{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,3]],"date-time":"2026-04-03T17:31:50Z","timestamp":1775237510954,"version":"3.50.1"},"reference-count":49,"publisher":"MDPI AG","issue":"21","license":[{"start":{"date-parts":[[2022,10,30]],"date-time":"2022-10-30T00:00:00Z","timestamp":1667088000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006769","name":"Russian Science Foundation","doi-asserted-by":"publisher","award":["22-25-00420"],"award-info":[{"award-number":["22-25-00420"]}],"id":[{"id":"10.13039\/501100006769","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Mutations in surface proteins enable emerging variants of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) to escape a substantial fraction of neutralizing antibodies and may thus weaken vaccine-driven immunity. To compare available vaccines and justify revaccination, rapid evaluation of antibody (Ab) responses to currently circulating SARS-CoV-2 variants of interest (VOI) and concern (VOC) is needed. Here, we developed a multiplex protein microarray-based system for rapid profiling of anti-SARS-CoV-2 Ab levels in human sera. The microarray system was validated using sera samples from SARS-CoV-2-free donors and those diagnosed with COVID-19 based on PCR and enzyme immunoassays. Microarray-based profiling of vaccinated donors revealed a substantial difference in anti-VOC Ab levels elicited by the replication-deficient adenovirus vector-base (Sputnik V) and whole-virion (CoviVac Russia COVID-19) vaccines. Whole-virion vaccine-induced Abs showed minor but statistically significant cross-reactivity with the human blood coagulation factor 1 (fibrinogen) and thrombin. However, their effects on blood clotting were negligible, according to thrombin time tests, providing evidence against the concept of pronounced cross-reactivity-related side effects of the vaccine. Importantly, all samples were collected in the pre-Omicron period but showed noticeable responses to the receptor-binding domain (RBD) of the Omicron spike protein. Thus, using the new express Ab-profiling system, we confirmed the inter-variant cross-reactivity of the anti-SARS-CoV-2 Abs and demonstrated the relative potency of the vaccines against new VOCs.<\/jats:p>","DOI":"10.3390\/ijms232113220","type":"journal-article","created":{"date-parts":[[2022,10,30]],"date-time":"2022-10-30T07:26:50Z","timestamp":1667114810000},"page":"13220","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Microarray Profiling of Vaccination-Induced Antibody Responses to SARS-CoV-2 Variants of Interest and Concern"],"prefix":"10.3390","volume":"23","author":[{"given":"Julia","family":"Svetlova","sequence":"first","affiliation":[{"name":"Federal Research and Clinical Center of Physical-Chemical Medicine, Malaya Pirogovskaya, 1a, 119435 Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dmitry","family":"Gustin","sequence":"additional","affiliation":[{"name":"Lomonosov Institute of Fine Chemical Technologies, MIREA\u2014Russian Technological University, Vernadsky Avenue, 86, 119454 Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2471-0563","authenticated-orcid":false,"given":"Valentin","family":"Manuvera","sequence":"additional","affiliation":[{"name":"Federal Research and Clinical Center of Physical-Chemical Medicine, Malaya Pirogovskaya, 1a, 119435 Moscow, Russia"},{"name":"Moscow Institute of Physics and Technology (State University), 9, Institutskiy per., Moscow Region, 141701 Dolgoprudny, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dmitriy","family":"Shirokov","sequence":"additional","affiliation":[{"name":"Federal Research and Clinical Center of Physical-Chemical Medicine, Malaya Pirogovskaya, 1a, 119435 Moscow, Russia"},{"name":"K. I. Skryabin Moscow State Academy of Veterinary Medicine and Biotechnology, Akademika Skryabina Street, 23, 109472 Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Varvara","family":"Shokina","sequence":"additional","affiliation":[{"name":"Scientific Research Institute for Systems Biology and Medicine, Scientific Driveway, 18, 117246 Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kirill","family":"Prusakov","sequence":"additional","affiliation":[{"name":"Federal Research and Clinical Center of Physical-Chemical Medicine, Malaya Pirogovskaya, 1a, 119435 Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Konstantin","family":"Aldarov","sequence":"additional","affiliation":[{"name":"Federal Research and Clinical Center of Physical-Chemical Medicine, Malaya Pirogovskaya, 1a, 119435 Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daria","family":"Kharlampieva","sequence":"additional","affiliation":[{"name":"Federal Research and Clinical Center of Physical-Chemical Medicine, Malaya Pirogovskaya, 1a, 119435 Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9535-5792","authenticated-orcid":false,"given":"Daria","family":"Matyushkina","sequence":"additional","affiliation":[{"name":"Scientific Research Institute for Systems Biology and Medicine, Scientific Driveway, 18, 117246 Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4408-503X","authenticated-orcid":false,"given":"Julia","family":"Bespyatykh","sequence":"additional","affiliation":[{"name":"Federal Research and Clinical Center of Physical-Chemical Medicine, Malaya Pirogovskaya, 1a, 119435 Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9359-8651","authenticated-orcid":false,"given":"Anna","family":"Varizhuk","sequence":"additional","affiliation":[{"name":"Federal Research and Clinical Center of Physical-Chemical Medicine, Malaya Pirogovskaya, 1a, 119435 Moscow, Russia"},{"name":"Moscow Institute of Physics and Technology (State University), 9, Institutskiy per., Moscow Region, 141701 Dolgoprudny, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vassili","family":"Lazarev","sequence":"additional","affiliation":[{"name":"Federal Research and Clinical Center of Physical-Chemical Medicine, Malaya Pirogovskaya, 1a, 119435 Moscow, Russia"},{"name":"Moscow Institute of Physics and Technology (State University), 9, Institutskiy per., Moscow Region, 141701 Dolgoprudny, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7356-397X","authenticated-orcid":false,"given":"Tatiana","family":"Vedekhina","sequence":"additional","affiliation":[{"name":"Federal Research and Clinical Center of Physical-Chemical Medicine, Malaya Pirogovskaya, 1a, 119435 Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,10,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"461","DOI":"10.1016\/j.molonc.2010.09.003","article-title":"Reverse Phase Protein Microarrays Advance to Use in Clinical Trials","volume":"4","author":"Mueller","year":"2010","journal-title":"Mol. 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