{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T16:38:31Z","timestamp":1791218311622,"version":"4.1.0"},"reference-count":53,"publisher":"MDPI AG","issue":"20","license":[{"start":{"date-parts":[[2022,10,20]],"date-time":"2022-10-20T00:00:00Z","timestamp":1666224000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Narodowe Centrum Nauki","award":["2021\/41\/N\/ST4\/02164"],"award-info":[{"award-number":["2021\/41\/N\/ST4\/02164"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>The accurate identification of microorganisms belonging to vaginal microflora is crucial for establishing which microorganisms are responsible for microbial shifting from beneficial symbiotic to pathogenic bacteria and understanding pathogenesis leading to vaginosis and vaginal infections. In this study, we involved the surface-enhanced Raman spectroscopy (SERS) technique to compile the spectral signatures of the most significant microorganisms being part of the natural vaginal microbiota and some vaginal pathogens. Obtained data will supply our still developing spectral SERS database of microorganisms. The SERS results were assisted by Partial Least Squares Regression (PLSR), which visually discloses some dependencies between spectral images and hence their biochemical compositions of the outer structure. In our work, we focused on the most common and typical of the reproductive system microorganisms (Lactobacillus spp. and Bifidobacterium spp.) and vaginal pathogens: bacteria (e.g., Gardnerella vaginalis, Prevotella bivia, Atopobium vaginae), fungi (e.g., Candida albicans, Candida glabrata), and protozoa (Trichomonas vaginalis). The obtained results proved that each microorganism has its unique spectral fingerprint that differentiates it from the rest. Moreover, the discrimination was obtained at a high level of explained information by subsequent factors, e.g., in the inter-species distinction of Candida spp. the first three factors explain 98% of the variance in block Y with 95% of data within the X matrix, while in differentiation between Lactobacillus spp. and Bifidobacterium spp. (natural flora) and pathogen (e.g., Candida glabrata) the information is explained at the level of 45% of the Y matrix with 94% of original data. PLSR gave us insight into discriminating variables based on which the marker bands representing specific compounds in the outer structure of microorganisms were found: for Lactobacillus spp. 1400 cm\u22121, for fungi 905 and 1209 cm\u22121, and for protozoa 805, 890, 1062, 1185, 1300, 1555, and 1610 cm\u22121. Then, they can be used as significant marker bands in the analysis of clinical subjects, e.g., vaginal swabs.<\/jats:p>","DOI":"10.3390\/ijms232012576","type":"journal-article","created":{"date-parts":[[2022,10,20]],"date-time":"2022-10-20T22:49:46Z","timestamp":1666306186000},"page":"12576","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["SERS-PLSR Analysis of Vaginal Microflora: Towards the Spectral Library of Microorganisms"],"prefix":"10.3390","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1321-3884","authenticated-orcid":false,"given":"Sylwia Magdalena","family":"Berus","sequence":"first","affiliation":[{"name":"Institute of Physical Chemistry, Polish Academy of Sciences, Kasprzaka 44\/52, 01-224 Warsaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5885-9550","authenticated-orcid":false,"given":"Monika","family":"Adamczyk-Pop\u0142awska","sequence":"additional","affiliation":[{"name":"Department of Molecular Virology, Faculty of Biology, University of Warsaw, Miecznikowa 1, 02-096 Warsaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7051-9524","authenticated-orcid":false,"given":"Katarzyna","family":"Go\u017adzik","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Faculty of Biology, University of Warsaw, Miecznikowa 1, 02-096 Warsaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gra\u017cyna","family":"Przedpe\u0142ska","sequence":"additional","affiliation":[{"name":"Department of Dermatology and Venerology, Infant Jesus Clinical Hospital, Koszykowa 82a, 02-008 Warsaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7308-0609","authenticated-orcid":false,"given":"Tomasz R.","family":"Szymborski","sequence":"additional","affiliation":[{"name":"Institute of Physical Chemistry, Polish Academy of Sciences, Kasprzaka 44\/52, 01-224 Warsaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9919-4447","authenticated-orcid":false,"given":"Yuriy","family":"Stepanenko","sequence":"additional","affiliation":[{"name":"Institute of Physical Chemistry, Polish Academy of Sciences, Kasprzaka 44\/52, 01-224 Warsaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Agnieszka","family":"Kami\u0144ska","sequence":"additional","affiliation":[{"name":"Institute of Physical Chemistry, Polish Academy of Sciences, Kasprzaka 44\/52, 01-224 Warsaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,10,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"856","DOI":"10.1093\/clinids\/12.5.856","article-title":"Emerging Role of Lactobacilli in the Control and Maintenance of the Vaginal Bacterial Microflora","volume":"12","author":"Cook","year":"1990","journal-title":"Rev. Infect. Dis."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"S273","DOI":"10.1093\/clinids\/16.Supplement_4.S273","article-title":"The Normal Vaginal Flora, H2O2-Producing Lactobacilli, and Bacterial Vaginosis in Pregnant Women","volume":"16","author":"Hillier","year":"1993","journal-title":"Clin. Infect. Dis."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Verstraelen, H., Verhelst, R., Claeys, G., De Backer, E., Temmerman, M., and Vaneechoutte, M. (2009). Longitudinal Analysis of the Vaginal Microflora in Pregnancy Suggests That L. Crispatus Promotes the Stability of the Normal Vaginal Microflora and That L. Gasseri and\/or L. Iners Are More Conducive to the Occurrence of Abnormal Vaginal Microflora. BMC Microbiol., 9.","DOI":"10.1186\/1471-2180-9-116"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1058","DOI":"10.1093\/infdis\/174.5.1058","article-title":"Hydrogen Peroxide\u2014Producing Lactobacilli and Acquisition of Vaginal Infections","volume":"174","author":"Hawes","year":"1996","journal-title":"J. Infect. Dis."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Verhelst, R., Verstraelen, H., Claeys, G., Verschraegen, G., Van Simaey, L., De Ganck, C., De Backer, E., Temmerman, M., and Vaneechoutte, M. (2005). Comparison between Gram Stain and Culture for the Characterization of Vaginal Microflora: Definition of a Distinct Grade That Resembles Grade I Microflora and Revised Categorization of Grade I Microflora. BMC Microbiol., 5.","DOI":"10.1186\/1471-2180-5-61"},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Shipitsyna, E., Roos, A., Datcu, R., Hall\u00e9n, A., Fredlund, H., Jensen, J.S., Engstrand, L., and Unemo, M. (2013). Composition of the Vaginal Microbiota in Women of Reproductive Age\u2014Sensitive and Specific Molecular Diagnosis of Bacterial Vaginosis Is Possible?. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0060670"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1016\/j.anaerobe.2017.05.012","article-title":"Quantification, Isolation and Characterization of Bifidobacterium from the Vaginal Microbiomes of Reproductive Aged Women","volume":"47","author":"Freitas","year":"2017","journal-title":"Anaerobe"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1186\/2049-2618-2-23","article-title":"Characterization of the Vaginal Microbiota of Healthy Canadian Women through the Menstrual Cycle","volume":"2","author":"Chaban","year":"2014","journal-title":"Microbiome"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1215","DOI":"10.1016\/0002-9378(94)90135-X","article-title":"Desquamative Inflammatory Vaginitis: A New Subgroup of Purulent Vaginitis Responsive to Topical 2% Clindamycin Therapy","volume":"171","author":"Sobel","year":"1994","journal-title":"Am. J. Obstet. Gynecol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1097\/QCO.0b013e3282f3d999","article-title":"Global Epidemiology and Control of Trichomonas Vaginalis","volume":"21","author":"Johnston","year":"2008","journal-title":"Curr. Opin. Infect. Dis."},{"key":"ref_11","first-page":"295","article-title":"Phenotypic and Genotypic Study of Streptococcus Agalactiae in Vagina of Pregnant Women in Argentina","volume":"61","author":"Toresani","year":"2001","journal-title":"Medicina"},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"El Aila, N.A., Tency, I., Claeys, G., Saerens, B., De Backer, E., Temmerman, M., Verhelst, R., and Vaneechoutte, M. (2009). Genotyping of Streptococcus Agalactiae(Group B Streptococci) Isolated from Vaginal and Rectal Swabs of Women at 35\u201337 Weeks of Pregnancy. BMC Infect. Dis., 9.","DOI":"10.1186\/1471-2334-9-153"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1155\/S1064744901000047","article-title":"Vaginal Microflora Associated with Bacterial Vaginosis in Nonpregnant Women: Reliability of Sialidase Detection","volume":"9","author":"Smayevsky","year":"2001","journal-title":"Infect. Dis. Obstet. Gynecol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1130","DOI":"10.1016\/j.ajog.2004.04.013","article-title":"Culture-Independent Analysis of Vaginal Microflora: The Unrecognized Association of Atopobium Vaginae with Bacterial Vaginosis","volume":"191","author":"Verstraelen","year":"2004","journal-title":"Am. J. Obstet. Gynecol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"629","DOI":"10.1111\/j.1542-2011.2012.00246.x","article-title":"Vaginal Infections Update","volume":"57","author":"Mashburn","year":"2012","journal-title":"J. Midwifery Women\u2019s Health"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"413","DOI":"10.1136\/sti.78.6.413","article-title":"Validation of a Simplified Grading of Gram Stained Vaginal Smears for Use in Genitourinary Medicine Clinics","volume":"78","author":"Ison","year":"2002","journal-title":"Sex. Transm. Infect."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"De Backer, E., Verhelst, R., Verstraelen, H., Alqumber, M.A., Burton, J.P., Tagg, J.R., Temmerman, M., and Vaneechoutte, M. (2007). Quantitative Determination by Real-Time PCR of Four Vaginal Lactobacillus Species, Gardnerella Vaginalis and Atopobium Vaginae Indicates an Inverse Relationship between L. Gasseri and L. Iners. BMC Microbiol., 7.","DOI":"10.1186\/1471-2180-7-115"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1745","DOI":"10.1128\/jcm.26.9.1745-1753.1988","article-title":"Optimal Data Processing Procedure for Automatic Bacterial Identification by Gas-Liquid Chromatography of Cellular Fatty Acids","volume":"26","author":"Eerola","year":"1988","journal-title":"J. Clin. Microbiol."},{"key":"ref_19","first-page":"79","article-title":"Nugent Score Related to Vaginal Culture in Pregnant Women","volume":"98","author":"Delaney","year":"2001","journal-title":"Obstet. Gynecol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1016\/j.ijms.2009.12.015","article-title":"Comparison of MALDI-TOF\/MS and LC-QTOF\/MS Methods for the Identification of Enteric Bacteria","volume":"291","author":"Mott","year":"2010","journal-title":"Int. J. Mass Spectrom."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"R49","DOI":"10.1111\/j.1365-2621.2005.tb07149.x","article-title":"Real-Time Polymerase Chain Reaction for the Food Microbiologist: Technologies, Applications, and Limitations","volume":"70","author":"Hanna","year":"2005","journal-title":"J. Food Sci."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1016\/S0167-7012(02)00035-0","article-title":"Identification of Lactobacillus Species Using TDNA-PCR","volume":"50","author":"Baele","year":"2002","journal-title":"J. Microbiol. Methods"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"729","DOI":"10.1002\/jrs.1250221203","article-title":"Application of Surface-Enhanced Raman Spectroscopy to Biological Systems","volume":"22","author":"Cotton","year":"1991","journal-title":"J. Raman Spectrosc."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Koglin, E., and S\u00e9quaris, J.-M. (1986). Surface Enhanced Raman Scattering of Biomolecules, Springer.","DOI":"10.1007\/3-540-16403-0_1"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"2290","DOI":"10.1021\/ac00020a018","article-title":"Borate Interference in Surface-Enhanced Raman Spectroscopy of Amines","volume":"63","author":"Herne","year":"1991","journal-title":"Anal. Chem."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1002\/jrs.1250190408","article-title":"Surface-Enhanced Raman Scattering of Nucleic Acid Components in Silver Sol: Uracil and Its Derivatives","volume":"19","author":"Oh","year":"1988","journal-title":"J. Raman Spectrosc."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"2911","DOI":"10.1021\/acssensors.1c00641","article-title":"Wide-Range, Rapid, and Specific Identification of Pathogenic Bacteria by Surface-Enhanced Raman Spectroscopy","volume":"6","author":"Liu","year":"2021","journal-title":"ACS Sens."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"119989","DOI":"10.1016\/j.saa.2021.119989","article-title":"Surface-Enhanced Raman Spectroscopy for Identification of Food Processing Bacteria","volume":"261","author":"Kashif","year":"2021","journal-title":"Spectrochim. Acta Part A Mol. Biomol. Spectrosc."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"5677","DOI":"10.1021\/acs.analchem.8b05533","article-title":"Identification of Newly Emerging Influenza Viruses by Detecting the Virally Infected Cells Based on Surface Enhanced Raman Spectroscopy and Principal Component Analysis","volume":"91","author":"Lim","year":"2019","journal-title":"Anal. Chem."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"11652","DOI":"10.1021\/acs.analchem.5b02661","article-title":"Identification of Newly Emerging Influenza Viruses by Surface-Enhanced Raman Spectroscopy","volume":"87","author":"Lim","year":"2015","journal-title":"Anal. Chem."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"2484","DOI":"10.1021\/acs.analchem.7b03124","article-title":"Characterization of Clinically Relevant Fungi via SERS Fingerprinting Assisted by Novel Chemometric Models","volume":"90","author":"Dina","year":"2018","journal-title":"Anal. Chem."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"108990","DOI":"10.1016\/j.ijfoodmicro.2020.108990","article-title":"Label-Free Surface Enhanced Raman Scattering Spectroscopy for Discrimination and Detection of Dominant Apple Spoilage Fungus","volume":"338","author":"Guo","year":"2021","journal-title":"Int. J. Food Microbiol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"594","DOI":"10.1128\/JCM.40.2.594-600.2002","article-title":"Rapid Identification of Candida Species by Confocal Raman Microspectroscopy","volume":"40","author":"Maquelin","year":"2002","journal-title":"J. Clin. Microbiol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1276","DOI":"10.1366\/000370209789806849","article-title":"Characterization of Yeast Species Using Surface-Enhanced Raman Scattering","volume":"63","author":"Sayin","year":"2009","journal-title":"Appl. Spectrosc."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"8427","DOI":"10.1039\/C6AY02957D","article-title":"Detection and Identification of Human Fungal Pathogens Using Surface-Enhanced Raman Spectroscopy and Principal Component Analysis","volume":"8","author":"Witkowska","year":"2016","journal-title":"Anal. Methods"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"117223","DOI":"10.1016\/j.saa.2019.117223","article-title":"Yeast Cell Wall\u2014Silver Nanoparticles Interaction: A Synergistic Approach between Surface-Enhanced Raman Scattering and Computational Spectroscopy Tools","volume":"222","author":"Gherman","year":"2019","journal-title":"Spectrochim. Acta Part A Mol. Biomol. Spectrosc."},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Pan, Y.L., Yang, T.S., Chang, T.C., and Chang, H.C. (2009, January 18\u201321). Rapid Identification of Candida Albicans Based on Raman Spectral Biosensing Technology. Proceedings of the 2009 IEEE 3rd International Conference Nano\/Molecular Medicine and Engineering, Tainan, Taiwan.","DOI":"10.1109\/NANOMED.2009.5559104"},{"key":"ref_38","first-page":"1180","article-title":"Understanding and Discrimination of Biofilms of Clinically Relevant Microorganisms Using Surface-Enhanced Raman Scattering","volume":"71","year":"2016","journal-title":"Appl. Spectrosc."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"110765","DOI":"10.1016\/j.colsurfb.2019.110765","article-title":"Biofilm Formation of Clinically Important Microorganisms on 2D and 3D Poly (Methyl Methacrylate) Substrates: A Surface-Enhanced Raman Scattering Study","volume":"188","year":"2020","journal-title":"Colloids Surf. B Biointerfaces"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1061","DOI":"10.1016\/j.saa.2014.08.155","article-title":"Effect of Toxicity of Ag Nanoparticles on SERS Spectral Variance of Bacteria","volume":"137","author":"Cui","year":"2015","journal-title":"Spectrochim. Acta Part A Mol. Biomol. Spectrosc."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"113358","DOI":"10.1016\/j.bios.2021.113358","article-title":"SERS-Based Sensor for the Detection of Sexually Transmitted Pathogens in the Male Swab Specimens: A New Approach for Clinical Diagnosis","volume":"189","author":"Berus","year":"2021","journal-title":"Biosens. Bioelectron."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1496","DOI":"10.1016\/j.jmrt.2021.03.083","article-title":"Ultrasensitive SERS Platform Made via Femtosecond Laser Micromachining for Biomedical Applications","volume":"12","author":"Szymborski","year":"2021","journal-title":"J. Mater. Res. Technol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1099\/00207713-38-1-12","article-title":"Lactobacillus Kefiranofaciens Sp. Nov. Isolated from Kefir Grains","volume":"38","author":"Fujisawa","year":"1988","journal-title":"Int. J. Syst. Bacteriol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1016\/S0924-2244(99)00033-3","article-title":"Bifidobacterium Spp. and Lactobacillus Acidophilus: Biological, Biochemical, Technological and Therapeutical Properties Relevant for Use as Probiotics","volume":"10","author":"Gomes","year":"1999","journal-title":"Trends Food Sci. Technol."},{"key":"ref_45","first-page":"426","article-title":"Septic Arthritis of the Knee Joint Secondary to Prevotella Bivia","volume":"30","author":"Salman","year":"2009","journal-title":"Saudi Med. J."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1016\/j.tim.2004.05.008","article-title":"The Distinct Morphogenic States of Candida Albicans","volume":"12","author":"Sudbery","year":"2004","journal-title":"Trends Microbiol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"2874","DOI":"10.1039\/C6AN00306K","article-title":"The Origin of the Band at around 730 Cm-1 in the SERS Spectra of Bacteria: A Stable Isotope Approach","volume":"141","author":"Kubryk","year":"2016","journal-title":"Analyst"},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Gieroba, B., Sroka-Bartnicka, A., Kazimierczak, P., Kalisz, G., Pieta, I.S., Nowakowski, R., Pisarek, M., and Przekora, A. (2020). Effect of Gelation Temperature on the Molecular Structure and Physicochemical Properties of the Curdlan Matrix: Spectroscopic and Microscopic Analyses. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21176154"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"27789","DOI":"10.1038\/srep27789","article-title":"Label-Free Chemical Imaging of Fungal Spore Walls by Raman Microscopy and Multivariate Curve Resolution Analysis","volume":"6","author":"Noothalapati","year":"2016","journal-title":"Sci. Rep."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"103901","DOI":"10.1063\/1.2891086","article-title":"Surface Enhanced Raman Scattering Analyses of Individual Silver Nanoaggregates on Living Single Yeast Cell Wall","volume":"92","author":"Sujith","year":"2008","journal-title":"Appl. Phys. Lett."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1007\/s10719-008-9157-1","article-title":"Structural Details and Composition of Trichomonas Vaginalis Lipophosphoglycan in Relevance to the Epithelial Immune Function","volume":"26","author":"Singh","year":"2009","journal-title":"Glycoconj. J."},{"key":"ref_52","unstructured":"Ghonaim, H.M., Periasamy, N., Noro, M.G., and Anwar, J. (2022, August 08). Towards a Simplified Model Membrane of Skin Lipids: Preparation and Characterisation of a Ternary Lipid Mixture. Available online: https:\/\/eprints.lancs.ac.uk\/id\/eprint\/73840\/."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1080\/05704928.2014.923902","article-title":"Raman Spectroscopy of Biological Tissues","volume":"50","author":"Talari","year":"2015","journal-title":"Appl. Spectrosc. Rev."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/23\/20\/12576\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T00:57:51Z","timestamp":1760144271000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/23\/20\/12576"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,10,20]]},"references-count":53,"journal-issue":{"issue":"20","published-online":{"date-parts":[[2022,10]]}},"alternative-id":["ijms232012576"],"URL":"https:\/\/doi.org\/10.3390\/ijms232012576","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,10,20]]}}}