{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T06:01:48Z","timestamp":1790402508514,"version":"4.1.0"},"reference-count":49,"publisher":"MDPI AG","issue":"22","license":[{"start":{"date-parts":[[2022,11,9]],"date-time":"2022-11-09T00:00:00Z","timestamp":1667952000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003852","name":"Regione Campania","doi-asserted-by":"publisher","award":["POR  2014-2020 ASSE III-Ob.Sp.14Az.10.5.2-\"Dotttorati di Ricerca con Caratterizzazione Industriale\""],"award-info":[{"award-number":["POR  2014-2020 ASSE III-Ob.Sp.14Az.10.5.2-\"Dotttorati di Ricerca con Caratterizzazione Industriale\""]}],"id":[{"id":"10.13039\/501100003852","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Glaucoma is a group of eye diseases consisting of optic nerve damage with corresponding loss of field vision and blindness. Hydrogen sulfide (H2S) is a gaseous neurotransmitter implicated in various pathophysiological processes. It is involved in the pathological mechanism of glaucomatous neuropathy and exerts promising effects in the treatment of this disease. In this work, we designed and synthetized new molecular hybrids between antiglaucoma drugs and H2S donors to combine the pharmacological effect of both moieties, providing a heightened therapy. Brinzolamide, betaxolol and brimonidine were linked to different H2S donors. The H2S-releasing properties of the new compounds were evaluated in a phosphate buffer solution by the amperometric approach, and evaluated in human primary corneal epithelial cells (HCEs) by spectrofluorometric measurements. Experimental data showed that compounds 1c, 1d and 3d were the hybrids with the best properties, characterized by a significant and long-lasting production of the gasotransmitter both in the aqueous solution (in the presence of L-cysteine) and in the intracellular environment. Because, to date, the donation of H2S by antiglaucoma H2S donor hybrids using non-immortalized corneal cells has never been reported, these results pave the way to further investigation of the potential efficacy of the newly synthesized compounds.<\/jats:p>","DOI":"10.3390\/ijms232213804","type":"journal-article","created":{"date-parts":[[2022,11,10]],"date-time":"2022-11-10T01:48:08Z","timestamp":1668044888000},"page":"13804","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Design, Synthesis and Evaluation of Novel Molecular Hybrids between Antiglaucoma Drugs and H2S Donors"],"prefix":"10.3390","volume":"23","author":[{"given":"Rosa","family":"Sparaco","sequence":"first","affiliation":[{"name":"Dipartimento di Farmacia, Universit\u00e0 degli Studi di Napoli \u201cFederico II\u201d, Via D. Montesano 49, 80131 Napoli, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valentina","family":"Citi","sequence":"additional","affiliation":[{"name":"Dipartimento di Farmacia, Universit\u00e0 di Pisa, Via Bonanno 6, 56126 Pisa, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elisa","family":"Magli","sequence":"additional","affiliation":[{"name":"Dipartimento di Sanit\u00e0 Pubblica, Universit\u00e0 Degli Studi di Napoli Federico II, Via Pansini 5, 80131 Napoli, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2090-7107","authenticated-orcid":false,"given":"Alma","family":"Martelli","sequence":"additional","affiliation":[{"name":"Dipartimento di Farmacia, Universit\u00e0 di Pisa, Via Bonanno 6, 56126 Pisa, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6558-3065","authenticated-orcid":false,"given":"Eugenia","family":"Piragine","sequence":"additional","affiliation":[{"name":"Dipartimento di Farmacia, Universit\u00e0 di Pisa, Via Bonanno 6, 56126 Pisa, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vincenzo","family":"Calderone","sequence":"additional","affiliation":[{"name":"Dipartimento di Farmacia, Universit\u00e0 di Pisa, Via Bonanno 6, 56126 Pisa, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giorgia","family":"Andreozzi","sequence":"additional","affiliation":[{"name":"Dipartimento di Farmacia, Universit\u00e0 degli Studi di Napoli \u201cFederico II\u201d, Via D. Montesano 49, 80131 Napoli, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1754-1191","authenticated-orcid":false,"given":"Elisa","family":"Perissutti","sequence":"additional","affiliation":[{"name":"Dipartimento di Farmacia, Universit\u00e0 degli Studi di Napoli \u201cFederico II\u201d, Via D. Montesano 49, 80131 Napoli, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8821-2937","authenticated-orcid":false,"given":"Francesco","family":"Frecentese","sequence":"additional","affiliation":[{"name":"Dipartimento di Farmacia, Universit\u00e0 degli Studi di Napoli \u201cFederico II\u201d, Via D. Montesano 49, 80131 Napoli, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vincenzo","family":"Santagada","sequence":"additional","affiliation":[{"name":"Dipartimento di Farmacia, Universit\u00e0 degli Studi di Napoli \u201cFederico II\u201d, Via D. Montesano 49, 80131 Napoli, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giuseppe","family":"Caliendo","sequence":"additional","affiliation":[{"name":"Dipartimento di Farmacia, Universit\u00e0 degli Studi di Napoli \u201cFederico II\u201d, Via D. Montesano 49, 80131 Napoli, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3887-8869","authenticated-orcid":false,"given":"Beatrice","family":"Severino","sequence":"additional","affiliation":[{"name":"Dipartimento di Farmacia, Universit\u00e0 degli Studi di Napoli \u201cFederico II\u201d, Via D. Montesano 49, 80131 Napoli, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0617-156X","authenticated-orcid":false,"given":"Angela","family":"Corvino","sequence":"additional","affiliation":[{"name":"Dipartimento di Farmacia, Universit\u00e0 degli Studi di Napoli \u201cFederico II\u201d, Via D. 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