{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,18]],"date-time":"2026-01-18T09:52:46Z","timestamp":1768729966171,"version":"3.49.0"},"reference-count":81,"publisher":"MDPI AG","issue":"22","license":[{"start":{"date-parts":[[2022,11,12]],"date-time":"2022-11-12T00:00:00Z","timestamp":1668211200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Natural Science Foundation of China Grant","award":["31570674"],"award-info":[{"award-number":["31570674"]}]},{"name":"National Natural Science Foundation of China Grant","award":["324-1122yb061"],"award-info":[{"award-number":["324-1122yb061"]}]},{"name":"National Natural Science Foundation of China Grant","award":["CXZX2020093A"],"award-info":[{"award-number":["CXZX2020093A"]}]},{"name":"National Natural Science Foundation of China Grant","award":["72202200205"],"award-info":[{"award-number":["72202200205"]}]},{"name":"Scientific Research Foundation of Graduate School of Fujian Agriculture and Forestry University","award":["31570674"],"award-info":[{"award-number":["31570674"]}]},{"name":"Scientific Research Foundation of Graduate School of Fujian Agriculture and Forestry University","award":["324-1122yb061"],"award-info":[{"award-number":["324-1122yb061"]}]},{"name":"Scientific Research Foundation of Graduate School of Fujian Agriculture and Forestry University","award":["CXZX2020093A"],"award-info":[{"award-number":["CXZX2020093A"]}]},{"name":"Scientific Research Foundation of Graduate School of Fujian Agriculture and Forestry University","award":["72202200205"],"award-info":[{"award-number":["72202200205"]}]},{"name":"Innovation Fund for Science and Technology Project from Fujian Agriculture and Forestry University","award":["31570674"],"award-info":[{"award-number":["31570674"]}]},{"name":"Innovation Fund for Science and Technology Project from Fujian Agriculture and Forestry University","award":["324-1122yb061"],"award-info":[{"award-number":["324-1122yb061"]}]},{"name":"Innovation Fund for Science and Technology Project from Fujian Agriculture and Forestry University","award":["CXZX2020093A"],"award-info":[{"award-number":["CXZX2020093A"]}]},{"name":"Innovation Fund for Science and Technology Project from Fujian Agriculture and Forestry University","award":["72202200205"],"award-info":[{"award-number":["72202200205"]}]},{"name":"Forestry Peak Discipline Construction Project of Fujian Agriculture and Forestry University","award":["31570674"],"award-info":[{"award-number":["31570674"]}]},{"name":"Forestry Peak Discipline Construction Project of Fujian Agriculture and Forestry University","award":["324-1122yb061"],"award-info":[{"award-number":["324-1122yb061"]}]},{"name":"Forestry Peak Discipline Construction Project of Fujian Agriculture and Forestry University","award":["CXZX2020093A"],"award-info":[{"award-number":["CXZX2020093A"]}]},{"name":"Forestry Peak Discipline Construction Project of Fujian Agriculture and Forestry University","award":["72202200205"],"award-info":[{"award-number":["72202200205"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Cunninghamia lanceolata (C. lanceolata) belongs to Gymnospermae, which are fast-growing and have desirable wood properties. However, C. lanceolata\u2019s stress resistance is little understood. To unravel the physiological and molecular regulation mechanisms under environmental stresses in the typical gymnosperm species of C. lanceolata, three-year-old plants were exposed to simulated drought stress (polyethylene glycol 8000), salicylic acid, and cold treatment at 4 \u00b0C for 8 h, 32 h, and 56 h, respectively. Regarding the physiological traits, we observed a decreased protein content and increased peroxidase upon salicylic acid and polyethylene glycol treatment. Superoxide dismutase activity either decreased or increased at first and then returned to normal under the stresses. Regarding the molecular regulation, we used both nanopore direct RNA sequencing and short-read sequencing to reveal a total of 5646 differentially expressed genes in response to different stresses, of which most had functions in lignin catabolism, pectin catabolism, and xylan metabolism, indicating that the development of stem-differentiating xylem was affected upon stress treatment. Finally, we identified a total of 51 AP2\/ERF, 29 NAC, and 37 WRKY transcript factors in C. lanceolata. The expression of most of the NAC TFs increased under cold stress, and the expression of most of the WRKY TFs increased under cold and SA stress. These results revealed the transcriptomics responses in C. lanceolata to short-term stresses under this study\u2019s experimental conditions and provide preliminary clues about stem-differentiating xylem changes associated with different stresses.<\/jats:p>","DOI":"10.3390\/ijms232213986","type":"journal-article","created":{"date-parts":[[2022,11,14]],"date-time":"2022-11-14T02:29:32Z","timestamp":1668392972000},"page":"13986","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Transcriptome Profiling of Stem-Differentiating Xylem in Response to Abiotic Stresses Based on Hybrid Sequencing in Cunninghamia lanceolata"],"prefix":"10.3390","volume":"23","author":[{"given":"Wentao","family":"Wei","sequence":"first","affiliation":[{"name":"College of Forestry, Fujian Agriculture and Forestry University, Fuzhou 350002, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1515-9463","authenticated-orcid":false,"given":"Huiyuan","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Forestry, Fujian Agriculture and Forestry University, Fuzhou 350002, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xuqing","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Forestry, Fujian Agriculture and Forestry University, Fuzhou 350002, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenjing","family":"Kou","sequence":"additional","affiliation":[{"name":"College of Forestry, Fujian Agriculture and Forestry University, Fuzhou 350002, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ziqi","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Forestry, Fujian Agriculture and Forestry University, Fuzhou 350002, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huihui","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Forestry, Fujian Agriculture and Forestry University, Fuzhou 350002, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yongkang","family":"Yang","sequence":"additional","affiliation":[{"name":"College of Forestry, Fujian Agriculture and Forestry University, Fuzhou 350002, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liangzhen","family":"Zhao","sequence":"additional","affiliation":[{"name":"Basic Forestry and Proteomics Research Center, College of Forestry, School of Future Technology, Fujian Agriculture and Forestry University, Fuzhou 350002, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2614-2416","authenticated-orcid":false,"given":"Hangxiao","family":"Zhang","sequence":"additional","affiliation":[{"name":"Basic Forestry and Proteomics Research Center, College of Forestry, School of Future Technology, Fujian Agriculture and Forestry University, Fuzhou 350002, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3842-1586","authenticated-orcid":false,"given":"Bo","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Forestry, Fujian Agriculture and Forestry University, Fuzhou 350002, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiangqing","family":"Ma","sequence":"additional","affiliation":[{"name":"College of Forestry, Fujian Agriculture and Forestry University, Fuzhou 350002, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3810-2411","authenticated-orcid":false,"given":"Lianfeng","family":"Gu","sequence":"additional","affiliation":[{"name":"Basic Forestry and Proteomics Research Center, College of Forestry, School of Future Technology, Fujian Agriculture and Forestry University, Fuzhou 350002, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,11,12]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1140","DOI":"10.1039\/C8NP00037A","article-title":"Dynamic metabolic solutions to the sessile life style of plants","volume":"35","author":"Knudsen","year":"2018","journal-title":"Nat. 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