{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T16:21:32Z","timestamp":1792513292948,"version":"4.5.0"},"reference-count":28,"publisher":"MDPI AG","issue":"23","license":[{"start":{"date-parts":[[2022,11,22]],"date-time":"2022-11-22T00:00:00Z","timestamp":1669075200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Universit\u00e0 di Pisa","award":["PRA_2020_32"],"award-info":[{"award-number":["PRA_2020_32"]}]},{"name":"Universit\u00e0 di Pisa","award":["JPIAMR_2018_P001"],"award-info":[{"award-number":["JPIAMR_2018_P001"]}]},{"name":"ERA-NET JPI-EC-AMR","award":["PRA_2020_32"],"award-info":[{"award-number":["PRA_2020_32"]}]},{"name":"ERA-NET JPI-EC-AMR","award":["JPIAMR_2018_P001"],"award-info":[{"award-number":["JPIAMR_2018_P001"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Staphylococcus aureus implant-associated infections are difficult to treat because of the ability of bacteria to form biofilm on medical devices. Here, the efficacy of Sb-1 to control or prevent S. aureus colonization on medical foreign bodies was investigated in a Galleria mellonella larval infection model. For colonization control assays, sterile K-wires were implanted into larva prolegs. After 2 days, larvae were infected with methicillin-resistant S. aureus ATCC 43300 and incubated at 37 \u00b0C for a further 2 days, when treatments with either daptomycin (4 mg\/kg), Sb-1 (107 PFUs) or a combination of them (3 x\/day) were started. For biofilm prevention assays, larvae were pre-treated with either vancomycin (10 mg\/kg) or Sb-1 (107 PFUs) before the S. aureus infection. In both experimental settings, K-wires were explanted for colony counting two days after treatment. In comparison to the untreated control, more than a 4 log10 CFU and 1 log10 CFU reduction was observed on K-wires recovered from larvae treated with the Sb-1\/daptomycin combination and with their singular administration, respectively. Moreover, pre-infection treatment with Sb-1 was found to prevent K-wire colonization, similarly to vancomycin. Taken together, the obtained results demonstrated the strong potential of the Sb-1 antibiotic combinatory administration or the Sb-1 pretreatment to control or prevent S. aureus-associated implant infections.<\/jats:p>","DOI":"10.3390\/ijms232314514","type":"journal-article","created":{"date-parts":[[2022,11,22]],"date-time":"2022-11-22T08:25:28Z","timestamp":1669105528000},"page":"14514","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Phage-Based Control of Methicillin Resistant Staphylococcus aureus in a Galleria mellonella Model of Implant-Associated Infection"],"prefix":"10.3390","volume":"23","author":[{"given":"Alessandro","family":"Materazzi","sequence":"first","affiliation":[{"name":"Department of Biology, University of Pisa, 56127 Pisa, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daria","family":"Bottai","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Pisa, 56127 Pisa, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudia","family":"Campobasso","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Pisa, 56127 Pisa, Italy"},{"name":"Department of Biosystems, KU Leuven, 3001 Leuven, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9363-0049","authenticated-orcid":false,"given":"Ann-Brit","family":"Klatt","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine\/Infectious Diseases and Pulmonary Medicine, Charit\u00e9-Universit\u00e4tsmedizin Berlin, 13353 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8767-7094","authenticated-orcid":false,"given":"Novella","family":"Cesta","sequence":"additional","affiliation":[{"name":"PhD Course in Microbiology, Immunology, Infectious Diseases and Transplants (MIMIT), University of Rome Tor Vergata, 00133 Rome, Italy"},{"name":"Department of Systems Medicine, University of Rome Tor Vergata, 00133 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Margherita","family":"De Masi","sequence":"additional","affiliation":[{"name":"Department of Systems Medicine, University of Rome Tor Vergata, 00133 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5219-2521","authenticated-orcid":false,"given":"Andrej","family":"Trampuz","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine\/Infectious Diseases and Pulmonary Medicine, Charit\u00e9-Universit\u00e4tsmedizin Berlin, 13353 Berlin, Germany"},{"name":"Center for Musculoskeletal Surgery, Charit\u00e9-Universit\u00e4tsmedizin Berlin, Berlin Institute of Health, Corporate Member of Freie Universit\u00e4t Berlin, Humboldt-Universit\u00e4t zu Berlin, 13353 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5791-686X","authenticated-orcid":false,"given":"Arianna","family":"Tavanti","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Pisa, 56127 Pisa, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0688-034X","authenticated-orcid":false,"given":"Mariagrazia","family":"Di Luca","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Pisa, 56127 Pisa, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,11,22]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"e0022120","DOI":"10.1128\/cmr.00221-20","article-title":"Environmental, Microbiological, and Immunological Features of Bacterial Biofilms Associated with Implanted Medical Devices","volume":"35","author":"Caldara","year":"2022","journal-title":"Clin. 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