unstructured":"Padiya, R., Chowdhury, D., Borkar, R., Srinivas, R., Bhadra, M.P., and Banerjee, S.K. (2014). Garlic attenuates cardiac oxidative stress via activation of PI3K\/AKT\/Nrf2-Keap1 pathway in fructose-fed diabetic rat. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0094228"}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/23\/20\/12529\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T00:57:14Z","timestamp":1760144234000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/23\/20\/12529"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,10,19]]},"references-count":59,"journal-issue":{"issue":"20","published-online":{"date-parts":[[2022,10]]}},"alternative-id":["ijms232012529"],"URL":"https:\/\/doi.org\/10.3390\/ijms232012529","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,10,19]]}}}