{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T04:31:01Z","timestamp":1790742661794,"version":"4.1.0"},"reference-count":34,"publisher":"MDPI AG","issue":"22","license":[{"start":{"date-parts":[[2022,11,10]],"date-time":"2022-11-10T00:00:00Z","timestamp":1668038400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Natural Science Foundation of China","award":["32000965"],"award-info":[{"award-number":["32000965"]}]},{"name":"National Natural Science Foundation of China","award":["81801856"],"award-info":[{"award-number":["81801856"]}]},{"name":"National Natural Science Foundation of China","award":["BK20200964"],"award-info":[{"award-number":["BK20200964"]}]},{"name":"National Natural Science Foundation of China","award":["MS22022101"],"award-info":[{"award-number":["MS22022101"]}]},{"name":"Natural Science Foundation of Jiangsu Province","award":["32000965"],"award-info":[{"award-number":["32000965"]}]},{"name":"Natural Science Foundation of Jiangsu Province","award":["81801856"],"award-info":[{"award-number":["81801856"]}]},{"name":"Natural Science Foundation of Jiangsu Province","award":["BK20200964"],"award-info":[{"award-number":["BK20200964"]}]},{"name":"Natural Science Foundation of Jiangsu Province","award":["MS22022101"],"award-info":[{"award-number":["MS22022101"]}]},{"name":"Nantong Science and Technology Project","award":["32000965"],"award-info":[{"award-number":["32000965"]}]},{"name":"Nantong Science and Technology Project","award":["81801856"],"award-info":[{"award-number":["81801856"]}]},{"name":"Nantong Science and Technology Project","award":["BK20200964"],"award-info":[{"award-number":["BK20200964"]}]},{"name":"Nantong Science and Technology Project","award":["MS22022101"],"award-info":[{"award-number":["MS22022101"]}]},{"name":"Large Instruments Open Foundation of Nantong University","award":["32000965"],"award-info":[{"award-number":["32000965"]}]},{"name":"Large Instruments Open Foundation of Nantong University","award":["81801856"],"award-info":[{"award-number":["81801856"]}]},{"name":"Large Instruments Open Foundation of Nantong University","award":["BK20200964"],"award-info":[{"award-number":["BK20200964"]}]},{"name":"Large Instruments Open Foundation of Nantong University","award":["MS22022101"],"award-info":[{"award-number":["MS22022101"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>In this study, a hydrogel composite wound dressing with antibacterial and self-healing ability was prepared using cysteine-modified carboxymethyl chitosan, sodium oxidized alginate, and but-3-yn-2-one base on Schiff base and thiol-alkynone double cross-links. The structure and properties of the hydrogel were characterized by scanning electron microscope, Fourier-transform infrared, and rheological test, followed by antibacterial and in vivo biocompatibility tests. The results showed that the hydrogel exhibited good self-healing, mechanical properties, good antibacterial effect, and in vivo biocompatibility, and can inhibit inflammation and promote skin tissue regeneration in mice. This novel self-healing hydrogel dressing has a broad application prospect in skin tissue engineering.<\/jats:p>","DOI":"10.3390\/ijms232213817","type":"journal-article","created":{"date-parts":[[2022,11,10]],"date-time":"2022-11-10T01:48:08Z","timestamp":1668044888000},"page":"13817","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Dynamic Double Cross-Linked Self-Healing Polysaccharide Hydrogel Wound Dressing Based on Schiff Base and Thiol-Alkynone Reactions"],"prefix":"10.3390","volume":"23","author":[{"given":"Zhuojun","family":"Zhang","sequence":"first","affiliation":[{"name":"School of Life Sciences, Nantong University, Nantong 226019, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiasen","family":"Bu","sequence":"additional","affiliation":[{"name":"School of Life Sciences, Nantong University, Nantong 226019, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Biyun","family":"Li","sequence":"additional","affiliation":[{"name":"School of Life Sciences, Nantong University, Nantong 226019, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongyun","family":"Xuan","sequence":"additional","affiliation":[{"name":"School of Life Sciences, Nantong University, Nantong 226019, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4664-4787","authenticated-orcid":false,"given":"Yan","family":"Jin","sequence":"additional","affiliation":[{"name":"School of Life Sciences, Nantong University, Nantong 226019, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8657-984X","authenticated-orcid":false,"given":"Huihua","family":"Yuan","sequence":"additional","affiliation":[{"name":"School of Life Sciences, Nantong University, Nantong 226019, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,11,10]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Vig, K., Chaudhari, A., Tripathi, S., Dixit, S., Sahu, R., Pillai, S., Dennis, V.A., and Singh, S.R. (2017). Advances in skin regeneration using tissue engineering. Int. J. Mol. Sci., 18.","DOI":"10.3390\/ijms18040789"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1177\/0022034509359125","article-title":"Factors affecting wound healing","volume":"89","author":"Guo","year":"2010","journal-title":"J. Dent. Res."},{"key":"ref_3","first-page":"190436","article-title":"Advances in wound healing: A review of current wound healing products","volume":"2012","author":"Murphy","year":"2012","journal-title":"Plast. Surg. Int."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"5065","DOI":"10.1016\/j.biomaterials.2014.02.049","article-title":"Tissue-engineered dermo-epidermal skin grafts prevascularized with adipose-derived cells","volume":"35","author":"Klar","year":"2014","journal-title":"Biomaterials"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"573","DOI":"10.1016\/S0094-1298(03)00075-0","article-title":"Skin tissue engineering","volume":"30","author":"Bannasch","year":"2003","journal-title":"Clin. Plast. Surg."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"230","DOI":"10.1089\/ten.teb.2019.0281","article-title":"Design considerations for hydrogel wound dressings: Strategic and molecular advances","volume":"26","author":"Walboomers","year":"2020","journal-title":"Tissue Eng. B Rev."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1002\/adhm.202200494","article-title":"Hydrogel combined with phototherapy in wound healing","volume":"11","author":"Xu","year":"2022","journal-title":"Adv. Healthc. Mater."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"P1","DOI":"10.1088\/1748-3182\/2\/1\/P01","article-title":"Self-healing polymer composites: Mimicking nature to enhance performance","volume":"2","author":"Trask","year":"2007","journal-title":"Bioinspir. Biomim."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Xu, J., Liu, Y., and Hsu, S.H. (2019). Hydrogels based on Schiff base linkages for biomedical applications. Molecules, 24.","DOI":"10.3390\/molecules24163005"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"3971","DOI":"10.1039\/C5SM00587F","article-title":"A dynamic and self-crosslinked polysaccharide hydrogel with autonomous self-healing ability","volume":"11","author":"Ding","year":"2015","journal-title":"Soft Matter"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"2222","DOI":"10.1016\/j.cej.2017.11.103","article-title":"Polysaccharide-templated preparation of mechanically-tough, conductive and self-healing hydrogels","volume":"334","author":"Liu","year":"2018","journal-title":"Chem. Eng. J."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1016\/j.jconrel.2007.01.004","article-title":"Polysaccharide hydrogels for modified release formulations","volume":"119","author":"Coviello","year":"2007","journal-title":"J. Control. Release"},{"key":"ref_13","first-page":"1","article-title":"Chitosan based hydrogels: Characteristics and pharmaceutical applications","volume":"10","author":"Ahmadi","year":"2015","journal-title":"Res. Pharm. Sci."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"2618","DOI":"10.1021\/am300292v","article-title":"Flexible and microporous chitosan hydrogel\/Nano ZnO composite bandages for wound dressing: In vitro and in vivo evaluation","volume":"4","author":"Kumar","year":"2012","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"112264","DOI":"10.1016\/j.msec.2021.112264","article-title":"Injectable nanofiber-polysaccharide self-healing hydrogels for wound healing","volume":"128","author":"Xuan","year":"2021","journal-title":"Mater. Sci. Eng. C Mater. Biol. Appl."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"117870","DOI":"10.1016\/j.carbpol.2021.117870","article-title":"A double-network polysaccharide-based composite hydrogel for skin wound healing","volume":"261","author":"He","year":"2021","journal-title":"Carbohydr. Polym."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"110669","DOI":"10.1016\/j.matdes.2022.110669","article-title":"A spray-filming, tissue-adhesive, and bioactive polysaccharide self-healing hydrogel for skin regeneration","volume":"217","author":"Wu","year":"2022","journal-title":"Mater. Des."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"E143","DOI":"10.1002\/app.36548","article-title":"Correlation of the structure, properties, and antimicrobial activity of a soluble thiolated chitosan derivative","volume":"125","author":"Han","year":"2012","journal-title":"J. Appl. Polym. Sci."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"526","DOI":"10.1007\/s12257-013-0498-7","article-title":"Covalent modification of cellulosic-based textiles: A new strategy to obtain antimicrobial properties","volume":"19","author":"Nogueira","year":"2014","journal-title":"Biotechnol. Bioproc. Eng."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"776","DOI":"10.1021\/acsmacrolett.0c00241","article-title":"Self-healing injectable polymer hydrogel via dynamic thiol-alkynone double addition cross-links","volume":"9","author":"Fan","year":"2020","journal-title":"ACS Macro Lett."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"3941","DOI":"10.1016\/j.biomaterials.2004.10.005","article-title":"Self-cross-linking biopolymers as injectable in situ forming biodegradable scaffolds","volume":"26","author":"Balakrishnan","year":"2005","journal-title":"Biomaterials"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"596","DOI":"10.1021\/acs.biomac.7b01679","article-title":"Injectable self-healing zwitterionic hydrogels based on dynamic benzoxaborole-sugar interactions with tunable mechanical properties","volume":"19","author":"Chen","year":"2018","journal-title":"Biomacromolecules"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"111146","DOI":"10.1016\/j.lwt.2021.111146","article-title":"Detoxification of patulin from juice simulator and apple juice via cross-linked Se-chitosan\/L-cysteine nanoparticles","volume":"143","author":"Paimard","year":"2021","journal-title":"LWT Food Sci. Technol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"597","DOI":"10.1016\/j.carbpol.2007.01.016","article-title":"Preparation and characterization of self-aggregated nanoparticles of cholesterol-modified O-carboxymethyl chitosan conjugates","volume":"69","author":"Yinsong","year":"2007","journal-title":"Carbohydr. Polym."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"502058","DOI":"10.1155\/2015\/502058","article-title":"Synthesis and characterization of new thiolated chitosan nanoparticles obtained by ionic gelation method","volume":"2015","author":"Esquivel","year":"2015","journal-title":"Int. J. Polym. Sci."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"336","DOI":"10.1080\/00914037.2020.1833011","article-title":"Design and in vitro evaluation of pH-sensitive crosslinked chitosan-grafted acrylic acid copolymer (CS-co-AA) for targeted drug delivery","volume":"71","author":"Ijaz","year":"2022","journal-title":"Int. J. Polym. Mater. Polym. Biomater."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1889","DOI":"10.1021\/acs.biomac.8b01836","article-title":"Biocompatible lipopeptide-based antibacterial hydrogel","volume":"20","author":"Adak","year":"2019","journal-title":"Biomacromolecules"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"2879","DOI":"10.1021\/acsabm.9b00266","article-title":"Development of responsive chitosan-genipin hydrogels for the treatment of wounds","volume":"2","author":"Heimbuck","year":"2019","journal-title":"ACS Appl. Bio Mater."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"118513","DOI":"10.1016\/j.carbpol.2021.118513","article-title":"A synergy between dopamine and electrostatically bound bactericide in a poly (vinyl alcohol) hybrid hydrogel for treating infected wounds","volume":"272","author":"Lei","year":"2021","journal-title":"Carbohydr. Polym."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"115671","DOI":"10.1016\/j.carbpol.2019.115671","article-title":"A novel and green cellulose-based Schiff base-Cu (II) complex and its excellent antibacterial activity","volume":"230","author":"Xu","year":"2020","journal-title":"Carbohydr. Polym."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"802","DOI":"10.1016\/j.ijbiomac.2018.11.229","article-title":"Synthesis, characterization and antimicrobial activity of Schiff bases from chitosan and salicylaldehyde\/TiO2 nanocomposite membrane","volume":"124","author":"Montaser","year":"2019","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"15505","DOI":"10.1016\/j.ceramint.2019.05.054","article-title":"Antimicrobial property and photocatalytic antibacterial mechanism of the TiO2-doped SiO2 hybrid materials under ultraviolet-light irradiation and visible-light irradiation","volume":"45","author":"Chen","year":"2019","journal-title":"Ceram. Int."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"296","DOI":"10.1016\/j.carbpol.2006.05.025","article-title":"Oxidation of sodium alginate and characterization of the oxidized derivatives","volume":"67","author":"Gomez","year":"2007","journal-title":"Carbohydr. Polym."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"376","DOI":"10.1016\/j.eurpolymj.2019.08.009","article-title":"In situ cross\u2013linked chitosan hydrogels via Michael addition reaction based on water\u2013soluble thiol\u2013maleimide precursors","volume":"119","author":"Guaresti","year":"2019","journal-title":"Eur. Polym. J."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/23\/22\/13817\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T01:13:34Z","timestamp":1760145214000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/23\/22\/13817"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,11,10]]},"references-count":34,"journal-issue":{"issue":"22","published-online":{"date-parts":[[2022,11]]}},"alternative-id":["ijms232213817"],"URL":"https:\/\/doi.org\/10.3390\/ijms232213817","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,11,10]]}}}