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Aptamers were obtained via SELEX and next-generation sequencing, followed by colorimetric and fluorescent characterizations. An APTA-RT-PCR assay was developed, optimized, and tested against the viral RNAs in 108 serum samples. After selection, sequence APTAZC10 was designed as a bifunctional molecular beacon (APTAZC10-MB), exhibiting affinity for the viral targets. APTA-RT-PCR was able to detect Dengue and Zika RNA in 43% and 8% of samples, respectively. Our results indicate that APTAZC10-MB and APTA-RT-PCR will be useful to improve the detection of Dengue and Zika viruses in a fast molecular assay for the improvement of infectious disease surveillance.<\/jats:p>","DOI":"10.3390\/ijms232213866","type":"journal-article","created":{"date-parts":[[2022,11,10]],"date-time":"2022-11-10T19:16:23Z","timestamp":1668107783000},"page":"13866","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Development of a Molecular Aptamer Beacon Applied to Magnetic-Assisted RNA Extraction for Detection of Dengue and Zika Viruses Using Clinical Samples"],"prefix":"10.3390","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1794-5660","authenticated-orcid":false,"given":"Amanda Gabrielle","family":"da Silva","sequence":"first","affiliation":[{"name":"Institute of Physics, Postgraduate Program in Exact and Technological Sciences, Universidade Federal de Catal\u00e3o, Catal\u00e3o 75704-020, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1803-4861","authenticated-orcid":false,"given":"Luiz Ricardo","family":"Goulart","sequence":"additional","affiliation":[{"name":"Nanobiotechnology Laboratory, Institute of Biotechnology, Universidade Federal de Uberl\u00e2ndia, Uberl\u00e2ndia 38402-022, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8995-6783","authenticated-orcid":false,"given":"Philipp","family":"L\u00f6ffler","sequence":"additional","affiliation":[{"name":"Department of Physics, Chemistry and Pharmacy, University of Southern Denmark, Campusvej 55, 5230 Odense, Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christian","family":"Code","sequence":"additional","affiliation":[{"name":"Dianox ApS, Fruebjergvej 3, 2100 K\u00f8benhavn, Denmark"},{"name":"PhyLife Physical Life Sciences, Department of Physics, Chemistry and Pharmacy, University of Southern Denmark, Campusvej 55, 5230 Odense, Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2299-2114","authenticated-orcid":false,"given":"Adriana Freitas","family":"Neves","sequence":"additional","affiliation":[{"name":"Institute of Biotechnology, Molecular Biology Laboratory, Universidade Federal de Catal\u00e3o, Catal\u00e3o 75704-020, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,11,10]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"302","DOI":"10.1016\/j.medmal.2014.04.008","article-title":"Current Zika virus epidemiology and recent epidemics","volume":"44","author":"Ioos","year":"2014","journal-title":"M\u00e9d. 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