{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,22]],"date-time":"2026-01-22T13:21:54Z","timestamp":1769088114969,"version":"3.49.0"},"reference-count":56,"publisher":"MDPI AG","issue":"23","license":[{"start":{"date-parts":[[2022,11,25]],"date-time":"2022-11-25T00:00:00Z","timestamp":1669334400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Open Competition Mechanism in order to select the best candidates\u2019 foundation for the breeding industry prosperity of Jiangsu Province, China","award":["JBGS [2021]098"],"award-info":[{"award-number":["JBGS [2021]098"]}]},{"name":"Open Competition Mechanism in order to select the best candidates\u2019 foundation for the breeding industry prosperity of Jiangsu Province, China","award":["BE2019341"],"award-info":[{"award-number":["BE2019341"]}]},{"name":"Open Competition Mechanism in order to select the best candidates\u2019 foundation for the breeding industry prosperity of Jiangsu Province, China","award":["2021M702764"],"award-info":[{"award-number":["2021M702764"]}]},{"name":"Key Research and Development Project (Modern Agriculture) of Jiangsu Province","award":["JBGS [2021]098"],"award-info":[{"award-number":["JBGS [2021]098"]}]},{"name":"Key Research and Development Project (Modern Agriculture) of Jiangsu Province","award":["BE2019341"],"award-info":[{"award-number":["BE2019341"]}]},{"name":"Key Research and Development Project (Modern Agriculture) of Jiangsu Province","award":["2021M702764"],"award-info":[{"award-number":["2021M702764"]}]},{"DOI":"10.13039\/501100002858","name":"China Postdoctoral Science Foundation","doi-asserted-by":"publisher","award":["JBGS [2021]098"],"award-info":[{"award-number":["JBGS [2021]098"]}],"id":[{"id":"10.13039\/501100002858","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002858","name":"China Postdoctoral Science Foundation","doi-asserted-by":"publisher","award":["BE2019341"],"award-info":[{"award-number":["BE2019341"]}],"id":[{"id":"10.13039\/501100002858","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002858","name":"China Postdoctoral Science Foundation","doi-asserted-by":"publisher","award":["2021M702764"],"award-info":[{"award-number":["2021M702764"]}],"id":[{"id":"10.13039\/501100002858","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Jiangsu Higher Education Institutions","award":["JBGS [2021]098"],"award-info":[{"award-number":["JBGS [2021]098"]}]},{"name":"Jiangsu Higher Education Institutions","award":["BE2019341"],"award-info":[{"award-number":["BE2019341"]}]},{"name":"Jiangsu Higher Education Institutions","award":["2021M702764"],"award-info":[{"award-number":["2021M702764"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Post-weaning diarrhea caused by enterotoxigenic Escherichia coli F18 (E. coli F18) causes significant economic losses for pig producers. Fucosyltransferase 8 (FUT8) is a glycosyltransferase that catalyzes core fucosylation; however, its role in mediating the resistance to E. coli F18 infection in pigs remains unknown. In this study, we systematically verified the relationship between FUT8 expression and E. coli resistance. The results showed that FUT8 was expressed in all detected tissues of Meishan piglets and that its expression was significantly increased in the duodenum and jejunum of E. coli F18-sensitive individuals when compared to E. coli F18-resistant individuals. FUT8 expression increased after exposure to E. coli F18 (p &lt; 0.05) and decreased significantly after LPS induction for 6 h (p &lt; 0.01). Then, the IPEC-J2 stable cell line with FUT8 interference was constructed, and FUT8 knockdown decreased the adhesion of E. coli F18ac to IPEC-J2 cells (p &lt; 0.05). Moreover, we performed a comparative transcriptome study of IPEC-J2 cells after FUT8 knockdown via RNA-seq. In addition, further expression verification demonstrated the significant effect of FUT8 on the glycosphingolipid biosynthesis and Toll-like signaling pathways. Moreover, the core promoter of FUT8, which was located at \u22121213 bp to \u2212673 bp, was identified via luciferase assay. Interestingly, we found a 1 bp C base insertion mutation at the \u2212774 bp region, which could clearly inhibit the transcriptional binding activity of C\/EBP\u03b1 to an FUT8 promoter. Therefore, it is speculated that FUT8 acts in a critical role in the process of E. coli infection; furthermore, the low expression of FUT8 is conducive to the enhancement of E. coli resistance in piglets. Our findings revealed the mechanism of pig FUT8 in regulating E. coli resistance, which provided a theoretical basis for the screening of E. coli resistance in Chinese local pig breeds.<\/jats:p>","DOI":"10.3390\/ijms232314713","type":"journal-article","created":{"date-parts":[[2022,11,25]],"date-time":"2022-11-25T03:00:13Z","timestamp":1669345213000},"page":"14713","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Effect and Mechanism Analysis of Pig FUT8 Gene on Resistance to Escherichia coli F18 Infection"],"prefix":"10.3390","volume":"23","author":[{"given":"Lisi","family":"Wu","sequence":"first","affiliation":[{"name":"Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design of Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yifu","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design of Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shenglong","family":"Wu","sequence":"additional","affiliation":[{"name":"Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design of Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China"},{"name":"Joint International Research Laboratory of Agriculture & Agri-Product Safety, Yangzhou University, Yangzhou 225009, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhengchang","family":"Wu","sequence":"additional","affiliation":[{"name":"Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design of Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5486-2253","authenticated-orcid":false,"given":"Wenbin","family":"Bao","sequence":"additional","affiliation":[{"name":"Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design of Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China"},{"name":"Joint International Research Laboratory of Agriculture & Agri-Product Safety, Yangzhou University, Yangzhou 225009, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,11,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2077","DOI":"10.1007\/s11538-008-9348-8","article-title":"Persistence and spread of gastro-intestinal infections: The case of enterotoxigenic Escherichia coli in piglets","volume":"70","author":"Boldin","year":"2008","journal-title":"Bull. Math. Biol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1016\/0378-1135(94)90111-2","article-title":"Prevalence of F107 fimbriae on Escherichia coli isolated from pigs with oedema disease or postweaning diarrhoea","volume":"40","author":"Imberechts","year":"1994","journal-title":"Vet. Microbiol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1079\/AHR2005105","article-title":"Escherichia coli in postweaning diarrhea in pigs: An update on bacterial types, pathogenesis, and prevention strategies","volume":"6","author":"Fairbrother","year":"2005","journal-title":"Anim. Health Res. Rev."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1307","DOI":"10.1007\/s11250-019-01934-x","article-title":"Resistance to ETEC F4\/F18-mediated piglet diarrhoea: Opening the gene black box","volume":"51","author":"Sinha","year":"2019","journal-title":"Trop. Anim. Health Prod."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"804","DOI":"10.1016\/j.ccell.2017.05.007","article-title":"A Systems Biology Approach Identifies FUT8 as a Driver of Melanoma Metastasis","volume":"31","author":"Agrawal","year":"2017","journal-title":"Cancer Cell."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"129870","DOI":"10.1016\/j.bbagen.2021.129870","article-title":"1,6-Fucosyltransferase contributes to cell migration and proliferation as well as to cancer stemness features in pancreatic carcinoma","volume":"1865","author":"Liang","year":"2021","journal-title":"Biochim. Biophys. Acta Gen. Subj."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Bastian, K., Scott, E., Elliott, D.J., and Munkley, J. (2021). FUT8 Alpha-(1,6)-Fucosyltransferase in Cancer. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms22010455"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"606","DOI":"10.1016\/j.jmii.2020.02.006","article-title":"Loss of core fucosylation suppressed the humoral immune response in Salmonella typhimurium infected mice","volume":"54","author":"Zahid","year":"2021","journal-title":"J. Microbiol. Immunol. Infect."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"2596","DOI":"10.4049\/jimmunol.1402678","article-title":"Core fucosylation of IgG B cell receptor is required for antigen recognition and antibody production","volume":"194","author":"Li","year":"2015","journal-title":"J. Immunol."},{"key":"ref_10","first-page":"5","article-title":"Intestinal microbial structure of core fucosyltransferase FUT8 gene knockout mice","volume":"29","author":"Li","year":"2017","journal-title":"Chin. J. Microecol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"888","DOI":"10.1111\/j.1349-7006.2009.01125.x","article-title":"Core fucosylation of E-cadherin enhances cell\u2013cell adhesion in human colon carcinoma WiDr cells","volume":"100","author":"Osumi","year":"2009","journal-title":"Cancer Sci."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"3190","DOI":"10.1111\/cas.14987","article-title":"Fucosyltransferase 8 regulation and breast cancer suppression by transcription factor activator protein 2\u03b3","volume":"112","author":"Ma","year":"2021","journal-title":"Cancer Sci."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"129596","DOI":"10.1016\/j.bbagen.2020.129596","article-title":"Involvement of the \u03b1-helical and Src homology 3 domains in the molecular assembly and enzymatic activity of human \u03b11, 6-fucosyltransferase, FUT8","volume":"1864","author":"Ihara","year":"2020","journal-title":"Biochim. Biophys. Acta. Gen. Subj."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1620","DOI":"10.1053\/j.gastro.2016.03.002","article-title":"Core Fucosylation on T Cells, Required for Activation of T-Cell Receptor Signaling and Induction of Colitis in Mice, Is Increased in Patients with Inflammatory Bowel Disease","volume":"150","author":"Fujii","year":"2016","journal-title":"Gastroenterology"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1093\/jb\/mvy098","article-title":"Core fucose is essential glycosylation for CD14-dependent Toll-like receptor 4 and Toll-like receptor 2 signalling in macrophages","volume":"165","author":"Nakayama","year":"2019","journal-title":"J. Biochem."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"24710","DOI":"10.18632\/aging.203711","article-title":"Detection of genomic structure variants associated with wrinkled skin in Xiang pig by next generation sequencing","volume":"13","author":"Xiaoli","year":"2021","journal-title":"Aging"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"24126","DOI":"10.3390\/ijms141224126","article-title":"Transcriptional activity of the FUT1 gene promoter region in pigs","volume":"14","author":"Zi","year":"2013","journal-title":"Int. J. Mol. Sci."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1016\/bs.acc.2016.11.002","article-title":"PCR-Based Detection Methods for Single-Nucleotide Polymorphism or Mutation: Real-Time PCR and Its Substantial Contribution Toward Technological Refinement","volume":"80","author":"Matsuda","year":"2017","journal-title":"Adv. Clin. Chem."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1007\/978-1-60327-411-1_1","article-title":"SNPs: Impact on gene function and phenotype","volume":"578","author":"Shastry","year":"2009","journal-title":"Methods Mol. Biol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"428","DOI":"10.1111\/age.12428","article-title":"Expression of key glycosphingolipid biosynthesis-globo series pathway genes in Escherichia coli F18-resistant and Escherichia coli F18-sensitive piglets","volume":"47","author":"Dong","year":"2016","journal-title":"Anim. Genet."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Wu, Z., Fan, H., Jin, J., Gao, S., Huang, R., Wu, S., and Bao, W. (2022). Insight into mechanisms of pig lncRNA FUT3-AS1 regulating E. coli F18-bacterial diarrhea. PLoS Pathog., 18.","DOI":"10.1371\/journal.ppat.1010584"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1128\/jcm.35.1.20-24.1997","article-title":"Molecular characterization of Escherichia coli strains isolated from pigs with edema disease","volume":"35","author":"Aarestrup","year":"1997","journal-title":"J. Clin. Microbiol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"4516","DOI":"10.1128\/IAI.71.8.4516-4525.2003","article-title":"Characteriza-tion of the novel factor paa involved in the early steps of the adhesion mechanism of attaching and effacing Escherichia coli","volume":"71","author":"Batisson","year":"2003","journal-title":"Infect. Immun."},{"key":"ref_24","first-page":"259","article-title":"Enterotoxigenic Escherichia coli (ETEC) in farm animals","volume":"30","author":"Nagy","year":"1999","journal-title":"Vet. Res."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"3552","DOI":"10.1128\/IAI.05033-11","article-title":"Attaching and effacing bacterial effector NleC suppresses epithelial inflammatory responses by inhibiting NF-\u03baB and p38 mitogen-activated protein kinase activation","volume":"79","author":"Sham","year":"2011","journal-title":"Infect. Immun."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"18434","DOI":"10.1074\/jbc.M110.172536","article-title":"a1,6-Fucosyltransferase-deficient mice exhibit multiple behavioral abnormalities associated with a schizophrenia-like phenotype: Importance of the balance between the dopamine and serotonin systems","volume":"286","author":"Fukuda","year":"2011","journal-title":"J. Biol. Chem."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Keeley, T.S., Yang, S., and Lau, E. (2019). The Diverse Contributions of Fucose Linkages in Cancer. Cancers, 11.","DOI":"10.3390\/cancers11091241"},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Li, S., Liu, X.Y., Pan, Q., Wu, J., Liu, Z.H., Wang, Y., Liu, M., and Zhang, X.L. (2019). Hepatitis C Virus-Induced FUT8 Causes 5-FU Drug Resistance in Human Hepatoma Huh7.5.1 Cells. Viruses, 11.","DOI":"10.3390\/v11040378"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1186\/s13058-017-0904-8","article-title":"FUT8 promotes breast cancer cell invasiveness by remodeling TGF-\u03b2 receptor core fucosylation","volume":"19","author":"Tu","year":"2017","journal-title":"Breast Cancer Rev."},{"key":"ref_30","first-page":"816","article-title":"a1,6-Fucosyltransferase (FUT8) regulates the cancer-promoting capacity of cancer-associated fibroblasts (CAFs) by modifying EGFR core fucosylation (CF) in non-small cell lung cancer (NSCLC)","volume":"10","author":"Li","year":"2020","journal-title":"Am. J. Cancer Res."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1007\/s10875-016-0253-x","article-title":"The Elements Steering Pathogenesis in IgG-Mediated Alloimmune Diseases","volume":"36","author":"Sonneveld","year":"2016","journal-title":"J. Clin. Immunol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"832","DOI":"10.4049\/jimmunol.1200854","article-title":"Twist1 regulates Ifng expression in Th1 cells by interfering with Runx3 function","volume":"189","author":"Pham","year":"2012","journal-title":"J. Immunol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"409","DOI":"10.1093\/jb\/mvx010","article-title":"A novel pathogenesis of inflammatory bowel disease from the perspective of glyco-immunology","volume":"161","author":"Shinzaki","year":"2017","journal-title":"J. Biochem."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"2394","DOI":"10.1097\/MIB.0000000000000204","article-title":"Intestinal inflammation and mucosal barrier function","volume":"20","author":"Isabel","year":"2014","journal-title":"Inflamm. Bowel Dis."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1111\/j.1365-2958.2012.08174.x","article-title":"Structural insight in histo-blood group binding by the F18 fimbrial adhesin FedF","volume":"86","author":"Moonens","year":"2012","journal-title":"Mol. Microbiol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"588","DOI":"10.1038\/nrgastro.2014.78","article-title":"The role of glycosylation in IBD","volume":"11","author":"Theodoratou","year":"2014","journal-title":"Nat. Rev. Gastroenterol. Hepatol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1889","DOI":"10.1200\/JCO.2014.56.2736","article-title":"Pooled Analysis of Long-Term Survival Data from Phase II and Phase III Trials of Ipilimumab in Unresectable or Metastatic Melanoma","volume":"33","author":"Schadendorf","year":"2015","journal-title":"J. Clin. Oncol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"2706","DOI":"10.1002\/eji.200324228","article-title":"Regulation of PD-1, PD-L1, and PD-L2 expression during normal and autoimmune responses","volume":"33","author":"Liang","year":"2010","journal-title":"Eur. J. Immunol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1111\/pin.12159","article-title":"Mice lacking \u03b11,3-fucosyltransferase 9 exhibit modulation of in vivo immune responses against pathogens","volume":"64","author":"Kashiwazaki","year":"2014","journal-title":"Pathol. Int."},{"key":"ref_40","first-page":"15","article-title":"Effect of FUT3 gene silencing with miRNA on proliferation, invasion and migration abilities of human KATO-III gastric cancer cell line","volume":"62","author":"Cai","year":"2016","journal-title":"Cell Mol. Biol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"32260","DOI":"10.1074\/jbc.271.50.32260","article-title":"Influence of Lewis \u03b11-3\/4-L-Fucosyltransferase (FUT3) Gene Mutations on Enzyme Activity, Erythrocyte Phenotyping, and Circulating Tumor Marker Sialyl-Lewis a Levels","volume":"271","author":"Orntoft","year":"1996","journal-title":"J. Biol. Chem."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"981","DOI":"10.1586\/14737140.2015.1074862","article-title":"Nivolumab and pembrolizumab as immune-modulating monoclonal antibodies targeting the PD-1 receptor to treat melanoma","volume":"15","author":"Faghfuri","year":"2015","journal-title":"Expert. Rev. Anticancer Ther."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"324","DOI":"10.1038\/nrurol.2016.65","article-title":"The role of glycans in the development and progression of prostate cancer","volume":"13","author":"Munkley","year":"2016","journal-title":"Nat. Rev. Urol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s12016-015-8473-z","article-title":"Toll-Like Receptor Pathways in Autoimmune Diseases","volume":"50","author":"Chen","year":"2016","journal-title":"Clin. Rev. Allergy. Immunol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"1087","DOI":"10.1038\/ni1255","article-title":"Essential function for the kinase TAK1 in innate and adaptive immune responses","volume":"6","author":"Sato","year":"2005","journal-title":"Nat. Immunol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"527","DOI":"10.1111\/cmi.12109","article-title":"Bacterial inhibition of inflammatory responses via TLR-independent mechanisms","volume":"15","author":"Kravchenko","year":"2013","journal-title":"Cell. Microbiol."},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Dai, C., Yang, L., Jin, J., Wang, H., Wu, S.L., and Bao, W.B. (2019). Regulation and Molecular Mechanism of TLR5 on Resistance to Escherichia coli F18 in Weaned Piglets. Animals, 9.","DOI":"10.3390\/ani9100735"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1146\/annurev.immunol.23.021704.115633","article-title":"IPC: Professional type 1 interferon-producing cells and plasmacytoid dendritic cell precursors","volume":"23","author":"Liu","year":"2005","journal-title":"Annu. Rev. Immunol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"288","DOI":"10.1126\/science.1987644","article-title":"CCAAT-enhancer binding protein: A component of a differentiation switch","volume":"251","author":"Umek","year":"1991","journal-title":"Science"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1038\/nri2024","article-title":"Transcription factors in myeloid development: Balancing differentiation with transformation","volume":"7","author":"Rosenbauer","year":"2007","journal-title":"Nat. Rev. Immunol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"853","DOI":"10.1016\/j.immuni.2004.11.006","article-title":"Enhancement of hematopoietic stem cell repopulating capacity and self-renewal in the absence of the transcription factor C\/EBP alpha","volume":"21","author":"Zhang","year":"2004","journal-title":"Immunity"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"5961","DOI":"10.1158\/1078-0432.CCR-21-0986","article-title":"Upregulation of C\/EBP\u03b1 Inhibits Suppressive Activity of Myeloid Cells and Potentiates Antitumor Response in Mice and Patients with Cancer","volume":"27","author":"Hashimoto","year":"2021","journal-title":"Clin. Cancer. Res."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"2548","DOI":"10.1038\/sj.onc.1206360","article-title":"Cell cycle inhibition mediated by the outer surface of the C\/EBPalpha basic region is required but not sufficient for granulopoiesis","volume":"22","author":"Wang","year":"2003","journal-title":"Oncogene"},{"key":"ref_54","first-page":"1608","article-title":"Attack Experiment and Phenotype Analysis of Meishan piglets by E. coli F18 Strain","volume":"45","author":"Wu","year":"2014","journal-title":"Chin. J. Anim. Vet. Sci."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1016\/j.gene.2013.02.051","article-title":"Genetic variation in exon 10 of the BPI gene is associated with Escherichia coli F18 susceptibility in Sutai piglets","volume":"523","author":"Liu","year":"2013","journal-title":"Gene"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"619","DOI":"10.1515\/pjvs-2016-0077","article-title":"Use of fluorescence quantitative polymerase chain reaction (PCR) for the detection of Escherichia coli adhesion to pig intestinal epithelial cells","volume":"19","author":"Dai","year":"2016","journal-title":"Pol. J. Vet. Sci."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/23\/23\/14713\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T01:26:29Z","timestamp":1760145989000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/23\/23\/14713"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,11,25]]},"references-count":56,"journal-issue":{"issue":"23","published-online":{"date-parts":[[2022,12]]}},"alternative-id":["ijms232314713"],"URL":"https:\/\/doi.org\/10.3390\/ijms232314713","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,11,25]]}}}