{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T04:41:21Z","timestamp":1760244081624,"version":"build-2065373602"},"reference-count":52,"publisher":"MDPI AG","issue":"21","license":[{"start":{"date-parts":[[2022,11,7]],"date-time":"2022-11-07T00:00:00Z","timestamp":1667779200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Consejo Nacional de Ciencia y Tecnolog\u00eda (CONACyT FOSSIS)","award":["261670","47301018","320221"],"award-info":[{"award-number":["261670","47301018","320221"]}]},{"name":"Universidad Aut\u00f3noma Metropolitana (UAM)","award":["261670","47301018","320221"],"award-info":[{"award-number":["261670","47301018","320221"]}]},{"name":"F003 PCC","award":["261670","47301018","320221"],"award-info":[{"award-number":["261670","47301018","320221"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>The clinical phenotype of LMNA-associated dilated cardiomyopathy (DCM) varies even among individuals who share the same mutation. LMNA encodes lamin AC, which interacts with the lamin-associated protein 2 alpha (LAP2\u03b1) encoded by the TMPO gene. The LAP2\u03b1\/Arg690Cys polymorphism is frequent in Latin America and was previously found to disrupt LAP2\u03b1-Lamin AC interactions in vitro. We identified a DCM patient heterozygous for both a lamin AC truncating mutation (Ser431*) and the LAP2\u03b1\/Arg690Cys polymorphism. We performed protein modeling and docking experiments, and used confocal microscopy to compare leukocyte nuclear morphology among family members with different genotype combinations (wild type, LAP2\u03b1 Arg690Cys heterozygous, lamin AC\/Ser431* heterozygous, and LAP2\u03b1 Arg690Cys\/lamin AC Ser431* double heterozygous). Protein modeling predicted that 690Cys destabilizes the LAP2\u03b1 homodimer and impairs lamin AC-LAP2\u03b1 docking. Lamin AC-deficient nuclei (Ser431* heterozygous) showed characteristic blebs and invaginations, significantly decreased nuclear area, and increased elongation, while LAP2\u03b1\/Arg690Cys heterozygous nuclei showed a lower perimeter and higher circularity than wild-type nuclei. LAP2\u03b1 Arg690Cys apparently attenuated the effect of LMNA Ser431* on the nuclear area and fully compensated for its effect on nuclear circularity. Altogether, the data suggest that LAP2\u03b1\/Arg690Cys may be one of the many factors contributing to phenotype variation of LMNA-associated DCM.<\/jats:p>","DOI":"10.3390\/ijms232113626","type":"journal-article","created":{"date-parts":[[2022,11,7]],"date-time":"2022-11-07T03:10:46Z","timestamp":1667790646000},"page":"13626","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Leukocyte Nuclear Morphology Alterations in Dilated Cardiomyopathy Caused by a Lamin AC Truncating Mutation (LMNA\/Ser431*) Are Modified by the Presence of a LAP2 Missense Polymorphism (TMPO\/Arg690Cys)"],"prefix":"10.3390","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2578-3155","authenticated-orcid":false,"given":"Antonia","family":"Gonz\u00e1lez-Garrido","sequence":"first","affiliation":[{"name":"Laboratorio de Enfermedades Mendelianas, Instituto Nacional de Medicina Gen\u00f3mica, Mexico City 14610, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sandra","family":"Rosas-Madrigal","sequence":"additional","affiliation":[{"name":"Laboratorio de Enfermedades Mendelianas, Instituto Nacional de Medicina Gen\u00f3mica, Mexico City 14610, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arturo","family":"Rojo-Dom\u00ednguez","sequence":"additional","affiliation":[{"name":"Departamento de Ciencias Naturales, Universidad Aut\u00f3noma Metropolitana, Unidad Cuajimalpa, Mexico City 05348, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7363-4870","authenticated-orcid":false,"given":"Jaime","family":"Arellanes-Robledo","sequence":"additional","affiliation":[{"name":"Laboratorio de Enfermedades Hep\u00e1ticas, Instituto Nacional de Medicina Gen\u00f3mica, Mexico City 14610, Mexico"},{"name":"Direcci\u00f3n de C\u00e1tedras, Consejo Nacional de Ciencia y Tecnolog\u00eda (CONACyT), Mexico City 03940, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Enrique","family":"L\u00f3pez-Mora","sequence":"additional","affiliation":[{"name":"Cl\u00ednica de Insuficiencia Cardiaca, Instituto Nacional de Cardiolog\u00eda \u201cIgnacio Ch\u00e1vez\u201d, Mexico City 14080, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alessandra","family":"Carnevale","sequence":"additional","affiliation":[{"name":"Laboratorio de Enfermedades Mendelianas, Instituto Nacional de Medicina Gen\u00f3mica, Mexico City 14610, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leticia","family":"Arregui","sequence":"additional","affiliation":[{"name":"Departamento de Ciencias Naturales, Universidad Aut\u00f3noma Metropolitana, Unidad Cuajimalpa, Mexico City 05348, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rigoberto","family":"Rosendo-Guti\u00e9rrez","sequence":"additional","affiliation":[{"name":"Laboratorio de Enfermedades Mendelianas, Instituto Nacional de Medicina Gen\u00f3mica, Mexico City 14610, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sandra","family":"Romero-Hidalgo","sequence":"additional","affiliation":[{"name":"Departmento de Gen\u00f3mica Computacional, Instituto Nacional de Medicina Gen\u00f3mica, Mexico City 14610, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4450-7690","authenticated-orcid":false,"given":"Mar\u00eda Teresa","family":"Villarreal-Molina","sequence":"additional","affiliation":[{"name":"Laboratorio de Gen\u00f3mica Cardiovascular, Instituto Nacional de Medicina Gen\u00f3mica, Mexico City 14610, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,11,7]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1186\/s13073-017-0410-8","article-title":"Genetics and genomics of dilated cardiomyopathy and systolic heart failure","volume":"9","author":"Tayal","year":"2017","journal-title":"Genome Med."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"362","DOI":"10.1111\/joim.12944","article-title":"Dilated cardiomyopathy: From epidemiologic to genetic phenotypes: A translational review of current literature","volume":"286","author":"Reichart","year":"2019","journal-title":"J. Intern. Med."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"731","DOI":"10.1161\/CIRCRESAHA.116.309396","article-title":"Dilated Cardiomyopathy: Genetic Determinants and Mechanisms","volume":"121","author":"McNally","year":"2017","journal-title":"Circ. Res."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Barp, A., Bello, L., Politano, L., Melacini, P., Calore, C., Polo, A., Vianello, S., Sorar\u00f9, G., Semplicini, C., and Pantic, B. (2015). Genetic Modifiers of Duchenne Muscular Dystrophy and Dilated Cardiomyopathy. PLoS ONE, 10.","DOI":"10.1371\/journal.pone.0141240"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"e1049","DOI":"10.1002\/mgg3.1049","article-title":"Mutations in PDLIM5 are rare in dilated cardiomyopathy but are emerging as potential disease modifiers","volume":"8","author":"Verdonschot","year":"2020","journal-title":"Mol. Genet. Genom. Med."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1302","DOI":"10.1161\/CIRCULATIONAHA.120.050432","article-title":"Genetic Variation in Enhancers Modifies Cardiomyopathy Gene Expression and Progression","volume":"143","author":"Gacita","year":"2021","journal-title":"Circulation"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1641","DOI":"10.1016\/j.jacc.2011.01.015","article-title":"Update 2011: Clinical and Genetic Issues in Familial Dilated Cardiomyopathy","volume":"57","author":"Hershberger","year":"2011","journal-title":"J. Am. Coll. Cardiol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"981","DOI":"10.1016\/S0735-1097(02)01724-2","article-title":"Autosomal dominant dilated cardiomyopathy with atrioventricular block: A lamin A\/C defect-related disease","volume":"39","author":"Arbustini","year":"2002","journal-title":"J. Am. Coll. Cardiol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"389","DOI":"10.1038\/s41431-018-0294-0","article-title":"Assessment of fibroblast nuclear morphology aids interpretation of LMNA variants","volume":"27","author":"Lindsey","year":"2019","journal-title":"Eur. J. Hum. Genet."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"e021101","DOI":"10.1161\/JAHA.121.021101","article-title":"Blood-Based Epigenetic Markers of FKBP5 Gene Methylation in Patients with Dilated Cardiomyopathy","volume":"10","author":"Wada","year":"2021","journal-title":"J. Am. Heart Assoc."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Ferradini, V., Cosma, J., Romeo, F., De Masi, C., Murdocca, M., Spitalieri, P., Mannucci, S., Parlapiano, G., Di Lorenzo, F., and Martino, A. (2021). Clinical Features of LMNA-Related Cardiomyopathy in 18 Patients and Characterization of Two Novel Variants. J. Clin. Med., 10.","DOI":"10.3390\/jcm10215075"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"826","DOI":"10.1002\/mus.1076","article-title":"Nuclear alterations in autosomal-dominant Emery-Dreifuss muscular dystrophy","volume":"24","author":"Sabatelli","year":"2001","journal-title":"Muscle Nerve"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1059","DOI":"10.1126\/science.1127168","article-title":"Lamin A-Dependent Nuclear Defects in Human Aging","volume":"312","author":"Scaffidi","year":"2006","journal-title":"Science"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"8703","DOI":"10.1073\/pnas.0602569103","article-title":"Mutant nuclear lamin A leads to progressive alterations of epigenetic control in premature aging","volume":"103","author":"Shumaker","year":"2006","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"2479","DOI":"10.1093\/hmg\/ddl170","article-title":"Pathology and nuclear abnormalities in hearts of transgenic mice expressing M371K lamin A encoded by an LMNA mutation causing Emery-Dreifuss muscular dystrophy","volume":"15","author":"Wang","year":"2006","journal-title":"Hum. Mol. Genet."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1869","DOI":"10.1016\/j.yexcr.2008.02.018","article-title":"Expression of the myodystrophic R453W mutation of lamin A in C2C12 myoblasts causes promoter-specific and global epigenetic defects","volume":"314","author":"Delbarre","year":"2008","journal-title":"Exp. Cell Res."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1016\/j.nmd.2008.09.018","article-title":"Nuclear changes in skeletal muscle extend to satellite cells in autosomal dominant Emery-Dreifuss muscular dystrophy\/limb-girdle muscular dystrophy 1B","volume":"19","author":"Park","year":"2009","journal-title":"Neuromuscul. Disord."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"365","DOI":"10.1007\/s00395-010-0085-4","article-title":"Genetic and ultrastructural studies in dilated cardiomyopathy patients: A large deletion in the lamin A\/C gene is associated with cardiomyocyte nuclear envelope disruption","volume":"105","author":"Gupta","year":"2010","journal-title":"Basic Res. Cardiol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"011503","DOI":"10.1063\/5.0082656","article-title":"Nuclear lamins: Structure and function in mechanobiology","volume":"6","author":"Vahabikashi","year":"2022","journal-title":"APL Bioeng."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"694","DOI":"10.1152\/physiolgenomics.00128.2017","article-title":"Mechanisms of allelic and clinical heterogeneity of lamin A\/C phenotypes","volume":"50","author":"Hoffman","year":"2018","journal-title":"Physiol. Genom."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1038\/nrm1550","article-title":"The nuclear lamina comes of age","volume":"6","author":"Gruenbaum","year":"2005","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"832","DOI":"10.1101\/gad.1652708","article-title":"Nuclear lamins: Major factors in the structural organization and function of the nucleus and chromatin","volume":"22","author":"Dechat","year":"2008","journal-title":"Genes Dev."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"335ra58","DOI":"10.1126\/scitranslmed.aad4991","article-title":"Laminopathies disrupt epigenomic developmental programs and cell fate","volume":"8","author":"Perovanovic","year":"2016","journal-title":"Sci. Transl. Med."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1356","DOI":"10.3389\/fphys.2018.01356","article-title":"Role of Lamin A\/C Gene Mutations in the Signaling Defects Leading to Cardiomyopathies","volume":"9","author":"Gerbino","year":"2018","journal-title":"Front. Physiol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"626","DOI":"10.1016\/j.devcel.2009.10.016","article-title":"The Nuclear Envelope as a Signaling Node in Development and Disease","volume":"17","author":"Dauer","year":"2009","journal-title":"Dev. Cell"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"517","DOI":"10.1101\/sqb.2010.75.014","article-title":"Role of the Nuclear Lamina in Genome Organization and Gene Expression","volume":"75","year":"2010","journal-title":"Cold Spring Harb. Symp. Quant. Biol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"3473","DOI":"10.1242\/jcs.113.19.3473","article-title":"Lamina-associated polypeptide 2alpha binds intranuclear A-type lamins","volume":"113","author":"Dechat","year":"2000","journal-title":"J. Cell Sci."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"761","DOI":"10.3389\/fphys.2020.00761","article-title":"The Broad Spectrum of LMNA Cardiac Diseases: From Molecular Mechanisms to Clinical Phenotype","volume":"11","author":"Crasto","year":"2020","journal-title":"Front. Physiol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"a000554","DOI":"10.1101\/cshperspect.a000554","article-title":"Lamin-binding Proteins","volume":"2","author":"Wilson","year":"2010","journal-title":"Cold Spring Harb. Perspect. Biol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"2087","DOI":"10.1242\/jcs.203430","article-title":"Lamins in the nuclear interior\u2014Life outside the lamina","volume":"130","author":"Naetar","year":"2017","journal-title":"J. Cell Sci."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"566","DOI":"10.1002\/humu.20250","article-title":"Thymopoietin (lamina-associated polypeptide 2) gene mutation associated with dilated cardiomyopathy","volume":"26","author":"Taylor","year":"2005","journal-title":"Hum. Mutat."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"647343","DOI":"10.3389\/fgene.2021.647343","article-title":"Interaction of HLA Class II rs9272219 and TMPO rs17028450 (Arg690Cys) Variants Affects Neuromyelitis Optica Spectrum Disorder Susceptibility in an Admixed Mexican Population","volume":"12","author":"Barquera","year":"2021","journal-title":"Front. Genet."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Saj, M., Bilinska, Z.T., Tarnowska, A., Sioma, A., Bolongo, P., Sobieszczanska-Malek, M., Michalak, E., Golen, D., Mazurkiewicz, L., and Malek, L. (2013). LMNA mutations in Polish patients with dilated cardiomyopathy: Prevalence, clinical characteristics, and in vitro studies. BMC Med. Genet., 14.","DOI":"10.1186\/1471-2350-14-55"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"346","DOI":"10.1111\/boc.201400033","article-title":"Role of nuclear Lamin A\/C in cardiomyocyte functions","volume":"106","author":"Carmosino","year":"2014","journal-title":"Biol. Cell"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1016\/j.yjmcc.2007.11.008","article-title":"Lamin A\/C haploinsufficiency causes dilated cardiomyopathy and apoptosis-triggered cardiac conduction system disease","volume":"44","author":"Wolf","year":"2008","journal-title":"J. Mol. Cell. Cardiol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1007\/s00109-007-0275-1","article-title":"Incomplete nonsense-mediated decay of mutant lamin A\/C mRNA provokes dilated cardiomyopathy and ventricular tachycardia","volume":"86","author":"Geiger","year":"2007","journal-title":"J. Mol. Med."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"3010","DOI":"10.1016\/j.yexcr.2013.08.024","article-title":"The LMNA mutation p.Arg321Ter associated with dilated cardiomyopathy leads to reduced expression and a skewed ratio of lamin A and lamin C proteins","volume":"319","author":"Rasmussen","year":"2013","journal-title":"Exp. Cell Res."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1016\/j.ijcard.2019.09.058","article-title":"Expression of LMNA-R225X nonsense mutation results in dilated cardiomyopathy and conduction disorders (DCM-CD) in mice: Impact of exercise training","volume":"298","author":"Cai","year":"2020","journal-title":"Int. J. Cardiol."},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"1000 Genomes Project Consortium (2015). A global reference for human genetic variation. Nature, 526, 68\u201374.","DOI":"10.1038\/nature15393"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"462","DOI":"10.1101\/gr.196220.115","article-title":"A-type lamins bind both hetero- and euchromatin, the latter being regulated by lamina-associated poly-peptide 2 alpha","volume":"26","author":"Gesson","year":"2016","journal-title":"Genome Res."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1093\/ehjci\/jev014","article-title":"Recommendations for Cardiac Chamber Quantification by Echocardiography in Adults: An Update from the American Society of Echocardiography and the European Association of Cardiovascular Imaging","volume":"16","author":"Lang","year":"2015","journal-title":"Eur. Heart J. Cardiovasc. Imaging"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1556","DOI":"10.1038\/nprot.2015.105","article-title":"Genomic variant annotation and prioritization with ANNOVAR and wANNOVAR","volume":"10","author":"Yang","year":"2015","journal-title":"Nat. Protoc."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"e002480","DOI":"10.1161\/CIRCGEN.119.002480","article-title":"Variant Interpretation for Dilated Cardiomyopathy: Refinement of the American College of Medical Genetics and Genomics\/ClinGen Guidelines for the DCM Precision Medicine Study","volume":"13","author":"Morales","year":"2020","journal-title":"Circ. Genom. Precis. Med."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"1400","DOI":"10.1002\/jcc.20065","article-title":"Extending the treatment of backbone energetics in protein force fields: Limitations of gas-phase quantum mechanics in reproducing protein conformational distributions in molecular dynamics simulations","volume":"25","author":"Mackerell","year":"2004","journal-title":"J. Comput. Chem."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"811","DOI":"10.1016\/S0969-2126(02)00777-3","article-title":"The Ig-like Structure of the C-Terminal Domain of Lamin A\/C, Mutated in Muscular Dystrophies, Cardiomyopathy, and Partial Lipodystrophy","volume":"10","author":"Krimm","year":"2002","journal-title":"Structure"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"W445","DOI":"10.1093\/nar\/gkq311","article-title":"HexServer: An FFT-based protein docking server powered by graphics processors","volume":"38","author":"MacIndoe","year":"2010","journal-title":"Nucleic Acids Res."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"1771","DOI":"10.1093\/bioinformatics\/btu097","article-title":"ZDOCK server: Interactive docking prediction of protein-protein complexes and symmetric multimers","volume":"30","author":"Pierce","year":"2014","journal-title":"Bioinformatics"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"720","DOI":"10.1016\/j.jmb.2015.09.014","article-title":"The HADDOCK2.2 Web Server: User-Friendly Integrative Modeling of Biomolecular Complexes","volume":"428","author":"Rodrigues","year":"2016","journal-title":"J. Mol. Biol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"415","DOI":"10.1016\/0022-1759(84)90416-2","article-title":"Centrifugation of normal and rheumatoid arthritis blood on Ficoll-Hypaque and Ficoll-Nycodenz solutions","volume":"73","author":"McCarthy","year":"1984","journal-title":"J. Immunol. Methods"},{"key":"ref_50","first-page":"131","article-title":"Micromethod for rapid separation of lymphocytes from peripheral blood","volume":"1","author":"Fotino","year":"1971","journal-title":"Ann. Clin. Lab. Sci."},{"key":"ref_51","doi-asserted-by":"crossref","unstructured":"Ruitenberg, J.J., Mulder, C.B., Maino, V.C., Landay, A.L., and Ghanekar, S.A. (2006). VACUTAINER\u00ae CPT\u2122 and Ficoll density gradient separation perform equivalently in maintaining the quality and function of PBMC from HIV seropositive blood samples. BMC Immunol., 7.","DOI":"10.1186\/1471-2172-7-11"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1016\/j.bcp.2018.08.021","article-title":"Ethanol targets nucleoredoxin\/dishevelled interactions and stimulates phosphatidylinositol 4-phosphate production in vivo and in vitro","volume":"156","author":"Ibrahim","year":"2018","journal-title":"Biochem. Pharmacol."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/23\/21\/13626\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T01:11:42Z","timestamp":1760145102000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/23\/21\/13626"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,11,7]]},"references-count":52,"journal-issue":{"issue":"21","published-online":{"date-parts":[[2022,11]]}},"alternative-id":["ijms232113626"],"URL":"https:\/\/doi.org\/10.3390\/ijms232113626","relation":{},"ISSN":["1422-0067"],"issn-type":[{"type":"electronic","value":"1422-0067"}],"subject":[],"published":{"date-parts":[[2022,11,7]]}}}