{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T02:44:00Z","timestamp":1792809840300,"version":"4.5.0"},"reference-count":65,"publisher":"MDPI AG","issue":"21","license":[{"start":{"date-parts":[[2022,10,30]],"date-time":"2022-10-30T00:00:00Z","timestamp":1667088000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32070316"],"award-info":[{"award-number":["32070316"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31950410557"],"award-info":[{"award-number":["31950410557"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Secondary cell wall thickening plays a crucial role in plant growth and development. Diploid woodland strawberry (Fragaria vesca) is an excellent model for studying fruit development, but its molecular control of secondary wall thickening is largely unknown. Previous studies have shown that Arabidopsis NAC secondary wall thickening promoting factor1 (AtNST1) and related proteins are master regulators of xylem fiber cell differentiation in multiple plant species. In this study, a NST1-like gene, FvNST1b, was isolated and characterized from strawberry. Sequence alignment and phylogenetic analysis showed that the FvNST1b protein contains a highly conserved NAC domain, and it belongs to the same family as AtNST1. Overexpression of FvNST1b in wild-type Arabidopsis caused extreme dwarfism, induced ectopic thickening of secondary walls in various tissues, and upregulated the expression of genes related to secondary cell wall synthesis. In addition, transient overexpression of FvNST1b in wild-type Fragaria vesca fruit produced cells resembling tracheary elements. These results suggest that FvNST1b positively regulates secondary cell wall formation as orthologous genes from other species.<\/jats:p>","DOI":"10.3390\/ijms232113212","type":"journal-article","created":{"date-parts":[[2022,10,30]],"date-time":"2022-10-30T07:26:50Z","timestamp":1667114810000},"page":"13212","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["FvNST1b NAC Protein Induces Secondary Cell Wall Formation in Strawberry"],"prefix":"10.3390","volume":"23","author":[{"given":"Xiaofei","family":"Dang","sequence":"first","affiliation":[{"name":"College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bei","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chen","family":"Li","sequence":"additional","affiliation":[{"name":"College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shingo","family":"Nagawa","sequence":"additional","affiliation":[{"name":"Fujian Agriculture and Forestry University\u2013University of California, Riverside, Joint Center for Horticultural Biology and Metabolomics, Haixia Institute of Science and Technology, Fujian Agriculture and Forestry University, Fuzhou 350002, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,10,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1046\/j.1365-313X.2003.01729.x","article-title":"Reduced cellulose synthesis invokes lignification and defense responses in Arabidopsis thaliana","volume":"34","author":"Penfield","year":"2003","journal-title":"Plant J."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"2993","DOI":"10.1105\/tpc.105.036004","article-title":"The NAC Transcription Factors NST1 and NST2 of Arabidopsis Regulate Secondary Wall Thickenings and Are Required for Anther Dehiscence","volume":"17","author":"Mitsuda","year":"2005","journal-title":"Plant Cell"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1039\/b716663j","article-title":"Cellulose fibrils direct plant organ movements","volume":"139","author":"Fratzl","year":"2008","journal-title":"Faraday Discuss."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"768","DOI":"10.1111\/j.1365-313X.2008.03633.x","article-title":"NAC transcription factors NST1 and NST3 regulate pod shattering in a partially redundant manner by promoting secondary wall formation after the establishment of tissue identity","volume":"56","author":"Mitsuda","year":"2008","journal-title":"Plant J."},{"key":"ref_5","unstructured":"Mauseth, J. 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