{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T15:03:20Z","timestamp":1787497400411,"version":"build-2736575974"},"reference-count":59,"publisher":"MDPI AG","issue":"23","license":[{"start":{"date-parts":[[2022,11,23]],"date-time":"2022-11-23T00:00:00Z","timestamp":1669161600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Doxorubicin (DOX) is an anticancer antibiotic which has various effects in human cancers. It is one of the commonly known causes of drug-induced nephrotoxicity, which results in acute renal injury. Adrenomedullin (ADM), a vasodilator peptide, is widely distributed in many tissues and has potent protective effects. Therefore, the current study aimed to examine the protective potential mechanisms of ADM against DOX-induced nephrotoxicity. A total of 28 male Wistar rats were randomized into four groups: control group, doxorubicin group (15 mg\/kg single intraperitoneal injection of DOX), adrenomedullin + doxorubicin group (12 \u03bcg\/kg\/day intraperitoneal injection of ADM) 3 days prior to DOX injection and continuing for 14 days after the model was established, and adrenomedullin group. Kidney function biomarkers, oxidative stress markers, and inflammatory mediators (TNF-\u03b1, NLRP3, IL-1\u03b2, and IL-18) were assessed. The expressions of gasdermin D and ASC were assessed by real-time PCR. Furthermore, the abundances of caspase-1 (p20), Bcl-2, and Bax immunoreactivity were evaluated. ADM administration improved the biochemical parameters of DOX-induced nephrotoxicity, significantly reduced oxidative damage markers and inflammatory mediators, and suppressed both apoptosis and pyroptosis. These results were confirmed by the histopathological findings and revealed that ADM\u2019s antioxidant, anti-inflammatory, anti-apoptotic, and anti-pyroptotic properties may have prospective applications in the amelioration of DOX-induced nephrotoxicity.<\/jats:p>","DOI":"10.3390\/ijms232314570","type":"journal-article","created":{"date-parts":[[2022,11,23]],"date-time":"2022-11-23T02:10:54Z","timestamp":1669169454000},"page":"14570","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":37,"title":["Adrenomedullin Mitigates Doxorubicin-Induced Nephrotoxicity in Rats: Role of Oxidative Stress, Inflammation, Apoptosis, and Pyroptosis"],"prefix":"10.3390","volume":"23","author":[{"given":"Rania Nagi","family":"Abd-Ellatif","sequence":"first","affiliation":[{"name":"Medical Biochemistry Department, Faculty of Medicine, Tanta University, Tanta 31527, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nahla Anas","family":"Nasef","sequence":"additional","affiliation":[{"name":"Medical Biochemistry Department, Faculty of Medicine, Tanta University, Tanta 31527, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hemat El-Sayed","family":"El-Horany","sequence":"additional","affiliation":[{"name":"Medical Biochemistry Department, Faculty of Medicine, Tanta University, Tanta 31527, Egypt"},{"name":"Biochemistry Department, College of Medicine, Ha\u2019il University, Ha\u2019il 2440, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marwa Nagy","family":"Emam","sequence":"additional","affiliation":[{"name":"Physiology Department, Faculty of Medicine, Tanta University, Tanta 31527, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Reham Lotfy","family":"Younis","sequence":"additional","affiliation":[{"name":"Physiology Department, Faculty of Medicine, Tanta University, Tanta 31527, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2335-3699","authenticated-orcid":false,"given":"Rehab E. Abo","family":"El Gheit","sequence":"additional","affiliation":[{"name":"Physiology Department, Faculty of Medicine, Tanta University, Tanta 31527, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Walaa","family":"Elseady","sequence":"additional","affiliation":[{"name":"Anatomy and Embryology Department, Faculty of Medicine, Tanta University, Tanta 31527, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Doaa A.","family":"Radwan","sequence":"additional","affiliation":[{"name":"Anatomy and Embryology Department, Faculty of Medicine, Tanta University, Tanta 31527, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasser Mostafa","family":"Hafez","sequence":"additional","affiliation":[{"name":"Internal Medicine Department, Faculty of Medicine, Tanta University, Tanta 31527, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmad","family":"Eissa","sequence":"additional","affiliation":[{"name":"Internal Medicine Department, Faculty of Medicine, Tanta University, Tanta 31527, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alshimaa","family":"Aboalsoud","sequence":"additional","affiliation":[{"name":"Pharmacology Department, Faculty of Medicine, Tanta University, Tanta 31527, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rania H.","family":"Shalaby","sequence":"additional","affiliation":[{"name":"Pharmacology Department, Faculty of Medicine, Tanta University, Tanta 31527, Egypt"},{"name":"Dubai Medical College for Girls, Dubai 20170, United Arab Emirates"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marwa Mohamed","family":"Atef","sequence":"additional","affiliation":[{"name":"Medical Biochemistry Department, Faculty of Medicine, Tanta University, Tanta 31527, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,11,23]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Arunachalam, S., Meeran, M.F.N., Azimullah, S., Jha, N.K., Saraswathiamma, D., Subramanya, S., Albawardi, A., and Ojha, S. (2022). \u03b1-Bisabolol Attenuates Doxorubicin Induced Renal Toxicity by Modulating NF-\u03baB\/MAPK Signaling and Caspase-Dependent Apoptosis in Rats. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms231810528"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"110111","DOI":"10.1016\/j.cbi.2022.110111","article-title":"Investigation of the multi-targeted protection potential of tannic acid against doxorubicin-induced kidney damage in rats","volume":"365","author":"Yesilkent","year":"2022","journal-title":"Chem.-Biol. Interact."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Mohamed, H.K., Mobasher, M.A., Ebiya, R.A., Hassen, M.T., Hagag, H.M., El-Sayed, R., Abdel-Ghany, S., Said, M.M., and Awad, N.S. (2022). Anti-Inflammatory, Anti-Apoptotic, and Antioxidant Roles of Honey, Royal Jelly, and Propolis in Suppressing Nephrotoxicity Induced by Doxorubicin in Male Albino Rats. Antioxidants, 11.","DOI":"10.3390\/antiox11051029"},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Harber, M. (2022). Acute Kidney Injury Epidemiology and Causes. Primer on Nephrology, Springer.","DOI":"10.1007\/978-3-030-76419-7"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1159\/000486625","article-title":"Prevention of doxorubicin-induced renal toxicity by theanine in rats","volume":"101","author":"Nagai","year":"2018","journal-title":"Pharmacology"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"548","DOI":"10.1080\/01480545.2020.1730885","article-title":"Montelukast ameliorates doxorubicin-induced cardiotoxicity via modulation of p-glycoprotein and inhibition of ROS-mediated TNF-\u03b1\/NF-\u03baB pathways","volume":"45","author":"Hafez","year":"2022","journal-title":"Drug Chem. Toxicol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"364","DOI":"10.1038\/s41420-022-01144-9","article-title":"Long noncoding RNA NONMMUT015745 inhibits doxorubicin-mediated cardiomyocyte apoptosis by regulating Rab2A-p53 axis","volume":"8","author":"Cai","year":"2022","journal-title":"Cell Death Discov."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"2757","DOI":"10.1111\/jcmm.17277","article-title":"Multi-faceted role of pyroptosis mediated by inflammasome in liver fibrosis","volume":"26","author":"Yang","year":"2022","journal-title":"J. Cell. Mol. Med."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Zhao, H., Liu, H., Yang, Y., and Wang, H. (2022). The Role of Autophagy and Pyroptosis in Liver Disorders. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms23116208"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"167290","DOI":"10.1016\/j.jmb.2021.167290","article-title":"Pyroptosis in kidney disease","volume":"434","author":"Wang","year":"2022","journal-title":"J. Mol. Biol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"167461","DOI":"10.1016\/j.jmb.2022.167461","article-title":"Molecular mechanisms and functions of pyroptosis","volume":"434","author":"Shao","year":"2022","journal-title":"J. Mol. Biol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"6321","DOI":"10.1038\/s41467-022-34036-8","article-title":"Enhancing Gasdermin-induced tumor pyroptosis through preventing ESCRT-dependent cell membrane repair augments antitumor immune response","volume":"13","author":"Li","year":"2022","journal-title":"Nat. Commun."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"679","DOI":"10.1038\/s41586-019-1752-8","article-title":"Activity of caspase-8 determines plasticity between cell death pathways","volume":"575","author":"Newton","year":"2019","journal-title":"Nature"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"3068","DOI":"10.1016\/j.jmb.2018.07.002","article-title":"Mechanisms of gasdermin family members in inflammasome signaling and cell death","volume":"430","author":"Feng","year":"2018","journal-title":"J. Mol. Biol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1038\/s41419-021-03431-2","article-title":"Gasdermin E deficiency attenuates acute kidney injury by inhibiting pyroptosis and inflammation","volume":"12","author":"Xia","year":"2021","journal-title":"Cell Death Dis."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1038\/s41421-020-0167-x","article-title":"Inflammasome activation and regulation: Toward a better understanding of complex mechanisms","volume":"6","author":"Zheng","year":"2020","journal-title":"Cell Discov."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"79","DOI":"10.15698\/cst2022.10.272","article-title":"Pyroptosis in NLRP3 inflammasome-related atherosclerosis","volume":"6","author":"Zeng","year":"2022","journal-title":"Cell Stress"},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Sol\u00e9-Ribalta, A., Bobillo-P\u00e9rez, S., and Jordan-Garc\u00eda, I. (2022). A Review of Adrenomedullin in Pediatric Patients: A Useful Biomarker. Children, 9.","DOI":"10.3390\/children9081181"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1016\/j.jss.2010.03.064","article-title":"Attenuation of renal ischemia and reperfusion injury by human adrenomedullin and its binding protein","volume":"163","author":"Shah","year":"2010","journal-title":"J. Surg. Res."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1630","DOI":"10.1016\/j.juro.2009.12.002","article-title":"Adrenomedullin increases renal nitric oxide production and ameliorates renal injury in mice with unilateral ureteral obstruction","volume":"183","author":"Ito","year":"2010","journal-title":"J. Urol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"489","DOI":"10.1038\/s41419-019-1728-5","article-title":"Adrenomedullin alleviates the pyroptosis of Leydig cells by promoting autophagy via the ROS\u2013AMPK\u2013mTOR axis","volume":"10","author":"Li","year":"2019","journal-title":"Cell Death Dis."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"624","DOI":"10.1002\/tox.22075","article-title":"Dual effects of quercetin in doxorubicin-induced nephrotoxicity in rats and its modulation of the cytotoxic activity of doxorubicin on human carcinoma cells","volume":"31","author":"Heeba","year":"2016","journal-title":"Environ. Toxicol."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Hussain, M.A., Abogresha, N.M., AbdelKader, G., Hassan, R., Abdelaziz, E.Z., and Greish, S.M. (2021). Antioxidant and anti-inflammatory effects of crocin ameliorate doxorubicin-induced nephrotoxicity in rats. Oxidative Med. Cell. Longev., 2021.","DOI":"10.1155\/2021\/8841726"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"823","DOI":"10.1111\/jdi.13218","article-title":"Adrenomedullin has a cytoprotective role against endoplasmic reticulum stress for pancreatic \u03b2-cells in autocrine and paracrine manners","volume":"11","author":"Suetomi","year":"2020","journal-title":"J. Diabetes Investig."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"347","DOI":"10.1016\/j.carbpol.2018.06.109","article-title":"Preparation and renoprotective effects of carboxymethyl chitosan oligosaccharide on adriamycin nephropathy","volume":"201","author":"Qiao","year":"2018","journal-title":"Carbohydr. Polym."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"108777","DOI":"10.1016\/j.cbi.2019.108777","article-title":"Nicorandil combats doxorubicin\u2013induced nephrotoxicity via amendment of TLR4\/P38 MAPK\/NF\u03ba-B signaling pathway","volume":"311","author":"Khames","year":"2019","journal-title":"Chem.-Biol. Interact."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"F558","DOI":"10.1152\/ajprenal.00137.2018","article-title":"Adiponectin attenuates kidney injury and fibrosis in deoxycorticosterone acetate-salt and angiotensin II-induced CKD mice","volume":"315","author":"Tian","year":"2018","journal-title":"Am. J. Physiol.-Ren. Physiol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"370","DOI":"10.17582\/journal.aavs\/2020\/8.4.370.384","article-title":"Effects of rutin and quercetin on doxorubicin-induced renocardiotoxicity in male wistar rats","volume":"8","author":"Mahmoud","year":"2020","journal-title":"Adv. Anim. Vet. Sci."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"005","DOI":"10.1055\/s-0031-1293607","article-title":"The protective effect of adrenomedullin on renal injury, in a model of abdominal aorta cross-clamping","volume":"60","author":"Oyar","year":"2012","journal-title":"Thorac. Cardiovasc. Surg."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"111224","DOI":"10.1016\/j.mce.2021.111224","article-title":"Experimental study on renoprotective effect of intermedin on diabetic nephropathy","volume":"528","author":"Wang","year":"2021","journal-title":"Mol. Cell. Endocrinol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"448","DOI":"10.1080\/13813455.2017.1422766","article-title":"Chemoprotective effects of curcumin on doxorubicin-induced nephrotoxicity in wistar rats: By modulating inflammatory cytokines, apoptosis, oxidative stress and oxidative DNA damage","volume":"124","author":"Benzer","year":"2018","journal-title":"Arch. Physiol. Biochem."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"739","DOI":"10.1007\/s00210-019-01782-4","article-title":"Protection from doxorubicin-induced nephrotoxicity by clindamycin: Novel antioxidant, anti-inflammatory and anti-apoptotic roles","volume":"393","author":"Ibrahim","year":"2020","journal-title":"Naunyn-Schmiedeberg\u2019s Arch. Pharmacol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1358\/dnp.2005.18.3.892764","article-title":"Adrenomedullin as a potent antioxidative and antiatherosclerotic substance","volume":"18","author":"Shimosawa","year":"2005","journal-title":"Drug News Perspect."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"2251","DOI":"10.1016\/j.sjbs.2020.07.011","article-title":"Doxorubicin-induced alterations in kidney functioning, oxidative stress, DNA damage, and renal tissue morphology; Improvement by Acacia hydaspica tannin-rich ethyl acetate fraction","volume":"27","author":"Afsar","year":"2020","journal-title":"Saudi J. Biol. Sci."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/j.peptides.2014.08.013","article-title":"Pathophysiological roles of adrenomedullin-RAMP2 system in acute and chronic cerebral ischemia","volume":"62","author":"Igarashi","year":"2014","journal-title":"Peptides"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"e22725","DOI":"10.1002\/jbt.22725","article-title":"Potential effect of adrenomedullin on metabolic and endocrinal dysfunctions in the experimentally induced polycystic ovary: Targeting implication of endoplasmic reticulum stress","volume":"35","author":"Barhoma","year":"2021","journal-title":"J. Biochem. Mol. Toxicol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"111308","DOI":"10.1016\/j.biopha.2021.111308","article-title":"Apigenin ameliorates doxorubicin-induced renal injury via inhibition of oxidative stress and inflammation","volume":"137","author":"Wu","year":"2021","journal-title":"Biomed. Pharmacother."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/j.yjmcc.2019.08.009","article-title":"Doxorubicin induces cardiomyocyte pyroptosis via the TINCR-mediated posttranscriptional stabilization of NLR family pyrin domain containing 3","volume":"136","author":"Meng","year":"2019","journal-title":"J. Mol. Cell. Cardiol."},{"key":"ref_39","first-page":"1197","article-title":"Adrenomedullin protects rat dorsal root ganglion neurons against doxorubicin-induced toxicity by ameliorating oxidative stress","volume":"23","author":"Mahmoodazdeh","year":"2020","journal-title":"Iran. J. Basic Med. Sci."},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"An, R., Xi, C., Xu, J., Liu, Y., Zhang, S., Wang, Y., Hao, Y., and Sun, L. (2017). Intramyocardial injection of recombinant adeno-associated viral vector coexpressing PR39\/adrenomedullin enhances angiogenesis and reduces apoptosis in a rat myocardial infarction model. Oxidative Med. Cell. Longev., 2017.","DOI":"10.1155\/2017\/1271670"},{"key":"ref_41","first-page":"3342","article-title":"Overexpression of adrenomedullin protects mesenchymal stem cells against hypoxia and serum deprivation-induced apoptosis via the Akt\/GSK3\u03b2 and Bcl-2 signaling pathways","volume":"41","author":"Si","year":"2018","journal-title":"Int. J. Mol. Med."},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Zheng, Z., and Li, G. (2020). Mechanisms and therapeutic regulation of pyroptosis in inflammatory diseases and cancer. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21041456"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1016\/j.jsbmb.2018.05.003","article-title":"Impact of steroid hormones E2 and P on the NLRP3\/ASC\/Casp1 axis in primary mouse astroglia and BV-2 cells after in vitro hypoxia","volume":"183","author":"Slowik","year":"2018","journal-title":"J. Steroid Biochem. Mol. Biol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"660","DOI":"10.1038\/nature15514","article-title":"Cleavage of GSDMD by inflammatory caspases determines pyroptotic cell death","volume":"526","author":"Shi","year":"2015","journal-title":"Nature"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1038\/s41419-021-03614-x","article-title":"Regulated cell death pathways in doxorubicin-induced cardiotoxicity","volume":"12","author":"Christidi","year":"2021","journal-title":"Cell Death Dis."},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Diao, C., Chen, Z., Qiu, T., Liu, H., Yang, Y., Liu, X., Wu, J., and Wang, L. (2019). Inhibition of PRMT5 attenuates oxidative stress-induced pyroptosis via activation of the Nrf2\/HO-1 signal pathway in a mouse model of renal ischemia-reperfusion injury. Oxidative Med. Cell. Longev., 2019.","DOI":"10.1155\/2019\/2345658"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"1689","DOI":"10.1038\/s41467-019-09397-2","article-title":"Gasdermin pores permeabilize mitochondria to augment caspase-3 activation during apoptosis and inflammasome activation","volume":"10","author":"Rogers","year":"2019","journal-title":"Nat. Commun."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"622","DOI":"10.1080\/10520295.2022.2111465","article-title":"Thymoquinone alleviates doxorubicin induced acute kidney injury by decreasing endoplasmic reticulum stress, inflammation and apoptosis","volume":"97","author":"Kaymak","year":"2022","journal-title":"Biotech. Histochem."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"27","DOI":"10.4103\/0976-0105.177703","article-title":"A simple practice guide for dose conversion between animals and humans","volume":"7","author":"Nair","year":"2016","journal-title":"J. Basic Clin. Pharm."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"829","DOI":"10.1139\/cjpp-2015-0364","article-title":"Renoprotective effects of aliskiren on adenine-induced tubulointerstitial nephropathy: Possible underlying mechanisms","volume":"94","author":"Hussein","year":"2016","journal-title":"Can. J. Physiol. Pharmacol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1016\/0003-2697(79)90738-3","article-title":"Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction","volume":"95","author":"Ohkawa","year":"1979","journal-title":"Anal. Biochem."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"389","DOI":"10.1016\/0003-2697(72)90132-7","article-title":"Colorimetric assay of catalase","volume":"47","author":"Sinha","year":"1972","journal-title":"Anal. Biochem."},{"key":"ref_53","unstructured":"Larson, A., Orrenius, S., Holmgren, A., and Mannerwik, B. (1983). Active transport of glutathione disulfide from erythrocytes. Functions of Glutathione, Biochemical, Physiological, Toxicological and Clinical Aspects, Raven Press."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1007\/s10616-011-9395-0","article-title":"An improved non-enzymatic \u201cDNA ladder assay\u201d for more sensitive and early detection of apoptosis","volume":"64","author":"Suman","year":"2012","journal-title":"Cytotechnology"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real-time quantitative PCR and the 2\u2212\u0394\u0394CT method","volume":"25","author":"Livak","year":"2001","journal-title":"Methods"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"115340","DOI":"10.1016\/j.taap.2020.115340","article-title":"Octreotide and melatonin alleviate inflammasome-induced pyroptosis through inhibition of TLR4-NF-\u03baB-NLRP3 pathway in hepatic ischemia\/reperfusion injury","volume":"410","author":"El","year":"2021","journal-title":"Toxicol. Appl. Pharmacol."},{"key":"ref_57","doi-asserted-by":"crossref","unstructured":"Buchwalow, I.B., and B\u00f6cker, W. (2010). Immunohistochemistry: Basics and Methods, Springer.","DOI":"10.1007\/978-3-642-04609-4"},{"key":"ref_58","unstructured":"Suvarna, K.S., Layton, C., and Bancroft, J.D. (2018). Bancroft\u2019s theory and practice of histological techniques E-Book. Elsevier Health Sciences."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"1524","DOI":"10.1016\/j.biopha.2018.09.171","article-title":"Protective effects of L-theanine against doxorubicin-induced nephrotoxicity in rats","volume":"108","author":"Kural","year":"2018","journal-title":"Biomed. Pharmacother."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/23\/23\/14570\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T01:24:46Z","timestamp":1760145886000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/23\/23\/14570"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,11,23]]},"references-count":59,"journal-issue":{"issue":"23","published-online":{"date-parts":[[2022,12]]}},"alternative-id":["ijms232314570"],"URL":"https:\/\/doi.org\/10.3390\/ijms232314570","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,11,23]]}}}