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Conventional trypsin digestion was used for separating retinal vasculatures in mouse, rat, and other animal models; however, the trypsin method alone is technically difficult to perform and has not been reported in zebrafish to date. In this study, we introduced a rapid and convenient method that allows the investigation of fine vessel structures at a cellular level in the relatively intact retinal vasculature of adult zebrafish. Using an anti-ZO-1 antibody, tight junction structures in retinal vessels were examined in detail and several different cell types constituting blood vessels in arterial and capillary areas were identified. In addition, using cell type-specific antibodies, we identified smooth muscle cells, blood cells, and endothelial cells in the retinal vasculature. Finally, using the hyperglycemic model, we observed the dilation of retinal vessels, the downregulation of tight junction proteins, and the reduction in smooth muscle cells. Based on these results, we provide a rapid and convenient method for the study of retinal vasculature disease in the zebrafish animal model.<\/jats:p>","DOI":"10.3390\/ijms232315069","type":"journal-article","created":{"date-parts":[[2022,12,1]],"date-time":"2022-12-01T03:57:28Z","timestamp":1669867048000},"page":"15069","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Evaluation of a Rapid and Simple Method for Assessing Retinal Vessel Structures in Adult Zebrafish"],"prefix":"10.3390","volume":"23","author":[{"given":"Yu-Ri","family":"Lee","sequence":"first","affiliation":[{"name":"Korean Medicine Convergence Research Division, Korea Institute of Oriental Medicine (KIOM), Daejeon 34054, Republic of Korea"},{"name":"Department of Biology, Chungnam National University, Daejeon 34134, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9104-3991","authenticated-orcid":false,"given":"Myeongjoo","family":"Son","sequence":"additional","affiliation":[{"name":"Research Institute for Aerospace Medicine, Inha University, Incheon 22212, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5584-402X","authenticated-orcid":false,"given":"Young Sook","family":"Kim","sequence":"additional","affiliation":[{"name":"Korean Medicine Convergence Research Division, Korea Institute of Oriental Medicine (KIOM), Daejeon 34054, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin Sook","family":"Kim","sequence":"additional","affiliation":[{"name":"Korean Medicine Convergence Research Division, Korea Institute of Oriental Medicine (KIOM), Daejeon 34054, Republic of Korea"},{"name":"Julia Laboratory, Suwon 16232, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3019-0699","authenticated-orcid":false,"given":"Cheol-Hee","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Biology, Chungnam National University, Daejeon 34134, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9870-0923","authenticated-orcid":false,"given":"Seung-Hyun","family":"Jung","sequence":"additional","affiliation":[{"name":"Korean Medicine Convergence Research Division, Korea Institute of Oriental Medicine (KIOM), Daejeon 34054, Republic of Korea"},{"name":"Department of Genetic Resources, National Marine Biodiversity Institute of Korea (MABIK), Seocheon 33662, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,12,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Uhl, A., Busch, C., Marcel, S., and Veldhuis, R. 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