{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T18:28:22Z","timestamp":1785522502127,"version":"3.56.0"},"reference-count":86,"publisher":"MDPI AG","issue":"20","license":[{"start":{"date-parts":[[2022,10,19]],"date-time":"2022-10-19T00:00:00Z","timestamp":1666137600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Ministry of Education"},{"name":"Science in Poland within statutory activity of Medical University of Lublin"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Aeromonas species are opportunistic bacteria causing a vast spectrum of human diseases, including skin and soft tissue infections, meningitis, endocarditis, peritonitis, gastroenteritis, and finally hemorrhagic septicemia. The aim of our research was to indicate the molecular alterations in proteins and lipids profiles resulting from Aeromonas sobria and A. salmonicida subsp. salmonicida infection in trout kidney tissue samples. We successfully applied FT-IR (Fourier transform infrared) spectroscopy and MALDI-MSI (matrix-assisted laser desorption\/ionization mass spectrometry imaging) to monitor changes in the structure and compositions of lipids, secondary conformation of proteins, and provide useful information concerning disease progression. Our findings indicate that the following spectral bands\u2019 absorbance ratios (spectral biomarkers) can be used to discriminate healthy tissue from pathologically altered tissue, for example, lipids (CH2\/CH3), amide I\/amide II, amide I\/CH2 and amide I\/CH3. Spectral data obtained from 10 single measurements of each specimen indicate numerous abnormalities concerning proteins, lipids, and phospholipids induced by Aeromonas infection, suggesting significant disruption of the cell membranes. Moreover, the increase in the content of lysolipids such as lysophosphosphatidylcholine was observed. The results of this study suggest the application of both methods MALDI-MSI and FT-IR as accurate methods for profiling biomolecules and identifying biochemical changes in kidney tissue during the progression of Aeromonas infection.<\/jats:p>","DOI":"10.3390\/ijms232012551","type":"journal-article","created":{"date-parts":[[2022,10,19]],"date-time":"2022-10-19T20:32:23Z","timestamp":1666211543000},"page":"12551","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Evaluation of Proteomic and Lipidomic Changes in Aeromonas-Infected Trout Kidney Tissue with the Use of FT-IR Spectroscopy and MALDI Mass Spectrometry Imaging"],"prefix":"10.3390","volume":"23","author":[{"given":"Joanna","family":"Matys","sequence":"first","affiliation":[{"name":"Department of Biopharmacy, Medical University of Lublin, Chod\u017aki 4a, 20-093 Lublin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2975-9593","authenticated-orcid":false,"given":"Anna","family":"Turska-Szewczuk","sequence":"additional","affiliation":[{"name":"Department of Genetics and Microbiology, Institute of Biological Sciences, Maria Curie-Sk\u0142odowska University, Akademicka 19, 20-033 Lublin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8549-9848","authenticated-orcid":false,"given":"Barbara","family":"Gieroba","sequence":"additional","affiliation":[{"name":"Independent Unit of Spectroscopy and Chemical Imaging, Medical University of Lublin, Chod\u017aki 4a, 20-093 Lublin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Kurzylewska","sequence":"additional","affiliation":[{"name":"Department of Genetics and Microbiology, Institute of Biological Sciences, Maria Curie-Sk\u0142odowska University, Akademicka 19, 20-033 Lublin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5515-8329","authenticated-orcid":false,"given":"Agnieszka","family":"P\u0119kala-Safi\u0144ska","sequence":"additional","affiliation":[{"name":"Department of Preclinical Sciences and Infectious Diseases, Poznan University of Life Sciences, Wo\u0142y\u0144ska 35, 60-637 Pozna\u0144, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0739-3567","authenticated-orcid":false,"given":"Anna","family":"Sroka-Bartnicka","sequence":"additional","affiliation":[{"name":"Department of Genetics and Microbiology, Institute of Biological Sciences, Maria Curie-Sk\u0142odowska University, Akademicka 19, 20-033 Lublin, Poland"},{"name":"Independent Unit of Spectroscopy and Chemical Imaging, Medical University of Lublin, Chod\u017aki 4a, 20-093 Lublin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,10,19]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1549","DOI":"10.1099\/jmm.0.014316-0","article-title":"Pathogenic potential of Aeromonas hydrophila isolated from surface waters in Kolkata, India","volume":"58","author":"Bhowmik","year":"2009","journal-title":"J. Med. Microbiol."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Austin, B., and Austin, D.A. (2016). Bacterial Fish Pathogens: Disease of Farmed and Wild Fish, Springer. [6th ed.]. Imprint: pp. 1 online resource (XXXV, 198\u2013243).","DOI":"10.1007\/978-3-319-32674-0"},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Kwon, J., Kim, S.G., Kim, S.W., Yun, S., Kim, H.J., Giri, S.S., Han, S.J., Oh, W.T., and Park, S.C. (2020). A Case of Mortality Caused by Aeromonas hydrophila in Wild-Caught Red-Eyed Crocodile Skinks (Tribolonotus gracilis). Vet. Sci., 7.","DOI":"10.3390\/vetsci7010004"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1007\/BF02601673","article-title":"Pathogenicity of Aeromonas-Hydrophila in Red Leg Disease in Frogs","volume":"1","author":"Rigney","year":"1978","journal-title":"Curr. Microbiol."},{"key":"ref_5","unstructured":"Wright, K.M., and Whitaker, B.R. (2001). Amphibian Medicine and Captive Husbandry, Krieger Pub. Co.. [Original ed.]."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1678","DOI":"10.1128\/aem.60.5.1678-1680.1994","article-title":"An Outbreak of Aeromonas-Hydrophila Infection in Turtles (Pseudemis-Scripta)","volume":"60","author":"Pasquale","year":"1994","journal-title":"Appl. Environ. Microb."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"625023","DOI":"10.1100\/2012\/625023","article-title":"Emerging Aeromonas species infections and their significance in public health","volume":"2012","author":"Igbinosa","year":"2012","journal-title":"Sci. World J."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1128\/CMR.00039-09","article-title":"The genus Aeromonas: Taxonomy, pathogenicity, and infection","volume":"23","author":"Janda","year":"2010","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"El-Gohary, F.A., Zahran, E., El-Gawad, E.A.A., El-Gohary, A.H., Abdelhamid, F.M., El-Mleeh, A., Elmahallawy, E.K., and Elsayed, M.M. (2020). Investigation of the Prevalence, Virulence Genes, and Antibiogram of Motile Aeromonads Isolated from Nile Tilapia Fish Farms in Egypt and Assessment of their Water Quality. Animals, 10.","DOI":"10.3390\/ani10081432"},{"key":"ref_10","first-page":"163040","article-title":"Aeromonas hydrophila Sepsis Associated with Consumption of Raw Oysters","volume":"2014","author":"Nikiforov","year":"2014","journal-title":"Case Rep. Infect. Dis."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"261","DOI":"10.2478\/jvetres-2018-0037","article-title":"Contemporary Threats of Bacterial Infections in Freshwater Fish","volume":"62","year":"2018","journal-title":"J. Vet. Res."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.vetmic.2013.06.025","article-title":"Virulence, genomic features, and plasticity of Aeromonas salmonicida subsp. salmonicida, the causative agent of fish furunculosis","volume":"169","author":"Tanaka","year":"2014","journal-title":"Vet. Microbiol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1111\/j.1574-695X.2002.tb00559.x","article-title":"Incidence of toxic Aeromonas isolated from food and human infection","volume":"32","author":"Martins","year":"2002","journal-title":"FEMS Immunol. Med. Microbiol."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Fernandez-Bravo, A., and Figueras, M.J. (2020). An Update on the Genus Aeromonas: Taxonomy, Epidemiology, and Pathogenicity. Microorganisms, 8.","DOI":"10.3390\/microorganisms8010129"},{"key":"ref_15","first-page":"250","article-title":"Histopathological findings in farmed rainbow trout (Oncorhynchus mykiss) naturally infected with 3 different Aeromonas species","volume":"79","year":"2015","journal-title":"Can. J. Vet. Res."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1186\/1752-1947-8-315","article-title":"Aeromonas sobria necrotizing fasciitis and sepsis in an immunocompromised patient: A case report and review of the literature","volume":"8","author":"Spadaro","year":"2014","journal-title":"J. Med. Case Rep."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"2313","DOI":"10.1128\/AEM.02535-09","article-title":"Distribution of virulence factors and molecular fingerprinting of Aeromonas species isolates from water and clinical samples: Suggestive evidence of water-to-human transmission","volume":"76","author":"Khajanchi","year":"2010","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1298","DOI":"10.3390\/md12031298","article-title":"Structural studies of the lipopolysaccharide from the fish pathogen Aeromonas veronii strain Bs19, serotype O16","volume":"12","author":"Duda","year":"2014","journal-title":"Mar. Drugs"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1706","DOI":"10.4315\/0362-028X.JFP-18-545","article-title":"Molecular Identification and Virulence Potential of the Genus Aeromonas Isolated from Wild Rainbow Trout (Oncorhynchus mykiss) in Mexico","volume":"82","year":"2019","journal-title":"J. Food Prot."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"70","DOI":"10.3389\/fcimb.2013.00070","article-title":"Modulation of host immune defenses by Aeromonas and Yersinia species: Convergence on toxins secreted by various secretion systems","volume":"3","author":"Rosenzweig","year":"2013","journal-title":"Front. Cell. Infect. Microbiol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"137","DOI":"10.21307\/pjm-2018-020","article-title":"Bacteriological, Clinical and Virulence Aspects of Aeromonas-associated Diseases in Humans","volume":"67","author":"Bhowmick","year":"2018","journal-title":"Pol. J. Microbiol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1128\/MMBR.00052-12","article-title":"Role of pore-forming toxins in bacterial infectious diseases","volume":"77","author":"Los","year":"2013","journal-title":"Microbiol. Mol. Biol. Rev."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1016\/S0168-1605(02)00393-8","article-title":"Characterisation of Aeromonas spp. isolated from frozen fish intended for human consumption in Mexico","volume":"84","author":"Figueras","year":"2003","journal-title":"Int. J. Food Microbiol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"2808","DOI":"10.1128\/IAI.68.5.2808-2818.2000","article-title":"The cytotoxic enterotoxin of Aeromonas hydrophila induces proinflammatory cytokine production and activates arachidonic acid metabolism in macrophages","volume":"68","author":"Chopra","year":"2000","journal-title":"Infect. Immun."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.plipres.2009.07.003","article-title":"Lipids in host-pathogen interactions: Pathogens exploit the complexity of the host cell lipidome","volume":"49","author":"Batenburg","year":"2010","journal-title":"Prog. Lipid Res."},{"key":"ref_26","first-page":"1522","article-title":"Active Shiga-Like Toxin Produced by Some Aeromonas spp., Isolated in Mexico City","volume":"7","year":"2016","journal-title":"Front. Microbiol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1563","DOI":"10.1111\/j.1469-0691.2010.03203.x","article-title":"Human isolates of Aeromonas possess Shiga toxin genes (stx1 and stx2) highly similar to the most virulent gene variants of Escherichia coli","volume":"16","author":"Alperi","year":"2010","journal-title":"Clin. Microbiol. Infect."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"2359","DOI":"10.3390\/toxins2102359","article-title":"Diversity and impact of prokaryotic toxins on aquatic environments: A review","volume":"2","author":"Valerio","year":"2010","journal-title":"Toxins"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1585","DOI":"10.3389\/fmicb.2018.01585","article-title":"Whole Cell Cross-Linking to Discover Host-Microbe Protein Cognate Receptor\/Ligand Pairs","volume":"9","author":"Weimer","year":"2018","journal-title":"Front. Microbiol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"855","DOI":"10.1002\/jbio.201300131","article-title":"Molecular pathology via IR and Raman spectral imaging","volume":"6","author":"Diem","year":"2013","journal-title":"J. Biophotonics"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/j.aca.2020.03.037","article-title":"FTIR-assisted MALDI-TOF MS for the identification and typing of bacteria","volume":"1111","author":"Feng","year":"2020","journal-title":"Anal. Chim. Acta"},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Chalmers, J.M., and Griffiths, P.R. (2002). Handbook of Vibrational Spectroscopy, J. Wiley.","DOI":"10.1002\/0470027320"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"653","DOI":"10.2147\/CMAR.S240285","article-title":"Fourier Transform Infrared Spectroscopy Monitoring of Dihydroartemisinin-Induced Growth Inhibition in Ovarian Cancer Cells and Normal Ovarian Surface Epithelial Cells","volume":"12","author":"Li","year":"2020","journal-title":"Cancer Manag. Res."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"459","DOI":"10.1016\/j.bbamem.2016.12.005","article-title":"Characterization of extracellular vesicles by IR spectroscopy: Fast and simple classification based on amide and CH stretching vibrations","volume":"1859","author":"Mihaly","year":"2017","journal-title":"Biochim. Biophys. Acta Biomembr."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"7178","DOI":"10.1021\/acs.energyfuels.5b02064","article-title":"Comprehensive Investigation of Various Structural Features of Bituminous Coals Using Advanced Analytical Techniques","volume":"29","author":"Li","year":"2015","journal-title":"Energ. Fuel"},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Ricciardi, V., Portaccio, M., Manti, L., and Lepore, M. (2020). An FTIR Microspectroscopy Ratiometric Approach for Monitoring X-ray Irradiation Effects on SH-SY5Y Human Neuroblastoma Cells. Appl. Sci., 10.","DOI":"10.3390\/app10082974"},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Munajad, A., Subroto, C. (2018). Fourier Transform Infrared (FTIR) Spectroscopy Analysis of Transformer Paper in Mineral Oil-Paper Composite Insulation under Accelerated Thermal Aging. Energies, 11.","DOI":"10.3390\/en11020364"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"549","DOI":"10.1111\/j.1745-7270.2007.00320.x","article-title":"Fourier transform infrared spectroscopic analysis of protein secondary structures","volume":"39","author":"Kong","year":"2007","journal-title":"Acta Biochim. Biophys. Sin."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1354","DOI":"10.1021\/la047735s","article-title":"L-glutathione chemisorption on gold and acid\/base induced structural changes: A PM-IRRAS and time-resolved in situ ATR-IR spectroscopic study","volume":"21","author":"Bieri","year":"2005","journal-title":"Langmuir"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"4200","DOI":"10.5504\/BBEQ.2013.0106","article-title":"Application of Fourier Transform Infrared Spectroscopy for Tumor Diagnosis","volume":"27","author":"Simonova","year":"2013","journal-title":"Biotechnol. Biotechnol. Equip."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"396","DOI":"10.1504\/IJNBM.2009.027736","article-title":"FT-IR spectroscopy as a tool for identification of apoptosis-induced structural changes in A549 cells treated with PM 701","volume":"2","author":"Ahmed","year":"2009","journal-title":"Int. J. Nano Biomater."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1968","DOI":"10.1046\/j.1432-1033.2002.02841.x","article-title":"Infrared spectroscopy as a tool for discrimination between sensitive and multiresistant K562 cells","volume":"269","author":"Gaigneaux","year":"2002","journal-title":"Eur. J. Biochem."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1117\/12.450167","article-title":"Application of FTIR microspectroscopy for the follow-up of childhood leukemia chemotherapy","volume":"4491","author":"Mordechai","year":"2001","journal-title":"Proc. Spie"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1042\/bj20021021","article-title":"Monitoring of apoptosis of HL60 cells by Fourier-transform infrared spectroscopy","volume":"369","author":"Gasparri","year":"2003","journal-title":"Biochem. J."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"1451","DOI":"10.1016\/j.bbamem.2013.10.019","article-title":"The Fluid-Mosaic Model of Membrane Structure: Still relevant to understanding the structure, function and dynamics of biological membranes after more than 40 years","volume":"1838","author":"Nicolson","year":"2014","journal-title":"Biochim. Biophys. Acta"},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Ali, M.H.M., Rakib, F., Nischwitz, V., Ullah, E., Mall, R., Shraim, A.M., Ahmad, M.I., Ghouri, Z.K., McNaughton, D., and Kuppers, S. (2018). Application of FTIR and LA-ICPMS Spectroscopies as a Possible Approach for Biochemical Analyses of Different Rat Brain Regions. Appl. Sci., 8.","DOI":"10.3390\/app8122436"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"2585","DOI":"10.3892\/mmr.2014.3088","article-title":"Fourier transform infrared microspectroscopy monitoring of 5-fluorouracil-induced apoptosis in SW620 colon cancer cells","volume":"11","author":"Gao","year":"2015","journal-title":"Mol. Med. Rep."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1186\/s13048-018-0434-8","article-title":"Characterization of ovarian cancer cells an tissues by Fourier transform infrared spectroscopy","volume":"11","author":"Li","year":"2018","journal-title":"J. Ovarian Res."},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Bik, E., Ishigaki, M., Blat, A., Jasztal, A., Ozaki, Y., Malek, K., and Baranska, M. (2020). Lipid Droplet Composition Varies Based on Medaka Fish Eggs Development as Revealed by NIR-, MIR-, and Raman Imaging. Molecules, 25.","DOI":"10.3390\/molecules25040817"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"3150","DOI":"10.1111\/jcmm.13273","article-title":"Hepatitis C virus and proprotein convertase subtilisin\/kexin type 9: A detrimental interaction to increase viral infectivity and disrupt lipid metabolism","volume":"21","author":"Pirro","year":"2017","journal-title":"J. Cell. Mol. Med."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"1216","DOI":"10.1073\/pnas.1310524111","article-title":"Chemo-informatic strategy for imaging mass spectrometry-based hyperspectral profiling of lipid signatures in colorectal cancer","volume":"111","author":"Veselkov","year":"2014","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"981","DOI":"10.1074\/mcp.M700382-MCP200","article-title":"Analysis of the zebrafish proteome during embryonic development","volume":"7","author":"Lucitt","year":"2008","journal-title":"Mol. Cell. Proteom."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"6969","DOI":"10.1007\/s00216-019-02069-6","article-title":"Fourier transform infrared spectroscopic imaging of colon tissues: Evaluating the significance of amide I and C-H stretching bands in diagnostic applications with machine learning","volume":"411","author":"Song","year":"2019","journal-title":"Anal. Bioanal. Chem."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"2954","DOI":"10.1039\/C8AN01929K","article-title":"Three-dimensional depth profiling of prostate tissue by micro ATR-FTIR spectroscopic imaging with variable angles of incidence","volume":"144","author":"Song","year":"2019","journal-title":"Analyst"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"119","DOI":"10.7171\/jbt.13-2403-002","article-title":"Optimization of whole-body zebrafish sectioning methods for mass spectrometry imaging","volume":"24","author":"Nelson","year":"2013","journal-title":"J. Biomol. Tech."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"697","DOI":"10.1016\/j.neuron.2014.10.011","article-title":"Direct Targeted Quantitative Molecular Imaging of Neurotransmitters in Brain Tissue Sections","volume":"84","author":"Shariatgorji","year":"2014","journal-title":"Neuron"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"488","DOI":"10.1111\/jnc.14559","article-title":"Molecular imaging mass spectrometry for probing protein dynamics in neurodegenerative disease pathology","volume":"151","author":"Michno","year":"2019","journal-title":"J. Neurochem."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"4848","DOI":"10.1128\/IAI.72.8.4848-4858.2004","article-title":"Aeromonas hydrophila beta-hemolysin induces active chloride secretion in colon epithelial cells (HT-29\/B6)","volume":"72","author":"Epple","year":"2004","journal-title":"Infect. Immun."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"925","DOI":"10.1111\/1462-2920.12288","article-title":"Bacterial pathogens, virulence mechanism and host defence in marine macroalgae","volume":"16","author":"Egan","year":"2014","journal-title":"Environ. Microbiol."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"1237","DOI":"10.3389\/fimmu.2019.01237","article-title":"Aeromonas salmonicida subsp. salmonicida Early Infection and Immune Response of Atlantic Cod (Gadus morhua L.) Primary Macrophages","volume":"10","author":"Hossain","year":"2019","journal-title":"Front. Immunol."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1186\/s13024-018-0252-x","article-title":"Vibrational spectroscopy: A promising approach to discriminate neurodegenerative disorders","volume":"13","author":"Paraskevaidi","year":"2018","journal-title":"Mol. Neurodegener."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"744","DOI":"10.1016\/j.pharep.2015.05.001","article-title":"Vascular diseases investigated ex vivo by using Raman, FT-IR and complementary methods","volume":"67","author":"Marzec","year":"2015","journal-title":"Pharmacol. Rep."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"1897","DOI":"10.3748\/wjg.v9.i9.1897","article-title":"FT-IR spectroscopic analysis of normal and cancerous tissues of esophagus","volume":"9","author":"Wang","year":"2003","journal-title":"World J. Gastroenterol."},{"key":"ref_64","doi-asserted-by":"crossref","unstructured":"Sreedhar, H., Varma, V.K., Nguyen, P.L., Davidson, B., Akkina, S., Guzman, G., Setty, S., Kajdacsy-Balla, A., and Walsh, M.J. (2015). High-definition Fourier Transform Infrared (FT-IR) spectroscopic imaging of human tissue sections towards improving pathology. J. Vis. Exp., 52332.","DOI":"10.3791\/52332-v"},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1186\/1475-2859-13-86","article-title":"Fourier transform infrared spectroscopy for the prediction of fatty acid profiles in Mucor fungi grown in media with different carbon sources","volume":"13","author":"Shapaval","year":"2014","journal-title":"Microb. Cell. Fact."},{"key":"ref_66","doi-asserted-by":"crossref","unstructured":"Balan, V., Mihai, C.T., Cojocaru, F.D., Uritu, C.M., Dodi, G., Botezat, D., and Gardikiotis, I. (2019). Vibrational Spectroscopy Fingerprinting in Medicine: From Molecular to Clinical Practice. Materials, 12.","DOI":"10.3390\/ma12182884"},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"e147418","DOI":"10.1590\/1678-9199-jvatitd-1474-18","article-title":"Identification of a pore-forming protein from sea anemone Anthopleura dowii Verrill (1869) venom by mass spectrometry","volume":"25","year":"2019","journal-title":"J. Venom. Anim. Toxins"},{"key":"ref_68","doi-asserted-by":"crossref","unstructured":"Iacovache, I., Degiacomi, M.T., Pernot, L., Ho, S., Schiltz, M., Dal Peraro, M., and van der Goot, F.G. (2011). Dual chaperone role of the C-terminal propeptide in folding and oligomerization of the pore-forming toxin aerolysin. PLoS Pathog., 7.","DOI":"10.1371\/journal.ppat.1002135"},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"235","DOI":"10.15252\/embr.201540851","article-title":"Structural basis for receptor recognition and pore formation of a zebrafish aerolysin-like protein","volume":"17","author":"Jia","year":"2016","journal-title":"EMBO Rep."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"457","DOI":"10.1038\/sj.emboj.7600959","article-title":"A rivet model for channel formation by aerolysin-like pore-forming toxins","volume":"25","author":"Iacovache","year":"2006","journal-title":"EMBO J."},{"key":"ref_71","doi-asserted-by":"crossref","unstructured":"Masuyer, G. (2020). Crystal Structure of Exotoxin A from Aeromonas Pathogenic Species. Toxins, 12.","DOI":"10.3390\/toxins12060397"},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"1521","DOI":"10.1016\/j.chembiol.2015.09.015","article-title":"Host-Microbe Protein Interactions during Bacterial Infection","volume":"22","author":"Schweppe","year":"2015","journal-title":"Chem. Biol."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"334","DOI":"10.1016\/j.fsi.2016.12.006","article-title":"Aeromonas salmonicida type III secretion system-effectors-mediated immune suppression in rainbow trout (Oncorhynchus mykiss)","volume":"60","author":"Origgi","year":"2017","journal-title":"Fish Shellfish Immunol."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"381","DOI":"10.1111\/1751-7915.12091","article-title":"Aeromonas salmonicida subsp. salmonicida in the light of its type-three secretion system","volume":"7","author":"Frey","year":"2014","journal-title":"Microb. Biotechnol."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"1285","DOI":"10.1128\/JCM.42.3.1285-1287.2004","article-title":"Type III secretion system genes in clinical Aeromonas isolates","volume":"42","author":"Chacon","year":"2004","journal-title":"J. Clin. Microbiol."},{"key":"ref_76","doi-asserted-by":"crossref","unstructured":"Rangel, L.T., Marden, J., Colston, S., Setubal, J.C., Graf, J., and Gogarten, J.P. (2019). Identification and characterization of putative Aeromonas spp. T3SS effectors. PLoS ONE, 14.","DOI":"10.1101\/570887"},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"4087","DOI":"10.1074\/jbc.M109.071035","article-title":"Sequestering of Rac by the Yersinia effector YopO blocks Fcgamma receptor-mediated phagocytosis","volume":"285","author":"Groves","year":"2010","journal-title":"J. Biol. Chem."},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"1836","DOI":"10.3389\/fimmu.2017.01836","article-title":"Macrophage-Bacteria Interactions-A Lipid-Centric Relationship","volume":"8","author":"Teng","year":"2017","journal-title":"Front. Immunol."},{"key":"ref_79","doi-asserted-by":"crossref","unstructured":"Casares, D., Escriba, P.V., and Rossello, C.A. (2019). Membrane Lipid Composition: Effect on Membrane and Organelle Structure, Function and Compartmentalization and Therapeutic Avenues. Int. J. Mol. Sci., 20.","DOI":"10.3390\/ijms20092167"},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1038\/nrm.2017.138","article-title":"Understanding the diversity of membrane lipid composition","volume":"19","author":"Harayama","year":"2018","journal-title":"Nat. Rev. Mol. Cell. Biol."},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.fgb.2012.11.008","article-title":"Combining light microscopy, dielectric spectroscopy, MALDI intact cell mass spectrometry, FTIR spectromicroscopy and multivariate data mining for morphological and physiological bioprocess characterization of filamentous organisms","volume":"51","author":"Posch","year":"2013","journal-title":"Fungal Genet. Biol."},{"key":"ref_82","doi-asserted-by":"crossref","unstructured":"Su, K.Y., and Lee, W.L. (2020). Fourier Transform Infrared Spectroscopy as a Cancer Screening and Diagnostic Tool: A Review and Prospects. Cancers, 12.","DOI":"10.3390\/cancers12010115"},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"6561","DOI":"10.1128\/JB.187.18.6561-6564.2005","article-title":"Evolutionary and diagnostic implications of intragenomic heterogeneity in the 16S rRNA gene in Aeromonas strains","volume":"187","author":"Morandi","year":"2005","journal-title":"J. Bacteriol."},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"550","DOI":"10.3349\/ymj.2015.56.2.550","article-title":"Comparison of MALDI-TOF MS, Housekeeping Gene Sequencing, and 16S rRNA Gene Sequencing for Identification of Aeromonas Clinical Isolates","volume":"56","author":"Shin","year":"2015","journal-title":"Yonsei Med. J."},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"1671","DOI":"10.1128\/jcm.35.7.1671-1674.1997","article-title":"Identification of Aeromonas clinical isolates by restriction fragment length polymorphism of PCR-amplified 16S rRNA genes","volume":"35","author":"Borrell","year":"1997","journal-title":"J. Clin. Microbiol."},{"key":"ref_86","doi-asserted-by":"crossref","first-page":"840","DOI":"10.1194\/jlr.D013060","article-title":"A simple desalting method for direct MALDI mass spectrometry profiling of tissue lipids","volume":"52","author":"Wang","year":"2011","journal-title":"J. Lipid Res."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/23\/20\/12551\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T00:57:42Z","timestamp":1760144262000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/23\/20\/12551"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,10,19]]},"references-count":86,"journal-issue":{"issue":"20","published-online":{"date-parts":[[2022,10]]}},"alternative-id":["ijms232012551"],"URL":"https:\/\/doi.org\/10.3390\/ijms232012551","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,10,19]]}}}