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In this work, we used previously developed genetically modified strains of Trypanosoma\u00a0cruzi (T. cruzi), hemi-knockout (KO +\/\u2212) and overexpressing (OE) the TcNTPDase-1 gene to evaluate the parasite infectivity profile in a mouse model of acute infection (n = 6 mice per group). Our results showed significantly higher parasitemia and mortality, and lower weight in animals infected with parasites OE TcNTPDase-1, as compared to the infection with the wild type (WT) parasites. On the other hand, animals infected with (KO +\/\u2212) parasites showed no mortality during the 30-day trial and mouse weight was more similar to the non-infected (NI) animals. In addition, they had low parasitemia (45.7 times lower) when compared with parasites overexpressing TcNTPDase-1 from the hemi-knockout (OE KO +\/\u2212) group. The hearts of animals infected with the OE KO +\/\u2212 and OE parasites showed significantly larger regions of cardiac inflammation than those infected with the WT parasites (p &lt; 0.001). Only animals infected with KO +\/\u2212 did not show individual electrocardiographic changes during the period of experimentation. Together, our results expand the knowledge on the role of NTPDases in T.\u00a0cruzi infectivity, reenforcing the potential of this enzyme as a chemotherapy target to treat Chagas disease (CD).<\/jats:p>","DOI":"10.3390\/ijms232314661","type":"journal-article","created":{"date-parts":[[2022,11,24]],"date-time":"2022-11-24T03:58:16Z","timestamp":1669262296000},"page":"14661","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Overexpression of TcNTPDase-1 Gene Increases Infectivity in Mice Infected with Trypanosoma cruzi"],"prefix":"10.3390","volume":"23","author":[{"given":"Nat\u00e1lia Lins da","family":"Silva-Gomes","sequence":"first","affiliation":[{"name":"Plataforma de PCR em Tempo Real RPT09A, Laborat\u00f3rio de Virologia Molecular-IOC\/FIOCRUZ, Rio de Janeiro 21040-360, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leonardo Alexandre de Souza","family":"Ruivo","sequence":"additional","affiliation":[{"name":"Plataforma de PCR em Tempo Real RPT09A, Laborat\u00f3rio de Virologia Molecular-IOC\/FIOCRUZ, Rio de Janeiro 21040-360, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claudia","family":"Moreira","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Biologia Molecular de Tripanossomat\u00eddeos-ICC\/FIOCRUZ, Curitiba 81350-010, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2399-9967","authenticated-orcid":false,"given":"Marcelo","family":"Meuser-Batista","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Educa\u00e7\u00e3o Profissional em T\u00e9cnicas Laboratoriais em Sa\u00fade, EPSJV\/FIOCRUZ, Rio de Janeiro 21040-360, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cristiane Fran\u00e7a da","family":"Silva","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Biologia Celular-IOC\/FIOCRUZ, Rio de Janeiro 21040-360, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Denise da Gama Jaen","family":"Batista","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Biologia Celular-IOC\/FIOCRUZ, Rio de Janeiro 21040-360, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"St\u00eanio","family":"Fragoso","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Biologia Molecular de Tripanossomat\u00eddeos-ICC\/FIOCRUZ, Curitiba 81350-010, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gabriel Melo de","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Biologia Celular-IOC\/FIOCRUZ, Rio de Janeiro 21040-360, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria de Nazar\u00e9 Correia","family":"Soeiro","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Biologia Celular-IOC\/FIOCRUZ, Rio de Janeiro 21040-360, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5340-8697","authenticated-orcid":false,"given":"Otacilio C.","family":"Moreira","sequence":"additional","affiliation":[{"name":"Plataforma de PCR em Tempo Real RPT09A, Laborat\u00f3rio de Virologia Molecular-IOC\/FIOCRUZ, Rio de Janeiro 21040-360, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,11,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1016\/j.idc.2012.03.002","article-title":"American Trypanosomiasis (Chagas Disease)","volume":"26","author":"Rassi","year":"2012","journal-title":"Infect. 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