{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T09:45:31Z","timestamp":1789033531790,"version":"build-2803163510"},"reference-count":70,"publisher":"MDPI AG","issue":"23","license":[{"start":{"date-parts":[[2022,12,2]],"date-time":"2022-12-02T00:00:00Z","timestamp":1669939200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"European Regional Development Fund-cofinanced grant","award":["PID2019-103924GB-I00"],"award-info":[{"award-number":["PID2019-103924GB-I00"]}]},{"name":"European Regional Development Fund-cofinanced grant","award":["P18-FR-1359"],"award-info":[{"award-number":["P18-FR-1359"]}]},{"name":"Plan Andaluz de Investigaci\u00f3n, Desarrollo e Innovaci\u00f3n","award":["PID2019-103924GB-I00"],"award-info":[{"award-number":["PID2019-103924GB-I00"]}]},{"name":"Plan Andaluz de Investigaci\u00f3n, Desarrollo e Innovaci\u00f3n","award":["P18-FR-1359"],"award-info":[{"award-number":["P18-FR-1359"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Lipoxygenases (LOXs) catalyze the insertion of molecular oxygen into polyunsaturated fatty acids (PUFA) such as linoleic and linolenic acids, being the first step in the biosynthesis of a large group of biologically active fatty acid (FA)-derived metabolites collectively named oxylipins. LOXs are involved in multiple functions such as the biosynthesis of jasmonic acid (JA) and volatile molecules related to the aroma and flavor production of plant tissues, among others. Using sweet pepper (Capsicum annuum L.) plants as a model, LOX activity was assayed by non-denaturing polyacrylamide gel electrophoresis (PAGE) and specific in-gel activity staining. Thus, we identified a total of seven LOX isozymes (I to VII) distributed among the main plant organs (roots, stems, leaves, and fruits). Furthermore, we studied the FA profile and the LOX isozyme pattern in pepper fruits including a sweet variety (Melchor) and three autochthonous Spanish varieties that have different pungency levels (Piquillo, Padr\u00f3n, and Alegr\u00eda riojana). It was observed that the number of LOX isozymes increased as the capsaicin content increased in the fruits. On the other hand, a total of eight CaLOX genes were identified in sweet pepper fruits, and their expression was differentially regulated during ripening and by the treatment with nitric oxide (NO) gas. Finally, a deeper analysis of the LOX IV isoenzyme activity in the presence of nitrosocysteine (CysNO, a NO donor) suggests a regulatory mechanism via S-nitrosation. In summary, our data indicate that the different LOX isozymes are differentially regulated by the capsaicin content, fruit ripening, and NO.<\/jats:p>","DOI":"10.3390\/ijms232315211","type":"journal-article","created":{"date-parts":[[2022,12,5]],"date-time":"2022-12-05T01:42:21Z","timestamp":1670204541000},"page":"15211","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Lipoxygenase (LOX) in Sweet and Hot Pepper (Capsicum annuum L.) Fruits during Ripening and under an Enriched Nitric Oxide (NO) Gas Atmosphere"],"prefix":"10.3390","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3678-5503","authenticated-orcid":false,"given":"Salvador","family":"Gonz\u00e1lez-Gordo","sequence":"first","affiliation":[{"name":"Group of Antioxidants, Free Radicals and Nitric Oxide in Biotechnology, Food and Agriculture, Department of Stress, Development and Signaling in Plants, Estaci\u00f3n Experimental del Zaid\u00edn, Spanish National Research Council (CSIC), Profesor Albareda 1, E-18008 Granada, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amanda","family":"Ca\u00f1as","sequence":"additional","affiliation":[{"name":"Group of Antioxidants, Free Radicals and Nitric Oxide in Biotechnology, Food and Agriculture, Department of Stress, Development and Signaling in Plants, Estaci\u00f3n Experimental del Zaid\u00edn, Spanish National Research Council (CSIC), Profesor Albareda 1, E-18008 Granada, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda A.","family":"Mu\u00f1oz-Vargas","sequence":"additional","affiliation":[{"name":"Group of Antioxidants, Free Radicals and Nitric Oxide in Biotechnology, Food and Agriculture, Department of Stress, Development and Signaling in Plants, Estaci\u00f3n Experimental del Zaid\u00edn, Spanish National Research Council (CSIC), Profesor Albareda 1, E-18008 Granada, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6673-3571","authenticated-orcid":false,"given":"Jos\u00e9 M.","family":"Palma","sequence":"additional","affiliation":[{"name":"Group of Antioxidants, Free Radicals and Nitric Oxide in Biotechnology, Food and Agriculture, Department of Stress, Development and Signaling in Plants, Estaci\u00f3n Experimental del Zaid\u00edn, Spanish National Research Council (CSIC), Profesor Albareda 1, E-18008 Granada, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1814-9212","authenticated-orcid":false,"given":"Francisco J.","family":"Corpas","sequence":"additional","affiliation":[{"name":"Group of Antioxidants, Free Radicals and Nitric Oxide in Biotechnology, Food and Agriculture, Department of Stress, Development and Signaling in Plants, Estaci\u00f3n Experimental del Zaid\u00edn, Spanish National Research Council (CSIC), Profesor Albareda 1, E-18008 Granada, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,12,2]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"3151","DOI":"10.1021\/jf060022q","article-title":"Lipoxygenase from banana leaf: Purification and characterization of an enzyme that catalyzes linoleic acid oxygenation at the 9-position","volume":"54","author":"Kuo","year":"2006","journal-title":"J. Agric. Food Chem."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1146\/annurev.arplant.53.100301.135248","article-title":"The lipoxygenase pathway","volume":"53","author":"Feussner","year":"2002","journal-title":"Annu. Rev. Plant Biol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"348","DOI":"10.1016\/j.jplph.2005.11.006","article-title":"Lipoxygenases: Occurrence, functions and catalysis","volume":"163","author":"Liavonchanka","year":"2006","journal-title":"J. Plant Physiol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"6155","DOI":"10.1073\/pnas.97.11.6155","article-title":"Direct activation of capsaicin receptors by products of lipoxygenases: Endogenous capsaicin-like substances","volume":"97","author":"Hwang","year":"2000","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_5","first-page":"5","article-title":"Lipoxygenases and their metabolites in formation of plant stress tolerance","volume":"89","author":"Babenko","year":"2017","journal-title":"Ukr. Biochem. J."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1007\/s12374-020-09241-x","article-title":"Plant lipoxygenases and their role in plant physiology","volume":"63","author":"Viswanath","year":"2020","journal-title":"J. Plant Biol."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Singh, P., Arif, Y., Miszczuk, E., Bajguz, A., and Hayat, S. (2022). Specific roles of lipoxygenases in development and responses to stress in plants. Plants, 11.","DOI":"10.3390\/plants11070979"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"401","DOI":"10.1007\/s12013-020-00947-w","article-title":"Lipids composition in plant membranes","volume":"78","author":"Hanaka","year":"2020","journal-title":"Cell Biochem. Biophys."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"100231","DOI":"10.1016\/j.xplc.2021.100231","article-title":"Metabolism, signaling, and transport of jasmonates","volume":"2","author":"Li","year":"2021","journal-title":"Plant Commun."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1016\/j.plaphy.2010.02.004","article-title":"Exploring the impact of wounding and jasmonates on ascorbate metabolism","volume":"48","author":"Suza","year":"2010","journal-title":"Plant Physiol. Biochem."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"390","DOI":"10.3389\/fpls.2020.00390","article-title":"Plant Unsaturated fatty acids: Biosynthesis and regulation","volume":"11","author":"He","year":"2020","journal-title":"Front. Plant Sci."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1771","DOI":"10.3389\/fpls.2018.01771","article-title":"Abiotic stresses: General defenses of land plants and chances for engineering multistress tolerance","volume":"9","author":"He","year":"2018","journal-title":"Front. Plant Sci."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Shramko, V.S., Polonskaya, Y.V., Kashtanova, E.V., Stakhneva, E.M., and Ragino, Y.I. (2020). The short overview on the relevance of fatty acids for human cardiovascular disorders. Biomolecules, 10.","DOI":"10.3390\/biom10081127"},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Guevara, L., Dom\u00ednguez-Anaya, M.A., Ortigosa, A., Gonz\u00e1lez-Gordo, S., D\u00edaz, C., Vicente, F., Corpas, F.J., P\u00e9rez del Palacio, J., and Palma, J.M. (2021). Identification of compounds with potential therapeutic uses from sweet pepper (Capsicum annuum L.) fruits and their modulation by nitric oxide (NO). Int. J. Mol. Sci., 22.","DOI":"10.20944\/preprints202103.0675.v1"},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Batiha, G.E., Alqahtani, A., Ojo, O.A., Shaheen, H.M., Wasef, L., Elzeiny, M., Ismail, M., Shalaby, M., Murata, T., and Zaragoza-Bastida, A. (2020). Biological properties, bioactive constituents, and pharmacokinetics of some Capsicum spp. and Capsaicinoids. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21155179"},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Chu-Puga, \u00c1., Gonz\u00e1lez-Gordo, S., Rodr\u00edguez-Ruiz, M., Palma, J.M., and Corpas, F.J. (2019). NADPH oxidase (Rboh) activity is up regulated during sweet pepper (Capsicum annuum L.) fruit ripening. Antioxidants, 8.","DOI":"10.3390\/antiox8010009"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1016\/j.niox.2018.10.002","article-title":"Endogenous hydrogen sulfide (H2S) is up-regulated during sweet pepper (Capsicum annuum L.) fruit ripening. In vitro analysis shows that NADP-dependent isocitrate dehydrogenase (ICDH) activity is inhibited by H2S and NO","volume":"81","author":"Palma","year":"2018","journal-title":"Nitric Oxide"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"278","DOI":"10.1111\/ppl.13000","article-title":"Inhibition of NADP-malic enzyme activity by H2S and NO in sweet pepper (Capsicum annuum L.) fruits","volume":"168","author":"Palma","year":"2020","journal-title":"Physiol. Plant"},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Rodr\u00edguez-Ruiz, M., Gonz\u00e1lez-Gordo, S., Ca\u00f1as, A., Campos, M.J., Paradela, A., Corpas, F.J., and Palma, J.M. (2019). Sweet pepper (Capsicum annuum L.) fruits contain an atypical peroxisomal catalase that is modulated by reactive oxygen and nitrogen species. Antioxidants, 8.","DOI":"10.3390\/antiox8090374"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1016\/j.redox.2017.02.009","article-title":"Characterization of the galactono-1,4-lactone dehydrogenase from pepper fruits and its modulation in the ascorbate biosynthesis. Role of nitric oxide","volume":"12","author":"Mateos","year":"2017","journal-title":"Redox Biol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"4557","DOI":"10.1093\/jxb\/erz136","article-title":"Nitric oxide-dependent regulation of sweet pepper fruit ripening","volume":"70","author":"Bautista","year":"2019","journal-title":"J. Exp. Bot."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"485","DOI":"10.3389\/fpls.2020.00485","article-title":"Superoxide radical metabolism in sweet pepper (Capsicum annuum L.) fruits is regulated by ripening and by a NO-enriched environment","volume":"11","author":"Palma","year":"2020","journal-title":"Front. Plant Sci."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Gonz\u00e1lez-Gordo, S., Rodr\u00edguez-Ruiz, M., L\u00f3pez-Jaramillo, J., Mu\u00f1oz-Vargas, M.A., Palma, J.M., and Corpas, F.J. (2022). Nitric oxide (NO) Differentially modulates the ascorbate peroxidase (APX) isozymes of sweet pepper (Capsicum annuum L.) fruits. Antioxidants, 11.","DOI":"10.3390\/antiox11040765"},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Palma, J.M., Ter\u00e1n, F., Contreras-Ruiz, A., Rodr\u00edguez-Ruiz, M., and Corpas, F.J. (2020). Antioxidant profile of pepper (Capsicum annuum L.) fruits containing diverse levels of capsaicinoids. Antioxidants, 9.","DOI":"10.20944\/preprints202008.0010.v1"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"941","DOI":"10.1093\/jxb\/eraa526","article-title":"Multifaceted roles of nitric oxide in tomato fruit ripening: NO-induced metabolic rewiring and consequences for fruit quality traits","volume":"72","author":"Zuccarelli","year":"2021","journal-title":"J. Exp. Bot."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1016\/j.postharvbio.2006.05.004","article-title":"Inhibition by nitric oxide of ethylene biosynthesis and lipoxygenase activity in peach fruit during storage","volume":"42","author":"Zhu","year":"2006","journal-title":"Postharvest Biol. Technol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1104\/pp.010787","article-title":"Plant lipoxygenases. Physiological and molecular features","volume":"130","author":"Porta","year":"2002","journal-title":"Plant Physiol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"363","DOI":"10.1007\/s00425-007-0623-y","article-title":"Wounding and pathogen infection induce a chloroplast-targeted lipoxygenase in the common bean (Phaseolus vulgaris L.)","volume":"227","author":"Porta","year":"2008","journal-title":"Planta"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"400","DOI":"10.1016\/j.plantsci.2006.10.002","article-title":"Involvement of phospholipase D and lipoxygenase in response to chilling stress in postharvest cucumber fruits","volume":"172","author":"Mao","year":"2007","journal-title":"Plant Sci."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"2481","DOI":"10.3390\/ijms13022481","article-title":"The expression profiling of the lipoxygenase (LOX) family genes during fruit development, abiotic stress and hormonal treatments in cucumber (Cucumis sativus L.)","volume":"13","author":"Yang","year":"2012","journal-title":"Int. J. Mol. Sci."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Meng, Y., Liang, Y., Liao, B., He, W., Liu, Q., Shen, X., Xu, J., and Chen, S. (2022). Genome-wide identification, characterization and expression analysis of lipoxygenase gene family in Artemisia annua L.. Plants, 11.","DOI":"10.3390\/plants11050655"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1016\/0168-9452(92)90145-C","article-title":"Appearance of new lipoxygenase isozymes during seedling germination of Vicia sativa","volume":"87","author":"Andrianarison","year":"1992","journal-title":"Plant Sci."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"535","DOI":"10.1104\/pp.107.2.535","article-title":"The Lipoxygenase isozymes in soybean [Glycine max (L.) Merr.] leaves (changes during leaf development, after wounding, and following reproductive sink removal)","volume":"107","author":"Saravitz","year":"1995","journal-title":"Plant Physiol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"e12438","DOI":"10.1111\/jfbc.12438","article-title":"Lipoxygenase activity associated to fruit ripening and senescence in chayote (Sechium edule Jacq. Sw. cv. \u201cvirens levis\u201d)","volume":"42","year":"2018","journal-title":"J. Food Biochem."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/j.postharvbio.2006.06.012","article-title":"The relationship between water loss, lipid content, membrane integrity and LOX activity in ripe pepper fruit after storage","volume":"42","author":"Maalekuua","year":"2006","journal-title":"Postharvest Biol. Technol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"491","DOI":"10.1016\/j.enzmictec.2006.11.025","article-title":"Lipoxygenase in fruits and vegetables: A review","volume":"40","author":"Baysal","year":"2007","journal-title":"Enzym. Microb. Technol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"2641","DOI":"10.1104\/pp.104.041608","article-title":"Identification of a specific isoform of tomato lipoxygenase (TomloxC) involved in the generation of fatty acid-derived flavor compounds","volume":"136","author":"Chen","year":"2004","journal-title":"Plant Physiol."},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Huang, D., Ma, F., Wu, B., Lv, W., Xu, Y., Xing, W., Chen, D., Xu, B., and Song, S. (2022). Genome-wide association and expression analysis of the lipoxygenase gene family in Passiflora edulis revealing PeLOX4 might be involved in fruit ripeness and ester formation. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms232012496"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"318","DOI":"10.1016\/j.jplph.2018.10.001","article-title":"Genome-wide identification of tomato (Solanum lycopersicum L.) lipoxygenases coupled with expression profiles during plant development and in response to methyl-jasmonate and wounding","volume":"231","author":"Upadhyay","year":"2018","journal-title":"J. Plant Physiol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1104\/pp.106.1.109","article-title":"The cloning of two tomato lipoxygenase genes and their differential expression during fruit ripening","volume":"106","author":"Ferrie","year":"1994","journal-title":"Plant Physiol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"419","DOI":"10.1093\/jxb\/ert382","article-title":"A 13-lipoxygenase, TomloxC, is essential for synthesis of C5 flavour volatiles in tomato","volume":"65","author":"Shen","year":"2014","journal-title":"J. Exp. Bot."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"793","DOI":"10.1093\/jxb\/50.335.793","article-title":"Ethylene and developmental signals regulate expression of lipoxygenase genes during tomato fruit ripening","volume":"50","author":"Griffiths","year":"1999","journal-title":"J. Exp. Bot."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"1899","DOI":"10.1104\/pp.106.093765","article-title":"A genomics approach reveals that aroma production in apple is controlled by ethylene predominantly at the final step in each biosynthetic pathway","volume":"144","author":"Schaffer","year":"2007","journal-title":"Plant Physiol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"375","DOI":"10.1007\/s11103-018-0785-y","article-title":"Genome-wide identification, classification and expression of lipoxygenase gene family in pepper","volume":"98","author":"Sarde","year":"2018","journal-title":"Plant Mol. Biol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"363","DOI":"10.1146\/annurev-arplant-042817-040440","article-title":"The Oxylipin Pathways: Biochemistry and Function","volume":"69","author":"Wasternack","year":"2018","journal-title":"Annu. Rev. Plant Biol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"942789","DOI":"10.3389\/fpls.2022.942789","article-title":"Jasmonates in plant growth and development and elicitation of secondary metabolites: An updated overview","volume":"13","author":"Sohn","year":"2022","journal-title":"Front. Plant Sci."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"948","DOI":"10.1104\/pp.109.147827","article-title":"The pepper 9-lipoxygenase gene CaLOX1 functions in defense and cell death responses to microbial pathogens","volume":"152","author":"Hwang","year":"2010","journal-title":"Plant Physiol."},{"key":"ref_48","first-page":"23","article-title":"Biosynthesis of acyl moieties of capsaicin and its analogues from valine and leucine in Capsicum fruits","volume":"22","author":"Suzuki","year":"1981","journal-title":"Plant Cell Physiol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1016\/S1466-8564(01)00022-4","article-title":"Phenylalanine ammonia-lyase and antioxidant activities of lipophilic fraction of fresh pepper fruits Capsicum annum L.","volume":"2","author":"Perucka","year":"2001","journal-title":"Innov. Food Sci. Emerg. Technol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1007\/s13765-012-1163-0","article-title":"Metabolite analysis of long chain branched fatty acids and capsaicin biosynthesis in Capsicum annuum placenta","volume":"55","author":"Keum","year":"2012","journal-title":"J. Korean Soc. Appl. Biol. Chem."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"34121","DOI":"10.1038\/srep34121","article-title":"Discovery of putative capsaicin biosynthetic genes by RNA-Seq and digital gene expression analysis of pepper","volume":"6","author":"Zhang","year":"2016","journal-title":"Sci. Rep."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"371","DOI":"10.1111\/j.1399-3054.2012.01684.x","article-title":"Proteomic analysis of S-nitrosylated proteins in potato plant","volume":"148","author":"Kato","year":"2013","journal-title":"Physiol. Plant."},{"key":"ref_53","doi-asserted-by":"crossref","unstructured":"Ul Haq, A., Lateef Lone, M., Farooq, S., Parveen, S., Altaf, F., Tahir, I., Ingo Hefft, D., Ahmad, A., and Ahmad, P. (2021). Nitric oxide effectively orchestrates postharvest flower senescence: A case study of Consolida ajacis. Funct. Plant Biol, ahead of print.","DOI":"10.1071\/FP21241"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1093\/jxb\/erm345","article-title":"Nitric oxide-responsive genes and promoters in Arabidopsis thaliana: A bioinformatics approach","volume":"59","author":"Palmieri","year":"2008","journal-title":"J. Exp. Bot."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"31","DOI":"10.17660\/ActaHortic.2020.1275.5","article-title":"Regulation of nitric oxide on lipoxygenase in peach fruit during cold storage","volume":"1275","author":"Zhang","year":"2020","journal-title":"Acta Hortic."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"4405","DOI":"10.1093\/jxb\/erz350","article-title":"Nitric oxide in the physiology and quality of fleshy fruits","volume":"70","author":"Palma","year":"2019","journal-title":"J. Exp. Bot."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1111\/j.1365-3040.2011.02310.x","article-title":"Metabolism of reactive oxygen species and reactive nitrogen species in pepper (Capsicum annuum L.) plants under low temperature stress","volume":"35","author":"Airaki","year":"2012","journal-title":"Plant Cell Environ."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"679","DOI":"10.1093\/aob\/mcv023","article-title":"Spatial and temporal regulation of the metabolism of reactive oxygen and nitrogen species during the early development of pepper (Capsicum annuum) seedlings","volume":"116","author":"Airaki","year":"2015","journal-title":"Ann. Bot."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1007\/978-1-4939-7695-9_1","article-title":"A simple and useful method to apply exogenous NO gas to plant systems: Bell pepper fruits as a model","volume":"1747","author":"Palma","year":"2018","journal-title":"Methods Mol. Biol."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1002\/lipi.19960980507","article-title":"Staining for soybean lipoxygenase activity in electrophoretic gels","volume":"98","author":"Heinisch","year":"1996","journal-title":"Lipid\/Fett"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"W585","DOI":"10.1093\/nar\/gkm259","article-title":"WoLF PSORT: Protein localization predictor","volume":"35","author":"Horton","year":"2007","journal-title":"Nucleic Acids Res."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"429","DOI":"10.1007\/978-1-59745-466-7_29","article-title":"Computational prediction of subcellular localization","volume":"390","author":"Nakai","year":"2007","journal-title":"Methods Mol. Biol."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"D607","DOI":"10.1093\/nar\/gky1131","article-title":"2019. STRING v11: Protein-protein association networks with increased coverage, supporting functional discovery in genome-wide experimental datasets","volume":"47","author":"Szklarczyk","year":"2019","journal-title":"Nucleic Acids Res."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1007\/s11745-008-3245-7","article-title":"Diversity of the enzymatic activity in the lipoxygenase gene family of Arabidopsis thaliana","volume":"4","author":"Bannenberg","year":"2009","journal-title":"Lipids"},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"2030","DOI":"10.1016\/j.phytochem.2006.06.023","article-title":"Lipoxygenases during Brassica napus seed germination","volume":"67","author":"Terp","year":"2006","journal-title":"Phytochemistry"},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"42","DOI":"10.3389\/fpls.2012.00042","article-title":"Oxylipin Signaling: A Distinct Role for the jasmonic acid precursor cis-(+)-12-oxo-phytodienoic acid (cis-OPDA)","volume":"3","author":"Dave","year":"2012","journal-title":"Front. Plant Sci."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"1638","DOI":"10.1093\/plphys\/kiab025","article-title":"Lipoxygenase functions in 1O2 production during root responses to osmotic stress","volume":"185","author":"Chen","year":"2021","journal-title":"Plant Physiol."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"832785","DOI":"10.3389\/fpls.2022.832785","article-title":"Identification of the LOX gene family in peanut and functional characterization of ahlox29 in drought tolerance","volume":"13","author":"Mou","year":"2022","journal-title":"Front. Plant Sci."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"4532","DOI":"10.1021\/jf9900682","article-title":"Effect of pepper lipoxygenase activity and its linked reactions on pigments of the pepper fruit","volume":"47","year":"1999","journal-title":"J. Agric. Food Chem."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"2875","DOI":"10.1021\/jf9000378","article-title":"Lipoxygenase gene expression in ripening kiwifruit in relation to ethylene and aroma production","volume":"57","author":"Zhang","year":"2009","journal-title":"J. Agric. Food Chem."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/23\/23\/15211\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T01:33:06Z","timestamp":1760146386000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/23\/23\/15211"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,12,2]]},"references-count":70,"journal-issue":{"issue":"23","published-online":{"date-parts":[[2022,12]]}},"alternative-id":["ijms232315211"],"URL":"https:\/\/doi.org\/10.3390\/ijms232315211","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,12,2]]}}}