{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,30]],"date-time":"2026-08-30T12:45:56Z","timestamp":1788093956024,"version":"build-2803163510"},"reference-count":48,"publisher":"MDPI AG","issue":"21","license":[{"start":{"date-parts":[[2022,10,26]],"date-time":"2022-10-26T00:00:00Z","timestamp":1666742400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["SCHW 703\/3-1"],"award-info":[{"award-number":["SCHW 703\/3-1"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["R124\/16-1"],"award-info":[{"award-number":["R124\/16-1"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["SFB-TR 84"],"award-info":[{"award-number":["SFB-TR 84"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"name":"HMWK LOEWE Research Cluster Diffusible Signals","award":["SCHW 703\/3-1"],"award-info":[{"award-number":["SCHW 703\/3-1"]}]},{"name":"HMWK LOEWE Research Cluster Diffusible Signals","award":["R124\/16-1"],"award-info":[{"award-number":["R124\/16-1"]}]},{"name":"HMWK LOEWE Research Cluster Diffusible Signals","award":["SFB-TR 84"],"award-info":[{"award-number":["SFB-TR 84"]}]},{"name":"Open Access Publikationskosten","award":["SCHW 703\/3-1"],"award-info":[{"award-number":["SCHW 703\/3-1"]}]},{"name":"Open Access Publikationskosten","award":["R124\/16-1"],"award-info":[{"award-number":["R124\/16-1"]}]},{"name":"Open Access Publikationskosten","award":["SFB-TR 84"],"award-info":[{"award-number":["SFB-TR 84"]}]},{"name":"Heidelberg University","award":["SCHW 703\/3-1"],"award-info":[{"award-number":["SCHW 703\/3-1"]}]},{"name":"Heidelberg University","award":["R124\/16-1"],"award-info":[{"award-number":["R124\/16-1"]}]},{"name":"Heidelberg University","award":["SFB-TR 84"],"award-info":[{"award-number":["SFB-TR 84"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>The human central nervous system (CNS) is separated from the blood by distinct cellular barriers, including the blood\u2013brain barrier (BBB) and the blood\u2013cerebrospinal fluid (CFS) barrier (BCSFB). Whereas at the center of the BBB are the endothelial cells of the brain capillaries, the BCSFB is formed by the epithelium of the choroid plexus. Invasion of cells of either the BBB or the BCSFB is a potential first step during CNS entry by the Gram-positive bacterium Listeria monocytogenes (Lm). Lm possesses several virulence factors mediating host cell entry, such as the internalin protein family\u2014including internalin (InlA), which binds E-cadherin (Ecad) on the surface of target cells, and internalin B (InlB)\u2014interacting with the host cell receptor tyrosine kinase Met. A further family member is internalin (InlF), which targets the intermediate filament protein vimentin. Whereas InlF has been shown to play a role during brain invasion at the BBB, its function during infection at the BCSFB is not known. We use human brain microvascular endothelial cells (HBMEC) and human choroid plexus epithelial papilloma (HIBCPP) cells to investigate the roles of InlF and vimentin during CNS invasion by Lm. Whereas HBMEC present intracellular and surface vimentin (besides Met), HIBCPP cells do not express vimentin (except Met and Ecad). Treatment with the surface vimentin modulator withaferin A (WitA) inhibited invasion of Lm into HBMEC, but not HIBCPP cells. Invasion of Lm into HBMEC and HIBCPP cells is, however, independent of InlF, since a deletion mutant of Lm lacking InlF did not display reduced invasion rates.<\/jats:p>","DOI":"10.3390\/ijms232112908","type":"journal-article","created":{"date-parts":[[2022,10,26]],"date-time":"2022-10-26T05:11:33Z","timestamp":1666761093000},"page":"12908","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Different Involvement of Vimentin during Invasion by Listeria monocytogenes at the Blood\u2013Brain and the Blood\u2013Cerebrospinal Fluid Barriers In Vitro"],"prefix":"10.3390","volume":"23","author":[{"given":"Franjo","family":"Banovic","sequence":"first","affiliation":[{"name":"Pediatric Infectious Diseases, Department of Pediatrics, Medical Faculty Mannheim, Heidelberg University, 68167 Mannheim, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8191-1890","authenticated-orcid":false,"given":"Sandrin","family":"Schulze","sequence":"additional","affiliation":[{"name":"Pediatric Infectious Diseases, Department of Pediatrics, Medical Faculty Mannheim, Heidelberg University, 68167 Mannheim, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mobarak","family":"Abu Mraheil","sequence":"additional","affiliation":[{"name":"Institute of Medical Microbiology, German Center for Infection Research (DZIF), Partner Site Giessen-Marburg-Langen, Justus-Liebig University Giessen, 35392 Giessen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Torsten","family":"Hain","sequence":"additional","affiliation":[{"name":"Institute of Medical Microbiology, German Center for Infection Research (DZIF), Partner Site Giessen-Marburg-Langen, Justus-Liebig University Giessen, 35392 Giessen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1452-4759","authenticated-orcid":false,"given":"Trinad","family":"Chakraborty","sequence":"additional","affiliation":[{"name":"Institute of Medical Microbiology, German Center for Infection Research (DZIF), Partner Site Giessen-Marburg-Langen, Justus-Liebig University Giessen, 35392 Giessen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V\u00e9ronique","family":"Orian-Rousseau","sequence":"additional","affiliation":[{"name":"Institute of Biological and Chemical Systems-Functional Molecular Systems (IBCS-FMS), Karlsruhe Institute of Technology (KIT), 76344 Eggenstein-Leopoldshafen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Selina","family":"Moroniak","sequence":"additional","affiliation":[{"name":"Pediatric Infectious Diseases, Department of Pediatrics, Medical Faculty Mannheim, Heidelberg University, 68167 Mannheim, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christel","family":"Weiss","sequence":"additional","affiliation":[{"name":"Department of Medical Statistics and Biomathematics, Medical Faculty Mannheim, Heidelberg University, 68167 Mannheim, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroshi","family":"Ishikawa","sequence":"additional","affiliation":[{"name":"Laboratory of Clinical Regenerative Medicine, Department of Neurosurgery, Faculty of Medicine, University of Tsukuba, Tsukuba 305-8575, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Horst","family":"Schroten","sequence":"additional","affiliation":[{"name":"Pediatric Infectious Diseases, Department of Pediatrics, Medical Faculty Mannheim, Heidelberg University, 68167 Mannheim, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2150-8199","authenticated-orcid":false,"given":"R\u00fcdiger","family":"Adam","sequence":"additional","affiliation":[{"name":"Pediatric Infectious Diseases, Department of Pediatrics, Medical Faculty Mannheim, Heidelberg University, 68167 Mannheim, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1706-0259","authenticated-orcid":false,"given":"Christian","family":"Schwerk","sequence":"additional","affiliation":[{"name":"Pediatric Infectious Diseases, Department of Pediatrics, Medical Faculty Mannheim, Heidelberg University, 68167 Mannheim, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,10,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1038\/nrmicro.2017.126","article-title":"Listeria monocytogenes: Towards a complete picture of its physiology and pathogenesis","volume":"16","author":"Radoshevich","year":"2018","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Banovic, F., Schroten, H., and Schwerk, C. (2020). Potential Roles and Functions of Listerial Virulence Factors during Brain Entry. Toxins, 12.","DOI":"10.3390\/toxins12050297"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1056\/NEJM198301273080407","article-title":"Epidemic listeriosis--evidence for transmission by food","volume":"308","author":"Schlech","year":"1983","journal-title":"N. Engl. J. Med."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1111\/j.1574-695X.2008.00404.x","article-title":"Listeria monocytogenes: Epidemiology, human disease, and mechanisms of brain invasion","volume":"53","author":"Drevets","year":"2008","journal-title":"FEMS Immunol. Med. Microbiol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"136","DOI":"10.3389\/fcimb.2018.00136","article-title":"To Be Cytosolic or Vacuolar: The Double Life of Listeria monocytogenes","volume":"8","author":"Bierne","year":"2018","journal-title":"Front. Cell. Infect. Microbiol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"232","DOI":"10.1128\/IAI.66.1.232-238.1998","article-title":"Listeria monocytogenes virulence factors that stimulate endothelial cells","volume":"66","author":"Drevets","year":"1998","journal-title":"Infect. Immun."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1006\/mpat.1995.0029","article-title":"Bacteremia is required for invasion of the murine central nervous system by Listeria monocytogenes","volume":"18","author":"Berche","year":"1995","journal-title":"Microb. Pathog."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"213","DOI":"10.4161\/viru.19586","article-title":"Targeting of the central nervous system by Listeria monocytogenes","volume":"3","author":"Disson","year":"2012","journal-title":"Virulence"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"691","DOI":"10.1128\/CMR.00118-13","article-title":"Pathogens Penetrating the Central Nervous System: Infection Pathways and the Cellular and Molecular Mechanisms of Invasion","volume":"27","author":"Dando","year":"2014","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1016\/j.micinf.2012.12.005","article-title":"The surface proteins InlA and InlB are interdependently required for polar basolateral invasion by Listeria monocytogenes in a human model of the blood-cerebrospinal fluid barrier","volume":"15","author":"Grundler","year":"2013","journal-title":"Microbes Infect. Inst. Pasteur"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1007\/s00401-018-1807-1","article-title":"Molecular anatomy and functions of the choroidal blood-cerebrospinal fluid barrier in health and disease","volume":"135","author":"Strazielle","year":"2018","journal-title":"Acta Neuropathol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"5723","DOI":"10.1113\/JP275376","article-title":"Physiology and molecular biology of barrier mechanisms in the fetal and neonatal brain","volume":"596","author":"Saunders","year":"2018","journal-title":"J. Physiol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"5260","DOI":"10.1128\/IAI.66.11.5260-5267.1998","article-title":"Interaction of Listeria monocytogenes with human brain microvascular endothelial cells: InlB-dependent invasion, long-term intracellular growth, and spread from macrophages to endothelial cells","volume":"66","author":"Greiffenberg","year":"1998","journal-title":"Infect. Immun."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"557","DOI":"10.1046\/j.1365-2958.2002.02767.x","article-title":"InlA- but not InlB-mediated internalization of Listeria monocytogenes by non-phagocytic mammalian cells needs the support of other internalins","volume":"43","author":"Bergmann","year":"2002","journal-title":"Mol. Microbiol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1016\/j.micinf.2016.09.003","article-title":"Mitogen-activated protein kinases are required for effective infection of human choroid plexus epithelial cells by Listeria monocytogenes","volume":"19","author":"Dinner","year":"2017","journal-title":"Microbes Infect. Inst. Pasteur"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1156","DOI":"10.1016\/j.micinf.2007.05.003","article-title":"Internalins: A complex family of leucine-rich repeat-containing proteins in Listeria monocytogenes","volume":"9","author":"Bierne","year":"2007","journal-title":"Microbes Infect. Inst. Pasteur"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"379","DOI":"10.4161\/viru.2.5.17703","article-title":"The arsenal of virulence factors deployed by Listeria monocytogenes to promote its cell infection cycle","volume":"2","author":"Camejo","year":"2011","journal-title":"Virulence"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"923","DOI":"10.1016\/S0092-8674(00)81070-3","article-title":"E-cadherin is the receptor for internalin, a surface protein required for entry of L. monocytogenes into epithelial cells","volume":"84","author":"Mengaud","year":"1996","journal-title":"Cell"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"501","DOI":"10.1016\/S0092-8674(00)00141-0","article-title":"InIB-dependent internalization of Listeria is mediated by the Met receptor tyrosine kinase","volume":"103","author":"Shen","year":"2000","journal-title":"Cell"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"3275","DOI":"10.1128\/IAI.68.6.3275-3279.2000","article-title":"Interaction of Listeria monocytogenes with human brain microvascular endothelial cells: An electron microscopic study","volume":"68","author":"Greiffenberg","year":"2000","journal-title":"Infect. Immun."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1128\/IAI.71.1.95-100.2003","article-title":"Antibodies present in normal human serum inhibit invasion of human brain microvascular endothelial cells by Listeria monocytogenes","volume":"71","author":"Hertzig","year":"2003","journal-title":"Infect. Immun."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1615","DOI":"10.1128\/iai.65.5.1615-1625.1997","article-title":"Identification of four new members of the internalin multigene family of Listeria monocytogenes EGD","volume":"65","author":"Dramsi","year":"1997","journal-title":"Infect. Immun."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"e00160-18","DOI":"10.1128\/mBio.00160-18","article-title":"Invasion of the Brain by Listeria monocytogenes Is Mediated by InlF and Host Cell Vimentin","volume":"9","author":"Ghosh","year":"2018","journal-title":"mBio"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1571","DOI":"10.1091\/mbc.E18-04-0228","article-title":"Matrix stiffness modulates infection of endothelial cells by Listeria monocytogenes via expression of cell surface vimentin","volume":"29","author":"Bastounis","year":"2018","journal-title":"Mol. Biol. Cell"},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Huang, S.H., Chi, F., Peng, L., Bo, T., Zhang, B., Liu, L.Q., Wu, X., Mor-Vaknin, N., Markovitz, D.M., and Cao, H. (2016). Vimentin, a Novel NF-kappaB Regulator, Is Required for Meningitic Escherichia coli K1-Induced Pathogen Invasion and PMN Transmigration across the Blood-Brain Barrier. PLoS ONE, 11.","DOI":"10.1371\/journal.pone.0162641"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"278","DOI":"10.2174\/1568026616666160829162313","article-title":"C-Met Inhibitors are Potential Novel Therapeutic Agents Against Listeria monocytogenes Infection Through Blocking the Bacteria Entry into Nonphagocytic Cells","volume":"17","author":"Puthiyakunnon","year":"2017","journal-title":"Curr. Top. Med. Chem."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Mak, T.N., and Bruggemann, H. (2016). Vimentin in Bacterial Infections. Cells, 5.","DOI":"10.3390\/cells5020018"},{"key":"ref_28","first-page":"e54061","article-title":"A Choroid Plexus Epithelial Cell-based Model of the Human Blood-Cerebrospinal Fluid Barrier to Study Bacterial Infection from the Basolateral Side","volume":"111","author":"Dinner","year":"2016","journal-title":"J. Vis. Exp. JoVE"},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Schwerk, C., Papandreou, T., Schuhmann, D., Nickol, L., Borkowski, J., Steinmann, U., Quednau, N., Stump, C., Weiss, C., and Berger, J. (2012). Polar Invasion and Translocation of Neisseria meningitidis and Streptococcus suis in a Novel Human Model of the Blood-Cerebrospinal Fluid Barrier. PLoS ONE, 7.","DOI":"10.1371\/journal.pone.0030069"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1006\/mpat.2000.0406","article-title":"Bacterial invasion and transcytosis in transfected human brain microvascular endothelial cells","volume":"30","author":"Stins","year":"2001","journal-title":"Microb. Pathog."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1111\/j.1749-0774.2005.tb00059.x","article-title":"Establishment and characterization of a human malignant choroids plexus papilloma cell line (HIBCPP)","volume":"18","author":"Ishiwata","year":"2005","journal-title":"Hum. Cell"},{"key":"ref_32","first-page":"231","article-title":"Intermediate filament proteins in choroid plexus and ependyma and their tumors","volume":"123","author":"Miettinen","year":"1986","journal-title":"Am. J. Pathol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1007\/BF00710822","article-title":"Coexistence of cytokeratin, vimentin and neurofilament protein in human choroid plexus. An immunohistochemical study of intermediate filaments in neuroepithelial tissues","volume":"410","author":"Kasper","year":"1986","journal-title":"Virchows. Arch. A Pathol. Anat. Histopathol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"264","DOI":"10.1080\/01926230490274326","article-title":"A case report of a choroid plexus carcinoma spontaneously occurring in the right lateral ventricle of a 14-week-old, female Donryu rat","volume":"32","author":"Shimomoto","year":"2004","journal-title":"Toxicol. Pathol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1186\/s12987-016-0027-0","article-title":"Modeling immune functions of the mouse blood-cerebrospinal fluid barrier in vitro: Primary rather than immortalized mouse choroid plexus epithelial cells are suited to study immune cell migration across this brain barrier","volume":"13","author":"Lazarevic","year":"2016","journal-title":"Fluids Barriers CNS"},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Danielsson, F., Peterson, M.K., Caldeira Araujo, H., Lautenschlager, F., and Gad, A.K.B. (2018). Vimentin Diversity in Health and Disease. Cells, 7.","DOI":"10.3390\/cells7100147"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"e2000078","DOI":"10.1002\/bies.202000078","article-title":"Mechanical and Non-Mechanical Functions of Filamentous and Non-Filamentous Vimentin","volume":"42","author":"Patteson","year":"2020","journal-title":"BioEssays News Rev. Mol. Cell. Dev. Biol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1042\/BJ20091097","article-title":"Vimentin-mediated signalling is required for IbeA+ E. coli K1 invasion of human brain microvascular endothelial cells","volume":"427","author":"Chi","year":"2010","journal-title":"Biochem. J."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1897","DOI":"10.1128\/JVI.01249-13","article-title":"Cellular vimentin regulates construction of dengue virus replication complexes through interaction with NS4A protein","volume":"88","author":"Teo","year":"2014","journal-title":"J. Virol."},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Deng, L., Spencer, B.L., Holmes, J.A., Mu, R., Rego, S., Weston, T.A., Hu, Y., Sanches, G.F., Yoon, S., and Park, N. (2019). The Group B Streptococcal surface antigen I\/II protein, BspC, interacts with host vimentin to promote adherence to brain endothelium and inflammation during the pathogenesis of meningitis. PLoS Pathog., 15.","DOI":"10.1101\/544395"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1046\/j.1365-2958.1998.00776.x","article-title":"Internalin B is essential for adhesion and mediates the invasion of Listeria monocytogenes into human endothelial cells","volume":"28","author":"Parida","year":"1998","journal-title":"Mol. Microbiol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"446","DOI":"10.1038\/nrm1128","article-title":"Rocks: Multifunctional kinases in cell behaviour","volume":"4","author":"Riento","year":"2003","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"749","DOI":"10.1111\/j.1365-2958.2008.06188.x","article-title":"Inhibition of ROCK activity allows InlF-mediated invasion and increased virulence of Listeria monocytogenes","volume":"68","author":"Kirchner","year":"2008","journal-title":"Mol. Microbiol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"748461","DOI":"10.3389\/fcimb.2021.748461","article-title":"inlF Enhances Listeria monocytogenes Early-Stage Infection by Inhibiting the Inflammatory Response","volume":"11","author":"Ling","year":"2021","journal-title":"Front. Cell. Infect. Microbiol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"234","DOI":"10.1128\/iai.45.1.234-241.1984","article-title":"Acquired resistance to facultative intracellular bacteria: Relationship between persistence, cross-reactivity at the T-cell level, and capacity to stimulate cellular immunity of different Listeria strains","volume":"45","author":"Kaufmann","year":"1984","journal-title":"Infect. Immun."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"3896","DOI":"10.1128\/iai.63.10.3896-3903.1995","article-title":"Expression of the Listeria monocytogenes EGD inlA and inlB genes, whose products mediate bacterial entry into tissue culture cell lines, by PrfA-dependent and -independent mechanisms","volume":"63","author":"Lingnau","year":"1995","journal-title":"Infect. Immun."},{"key":"ref_47","first-page":"720","article-title":"Vector plasmid for insertional mutagenesis and directional cloning in Listeria spp.","volume":"19","author":"Schaferkordt","year":"1995","journal-title":"Biotechniques"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"671","DOI":"10.1038\/nmeth.2089","article-title":"NIH Image to ImageJ: 25 years of image analysis","volume":"9","author":"Schneider","year":"2012","journal-title":"Nat. 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