{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T03:06:09Z","timestamp":1788404769952,"version":"build-2803163510"},"reference-count":42,"publisher":"MDPI AG","issue":"20","license":[{"start":{"date-parts":[[2022,10,19]],"date-time":"2022-10-19T00:00:00Z","timestamp":1666137600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Agencia Nacional de Investigaci\u00f3n y Desarrollo (ANID)","award":["#1200836"],"award-info":[{"award-number":["#1200836"]}]},{"name":"Agencia Nacional de Investigaci\u00f3n y Desarrollo (ANID)","award":["#1200250"],"award-info":[{"award-number":["#1200250"]}]},{"name":"Agencia Nacional de Investigaci\u00f3n y Desarrollo (ANID)","award":["#1210644"],"award-info":[{"award-number":["#1210644"]}]},{"name":"Agencia Nacional de Investigaci\u00f3n y Desarrollo (ANID)","award":["#21181617"],"award-info":[{"award-number":["#21181617"]}]},{"name":"Agencia Nacional de Investigaci\u00f3n y Desarrollo (ANID)","award":["#15130011"],"award-info":[{"award-number":["#15130011"]}]},{"name":"ANID Ph.D. student fellowship (Beca Doctorado Nacional)","award":["#1200836"],"award-info":[{"award-number":["#1200836"]}]},{"name":"ANID Ph.D. student fellowship (Beca Doctorado Nacional)","award":["#1200250"],"award-info":[{"award-number":["#1200250"]}]},{"name":"ANID Ph.D. student fellowship (Beca Doctorado Nacional)","award":["#1210644"],"award-info":[{"award-number":["#1210644"]}]},{"name":"ANID Ph.D. student fellowship (Beca Doctorado Nacional)","award":["#21181617"],"award-info":[{"award-number":["#21181617"]}]},{"name":"ANID Ph.D. student fellowship (Beca Doctorado Nacional)","award":["#15130011"],"award-info":[{"award-number":["#15130011"]}]},{"name":"CONICYT-FONDAP ACCDiS","award":["#1200836"],"award-info":[{"award-number":["#1200836"]}]},{"name":"CONICYT-FONDAP ACCDiS","award":["#1200250"],"award-info":[{"award-number":["#1200250"]}]},{"name":"CONICYT-FONDAP ACCDiS","award":["#1210644"],"award-info":[{"award-number":["#1210644"]}]},{"name":"CONICYT-FONDAP ACCDiS","award":["#21181617"],"award-info":[{"award-number":["#21181617"]}]},{"name":"CONICYT-FONDAP ACCDiS","award":["#15130011"],"award-info":[{"award-number":["#15130011"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Wound healing is a highly regulated multi-step process that involves a plethora of signals. Blood perfusion is crucial in wound healing and abnormalities in the formation of new blood vessels define the outcome of the wound healing process. Thy-1 has been implicated in angiogenesis and silencing of the Thy-1 gene retards the wound healing process. However, the role of Thy-1 in blood perfusion during wound closure remains unclear. We proposed that Thy-1 regulates vascular perfusion, affecting the healing rate in mouse skin. We analyzed the time of recovery, blood perfusion using Laser Speckle Contrast Imaging, and tissue morphology from images acquired with a Nanozoomer tissue scanner. The latter was assessed in a tissue sample taken with a biopsy punch on several days during the wound healing process. Results obtained with the Thy-1 knockout (Thy-1\u2212\/\u2212) mice were compared with control mice. Thy-1\u2212\/\u2212 mice showed at day seven, a delayed re-epithelialization, increased micro- to macro-circulation ratio, and lower blood perfusion in the wound area. In addition, skin morphology displayed a flatter epidermis, fewer ridges, and almost no stratum granulosum or corneum, while the dermis was thicker, showing more fibroblasts and fewer lymphocytes. Our results suggest a critical role for Thy-1 in wound healing, particularly in vascular dynamics.<\/jats:p>","DOI":"10.3390\/ijms232012539","type":"journal-article","created":{"date-parts":[[2022,10,19]],"date-time":"2022-10-19T20:32:23Z","timestamp":1666211543000},"page":"12539","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["The GPI-Anchored Protein Thy-1\/CD90 Promotes Wound Healing upon Injury to the Skin by Enhancing Skin Perfusion"],"prefix":"10.3390","volume":"23","author":[{"given":"Leonardo A.","family":"P\u00e9rez","sequence":"first","affiliation":[{"name":"Laboratory of Cellular Communication, Center for Studies on Exercise, Metabolism and Cancer (CEMC), Faculty of Medicine, University of Chile, Santiago 8380453, Chile"},{"name":"Advanced Center for Chronic Diseases (ACCDiS), Faculty of Medicine, Universidad de Chile, Santiago 8380453, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9","family":"Le\u00f3n","sequence":"additional","affiliation":[{"name":"Vascular Physiology Laboratory, Department of Basic Sciences, Faculty of Sciences, Universidad del B\u00edo-B\u00edo, Chill\u00e1n 3800708, Chile"},{"name":"Escuela de Enfermer\u00eda, Facultad de Salud, Universidad Santo Tom\u00e1s, Los \u00c1ngeles 3460000, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan","family":"L\u00f3pez","sequence":"additional","affiliation":[{"name":"Vascular Physiology Laboratory, Department of Basic Sciences, Faculty of Sciences, Universidad del B\u00edo-B\u00edo, Chill\u00e1n 3800708, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0945-571X","authenticated-orcid":false,"given":"Daniela","family":"Rojas","sequence":"additional","affiliation":[{"name":"Departamento de Patolog\u00eda Cl\u00ednica, Facultad de Ciencias Veterinarias, Universidad de Concepci\u00f3n, Chill\u00e1n 4030000, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Montserrat","family":"Reyes","sequence":"additional","affiliation":[{"name":"Department of Pathology and Oral Medicine, Facultad de Odontolog\u00eda, Universidad de Chile, Santiago 8380544, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pamela","family":"Contreras","sequence":"additional","affiliation":[{"name":"Laboratory of Cellular Communication, Center for Studies on Exercise, Metabolism and Cancer (CEMC), Faculty of Medicine, University of Chile, Santiago 8380453, Chile"},{"name":"Advanced Center for Chronic Diseases (ACCDiS), Faculty of Medicine, Universidad de Chile, Santiago 8380453, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8755-1590","authenticated-orcid":false,"given":"Andrew F. G.","family":"Quest","sequence":"additional","affiliation":[{"name":"Laboratory of Cellular Communication, Center for Studies on Exercise, Metabolism and Cancer (CEMC), Faculty of Medicine, University of Chile, Santiago 8380453, Chile"},{"name":"Advanced Center for Chronic Diseases (ACCDiS), Faculty of Medicine, Universidad de Chile, Santiago 8380453, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7688-4621","authenticated-orcid":false,"given":"Carlos","family":"Escudero","sequence":"additional","affiliation":[{"name":"Vascular Physiology Laboratory, Department of Basic Sciences, Faculty of Sciences, Universidad del B\u00edo-B\u00edo, Chill\u00e1n 3800708, Chile"},{"name":"Group of Research and Innovation in Vascular Health (GRIVAS Health), Chill\u00e1n 3800708, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9690-9638","authenticated-orcid":false,"given":"Lisette","family":"Leyton","sequence":"additional","affiliation":[{"name":"Laboratory of Cellular Communication, Center for Studies on Exercise, Metabolism and Cancer (CEMC), Faculty of Medicine, University of Chile, Santiago 8380453, Chile"},{"name":"Advanced Center for Chronic Diseases (ACCDiS), Faculty of Medicine, Universidad de Chile, Santiago 8380453, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,10,19]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Schilrreff, P., and Alexiev, U. 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