{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,9]],"date-time":"2026-05-09T07:01:06Z","timestamp":1778310066015,"version":"3.51.4"},"reference-count":56,"publisher":"MDPI AG","issue":"21","license":[{"start":{"date-parts":[[2022,10,26]],"date-time":"2022-10-26T00:00:00Z","timestamp":1666742400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Natural Science Foundation","award":["31860686"],"award-info":[{"award-number":["31860686"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>The hypopharyngeal gland is an important organ for honey bees to secrete royal jelly, and its secretory activity varies with the age of workers. However, by now, the regulation mechanism of hypopharyngeal gland development is still unclear. Here, the expression profiles of miRNAs in the hypopharyngeal gland of newly emerged workers, nurses, and foragers were investigated via small RNA sequencing. From these three stages, 81 known miRNAs and 135 novel miRNAs have been identified. A total of 85 miRNAs showed expression differences between different development stages, and their target genes were predicted to range from 1 to more than 10. Many of the differentially expressed miRNAs and target genes are related to growth and development or apoptosis. Moreover, dual-luciferase-reporter assays verified that novel-miR-11 directly targets the 3\u2032-untranslated regions of LOC410685 (inactive tyrosine-protein kinase 7) and LOC725318 (uncharacterized protein). These results suggested that miRNAs were widely involved in the developmental regulation of the hypopharyngeal gland in honey bees.<\/jats:p>","DOI":"10.3390\/ijms232112970","type":"journal-article","created":{"date-parts":[[2022,10,26]],"date-time":"2022-10-26T09:59:37Z","timestamp":1666778377000},"page":"12970","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Expression Profile of microRNAs during Development of the Hypopharyngeal Gland in Honey Bee, Apis mellifera"],"prefix":"10.3390","volume":"23","author":[{"given":"Kaixin","family":"Qin","sequence":"first","affiliation":[{"name":"Honeybee Research Institute, Jiangxi Agricultural University, Nanchang 330045, China"},{"name":"Jiangxi Province Key Laboratory of Honeybee Biology and Beekeeping, Nanchang 330045, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fuping","family":"Cheng","sequence":"additional","affiliation":[{"name":"Honeybee Research Institute, Jiangxi Agricultural University, Nanchang 330045, China"},{"name":"Jiangxi Province Key Laboratory of Honeybee Biology and Beekeeping, Nanchang 330045, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luxia","family":"Pan","sequence":"additional","affiliation":[{"name":"Honeybee Research Institute, Jiangxi Agricultural University, Nanchang 330045, China"},{"name":"Jiangxi Province Key Laboratory of Honeybee Biology and Beekeeping, Nanchang 330045, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zilong","family":"Wang","sequence":"additional","affiliation":[{"name":"Honeybee Research Institute, Jiangxi Agricultural University, Nanchang 330045, China"},{"name":"Jiangxi Province Key Laboratory of Honeybee Biology and Beekeeping, Nanchang 330045, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,10,26]]},"reference":[{"key":"ref_1","unstructured":"Wu, J., Yan, J.C., and Xiao, B. 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