{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T06:20:29Z","timestamp":1790662829811,"version":"4.1.0"},"reference-count":56,"publisher":"MDPI AG","issue":"21","license":[{"start":{"date-parts":[[2022,11,2]],"date-time":"2022-11-02T00:00:00Z","timestamp":1667347200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Fundaci\u00f3n S\u00e9neca\u2013Murcia Regional Agency for Science and Technology","award":["20855\/PI\/18"],"award-info":[{"award-number":["20855\/PI\/18"]}]},{"name":"Fundaci\u00f3n S\u00e9neca\u2013Murcia Regional Agency for Science and Technology","award":["FI21\/00003"],"award-info":[{"award-number":["FI21\/00003"]}]},{"name":"Fundaci\u00f3n S\u00e9neca\u2013Murcia Regional Agency for Science and Technology","award":["PI20\/00505"],"award-info":[{"award-number":["PI20\/00505"]}]},{"name":"Fundaci\u00f3n S\u00e9neca\u2013Murcia Regional Agency for Science and Technology","award":["CP19\/00098"],"award-info":[{"award-number":["CP19\/00098"]}]},{"name":"Institute of Health \u201cCarlos III\u201d (ISCIII)","award":["20855\/PI\/18"],"award-info":[{"award-number":["20855\/PI\/18"]}]},{"name":"Institute of Health \u201cCarlos III\u201d (ISCIII)","award":["FI21\/00003"],"award-info":[{"award-number":["FI21\/00003"]}]},{"name":"Institute of Health \u201cCarlos III\u201d (ISCIII)","award":["PI20\/00505"],"award-info":[{"award-number":["PI20\/00505"]}]},{"name":"Institute of Health \u201cCarlos III\u201d (ISCIII)","award":["CP19\/00098"],"award-info":[{"award-number":["CP19\/00098"]}]},{"name":"Miguel Servet Type I","award":["20855\/PI\/18"],"award-info":[{"award-number":["20855\/PI\/18"]}]},{"name":"Miguel Servet Type I","award":["FI21\/00003"],"award-info":[{"award-number":["FI21\/00003"]}]},{"name":"Miguel Servet Type I","award":["PI20\/00505"],"award-info":[{"award-number":["PI20\/00505"]}]},{"name":"Miguel Servet Type I","award":["CP19\/00098"],"award-info":[{"award-number":["CP19\/00098"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Acute myeloid leukemia (AML) is a cancer of the myeloid blood cells mainly treated with chemotherapy for cancer remission, but this non-selective treatment also induces numerous side effects. Investigations with bioactive compounds from plant-derived foods against cancer have increased in the last years because there is an urgent need to search for new anti-leukemic agents possessing higher efficacy and selectivity for AML cells and fewer negative side effects. In this study, we analyzed the anti-leukemic activity of several phytochemicals that are representative of the major classes of compounds present in cruciferous foods (glucosinolates, isothiocyanates, hydroxycinnamic acids, flavonols, and anthocyanins) in the human acute myeloid leukemia cell line HL-60. Our results revealed that among the different Brassica-derived compounds assayed, sulforaphane (SFN) (an aliphatic isothiocyanate) showed the most potent anti-leukemic activity with an IC50 value of 6 \u00b5M in dose-response MTT assays after 48 h of treatment. On the other hand, chlorogenic acid (a hydroxycinnamic acid) and cyanidin-3-glucoside (an anthocyanin) also displayed anti-leukemic potential, with IC50 values of 7 \u00b5M and 17 \u00b5M after 48 h of incubation, respectively. Importantly, these compounds did not show significant cell toxicity in macrophages-like differentiated cells at 10 and 25 \u00b5M, indicating that their cytotoxic effects were specific to AML cancer cells. Finally, we found that these three compounds were able to induce the NRF2\/KEAP1 signaling pathway in a dose-dependent manner, highlighting SFN as the most potent NRF2 activator. Overall, the present evidence shed light on the potential for using foods and ingredients rich in anticancer bioactive phytochemicals from Brassica spp.<\/jats:p>","DOI":"10.3390\/ijms232113400","type":"journal-article","created":{"date-parts":[[2022,11,3]],"date-time":"2022-11-03T03:11:21Z","timestamp":1667445081000},"page":"13400","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Anti-Leukemic Activity of Brassica-Derived Bioactive Compounds in HL-60 Myeloid Leukemia Cells"],"prefix":"10.3390","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8938-2767","authenticated-orcid":false,"given":"Mar\u00eda \u00c1ngeles","family":"N\u00fa\u00f1ez-S\u00e1nchez","sequence":"first","affiliation":[{"name":"Obesity and Metabolism Laboratory, Biomedical Research Institute of Murcia (IMIB), 30120 Murcia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda Antonia","family":"Mart\u00ednez-S\u00e1nchez","sequence":"additional","affiliation":[{"name":"Obesity and Metabolism Laboratory, Biomedical Research Institute of Murcia (IMIB), 30120 Murcia, Spain"},{"name":"Department of Biochemistry, Molecular Biology B and Immunology, School of Medicine, University of Murcia, Regional Campus of International Excellence \u201cCampus Mare Nostrum\u201d, 30100 Murcia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marina","family":"Verdejo-S\u00e1nchez","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Molecular Biology B and Immunology, School of Medicine, University of Murcia, Regional Campus of International Excellence \u201cCampus Mare Nostrum\u201d, 30100 Murcia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2117-3130","authenticated-orcid":false,"given":"Paula","family":"Garc\u00eda-Ib\u00e1\u00f1ez","sequence":"additional","affiliation":[{"name":"Aquaporins Research Group, Department of Plant Nutrition, Centro de Edafolog\u00eda y Biolog\u00eda Aplicada del Segura (CEBAS), CSIC, Campus de Espinardo-25, 30100 Murcia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alba","family":"Oliva Bolar\u00edn","sequence":"additional","affiliation":[{"name":"Obesity and Metabolism Laboratory, Biomedical Research Institute of Murcia (IMIB), 30120 Murcia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6804-5449","authenticated-orcid":false,"given":"Bruno","family":"Ramos-Molina","sequence":"additional","affiliation":[{"name":"Obesity and Metabolism Laboratory, Biomedical Research Institute of Murcia (IMIB), 30120 Murcia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6547-8764","authenticated-orcid":false,"given":"Diego A.","family":"Moreno","sequence":"additional","affiliation":[{"name":"Phytochemistry and Healthy Food Lab (LabFAS), Department of Food Science Technology, Centro de Edafolog\u00eda y Biolog\u00eda Aplicada del Segura (CEBAS), CSIC, Campus de Espinardo-25, 30100 Murcia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6913-0610","authenticated-orcid":false,"given":"Antonio J.","family":"Ruiz-Alcaraz","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Molecular Biology B and Immunology, School of Medicine, University of Murcia, Regional Campus of International Excellence \u201cCampus Mare Nostrum\u201d, 30100 Murcia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,11,2]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1002\/ajh.24246","article-title":"Acute myeloid leukemia-Major progress over four decades and glimpses into the future","volume":"91","author":"Kantarjian","year":"2016","journal-title":"Am. 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