{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T12:16:35Z","timestamp":1780056995243,"version":"3.54.0"},"reference-count":30,"publisher":"MDPI AG","issue":"20","license":[{"start":{"date-parts":[[2022,10,19]],"date-time":"2022-10-19T00:00:00Z","timestamp":1666137600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["NSFC 82160743"],"award-info":[{"award-number":["NSFC 82160743"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["Qiankehe Pingtai Rencai-GCC [2022]029-1"],"award-info":[{"award-number":["Qiankehe Pingtai Rencai-GCC [2022]029-1"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Guizhou Provincial Science and Technology Projects","award":["NSFC 82160743"],"award-info":[{"award-number":["NSFC 82160743"]}]},{"name":"Guizhou Provincial Science and Technology Projects","award":["Qiankehe Pingtai Rencai-GCC [2022]029-1"],"award-info":[{"award-number":["Qiankehe Pingtai Rencai-GCC [2022]029-1"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Aspongopus chinensis Dallas is used as a traditional Chinese medicine as well as an edible insect. Although its anti-tumor effects have been observed, the anti-tumor active component(s) in the hemolymph of A. chinensis remains unknown. In this study, a combination usage of ultrafiltration, gel filtration chromatography, FPLC and RP-HPLC to separate and purify active peptides was performed based on the proliferation of the human gastric cancer SGC-7901 cell line treated with candidates. One peptide (MW = 2853.3 Da) was isolated from the hemolymph of A. chinensis. A total of 24 amino acid residues were continuously determined for the active peptide: N\u2032-ECGYCAEKGIRCDDIHCCTGLKKK-C\u2032. In conclusion, a peptide that can inhibit the proliferation of gastric cancer SGC-7901 cells in the hemolymph of A. chinensis was purified in this study, which is homologous to members of the spider toxin protein family. These results should facilitate further works for this peptide, such as the cloning of genes, expression in vitro by prokaryotic or eukaryotic systems, more specific tests of anti-tumor activity, and so on.<\/jats:p>","DOI":"10.3390\/ijms232012535","type":"journal-article","created":{"date-parts":[[2022,10,19]],"date-time":"2022-10-19T20:32:23Z","timestamp":1666211543000},"page":"12535","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Isolation of Peptide Inhibiting SGC-7901 Cell Proliferation from Aspongopus chinensis Dallas"],"prefix":"10.3390","volume":"23","author":[{"given":"Xu-Mei","family":"Chen","sequence":"first","affiliation":[{"name":"Scientific Observing and Experimental Station of Crop Pests in Guiyang, Ministry of Agricultural and Rural Affairs, Institute of Entomology, Guizhou University, Guiyang 550025, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shu-Qi","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Life Science, Guizhou University, Guiyang 550025, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mi-Lan","family":"Cao","sequence":"additional","affiliation":[{"name":"Scientific Observing and Experimental Station of Crop Pests in Guiyang, Ministry of Agricultural and Rural Affairs, Institute of Entomology, Guizhou University, Guiyang 550025, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0523-6704","authenticated-orcid":false,"given":"Jian-Jun","family":"Guo","sequence":"additional","affiliation":[{"name":"Scientific Observing and Experimental Station of Crop Pests in Guiyang, Ministry of Agricultural and Rural Affairs, Institute of Entomology, Guizhou University, Guiyang 550025, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui","family":"Luo","sequence":"additional","affiliation":[{"name":"College of Life Science, Guizhou University, Guiyang 550025, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,10,19]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"209","DOI":"10.3322\/caac.21660","article-title":"Global cancer statistics 2020: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries","volume":"71","author":"Sung","year":"2021","journal-title":"CA A Cancer J. 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