{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T23:15:12Z","timestamp":1791501312214,"version":"4.3.4"},"reference-count":37,"publisher":"MDPI AG","issue":"23","license":[{"start":{"date-parts":[[2022,12,5]],"date-time":"2022-12-05T00:00:00Z","timestamp":1670198400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004699","name":"Chung Shan Medical University Hospital","doi-asserted-by":"publisher","award":["CSH-2022-C-009"],"award-info":[{"award-number":["CSH-2022-C-009"]}],"id":[{"id":"10.13039\/501100004699","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004699","name":"Chung Shan Medical University Hospital","doi-asserted-by":"publisher","award":["MOST 109-2320-B-040-024"],"award-info":[{"award-number":["MOST 109-2320-B-040-024"]}],"id":[{"id":"10.13039\/501100004699","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Ministry of Science and Technology Grant","award":["CSH-2022-C-009"],"award-info":[{"award-number":["CSH-2022-C-009"]}]},{"name":"Ministry of Science and Technology Grant","award":["MOST 109-2320-B-040-024"],"award-info":[{"award-number":["MOST 109-2320-B-040-024"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Diabetic nephropathy (DN) exacerbates renal tissue damage and is a major cause of end-stage renal disease. Reactive oxygen species play a vital role in hyperglycemia-induced renal injury. This study examined whether the oral hypoglycemic drug acarbose (Ab) could attenuate the progression of DN in type 2 diabetes mellitus mice. In this study, 50 mg\/kg body weight of Ab was administered to high-fat diet (HFD)-fed db\/db mice. Their body weight was recorded every week, and the serum glucose concentration was monitored every 2 weeks. Following their euthanasia, the kidneys of mice were analyzed through hematoxylin and eosin, periodic acid Schiff, Masson\u2019s trichrome, and immunohistochemistry (IHC) staining. The results revealed that Ab stabilized the plasma glucose and indirectly improved the insulin sensitivity and renal functional biomarkers in diabetic mice. In addition, diabetes-induced glomerular hypertrophy, the saccharide accumulation, and formation of collagen fiber were reduced in diabetic mice receiving Ab. Although the dosages of Ab cannot decrease the blood sugar in db\/db mice, our results indicate that Ab alleviates glucolipotoxicity-induced DN by inhibiting kidney fibrosis-related proteins through the Ras\/ERK pathway.<\/jats:p>","DOI":"10.3390\/ijms232315312","type":"journal-article","created":{"date-parts":[[2022,12,5]],"date-time":"2022-12-05T02:13:12Z","timestamp":1670206392000},"page":"15312","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Acarbose Protects Glucolipotoxicity-Induced Diabetic Nephropathy by Inhibiting Ras Expression in High-Fat Diet-Fed db\/db Mice"],"prefix":"10.3390","volume":"23","author":[{"given":"Tung-Wei","family":"Hung","sequence":"first","affiliation":[{"name":"School of Medicine, Institute of Medicine, Chung Shan Medical University, Taichung 402, Taiwan"},{"name":"Department of Medicine, Division of Nephrology, Chung Shan Medical University Hospital, Taichung 402, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meng-Hsun","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Nutrition, Chung Shan Medical University, No. 110, Sec. 1, Jianguo N. Road, Taichung 402, Taiwan"},{"name":"Department of Health Industry Technology Management, Chung Shan Medical University, Taichung 402, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7690-153X","authenticated-orcid":false,"given":"Tsung-Yuan","family":"Yang","sequence":"additional","affiliation":[{"name":"School of Medicine, Institute of Medicine, Chung Shan Medical University, Taichung 402, Taiwan"},{"name":"Department of Internal Medicine, Chung Shan Medical University Hospital, Taichung 402, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mon-Yuan","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Health Industry Technology Management, Chung Shan Medical University, Taichung 402, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jia-Yu","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Health Industry Technology Management, Chung Shan Medical University, Taichung 402, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kuei-Chuan","family":"Chan","sequence":"additional","affiliation":[{"name":"School of Medicine, Institute of Medicine, Chung Shan Medical University, Taichung 402, Taiwan"},{"name":"Department of Internal Medicine, Chung Shan Medical University Hospital, Taichung 402, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chau-Jong","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Health Industry Technology Management, Chung Shan Medical University, Taichung 402, Taiwan"},{"name":"Department of Medical Research, Chung Shan Medical University Hospital, Taichung 402, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,12,5]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1159\/000101797","article-title":"Cellular and molecular mechanisms of proteinuria in diabetic nephropathy","volume":"106","author":"Wolf","year":"2007","journal-title":"Nephron Physiol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"444","DOI":"10.1038\/ncpendmet0894","article-title":"The pathogenesis of diabetic nephropathy","volume":"4","author":"Dronavalli","year":"2008","journal-title":"Nat. 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