{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T13:50:12Z","timestamp":1783000212563,"version":"3.54.5"},"reference-count":59,"publisher":"MDPI AG","issue":"23","license":[{"start":{"date-parts":[[2022,12,2]],"date-time":"2022-12-02T00:00:00Z","timestamp":1669939200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"James and Esther King Biomedical Research Program","award":["9JK08"],"award-info":[{"award-number":["9JK08"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Spontaneous intracerebral hemorrhage (sICH) is a disabling stroke sub-type, and tobacco use is a prominent risk factor for sICH. We showed that chronic nicotine exposure enhances bleeding post-sICH. Reduction of hematoma growth is a promising effective therapy for sICH in smoking subjects. Red-blood-cell-derived microparticles (RMPs) are hemostatic agents that limit hematoma expansion following sICH in na\u00efve rats. Considering the importance of testing the efficacy of experimental drugs in animal models with a risk factor for a disease, we tested RMP efficacy and the therapeutic time window in limiting hematoma growth post-sICH in rats exposed to nicotine. Young rats were chronically treated with nicotine using osmotic pumps. sICH was induced in rats using an injection of collagenase in the right striatum. Vehicle\/RMPs were administered intravenously. Hematoma volume and neurological impairment were quantified \u224824 h after sICH. Hematoma volumes in male and female nicotine-exposed rats that were treated with RMPs at 2 h post-sICH were significantly lower by 26 and 31% when compared to their respective control groups. RMP therapy was able to limit hematoma volume when administered up to 4.5 h post-sICH in animals of both sexes. Therefore, RMPs may limit hematoma growth in sICH patients exposed to tobacco use.<\/jats:p>","DOI":"10.3390\/ijms232315167","type":"journal-article","created":{"date-parts":[[2022,12,2]],"date-time":"2022-12-02T03:55:37Z","timestamp":1669953337000},"page":"15167","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Red Cell Microparticles Suppress Hematoma Growth Following Intracerebral Hemorrhage in Chronic Nicotine-Exposed Rats"],"prefix":"10.3390","volume":"23","author":[{"given":"Ashish K.","family":"Rehni","sequence":"first","affiliation":[{"name":"Peritz Scheinberg Cerebral Vascular Disease Research Laboratories, Miller School of Medicine, University of Miami, Miami, FL 33136, USA"},{"name":"Department of Neurology (D4-5), Miller School of Medicine, University of Miami, Miami, FL 33136, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sunjoo","family":"Cho","sequence":"additional","affiliation":[{"name":"Peritz Scheinberg Cerebral Vascular Disease Research Laboratories, Miller School of Medicine, University of Miami, Miami, FL 33136, USA"},{"name":"Department of Neurology (D4-5), Miller School of Medicine, University of Miami, Miami, FL 33136, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhexuan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering, University of Miami, Coral Gables, FL 33146, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Priyanka","family":"Khushal","sequence":"additional","affiliation":[{"name":"Peritz Scheinberg Cerebral Vascular Disease Research Laboratories, Miller School of Medicine, University of Miami, Miami, FL 33136, USA"},{"name":"Department of Neurology (D4-5), Miller School of Medicine, University of Miami, Miami, FL 33136, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1757-9947","authenticated-orcid":false,"given":"Ami P.","family":"Raval","sequence":"additional","affiliation":[{"name":"Peritz Scheinberg Cerebral Vascular Disease Research Laboratories, Miller School of Medicine, University of Miami, Miami, FL 33136, USA"},{"name":"Department of Neurology (D4-5), Miller School of Medicine, University of Miami, Miami, FL 33136, USA"},{"name":"Neuroscience Program, Miller School of Medicine, University of Miami, Miami, FL 33136, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sebastian","family":"Koch","sequence":"additional","affiliation":[{"name":"Department of Neurology (D4-5), Miller School of Medicine, University of Miami, Miami, FL 33136, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miguel A.","family":"Perez-Pinzon","sequence":"additional","affiliation":[{"name":"Peritz Scheinberg Cerebral Vascular Disease Research Laboratories, Miller School of Medicine, University of Miami, Miami, FL 33136, USA"},{"name":"Department of Neurology (D4-5), Miller School of Medicine, University of Miami, Miami, FL 33136, USA"},{"name":"Neuroscience Program, Miller School of Medicine, University of Miami, Miami, FL 33136, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weizhao","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Neurology (D4-5), Miller School of Medicine, University of Miami, Miami, FL 33136, USA"},{"name":"Department of Biomedical Engineering, University of Miami, Coral Gables, FL 33146, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenche","family":"Jy","sequence":"additional","affiliation":[{"name":"The Wallace H Coulter Platelet Laboratory, Division of Hematology\/Oncology, Department of Medicine, Miller School of Medicine, University of Miami, Miami, FL 33136, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kunjan R.","family":"Dave","sequence":"additional","affiliation":[{"name":"Peritz Scheinberg Cerebral Vascular Disease Research Laboratories, Miller School of Medicine, University of Miami, Miami, FL 33136, USA"},{"name":"Department of Neurology (D4-5), Miller School of Medicine, University of Miami, Miami, FL 33136, USA"},{"name":"Neuroscience Program, Miller School of Medicine, University of Miami, Miami, FL 33136, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,12,2]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2064","DOI":"10.1161\/STR.0b013e318296aeca","article-title":"An updated definition of stroke for the 21st century: A statement for healthcare professionals from the American Heart Association\/American Stroke Association","volume":"44","author":"Sacco","year":"2013","journal-title":"Stroke"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"795","DOI":"10.1016\/S1474-4422(21)00252-0","article-title":"Global, regional, and national burden of stroke and its risk factors, 1990-2019: A systematic analysis for the Global Burden of Disease Study 2019","volume":"20","author":"Feigin","year":"2021","journal-title":"Lancet Neurol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1175","DOI":"10.1212\/01.wnl.0000208408.98482.99","article-title":"Hematoma growth is a determinant of mortality and poor outcome after intracerebral hemorrhage","volume":"66","author":"Davis","year":"2006","journal-title":"Neurology"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1016\/S1474-4422(09)70340-0","article-title":"Incidence, case fatality, and functional outcome of intracerebral haemorrhage over time, according to age, sex, and ethnic origin: A systematic review and meta-analysis","volume":"9","author":"Luitse","year":"2010","journal-title":"Lancet Neurol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"37","DOI":"10.5853\/jos.2020.04770","article-title":"Tobacco Use: A Major Risk Factor of Intracerebral Hemorrhage","volume":"23","author":"Cho","year":"2021","journal-title":"J. Stroke"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"e005090","DOI":"10.1161\/JAHA.116.005090","article-title":"Age-Specific Vascular Risk Factor Profiles According to Stroke Subtype","volume":"6","author":"Hauer","year":"2017","journal-title":"J. Am. Heart Assoc."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"e003540","DOI":"10.1161\/JAHA.116.003540","article-title":"Race-Specific Predictors of Mortality in Intracerebral Hemorrhage: Differential Impacts of Intraventricular Hemorrhage and Age Among Blacks and Whites","volume":"5","author":"Faigle","year":"2016","journal-title":"J. Am. Heart Assoc."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Rehni, A.K., Cho, S., Zhang, Z., Zhao, W., Raval, A.P., Perez-Pinzon, M.A., and Dave, K.R. (2022). Chronic Nicotine Exposure Increases Hematoma Expansion following Collagenase-Induced Intracerebral Hemorrhage in Rats. Biomolecules, 12.","DOI":"10.3390\/biom12050621"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"647","DOI":"10.1161\/STROKEAHA.114.007704","article-title":"Time course and predictors of neurological deterioration after intracerebral hemorrhage","volume":"46","author":"Lord","year":"2015","journal-title":"Stroke"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"457","DOI":"10.1111\/j.1747-4949.2010.00487.x","article-title":"Novel approaches to the treatment of intracerebral haemorrhage","volume":"5","author":"Staykov","year":"2010","journal-title":"Int. J. Stroke"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1493","DOI":"10.1001\/jamaneurol.2019.2636","article-title":"Effect of Recombinant Activated Coagulation Factor VII on Hemorrhage Expansion Among Patients With Spot Sign-Positive Acute Intracerebral Hemorrhage: The SPOTLIGHT and STOP-IT Randomized Clinical Trials","volume":"76","author":"Gladstone","year":"2019","journal-title":"JAMA Neurol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"2605","DOI":"10.1016\/S0140-6736(16)30392-0","article-title":"Platelet transfusion versus standard care after acute stroke due to spontaneous cerebral haemorrhage associated with antiplatelet therapy (PATCH): A randomised, open-label, phase 3 trial","volume":"387","author":"Baharoglu","year":"2016","journal-title":"Lancet"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1812","DOI":"10.1161\/STROKEAHA.112.652065","article-title":"Poor prognosis in warfarin-associated intracranial hemorrhage despite anticoagulation reversal","volume":"43","author":"Dowlatshahi","year":"2012","journal-title":"Stroke"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1007\/s11936-013-0277-y","article-title":"New avenues for treatment of intracranial hemorrhage","volume":"16","author":"Sonni","year":"2014","journal-title":"Curr. Treat. Options Cardiovasc. Med."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"751","DOI":"10.1160\/TH12-12-0941","article-title":"Red cell-derived microparticles (RMP) as haemostatic agent","volume":"110","author":"Jy","year":"2013","journal-title":"Thromb. Haemost."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"639","DOI":"10.1055\/s-0037-1615646","article-title":"Cell-derived microparticles circulate in healthy humans and support low grade thrombin generation","volume":"85","author":"Berckmans","year":"2001","journal-title":"Thromb. Haemost."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"7171","DOI":"10.1016\/S0021-9258(18)53161-6","article-title":"Contribution of platelet microparticle formation and granule secretion to the transmembrane migration of phosphatidylserine","volume":"268","author":"Chang","year":"1993","journal-title":"J. Biol. Chem."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"3182","DOI":"10.1161\/STROKEAHA.122.039641","article-title":"Red Blood Cell Microparticles Limit Hematoma Growth in Intracerebral Hemorrhage","volume":"53","author":"Rehni","year":"2022","journal-title":"Stroke"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1036","DOI":"10.1093\/ije\/dyi104","article-title":"Smoking, quitting, and the risk of cardiovascular disease among women and men in the Asia-Pacific region","volume":"34","author":"Woodward","year":"2005","journal-title":"Int. J. Epidemiol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"917","DOI":"10.1586\/erc.10.56","article-title":"Smoking and stroke: The more you smoke the more you stroke","volume":"8","author":"Shah","year":"2010","journal-title":"Expert Rev. Cardiovasc. Ther."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"516","DOI":"10.1038\/sj.jcbfm.9600548","article-title":"Intracerebral hemorrhage models in rat: Comparing collagenase to blood infusion","volume":"28","author":"MacLellan","year":"2008","journal-title":"J. Cereb. Blood Flow Metab."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"42","DOI":"10.5853\/jos.2018.03125","article-title":"Executive Summary of Stroke Statistics in Korea 2018: A Report from the Epidemiology Research Council of the Korean Stroke Society","volume":"21","author":"Kim","year":"2019","journal-title":"J. Stroke"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"2355","DOI":"10.1056\/NEJMoa1214609","article-title":"Rapid blood-pressure lowering in patients with acute intracerebral hemorrhage","volume":"368","author":"Anderson","year":"2013","journal-title":"N. Engl. J. Med."},{"key":"ref_24","unstructured":"Institute of Health Metrics (2021, July 17). Global Burden of Disease [database]. Washington DC. Available online: https:\/\/www.who.int\/news-room\/fact-sheets\/detail\/tobacco."},{"key":"ref_25","unstructured":"National Center for Health Statistics (2022, January 13). Electronic Cigarette Use Among U.S. Adults, Available online: https:\/\/www.cdc.gov\/nchs\/products\/databriefs\/db365.htm."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"447","DOI":"10.1038\/nrcardio.2017.36","article-title":"Cardiovascular effects of electronic cigarettes","volume":"14","author":"Benowitz","year":"2017","journal-title":"Nat. Rev. Cardiol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1016\/j.redox.2017.06.006","article-title":"Offsetting the impact of smoking and e-cigarette vaping on the cerebrovascular system and stroke injury: Is Metformin a viable countermeasure?","volume":"13","author":"Kaisar","year":"2017","journal-title":"Redox Biol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"512","DOI":"10.1055\/s-0039-3402451","article-title":"An Update on Biological and Clinical Associations between E-Cigarettes and Myocardial Infarction","volume":"46","author":"Lippi","year":"2020","journal-title":"Semin. Thromb. Hemost."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"e254","DOI":"10.1161\/CIR.0000000000000950","article-title":"Heart Disease and Stroke Statistics-2021 Update: A Report From the American Heart Association","volume":"143","author":"Virani","year":"2021","journal-title":"Circulation"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"987","DOI":"10.1161\/01.STR.24.7.987","article-title":"Volume of intracerebral hemorrhage. A powerful and easy-to-use predictor of 30-day mortality","volume":"24","author":"Broderick","year":"1993","journal-title":"Stroke"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"2177","DOI":"10.1016\/j.jstrokecerebrovasdis.2019.04.038","article-title":"Hematoma Expansion Predictors: Laboratory and Radiological Risk Factors in Patients with Acute Intracerebral Hemorrhage: A Prospective Observational Study","volume":"28","author":"Elkhatib","year":"2019","journal-title":"J. Stroke Cerebrovasc. Dis."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"599","DOI":"10.3389\/fphar.2018.00599","article-title":"Pharmacokinetics of Human Red Blood Cell Microparticles Prepared Using High-Pressure Extrusion Method","volume":"9","author":"Jy","year":"2018","journal-title":"Front. Pharmacol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"2244","DOI":"10.1161\/STROKEAHA.108.541128","article-title":"Update of the stroke therapy academic industry roundtable preclinical recommendations","volume":"40","author":"Fisher","year":"2009","journal-title":"Stroke"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1159","DOI":"10.1016\/0735-1097(93)90431-Y","article-title":"Nicotine effects on eicosanoid formation and hemostatic function: Comparison of transdermal nicotine and cigarette smoking","volume":"22","author":"Benowitz","year":"1993","journal-title":"J. Am. Coll. Cardiol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"450","DOI":"10.1038\/236450a0","article-title":"Smoking, platelets and thrombosis","volume":"236","author":"Hawkins","year":"1972","journal-title":"Nature"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"619","DOI":"10.1161\/01.CIR.48.3.619","article-title":"An acute effect of cigarette smoking on platelet function. A possible link between smoking and arterial thrombosis","volume":"48","author":"Levine","year":"1973","journal-title":"Circulation"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"389","DOI":"10.1038\/clpt.1984.193","article-title":"Platelet function after cigarette smoking in relation to nicotine and carbon monoxide","volume":"36","author":"Renaud","year":"1984","journal-title":"Clin. Pharmacol. Ther."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1019","DOI":"10.1080\/15287394.2015.1043607","article-title":"Oxidative Stress Induced by Cigarette Smoke Extracts in Human Brain Cells (T98G) and Human Brain Microvascular Endothelial Cells (HBMEC) in Mono- and Co-Culture","volume":"78","author":"Kim","year":"2015","journal-title":"J. Toxicol. Environ. Health A"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"43","DOI":"10.3109\/15563657508988045","article-title":"Alkaloids of tobacco and blood coagulation: Effect of nicotine on thrombin and fibrinogen","volume":"8","author":"Singh","year":"1975","journal-title":"Clin. Toxicol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"474","DOI":"10.1177\/1074248419838512","article-title":"Preclinical Evaluation of Safety and Biodistribution of Red Cell Microparticles: A Novel Hemostatic Agent","volume":"24","author":"Rehni","year":"2019","journal-title":"J. Cardiovasc. Pharmacol. Ther."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1783","DOI":"10.1161\/01.STR.27.10.1783","article-title":"Enlargement of spontaneous intracerebral hemorrhage. Incidence and time course","volume":"27","author":"Kazui","year":"1996","journal-title":"Stroke"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"2127","DOI":"10.1056\/NEJMoa0707534","article-title":"Efficacy and safety of recombinant activated factor VII for acute intracerebral hemorrhage","volume":"358","author":"Mayer","year":"2008","journal-title":"N. Engl. J. Med."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1159\/000215941","article-title":"Early hospital arrival improves outcome at discharge in ischemic but not hemorrhagic stroke: A prospective multicenter study","volume":"28","author":"Naganuma","year":"2009","journal-title":"Cerebrovasc. Dis."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1111\/ane.12359","article-title":"Lower hazard ratio for death in women with cerebral hemorrhage","volume":"132","author":"Shigematsu","year":"2015","journal-title":"Acta Neurol. Scand."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1016\/j.jns.2017.05.057","article-title":"Sex differences in intracerebral hemorrhage expansion and mortality","volume":"379","author":"Marini","year":"2017","journal-title":"J. Neurol. Sci."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"380","DOI":"10.1089\/jwh.2016.5849","article-title":"Sex and Age Interactions and Differences in Outcomes After Intracerebral Hemorrhage","volume":"26","author":"James","year":"2017","journal-title":"J. Womens Health"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"219097","DOI":"10.1155\/2013\/219097","article-title":"Female gender remains an independent risk factor for poor outcome after acute nontraumatic intracerebral hemorrhage","volume":"2013","author":"Ganti","year":"2013","journal-title":"Neurol. Res. Int."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"487","DOI":"10.1097\/00004647-200405000-00002","article-title":"Intracerebral hemorrhage in mice: Model characterization and application for genetically modified mice","volume":"24","author":"Nakamura","year":"2004","journal-title":"J. Cereb. Blood Flow Metab."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"107","DOI":"10.2165\/00003088-200342020-00001","article-title":"The influence of sex on pharmacokinetics","volume":"42","author":"Schwartz","year":"2003","journal-title":"Clin. Pharm."},{"key":"ref_50","first-page":"1254","article-title":"Sex-based differences in drug activity","volume":"80","author":"Whitley","year":"2009","journal-title":"Am. Fam. Physician"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"644","DOI":"10.1007\/s12325-019-01201-3","article-title":"Sex-Related Differences in Pharmacokinetics and Pharmacodynamics of Frequently Prescribed Drugs: A Review of the Literature","volume":"37","author":"Farkouh","year":"2020","journal-title":"Adv. Ther."},{"key":"ref_52","first-page":"708","article-title":"Women\u2019s involvement in clinical trials: Historical perspective and future implications","volume":"14","author":"Liu","year":"2016","journal-title":"Pharm. Pract."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"1699","DOI":"10.1016\/0024-3205(87)90020-8","article-title":"Nicotine administration to rats: Methodological considerations","volume":"40","author":"Murrin","year":"1987","journal-title":"Life Sci."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"216","DOI":"10.1016\/j.neuroscience.2011.02.036","article-title":"Nicotine and estrogen synergistically exacerbate cerebral ischemic injury","volume":"181","author":"Raval","year":"2011","journal-title":"Neuroscience"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1097\/00004647-199702000-00002","article-title":"Chronic nicotine treatment enhances focal ischemic brain injury and depletes free pool of brain microvascular tissue plasminogen activator in rats","volume":"17","author":"Wang","year":"1997","journal-title":"J. Cereb. Blood Flow Metab."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1016\/j.neuroscience.2009.02.065","article-title":"Pretreatment with a single estradiol-17beta bolus activates cyclic-AMP response element binding protein and protects CA1 neurons against global cerebral ischemia","volume":"160","author":"Raval","year":"2009","journal-title":"Neuroscience"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"789","DOI":"10.1007\/s12975-019-00754-3","article-title":"Automated Assessment of Hematoma Volume of Rodents Subjected to Experimental Intracerebral Hemorrhagic Stroke by Bayes Segmentation Approach","volume":"11","author":"Zhang","year":"2020","journal-title":"Transl. Stroke Res."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"1616","DOI":"10.1161\/01.STR.27.9.1616","article-title":"Middle cerebral artery occlusion in the rat by intraluminal suture. Neurological and pathological evaluation of an improved model","volume":"27","author":"Belayev","year":"1996","journal-title":"Stroke"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"1112","DOI":"10.1161\/01.STR.27.6.1112","article-title":"Depiction of infarct frequency distribution by computer-assisted image mapping in rat brains with middle cerebral artery occlusion. Comparison of photothrombotic and intraluminal suture models","volume":"27","author":"Zhao","year":"1996","journal-title":"Stroke"}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/23\/23\/15167\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T01:32:42Z","timestamp":1760146362000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/23\/23\/15167"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,12,2]]},"references-count":59,"journal-issue":{"issue":"23","published-online":{"date-parts":[[2022,12]]}},"alternative-id":["ijms232315167"],"URL":"https:\/\/doi.org\/10.3390\/ijms232315167","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,12,2]]}}}