{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,11]],"date-time":"2026-05-11T12:41:17Z","timestamp":1778503277816,"version":"3.51.4"},"reference-count":62,"publisher":"MDPI AG","issue":"22","license":[{"start":{"date-parts":[[2022,11,10]],"date-time":"2022-11-10T00:00:00Z","timestamp":1668038400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"the Ministry of Science and Technology","award":["107-2314-B-303-012-"],"award-info":[{"award-number":["107-2314-B-303-012-"]}]},{"name":"the Ministry of Science and Technology","award":["TCRD 108-4"],"award-info":[{"award-number":["TCRD 108-4"]}]},{"name":"the Ministry of Science and Technology","award":["TCRD 111-057"],"award-info":[{"award-number":["TCRD 111-057"]}]},{"name":"the Ministry of Science and Technology","award":["TCMF-EP-108-02"],"award-info":[{"award-number":["TCMF-EP-108-02"]}]},{"name":"the Ministry of Science and Technology","award":["TCMF-EP 111-01"],"award-info":[{"award-number":["TCMF-EP 111-01"]}]},{"name":"Hualien Tzu Chi Hospital","award":["107-2314-B-303-012-"],"award-info":[{"award-number":["107-2314-B-303-012-"]}]},{"name":"Hualien Tzu Chi Hospital","award":["TCRD 108-4"],"award-info":[{"award-number":["TCRD 108-4"]}]},{"name":"Hualien Tzu Chi Hospital","award":["TCRD 111-057"],"award-info":[{"award-number":["TCRD 111-057"]}]},{"name":"Hualien Tzu Chi Hospital","award":["TCMF-EP-108-02"],"award-info":[{"award-number":["TCMF-EP-108-02"]}]},{"name":"Hualien Tzu Chi Hospital","award":["TCMF-EP 111-01"],"award-info":[{"award-number":["TCMF-EP 111-01"]}]},{"name":"the Buddhist Tzu Chi Medical Foundation","award":["107-2314-B-303-012-"],"award-info":[{"award-number":["107-2314-B-303-012-"]}]},{"name":"the Buddhist Tzu Chi Medical Foundation","award":["TCRD 108-4"],"award-info":[{"award-number":["TCRD 108-4"]}]},{"name":"the Buddhist Tzu Chi Medical Foundation","award":["TCRD 111-057"],"award-info":[{"award-number":["TCRD 111-057"]}]},{"name":"the Buddhist Tzu Chi Medical Foundation","award":["TCMF-EP-108-02"],"award-info":[{"award-number":["TCMF-EP-108-02"]}]},{"name":"the Buddhist Tzu Chi Medical Foundation","award":["TCMF-EP 111-01"],"award-info":[{"award-number":["TCMF-EP 111-01"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Ovarian cancer is one of the most lethal gynecological cancers, and 80% are high-grade serous carcinomas (HGSOC). Despite advances in chemotherapy and the development of targeted therapies, the survival rate of HGSOC has only moderately improved. Therefore, a cell model that reflects the pathogenesis and clinical characteristics of this disease is urgently needed. We previously developed a human fallopian tube epithelial cell line (FE25) with p53 and Rb deficiencies. After long-term culture in vitro, cells at high-passage numbers showed spontaneous transformation (FE25L). This study aimed to compare FE25 cells cultured in vitro for low (passage 16\u201331) and high passages (passage 116\u2013139) to determine whether these cells can serve as an ideal cell model of HGSOC. Compared to the cells at low passage, FE25L cells showed increased cell proliferation, clonogenicity, polyploidy, aneuploidy, cell migration, and invasion. They also showed more resistance to chemotherapy and the ability to grow tumors in xenografts. RNA-seq data also showed upregulation of hypoxia, epithelial-mesenchymal transition (EMT), and the NF-\u03baB pathway in FE25L compared to FE25 cells. qRT-PCR confirmed the upregulation of EMT, cytokines, NF-\u03baB, c-Myc, and the Wnt\/\u03b2-catenin pathway. Cross-platform comparability found that FE25L cells could be grouped with the other most likely HGSOC lines, such as TYKNU and COV362. In conclusion, FE25L cells showed more aggressive malignant behavior than FE25 cells and hence might serve as a more suitable model for HGSOC research.<\/jats:p>","DOI":"10.3390\/ijms232213843","type":"journal-article","created":{"date-parts":[[2022,11,10]],"date-time":"2022-11-10T19:16:23Z","timestamp":1668107783000},"page":"13843","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Spontaneous Transformation of a p53 and Rb-Defective Human Fallopian Tube Epithelial Cell Line after Long Passage with Features of High-Grade Serous Carcinoma"],"prefix":"10.3390","volume":"23","author":[{"given":"Yu-Hsun","family":"Chang","sequence":"first","affiliation":[{"name":"Department of Pediatrics, Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Tzu Chi University, Hualien 97005, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tang-Yuan","family":"Chu","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Tzu Chi University, Hualien 97005, Taiwan"},{"name":"Institute of Medical Sciences, Collagen of Medicine, Tzu Chi University, Hualien 97005, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5105-068X","authenticated-orcid":false,"given":"Dah-Ching","family":"Ding","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Tzu Chi University, Hualien 97005, Taiwan"},{"name":"Institute of Medical Sciences, Collagen of Medicine, Tzu Chi University, Hualien 97005, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,11,10]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"9","DOI":"10.20892\/j.issn.2095-3941.2016.0084","article-title":"Epidemiology of Ovarian Cancer: A Review","volume":"14","author":"Reid","year":"2017","journal-title":"Cancer Biol. Med."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Zhang, Y., Luo, G., Li, M., Guo, P., Xiao, Y., Ji, H., and Hao, Y. (2019). Global patterns and trends in ovarian cancer incidence: Age, period and birth cohort analysis. BMC Cancer, 19.","DOI":"10.1186\/s12885-019-6139-6"},{"key":"ref_3","first-page":"339","article-title":"Using PARP Inhibitors in Advanced Ovarian Cancer","volume":"32","year":"2018","journal-title":"Oncology"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"733","DOI":"10.1016\/j.ajpath.2015.11.011","article-title":"The Dualistic Model of Ovarian Carcinogenesis: Revisited, Revised, and Expanded","volume":"186","author":"Kurman","year":"2016","journal-title":"Am. J. Pathol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"433","DOI":"10.1097\/PAS.0b013e3181cf3d79","article-title":"The Origin and Pathogenesis of Epithelial Ovarian Cancer: A Proposed Unifying Theory","volume":"34","author":"Kurman","year":"2010","journal-title":"Am. J. Surg. Pathol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"650","DOI":"10.21037\/tcr.2016.08.40","article-title":"TP53 mutations in epithelial ovarian cancer","volume":"5","author":"Zhang","year":"2016","journal-title":"Transl. Cancer Res."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"200","DOI":"10.1101\/gr.161026.113","article-title":"Genomic and transcriptomic plasticity in treatment-na\u00efve ovarian cancer","volume":"24","author":"Hoogstraat","year":"2014","journal-title":"Genome Res."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"19489","DOI":"10.1073\/pnas.1314302110","article-title":"Synthetic lethality between CCNE1 amplification and loss of BRCA1","volume":"110","author":"Etemadmoghadam","year":"2013","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"a026104","DOI":"10.1101\/cshperspect.a026104","article-title":"The Cell-Cycle Arrest and Apoptotic Functions of p53 in Tumor Initiation and Progression","volume":"6","author":"Chen","year":"2016","journal-title":"Cold Spring Harb. Perspect. Med."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"5284","DOI":"10.1200\/JCO.2008.18.1107","article-title":"New Insights Into the Pathogenesis of Serous Ovarian Cancer and Its Clinical Impact","volume":"26","author":"Levanon","year":"2008","journal-title":"J. Clin. Oncol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1097\/GCO.0b013e328011a21f","article-title":"The distal fallopian tube: A new model for pelvic serous carcinogenesis","volume":"19","author":"Crum","year":"2007","journal-title":"Curr. Opin. Obstet. Gynecol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"597","DOI":"10.1016\/j.ebiom.2019.01.061","article-title":"IGF-axis confers transformation and regeneration of fallopian tube fimbria epithelium upon ovulation","volume":"41","author":"Hsu","year":"2019","journal-title":"eBioMedicine"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"7547","DOI":"10.1073\/pnas.1017300108","article-title":"Modeling high-grade serous ovarian carcinogenesis from the fallopian tube","volume":"108","author":"Karst","year":"2011","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1141","DOI":"10.1158\/0008-5472.CAN-13-2247","article-title":"Cyclin E1 Deregulation Occurs Early in Secretory Cell Transformation to Promote Formation of Fallopian Tube\u2013Derived High-Grade Serous Ovarian Cancers","volume":"74","author":"Karst","year":"2014","journal-title":"Cancer Res."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"e2114162","DOI":"10.1001\/jamanetworkopen.2021.14162","article-title":"Association of Copy Number Variation Signature and Survival in Patients With Serous Ovarian Cancer","volume":"4","author":"Graf","year":"2021","journal-title":"JAMA Netw. Open"},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"The Cancer Genome Atlas Research Network (2011). Integrated Genomic Analyses of Ovarian Carcinoma. Nature, 474, 609\u2013615.","DOI":"10.1038\/nature10166"},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Voutsadakis, I.A. (2020). 8p11.23 Amplification in Breast Cancer: Molecular Characteristics, Prognosis and Targeted Therapy. J. Clin. Med., 9.","DOI":"10.3390\/jcm9103079"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1027","DOI":"10.3892\/ol.2012.623","article-title":"A chronic myeloid leukemia case with a unique variant Philadelphia translocation: T(9;22;21)(q34;q11;p12)","volume":"3","author":"Wafa","year":"2012","journal-title":"Oncol. Lett."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"15545","DOI":"10.1073\/pnas.0506580102","article-title":"Gene set enrichment analysis: A knowledge-based approach for interpreting genome-wide expression profiles","volume":"102","author":"Subramanian","year":"2005","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Sohn, M.-H., Kim, S., Shin, J.-Y., Kim, H., Chung, H., Kim, J.-W., Lee, M., and Seo, J.-S. (2021). Classification of High-Grade Serous Ovarian Carcinoma by Epithelial-to-Mesenchymal Transition Signature and Homologous Recombination Repair Genes. Genes, 12.","DOI":"10.3390\/genes12071103"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"24","DOI":"10.3389\/fonc.2017.00024","article-title":"The Unique Molecular and Cellular Microenvironment of Ovarian Cancer","volume":"7","author":"Worzfeld","year":"2017","journal-title":"Front. Oncol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"213","DOI":"10.3892\/etm.2011.194","article-title":"Cyclin D1 predicts the prognosis of advanced serous ovarian cancer","volume":"2","author":"Hashimoto","year":"2011","journal-title":"Exp. Ther. Med."},{"key":"ref_23","first-page":"68","article-title":"Targeting the PI3K\/AKT\/mTOR\/NF\u03baB Axis in Ovarian Cancer","volume":"2","author":"Ghoneum","year":"2020","journal-title":"J. Cell. Immunol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"53","DOI":"10.3389\/fonc.2014.00053","article-title":"A New Spontaneously Transformed Syngeneic Model of High-Grade Serous Ovarian Cancer with a Tumor-Initiating Cell Population","volume":"4","author":"McCloskey","year":"2014","journal-title":"Front. Oncol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"e39030","DOI":"10.7554\/eLife.39030","article-title":"Targeting MYC Dependency in Ovarian Cancer through Inhibition of CDK7 and CDK12\/13","volume":"7","author":"Zeng","year":"2018","journal-title":"eLife"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1186\/s13048-019-0596-z","article-title":"Wnt\/\u03b2-catenin signalling in ovarian cancer: Insights into its hyperactivation and function in tumorigenesis","volume":"12","author":"Nguyen","year":"2019","journal-title":"J. Ovarian Res."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"2126","DOI":"10.1038\/ncomms3126","article-title":"Evaluating cell lines as tumour models by comparison of genomic profiles","volume":"4","author":"Domcke","year":"2013","journal-title":"Nat. Commun."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1419","DOI":"10.1093\/carcin\/bgv132","article-title":"Mutagenic, surviving and tumorigenic effects of follicular fluid in the context of p53 loss: Initiation of fimbria carcinogenesis","volume":"36","author":"Huang","year":"2015","journal-title":"Carcinogenesis"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"484","DOI":"10.1002\/path.4807","article-title":"Haemoglobin in pelvic fluid rescues Fallopian tube epithelial cells from reactive oxygen species stress and apoptosis","volume":"240","author":"Huang","year":"2016","journal-title":"J. Pathol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"751","DOI":"10.1016\/j.ccr.2013.10.013","article-title":"Transformation of the Fallopian Tube Secretory Epithelium Leads to High-Grade Serous Ovarian Cancer in Brca;Tp53;Pten Models","volume":"24","author":"Perets","year":"2013","journal-title":"Cancer Cell"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1123","DOI":"10.1016\/j.neo.2021.09.006","article-title":"Ovulation sources coagulation protease cascade and hepatocyte growth factor to support physiological growth and malignant transformation","volume":"23","author":"Huang","year":"2021","journal-title":"Neoplasia"},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Hsu, C.-F., Chen, P.-C., Seenan, V., Ding, D.-C., and Chu, T.-Y. (2021). Ovulatory Follicular Fluid Facilitates the Full Transformation Process for the Development of High-Grade Serous Carcinoma. Cancers, 13.","DOI":"10.3390\/cancers13030468"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"e13029","DOI":"10.1111\/cpr.13029","article-title":"Cellular models of development of ovarian high-grade serous carcinoma: A review of cell of origin and mechanisms of carcinogenesis","volume":"54","author":"Mei","year":"2021","journal-title":"Cell Prolif."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"585","DOI":"10.1093\/carcin\/21.4.585","article-title":"Development of a syngeneic mouse model for events related to ovarian cancer","volume":"21","author":"Roby","year":"2000","journal-title":"Carcinogenesis"},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Quartuccio, S.M., Lantvit, D.D., Bosland, M.C., and Burdette, J.E. (2013). Conditional Inactivation of p53 in Mouse Ovarian Surface Epithelium Does Not Alter MIS Driven Smad2-Dominant Negative Epithelium-Lined Inclusion Cysts or Teratomas. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0065067"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"268","DOI":"10.1200\/JCO.2005.01.5180","article-title":"EZH2 expression is associated with high proliferation rate and aggressive tumor subgroups in cutaneous melanoma and cancers of the endometrium, prostate, and breast","volume":"24","author":"Bachmann","year":"2006","journal-title":"J. Clin. Oncol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"5288","DOI":"10.1167\/iovs.16-19317","article-title":"Effects of Long-term Serial Passaging on the Characteristics and Properties of Cell Lines Derived From Uveal Melanoma Primary Tumors","volume":"57","author":"Mouriaux","year":"2016","journal-title":"Investig. Opthalmol. Vis. Sci."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"492","DOI":"10.1038\/nature11935","article-title":"Replication stress links structural and numerical cancer chromosomal instability","volume":"494","author":"Burrell","year":"2013","journal-title":"Nature"},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Vargas-Rond\u00f3n, N., Villegas, V.E., and Rond\u00f3n-Lagos, M. (2017). The Role of Chromosomal Instability in Cancer and Therapeutic Responses. Cancers, 10.","DOI":"10.3390\/cancers10010004"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"3259","DOI":"10.1200\/JCO.2007.11.3902","article-title":"Sporadic Epithelial Ovarian Cancer: Clinical Relevance of BRCA1 Inhibition in the DNA Damage and Repair Pathway","volume":"26","author":"Weberpals","year":"2008","journal-title":"J. Clin. Oncol."},{"key":"ref_41","first-page":"351","article-title":"Frequent deletion of chromosome 19 and a rare rearrangement of 19p13.3 involving the insulin receptor gene in human ovarian cancer","volume":"11","author":"Amfo","year":"1995","journal-title":"Oncogene"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"14423","DOI":"10.1038\/ncomms14423","article-title":"Haploinsufficiency networks identify targetable patterns of allelic deficiency in low mutation ovarian cancer","volume":"8","author":"Delaney","year":"2017","journal-title":"Nat. Commun."},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Schwarz, R., Ng, C.K.Y., Cooke, S.L., Newmann, S., Temple, J., Piskorz, A.M., Gale, D., Sayal, K., Murtaza, M., and Baldwin, P.J. (2015). Spatial and Temporal Heterogeneity in High-Grade Serous Ovarian Cancer: A Phylogenetic Analysis. PLoS Med., 12.","DOI":"10.1371\/journal.pmed.1001789"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"707","DOI":"10.1126\/science.2470152","article-title":"Studies of the HER-2\/neu Proto-Oncogene in Human Breast and Ovarian Cancer","volume":"244","author":"Slamon","year":"1989","journal-title":"Science"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1309\/N6X5MB24BP42KP20","article-title":"Analysis of Gene Amplification and Prognostic Markers in Ovarian Cancer Using Comparative Genomic Hybridization for Microarrays and Immunohistochemical Analysis for Tissue Microarrays","volume":"126","author":"Mayr","year":"2006","journal-title":"Am. J. Clin. Pathol."},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Devaja, O., and Papadopoulos, A. (2018). Genomic Copy Number Alterations in Serous Ovarian Cancer. Ovarian Cancer\u2014From Pathogenesis to Treatment, IntechOpen.","DOI":"10.5772\/66599"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"8591","DOI":"10.1158\/0008-5472.CAN-05-0239","article-title":"Caspase-1\u03b1 Is Down-regulated in Human Ovarian Cancer Cells and the Overexpression of Caspase-1\u03b1 Induces Apoptosis","volume":"65","author":"Feng","year":"2005","journal-title":"Cancer Res."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1186\/s13048-018-0388-x","article-title":"Investigation of hypoxia networks in ovarian cancer via bioinformatics analysis","volume":"11","author":"Zhang","year":"2018","journal-title":"J. Ovarian Res."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1038\/s41580-020-0237-9","article-title":"Guidelines and definitions for research on epithelial\u2013mesenchymal transition","volume":"21","author":"Yang","year":"2020","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1016\/j.canlet.2014.05.022","article-title":"Epithelial\u2013mesenchymal transition-associated miRNAs in ovarian carcinoma, with highlight on the miR-200 family: Prognostic value and prospective role in ovarian cancer therapeutics","volume":"351","author":"Koutsaki","year":"2014","journal-title":"Cancer Lett."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1186\/1757-2215-7-76","article-title":"The EMT (epithelial-mesenchymal-transition)-related protein expression indicates the metastatic status and prognosis in patients with ovarian cancer","volume":"7","author":"Takai","year":"2014","journal-title":"J. Ovarian Res."},{"key":"ref_52","first-page":"566","article-title":"Profiling of cytokines in human epithelial ovarian cancer ascites","volume":"2","author":"Matte","year":"2012","journal-title":"Am. J. Cancer Res."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"1815","DOI":"10.7150\/jca.16116","article-title":"Interleukin-6 from Ovarian Mesenchymal Stem Cells Promotes Proliferation, Sphere and Colony Formation and Tumorigenesis of an Ovarian Cancer Cell Line SKOV3","volume":"7","author":"Ding","year":"2016","journal-title":"J. Cancer"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"5927","DOI":"10.1038\/onc.2017.185","article-title":"\u03b2-catenin downregulates Dicer to promote ovarian cancer metastasis","volume":"36","author":"To","year":"2017","journal-title":"Oncogene"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"214","DOI":"10.1016\/j.yexcr.2018.08.013","article-title":"BRMS1L suppresses ovarian cancer metastasis via inhibition of the \u03b2-catenin-wnt pathway","volume":"371","author":"Cao","year":"2018","journal-title":"Exp. Cell Res."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"1356508","DOI":"10.1155\/2019\/1356508","article-title":"Molecular Regulation of Cancer Cell Migration, Invasion, and Metastasis","volume":"2019","author":"Tahtamouni","year":"2019","journal-title":"Anal. Cell. Pathol."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1186\/1757-2215-7-45","article-title":"Resistance to cisplatin and paclitaxel does not affect the sensitivity of human ovarian cancer cells to antiprogestin-induced cytotoxicity","volume":"7","author":"Hapon","year":"2014","journal-title":"J. Ovarian Res."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"1179299X19860815","DOI":"10.1177\/1179299X19860815","article-title":"Chemotherapy Resistance in Advanced Ovarian Cancer Patients","volume":"11","author":"Pokhriyal","year":"2019","journal-title":"Biomark. Cancer"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"148","DOI":"10.3389\/fonc.2013.00148","article-title":"Cancer Karyotypes: Survival of the Fittest","volume":"3","author":"Nicholson","year":"2013","journal-title":"Front. Oncol."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1177\/1758834010361333","article-title":"Review: Practical considerations in ovarian cancer chemotherapy","volume":"2","author":"Cristea","year":"2010","journal-title":"Ther. Adv. Med. Oncol."},{"key":"ref_61","doi-asserted-by":"crossref","unstructured":"Talevich, E., Shain, A.H., Botton, T., and Bastian, B.C. (2016). CNVkit: Genome-Wide Copy Number Detection and Visualization from Targeted DNA Sequencing. PLoS Comput. Biol., 12.","DOI":"10.1371\/journal.pcbi.1004873"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.jim.2018.03.004","article-title":"A new background subtraction method for Western blot densitometry band quantification through image analysis software","volume":"457","author":"Dotor","year":"2018","journal-title":"J. Immunol. Methods"}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/23\/22\/13843\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T01:13:52Z","timestamp":1760145232000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/23\/22\/13843"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,11,10]]},"references-count":62,"journal-issue":{"issue":"22","published-online":{"date-parts":[[2022,11]]}},"alternative-id":["ijms232213843"],"URL":"https:\/\/doi.org\/10.3390\/ijms232213843","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,11,10]]}}}