{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T17:55:55Z","timestamp":1791914155985,"version":"4.3.4"},"reference-count":61,"publisher":"MDPI AG","issue":"22","license":[{"start":{"date-parts":[[2022,11,8]],"date-time":"2022-11-08T00:00:00Z","timestamp":1667865600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>The amphibious teleost Giant mudskipper (Periophthalmodon schlosseri, Pallas 1770) inhabit muddy plains and Asian mangrove forests. It spends more than 90% of its life outside of the water, using its skin, gills, and buccal-pharyngeal cavity mucosa to breathe in oxygen from the surrounding air. All vertebrates have been found to have mast cells (MCs), which are part of the innate immune system. These cells are mostly found in the mucous membranes of the organs that come in contact with the outside environment. According to their morphology, MCs have distinctive cytoplasmic granules that are released during the degranulation process. Additionally, these cells have antimicrobial peptides (AMPs) that fight a variety of infections. Piscidins, hepcidins, defensins, cathelicidins, and histonic peptides are examples of fish AMPs. Confocal microscopy was used in this study to assess Piscidin1 expression in Giant Mudskipper branchial MCs. Our results demonstrated the presence of MCs in the gills is highly positive for Piscidin1. Additionally, colocalized MCs labeled with TLR2\/5-HT and Piscidin1\/5-HT supported our data. The expression of Piscidin1 in giant mudskipper MCs highlights the involvement of this peptide in the orchestration of teleost immunity, advancing the knowledge of the defense system of this fish.<\/jats:p>","DOI":"10.3390\/ijms232213707","type":"journal-article","created":{"date-parts":[[2022,11,8]],"date-time":"2022-11-08T08:17:12Z","timestamp":1667895432000},"page":"13707","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Expression of Antimicrobic Peptide Piscidin1 in Gills Mast Cells of Giant Mudskipper Periophthalmodon schlosseri (Pallas, 1770)"],"prefix":"10.3390","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7580-8114","authenticated-orcid":false,"given":"Alessio","family":"Alesci","sequence":"first","affiliation":[{"name":"Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, 98166 Messina, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2002-1138","authenticated-orcid":false,"given":"Gioele","family":"Capillo","sequence":"additional","affiliation":[{"name":"Department of Biomedical, Dental and Morphological and Functional Imaging, University of Messina, 98125 Messina, Italy"},{"name":"Institute of Marine Biological Resources and Biotechnology, National Research Council (IRBIM, CNR), 98164 Messina, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7975-3926","authenticated-orcid":false,"given":"Doaa M.","family":"Mokhtar","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Histology, Faculty of Veterinary Medicine, Assiut University, Assiut 71526, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5815-5302","authenticated-orcid":false,"given":"Angelo","family":"Fumia","sequence":"additional","affiliation":[{"name":"Department of Clinical and Experimental Medicine, University of Messina, Padiglione C, AOU Policlinico \u2018G. Martino\u2019, 98124 Messina, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roberta","family":"D\u2019Angelo","sequence":"additional","affiliation":[{"name":"Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, 98166 Messina, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrizia","family":"Lo Cascio","sequence":"additional","affiliation":[{"name":"Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, 98166 Messina, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0635-2755","authenticated-orcid":false,"given":"Marco","family":"Albano","sequence":"additional","affiliation":[{"name":"Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, 98166 Messina, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Cristina","family":"Guerrera","sequence":"additional","affiliation":[{"name":"Department of Veterinary Sciences, University of Messina, 98168 Messina, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4467-6742","authenticated-orcid":false,"given":"Ramy K. A.","family":"Sayed","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Embryology, Faculty of Veterinary Medicine, Sohag University, Sohag 82524, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8360-4795","authenticated-orcid":false,"given":"Nunziacarla","family":"Span\u00f2","sequence":"additional","affiliation":[{"name":"Department of Biomedical, Dental and Morphological and Functional Imaging, University of Messina, 98125 Messina, Italy"},{"name":"Institute of Marine Biological Resources and Biotechnology, National Research Council (IRBIM, CNR), 98164 Messina, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6884-634X","authenticated-orcid":false,"given":"Simona","family":"Pergolizzi","sequence":"additional","affiliation":[{"name":"Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, 98166 Messina, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6132-6691","authenticated-orcid":false,"given":"Eugenia Rita","family":"Lauriano","sequence":"additional","affiliation":[{"name":"Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, 98166 Messina, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,11,8]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"380","DOI":"10.1016\/j.fsi.2018.01.014","article-title":"Immunohistochemical Characterization of Epidermal Dendritic-like Cells in Giant Mudskipper, Periophthalmodon Schlosseri","volume":"74","author":"Lauriano","year":"2018","journal-title":"Fish Shellfish Immunol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1016\/j.acthis.2013.05.005","article-title":"The Structural Organization and Immunohistochemistry of G-Protein Alpha Subunits in the Olfactory System of the Air-Breathing Mudskipper, Periophthalmus Barbarus (Linnaeus, 1766) (Gobiidae, Oxudercinae)","volume":"116","author":"Kuciel","year":"2014","journal-title":"Acta Histochem."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1016\/j.zool.2017.08.002","article-title":"Identification and Distribution of Neuronal Nitric Oxide Synthase and Neurochemical Markers in the Neuroepithelial Cells of the Gill and the Skin in the Giant Mudskipper, Periophthalmodon Schlosseri","volume":"125","author":"Zaccone","year":"2017","journal-title":"Zoology"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"383","DOI":"10.1111\/j.1095-8649.1990.tb05869.x","article-title":"Gross Structure of the Respiratory Organs and Dimensions of the Gill in the Mud\u2013Skipper, Periophthalmodon Schlosseri (Bleeker)","volume":"37","author":"Yadav","year":"1990","journal-title":"J. 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