{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T13:11:14Z","timestamp":1781788274053,"version":"3.54.5"},"reference-count":42,"publisher":"MDPI AG","issue":"23","license":[{"start":{"date-parts":[[2022,11,27]],"date-time":"2022-11-27T00:00:00Z","timestamp":1669507200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Key Research and Development Program of China","award":["2018YFD0901102"],"award-info":[{"award-number":["2018YFD0901102"]}]},{"name":"National Key Research and Development Program of China","award":["2020R10130015"],"award-info":[{"award-number":["2020R10130015"]}]},{"name":"National Key Research and Development Program of China","award":["2022N3004"],"award-info":[{"award-number":["2022N3004"]}]},{"name":"Basic Research Projects of Provincial Public Welfare Research Institutes of Fujian Province","award":["2018YFD0901102"],"award-info":[{"award-number":["2018YFD0901102"]}]},{"name":"Basic Research Projects of Provincial Public Welfare Research Institutes of Fujian Province","award":["2020R10130015"],"award-info":[{"award-number":["2020R10130015"]}]},{"name":"Basic Research Projects of Provincial Public Welfare Research Institutes of Fujian Province","award":["2022N3004"],"award-info":[{"award-number":["2022N3004"]}]},{"name":"Regional development projects of Fujian Province","award":["2018YFD0901102"],"award-info":[{"award-number":["2018YFD0901102"]}]},{"name":"Regional development projects of Fujian Province","award":["2020R10130015"],"award-info":[{"award-number":["2020R10130015"]}]},{"name":"Regional development projects of Fujian Province","award":["2022N3004"],"award-info":[{"award-number":["2022N3004"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Candidate peptides with novel angiotensin-I-converting enzyme (ACE) inhibitor activity were obtained from hydrolysates of Gracilariopsis lemaneiformis by virtual screening method. Our results showed that G. lemaneiformis peptides (GLP) could significantly lower blood pressure in spontaneously hypertensive rats (SHR). At least 101 peptide sequences of GLP were identified by LC-MS\/MS analysis and subjected to virtual screening. A total of 20 peptides with the highest docking score were selected and chemically synthesized in order to verify their ACE-inhibitory activities. Among them, SFYYGK, RLVPVPY, and YIGNNPAKG showed good effects with IC50 values of 6.45 \u00b1 0.22, 9.18 \u00b1 0.42, and 11.23 \u00b1 0.23 \u00b5moL\/L, respectively. Molecular docking studies revealed that three peptides interacted with the active center of ACE by hydrogen bonding, hydrophobic interactions, and electrostatic forces. These peptides could form stable complexes with ACE. Furthermore, SFYYGK, RLVPVPY, and YIGNNPAKG significantly reduced systolic blood pressure (SBP) in SHR. YIGNNPAKG exhibited the highest antihypertensive effect, with the largest decrease in SBP (approximately 23 mmHg). In conclusion, SFYYGK, RLVPVPY, and YIGNNPAKG can function as potent therapeutic candidates for hypertension treatment.<\/jats:p>","DOI":"10.3390\/ijms232314850","type":"journal-article","created":{"date-parts":[[2022,11,30]],"date-time":"2022-11-30T01:52:20Z","timestamp":1669773140000},"page":"14850","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Screening and Molecular Mechanisms of Novel ACE-Inhibitory Peptides from Gracilariopsis lemaneiformis"],"prefix":"10.3390","volume":"23","author":[{"given":"Yongchang","family":"Su","sequence":"first","affiliation":[{"name":"College of Chemical Engineering, Huaqiao University, Xiamen 361021, China"},{"name":"Key Laboratory of Cultivation and High-Value Utilization of Marine Organisms in Fujian Province, National Research and Development Center for Marine Fish Processing (Xiamen), Fisheries Research Institute of Fujian, Xiamen 361013, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6094-5514","authenticated-orcid":false,"given":"Shicheng","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Clinical and Diagnostic Sciences, School of Health Sciences, Oakland University, Rochester, MI 48309, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiashen","family":"Shen","sequence":"additional","affiliation":[{"name":"College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9460-5785","authenticated-orcid":false,"given":"Zhiwei","family":"Yi","sequence":"additional","affiliation":[{"name":"Technology Innovation Center for Exploitation of Marine Biological Resources, Third Institute of Oceanography, Ministry of Natural Resources, Xiamen 361005, 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High-Value Utilization of Marine Organisms in Fujian Province, National Research and Development Center for Marine Fish Processing (Xiamen), Fisheries Research Institute of Fujian, Xiamen 361013, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2372-2837","authenticated-orcid":false,"given":"Kun","family":"Qiao","sequence":"additional","affiliation":[{"name":"Key Laboratory of Cultivation and High-Value Utilization of Marine Organisms in Fujian Province, National Research and Development Center for Marine Fish Processing (Xiamen), Fisheries Research Institute of Fujian, Xiamen 361013, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoting","family":"Chen","sequence":"additional","affiliation":[{"name":"Key Laboratory of Cultivation and High-Value Utilization of Marine Organisms in Fujian Province, National Research and Development Center for Marine Fish Processing (Xiamen), Fisheries Research Institute of 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