{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T04:48:11Z","timestamp":1787028491441,"version":"build-2736575974"},"reference-count":25,"publisher":"MDPI AG","issue":"23","license":[{"start":{"date-parts":[[2022,11,28]],"date-time":"2022-11-28T00:00:00Z","timestamp":1669593600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Natural Science Foundation of China","award":["81841001"],"award-info":[{"award-number":["81841001"]}]},{"name":"National Natural Science Foundation of China","award":["81102752"],"award-info":[{"award-number":["81102752"]}]},{"name":"National Natural Science Foundation of China","award":["2017ZX09101002-001-002"],"award-info":[{"award-number":["2017ZX09101002-001-002"]}]},{"name":"Major National Science and Technology Program of China for Innovative Drugs","award":["81841001"],"award-info":[{"award-number":["81841001"]}]},{"name":"Major National Science and Technology Program of China for Innovative Drugs","award":["81102752"],"award-info":[{"award-number":["81102752"]}]},{"name":"Major National Science and Technology Program of China for Innovative Drugs","award":["2017ZX09101002-001-002"],"award-info":[{"award-number":["2017ZX09101002-001-002"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>In 1972, Nobel laureate Youyou Tu\u2019s research team conducted clinical trials on the dried material of Artemisia annua L. from Beijing extracted by ether and then treated with alkali (called \u201cether neutral dry\u201d), which showed that artemisinin was not the only antimalarial component contained. The biosynthesis of sesquiterpenoids in A. annua has increased exponentially after unremitting cultivation efforts, and the plant resources are now quite different from those in the 1970s. In consideration of emerging artemisinin resistance, it is of great theoretical and practical value to further study the antimalarial activity of A. annua and explore its causes. The purpose of this study is to clarify scientific questions, such as \u201cWhat ingredients are synergistic with artemisinin in A. annua?\u201d, and \u201cAre there non-artemisinin antimalarial ingredients in A. annua?\u201d. In this paper, Beijing wild A. annua was used as a control and two representative cultivation species of A. annua were selected to evaluate the antimalarial activity of the herbal medicine. The antimalarial activity of different extracts on mice was studied using the Peters\u2019 four-day suppressive test. UPLC-Q-TOF-MS was used to obtain mass spectrum data for all samples, and a UNIFI platform was used for identification. A multivariate statistical method was used to screen the different compounds with positive correlations. The antimalarial activity of different components from the ether extract and alkali treatments was determined and antimalarial components other than artemisinin were obtained. A total of 24 flavonoids, 68 sesquiterpenoids and 21 other compounds were identified. Compounds associated with differential antimalarial activity were identified. The material basis for the antimalarial activity of A. annua was clarified. The antimalarial components of A. annua include two categories: first, artemisinin and non-artemisinin antimalarial active components, of which the non-artemisinin antimalarial active components may include 5\u03b1-hydroperoxy-eudesma-4(15),11-diene; second, several antimalarial synergistic ingredients in A. annua, including arteanniun B, arteanniun B analogues and polymethoxy flavonoids.<\/jats:p>","DOI":"10.3390\/ijms232314903","type":"journal-article","created":{"date-parts":[[2022,11,28]],"date-time":"2022-11-28T08:43:03Z","timestamp":1669624983000},"page":"14903","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["A Discovery-Based Metabolomic Approach Using UPLC-Q-TOF-MS\/MS Reveals Potential Antimalarial Compounds Present in Artemisia annua L."],"prefix":"10.3390","volume":"23","author":[{"given":"Henan","family":"Shi","sequence":"first","affiliation":[{"name":"Beijing Key Laboratory of TCM Collateral Disease Theory Research, School of Traditional Chinese Medicine, Capital Medical University, Beijing 100069, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhuqing","family":"Wang","sequence":"additional","affiliation":[{"name":"Beijing Key Laboratory of TCM Collateral Disease Theory Research, School of Traditional Chinese Medicine, Capital Medical University, Beijing 100069, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fujie","family":"Xu","sequence":"additional","affiliation":[{"name":"Beijing Key Laboratory of TCM Collateral Disease Theory Research, School of Traditional Chinese Medicine, Capital Medical University, Beijing 100069, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jialin","family":"Li","sequence":"additional","affiliation":[{"name":"Beijing Key Laboratory of TCM Collateral Disease Theory Research, School of Traditional Chinese Medicine, Capital Medical University, Beijing 100069, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Li","sequence":"additional","affiliation":[{"name":"Beijing Key Laboratory of TCM Collateral Disease Theory Research, School of Traditional Chinese Medicine, Capital Medical University, Beijing 100069, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manyuan","family":"Wang","sequence":"additional","affiliation":[{"name":"Beijing Key Laboratory of TCM Collateral Disease Theory Research, School of Traditional Chinese Medicine, Capital Medical University, Beijing 100069, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,11,28]]},"reference":[{"key":"ref_1","unstructured":"World Health Organization (2022, April 30). 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