{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,13]],"date-time":"2025-11-13T07:23:08Z","timestamp":1763018588223,"version":"build-2065373602"},"reference-count":48,"publisher":"MDPI AG","issue":"23","license":[{"start":{"date-parts":[[2022,12,6]],"date-time":"2022-12-06T00:00:00Z","timestamp":1670284800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Veneto Institute of Oncology IOV-IRCCS","award":["5x1000"],"award-info":[{"award-number":["5x1000"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Merkel cell carcinoma (MCC) is a rare and aggressive cutaneous malignant tumor with neuroendocrine differentiation, with a rapidly growing incidence rate, high risk of recurrence, and aggressive behavior. The available therapeutic options for advanced disease are limited and there is a pressing need for new treatments. Tumors harboring fusions involving one of the neurotrophin receptor tyrosine kinase (NTRK) genes are now actionable with targeted inhibitors. NTRK-fused genes have been identified in neuroendocrine tumors of other sites; thus, a series of 76 MCCs were firstly analyzed with pan-TRK immunohistochemistry and the positive ones with real-time RT-PCR, RNA-based NGS, and FISH to detect the eventual underlying gene fusion. Despite 34 MCCs showing pan-TRK expression, NTRK fusions were not found in any cases. As in other tumors with neural differentiation, TRK expression seems to be physiological and not caused by gene fusions.<\/jats:p>","DOI":"10.3390\/ijms232315366","type":"journal-article","created":{"date-parts":[[2022,12,6]],"date-time":"2022-12-06T01:43:48Z","timestamp":1670291028000},"page":"15366","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["TRK Protein Expression in Merkel Cell Carcinoma Is Not Caused by NTRK Fusions"],"prefix":"10.3390","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0480-3412","authenticated-orcid":false,"given":"Rocco","family":"Cappellesso","sequence":"first","affiliation":[{"name":"Pathological Anatomy Unit, Padua University Hospital, 35121 Padua, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8126-9674","authenticated-orcid":false,"given":"Lorenzo","family":"Nicol\u00e8","sequence":"additional","affiliation":[{"name":"Department of Pathology, Angelo Hospital, 30174 Venice, Italy"},{"name":"Department of Medicine (DIMED), University of Padua, 35121 Padua, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2862-8014","authenticated-orcid":false,"given":"Paolo","family":"Del Fiore","sequence":"additional","affiliation":[{"name":"Soft-Tissue, Peritoneum and Melanoma Surgical Oncology Unit, Veneto Institute of Oncology IOV-IRCCS, 35128 Padua, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0720-8456","authenticated-orcid":false,"given":"Luisa","family":"Barzon","sequence":"additional","affiliation":[{"name":"Department of Molecular Medicine (DMM), University of Padua, 35121 Padua, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alessandro","family":"Sinigaglia","sequence":"additional","affiliation":[{"name":"Department of Molecular Medicine (DMM), University of Padua, 35121 Padua, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Silvia","family":"Riccetti","sequence":"additional","affiliation":[{"name":"Department of Molecular Medicine (DMM), University of Padua, 35121 Padua, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8340-3184","authenticated-orcid":false,"given":"Renato","family":"Franco","sequence":"additional","affiliation":[{"name":"Pathology Unit, University of Campania \u201cL. Vanvitelli\u201d, 80129 Naples, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Federica","family":"Zito Marino","sequence":"additional","affiliation":[{"name":"Pathology Unit, University of Campania \u201cL. Vanvitelli\u201d, 80129 Naples, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giada","family":"Munari","sequence":"additional","affiliation":[{"name":"Veneto Institute of Oncology IOV-IRCCS, 35128 Padua, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carolina","family":"Zamuner","sequence":"additional","affiliation":[{"name":"Veneto Institute of Oncology IOV-IRCCS, 35128 Padua, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0831-3971","authenticated-orcid":false,"given":"Francesco","family":"Cavallin","sequence":"additional","affiliation":[{"name":"Independent Statistician, 36020 Solagna, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marta","family":"Sbaraglia","sequence":"additional","affiliation":[{"name":"Department of Medicine (DIMED), University of Padua, 35121 Padua, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3245-2322","authenticated-orcid":false,"given":"Francesca","family":"Galuppini","sequence":"additional","affiliation":[{"name":"Department of Medicine (DIMED), University of Padua, 35121 Padua, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Franco","family":"Bassetto","sequence":"additional","affiliation":[{"name":"Department of Neurosciences (DNS), University of Padua, 35128 Padua, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mauro","family":"Alaibac","sequence":"additional","affiliation":[{"name":"Department of Medicine (DIMED), University of Padua, 35121 Padua, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9191-9124","authenticated-orcid":false,"given":"Vanna","family":"Chiarion-Sileni","sequence":"additional","affiliation":[{"name":"Melanoma Unit, Oncology 2 Unit, Veneto Institute of Oncology IOV-IRCCS, 35128 Padua, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luisa","family":"Piccin","sequence":"additional","affiliation":[{"name":"Melanoma Unit, Oncology 2 Unit, Veneto Institute of Oncology IOV-IRCCS, 35128 Padua, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9077-2471","authenticated-orcid":false,"given":"Clara","family":"Benna","sequence":"additional","affiliation":[{"name":"Department of Surgery, Oncology and Gastroenterology (DISCOG), University of Padua, 35128 Padua, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6515-5482","authenticated-orcid":false,"given":"Matteo","family":"Fassan","sequence":"additional","affiliation":[{"name":"Department of Medicine (DIMED), University of Padua, 35121 Padua, Italy"},{"name":"Veneto Institute of Oncology IOV-IRCCS, 35128 Padua, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Simone","family":"Mocellin","sequence":"additional","affiliation":[{"name":"Soft-Tissue, Peritoneum and Melanoma Surgical Oncology Unit, Veneto Institute of Oncology IOV-IRCCS, 35128 Padua, Italy"},{"name":"Department of Surgery, Oncology and Gastroenterology (DISCOG), University of Padua, 35128 Padua, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Angelo Paolo","family":"Dei Tos","sequence":"additional","affiliation":[{"name":"Department of Medicine (DIMED), University of Padua, 35121 Padua, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,12,6]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1038\/s41379-019-0394-6","article-title":"Danger is only skin deep: Aggressive epidermal carcinomas. 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