{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T08:37:15Z","timestamp":1762072635504,"version":"build-2065373602"},"reference-count":39,"publisher":"MDPI AG","issue":"21","license":[{"start":{"date-parts":[[2022,10,24]],"date-time":"2022-10-24T00:00:00Z","timestamp":1666569600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32071982"],"award-info":[{"award-number":["32071982"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Hybrids between different subspecies of rice Oryza\u00a0sativa L. commonly show hybrid sterility. Here we show that a widely planted commercial japonica\/GJ variety, DHX2, exhibited hybrid sterility when crossing with other GJ varieties. Using the high-quality genome assembly, we identified three copies of the Sc gene in DHX2, whereas Nipponbare (Nip) had only one copy of Sc. Knocking out the extra copies of Sc in DHX2 significantly improved the pollen fertility of the F1 plant of DHX2\/Nip cross. The population structure analysis revealed that a slight introgression from Basmati1 might occur in the genome of DHX2. We demonstrated that both DHX2 and Basmati1 harbored three copies of Sc. Moreover, the introgression of GS3 and BADH2\/fgr from Basmati1 confers the slender and fragrance grain of DHX2. These results add to our understanding of the hybrid sterility of inter-subspecies and intra-subspecies and may provide a novel strategy for hybrid breeding.<\/jats:p>","DOI":"10.3390\/ijms232112804","type":"journal-article","created":{"date-parts":[[2022,10,24]],"date-time":"2022-10-24T10:09:23Z","timestamp":1666606163000},"page":"12804","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Introgression of a Complex Genomic Structural Variation Causes Hybrid Male Sterility in GJ Rice (Oryza sativa L.) Subspecies"],"prefix":"10.3390","volume":"23","author":[{"given":"Na","family":"Xu","sequence":"first","affiliation":[{"name":"Rice Research Institute, Shenyang Agricultural University, Shenyang 110866, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hai","family":"Xu","sequence":"additional","affiliation":[{"name":"Rice Research Institute, Shenyang Agricultural University, Shenyang 110866, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhengjin","family":"Xu","sequence":"additional","affiliation":[{"name":"Rice Research Institute, Shenyang Agricultural University, Shenyang 110866, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fengcheng","family":"Li","sequence":"additional","affiliation":[{"name":"Rice Research Institute, Shenyang Agricultural University, Shenyang 110866, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2698-5314","authenticated-orcid":false,"given":"Quan","family":"Xu","sequence":"additional","affiliation":[{"name":"Rice Research Institute, Shenyang Agricultural University, Shenyang 110866, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,10,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1038\/s41586-018-0063-9","article-title":"Genomic variation in 3010 diverse accessions of Asian cultivated rice","volume":"557","author":"Wang","year":"2018","journal-title":"Nature"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"629","DOI":"10.1038\/nature19760","article-title":"Genomic architecture of heterosis for yield traits in rice","volume":"537","author":"Huang","year":"2016","journal-title":"Nature"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"186","DOI":"10.1016\/j.pbi.2010.01.002","article-title":"Hybrid sterility in plant: Stories from rice","volume":"13","author":"Ouyang","year":"2010","journal-title":"Curr. 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