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CPD photolyase, a kind of DNA repair enzyme, can efficiently repair CPDs that are absent in humans and mice. Although exogenous CPD photolyases have beneficial effects on skin diseases, the mechanisms of CPD photolyases on the skin remain unknown. Here, this study prepared CPD photolyase nanoliposomes (CPDNL) from Antarctic Chlamydomonas sp. ICE-L, which thrives in harsh, high-UVB conditions, and evaluated their protective mechanisms against UVB-induced damage in mice. CPDNL were optimized using response surface methodology, characterized by a mean particle size of 105.5 nm, with an encapsulation efficiency of 63.3%. Topical application of CPDNL prevented UVB-induced erythema, epidermal thickness, and wrinkles in mice. CPDNL mitigated UVB-induced DNA damage by significantly decreasing the CPD concentration. CPDNL exhibited antioxidant properties as they reduced the production of reactive oxygen species (ROS) and malondialdehyde. Through activation of the NF-\u03baB pathway, CPDNL reduced the expression of pro-inflammatory cytokines including IL-6, TNF-\u03b1, and COX-2. Furthermore, CPDNL suppressed the MAPK signaling activation by downregulating the mRNA and protein expression of ERK, JNK, and p38 as well as AP-1. The MMP-1 and MMP-2 expressions were also remarkably decreased, which inhibited the collagen degradation. Therefore, we concluded that CPDNL exerted DNA repair, antioxidant, anti-inflammation, and anti-wrinkle properties as well as collagen protection via regulation of the NF-\u03baB\/MAPK\/MMP signaling pathways in UVB-induced mice, demonstrating that Antarctic CPD photolyases have the potential for skincare products against UVB and photoaging.<\/jats:p>","DOI":"10.3390\/ijms232315148","type":"journal-article","created":{"date-parts":[[2022,12,2]],"date-time":"2022-12-02T01:44:46Z","timestamp":1669945486000},"page":"15148","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Preparation of CPD Photolyase Nanoliposomes Derived from Antarctic Microalgae and Their Effect on UVB-Induced Skin Damage in Mice"],"prefix":"10.3390","volume":"23","author":[{"given":"Changfeng","family":"Qu","sequence":"first","affiliation":[{"name":"Key Laboratory of Marine Eco-Environmental Science and Technology, First Institute of Oceanography, Ministry of Natural Resources, Qingdao 266061, China"},{"name":"Laboratory for Marine Drugs and Bioproducts, Qingdao Pilot National Laboratory for Marine Science and Technology, Qingdao 266237, China"},{"name":"Marine Natural Products Research and Development Laboratory, Qingdao Key Laboratory, Qingdao 266061, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nianxu","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory of Marine Eco-Environmental Science and Technology, First Institute of Oceanography, Ministry of Natural Resources, Qingdao 266061, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tianlong","family":"Liu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Marine Eco-Environmental Science and Technology, First Institute of Oceanography, Ministry of Natural Resources, Qingdao 266061, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yingying","family":"He","sequence":"additional","affiliation":[{"name":"Key Laboratory of Marine Eco-Environmental Science and Technology, First Institute of Oceanography, Ministry of Natural Resources, Qingdao 266061, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jinlai","family":"Miao","sequence":"additional","affiliation":[{"name":"Key Laboratory of Marine Eco-Environmental Science and Technology, First Institute of Oceanography, Ministry of Natural Resources, Qingdao 266061, China"},{"name":"Laboratory for Marine Drugs and Bioproducts, Qingdao Pilot National Laboratory for Marine Science and Technology, Qingdao 266237, China"},{"name":"Marine Natural Products Research and Development Laboratory, Qingdao Key Laboratory, Qingdao 266061, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,12,2]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/j.mrfmmm.2004.06.057","article-title":"Mutations induced by ultraviolet light","volume":"571","author":"Pfeifer","year":"2005","journal-title":"Mutat. 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