{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T23:36:54Z","timestamp":1790811414287,"version":"4.1.0"},"reference-count":44,"publisher":"MDPI AG","issue":"23","license":[{"start":{"date-parts":[[2022,12,6]],"date-time":"2022-12-06T00:00:00Z","timestamp":1670284800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002915","name":"Fondation pour la Recherche M\u00e9dicale","doi-asserted-by":"publisher","award":["EQU202003010171"],"award-info":[{"award-number":["EQU202003010171"]}],"id":[{"id":"10.13039\/501100002915","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Association Fran\u00e7ois Aupetit","award":["EQU202003010171"],"award-info":[{"award-number":["EQU202003010171"]}]},{"name":"Centre Hospitalo-Universitaire de Poitiers and Agence R\u00e9gionale de Sant\u00e9 Nouvelle-Aquitaine","award":["EQU202003010171"],"award-info":[{"award-number":["EQU202003010171"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Intestinal dysbiosis is a key feature in the pathogenesis of inflammatory bowel disease (IBD). Acyl-homoserine lactones (AHL) are bacterial quorum-sensing metabolites that may play a role in the changes in host cells-gut microbiota interaction observed during IBD. The objective of our study was to investigate the presence and expression of AHL synthases and receptor genes in the human gut ecosystem during IBD. We used an in silico approach, applied to the Inflammatory Bowel Disease Multi\u2019omics Database comprising bacterial metagenomic and metatranscriptomic data from stools of patients with Crohn\u2019s disease (CD) (n = 50), ulcerative colitis (UC) (n = 27) and non-IBD controls (n = 26). No known putative AHL synthase gene was identified; however, several putative luxR receptors were observed. Regarding the expression of these receptor genes, the luxR gene from Bacteroides dorei was under-expressed in IBD patients (p = 0.02) compared to non-IBD patients, especially in CD patients (p = 0.02). In the dysbiosis situation, one luxR receptor gene from Bacteroides fragilis appeared to be over-expressed (p = 0.04) compared to that of non-dysbiotic patients. Targeting LuxR receptors of bacterial quorum sensing might represent a new approach to modulate the gut microbiota in IBD.<\/jats:p>","DOI":"10.3390\/ijms232315404","type":"journal-article","created":{"date-parts":[[2022,12,7]],"date-time":"2022-12-07T01:47:46Z","timestamp":1670377666000},"page":"15404","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Impact of IBD-Associated Dysbiosis on Bacterial Quorum Sensing Mediated by Acyl-Homoserine Lactone in Human Gut Microbiota"],"prefix":"10.3390","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0452-3218","authenticated-orcid":false,"given":"Nathan","family":"Grellier","sequence":"first","affiliation":[{"name":"Centre de Recherche Saint-Antoine, Assistance Publique-H\u00f4pitaux de Paris, H\u00f4pital Saint Antoine, Service de Gastroent\u00e9rologie, Inserm, Sorbonne Universit\u00e9, F-75012 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3868-6362","authenticated-orcid":false,"given":"Marcelino T.","family":"Suzuki","sequence":"additional","affiliation":[{"name":"Laboratoire de Biodiversit\u00e9 et Biotechnologies Microbiennes, CNRS, Sorbonne Universit\u00e9, UAR3579, F-66650 Banyuls-sur-Mer, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Loic","family":"Brot","sequence":"additional","affiliation":[{"name":"Centre de Recherche Saint-Antoine, Assistance Publique-H\u00f4pitaux de Paris, H\u00f4pital Saint Antoine, Service de Gastroent\u00e9rologie, Inserm, Sorbonne Universit\u00e9, F-75012 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alice M. S.","family":"Rodrigues","sequence":"additional","affiliation":[{"name":"Observatoire Oc\u00e9anologique de Banyuls-sur-Mer, CNRS, Sorbonne Universit\u00e9, FR3724, F-66650 Banyuls-sur-Mer, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lydie","family":"Humbert","sequence":"additional","affiliation":[{"name":"Centre de Recherche Saint-Antoine, Assistance Publique-H\u00f4pitaux de Paris, H\u00f4pital Saint Antoine, Service de Gastroent\u00e9rologie, Inserm, Sorbonne Universit\u00e9, F-75012 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7810-6751","authenticated-orcid":false,"given":"Karine","family":"Escoubeyrou","sequence":"additional","affiliation":[{"name":"Observatoire Oc\u00e9anologique de Banyuls-sur-Mer, CNRS, Sorbonne Universit\u00e9, FR3724, F-66650 Banyuls-sur-Mer, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dominique","family":"Rainteau","sequence":"additional","affiliation":[{"name":"Centre de Recherche Saint-Antoine, Assistance Publique-H\u00f4pitaux de Paris, H\u00f4pital Saint Antoine, Service de Gastroent\u00e9rologie, Inserm, Sorbonne Universit\u00e9, F-75012 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Pierre","family":"Grill","sequence":"additional","affiliation":[{"name":"Centre de Recherche Saint-Antoine, Assistance Publique-H\u00f4pitaux de Paris, H\u00f4pital Saint Antoine, Service de Gastroent\u00e9rologie, Inserm, Sorbonne Universit\u00e9, F-75012 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4196-4386","authenticated-orcid":false,"given":"Rapha\u00ebl","family":"Lami","sequence":"additional","affiliation":[{"name":"Laboratoire de Biodiversit\u00e9 et Biotechnologies Microbiennes, CNRS, Sorbonne Universit\u00e9, UAR3579, F-66650 Banyuls-sur-Mer, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3596-9893","authenticated-orcid":false,"given":"Philippe","family":"Seksik","sequence":"additional","affiliation":[{"name":"Centre de Recherche Saint-Antoine, Assistance Publique-H\u00f4pitaux de Paris, H\u00f4pital Saint Antoine, Service de Gastroent\u00e9rologie, Inserm, Sorbonne Universit\u00e9, F-75012 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,12,6]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1038\/nature11234","article-title":"Structure, function and diversity of the healthy human microbiome","volume":"486","author":"Huttenhower","year":"2012","journal-title":"Nature"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1038\/s41575-019-0258-z","article-title":"Gut microbiota-derived metabolites as key actors in inflammatory bowel disease","volume":"17","author":"Lavelle","year":"2020","journal-title":"Nat. Rev. Gastroenterol. Hepatol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1016\/j.tim.2007.12.008","article-title":"The role of microbiota in infectious disease","volume":"16","author":"Stecher","year":"2008","journal-title":"Trends Microbiol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1038\/nri3430","article-title":"Role of the gut microbiota in immunity and inflammatory disease","volume":"13","author":"Kamada","year":"2013","journal-title":"Nat. Rev. Immunol."},{"key":"ref_5","first-page":"407","article-title":"Smoking and inflammatory bowel disease in families","volume":"83","author":"Smith","year":"1988","journal-title":"Am. J. Gastroenterol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1136\/gut.52.2.237","article-title":"Alterations of the dominant faecal bacterial groups in patients with Crohns disease of the colon","volume":"52","author":"Seksik","year":"2003","journal-title":"Gut"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1183","DOI":"10.1002\/ibd.20903","article-title":"Low counts of Faecalibacterium prausnitzii in colitis microbiota","volume":"15","author":"Sokol","year":"2009","journal-title":"Inflamm. Bowel Dis."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"573","DOI":"10.1038\/nrgastro.2017.88","article-title":"Gut microbiota and IBD: Causation or correlation?","volume":"14","author":"Ni","year":"2017","journal-title":"Nat. Rev. Gastroenterol. Hepatol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"598","DOI":"10.1038\/nm.4102","article-title":"CARD9 impacts colitis by altering gut microbiota metabolism of tryptophan into aryl hydrocarbon receptor ligands","volume":"22","author":"Lamas","year":"2016","journal-title":"Nat. Med."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"716","DOI":"10.1016\/j.chom.2018.05.003","article-title":"Gut Microbiota Regulation of Tryptophan Metabolism in Health and Disease","volume":"23","author":"Agus","year":"2018","journal-title":"Cell Host Microbe"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"531","DOI":"10.1136\/gutjnl-2012-302578","article-title":"Connecting dysbiosis, bile-acid dysmetabolism and gut inflammation in inflammatory bowel diseases","volume":"62","author":"Duboc","year":"2013","journal-title":"Gut"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1316","DOI":"10.1038\/s41385-019-0205-x","article-title":"Colitis susceptibility in mice with reactive oxygen species deficiency is mediated by mucus barrier and immune defense defects","volume":"12","author":"Aviello","year":"2019","journal-title":"Mucosal Immunol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"371","DOI":"10.1038\/s41579-019-0186-5","article-title":"Bacterial quorum sensing in complex and dynamically changing environments","volume":"17","author":"Mukherjee","year":"2019","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"4154","DOI":"10.1073\/pnas.81.13.4154","article-title":"Identification of genes and gene products necessary for bacterial bioluminescence","volume":"81","author":"Engebrecht","year":"1984","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"3000","DOI":"10.1128\/jb.173.9.3000-3009.1991","article-title":"Cloning and characterization of the Pseudomonas aeruginosa lasR gene, a transcriptional activator of elastase expression","volume":"173","author":"Gambello","year":"1991","journal-title":"J. Bacteriol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1097\/MOG.0b013e3280118289","article-title":"Quorum sensing by enteric pathogens","volume":"23","author":"Kendall","year":"2007","journal-title":"Curr. Opin. Gastroenterol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1128\/JB.01341-12","article-title":"Are there acyl-homoserine lactones within mammalian intestines?","volume":"195","author":"Swearingen","year":"2013","journal-title":"J. Bacteriol."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Dyszel, J.L., Soares, J.A., Swearingen, M.C., Lindsay, A., Smith, J.N., and Ahmer, B.M.M. (2010). E. coli K-12 and EHEC genes regulated by SdiA. PLoS ONE, 5.","DOI":"10.1371\/journal.pone.0008946"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"5733","DOI":"10.1128\/JB.183.19.5733-5742.2001","article-title":"SdiA of Salmonella enterica is a LuxR homolog that detects mixed microbial communities","volume":"183","author":"Michael","year":"2001","journal-title":"J. Bacteriol."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Landman, C., Grill, J.-P., Mallet, J.-M., Marteau, P., Humbert, L., Le Balc\u2019H, E., Maubert, M.-A., Perez, K., Chaara, W., and Brot, L. (2018). Inter-kingdom effect on epithelial cells of the N-Acyl homoserine lactone 3-oxo-C12:2, a major quorum-sensing molecule from gut microbiota. PLoS ONE, 13.","DOI":"10.1371\/journal.pone.0202587"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1832877","DOI":"10.1080\/21688370.2020.1832877","article-title":"The intestinal quorum sensing 3-oxo-C12:2 Acyl homoserine lactone limits cytokine-induced tight junction disruption","volume":"8","author":"Aguanno","year":"2020","journal-title":"Tissue Barriers"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"9440","DOI":"10.1038\/s41598-022-13451-3","article-title":"3-oxo-C12:2-HSL, quorum sensing molecule from human intestinal microbiota, inhibits pro-inflammatory pathways in immune cells via bitter taste receptors","volume":"12","author":"Coquant","year":"2022","journal-title":"Sci. Rep."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Peyrottes, A., Coquant, G., Brot, L., Rainteau, D., Seksik, P., Grill, J.-P., and Mallet, J.-M. (2020). Anti-Inflammatory Effects of Analogues of N-Acyl Homoserine Lactones on Eukaryotic Cells. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21249448"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1002\/jms.1275","article-title":"Profiling of N-acyl-homoserine lactones by liquid chromatography coupled with electrospray ionization and a hybrid quadrupole linear ion-trap and Fourier-transform ion-cyclotron-resonance mass spec-trometry (LC-ESI-LTQ-FTICR-MS)","volume":"43","author":"Cataldi","year":"2008","journal-title":"J. Mass. Spectrom."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"IBDMDB Investigators, Lloyd-Price, J., Arze, C., Ananthakrishnan, A.N., Schirmer, M., Avila-Pacheco, J., Poon, T.W., Andrews, E., Ajami, N.J., and Bonham, K.S. (2019). Multi-omics of the gut microbial ecosystem in inflammatory bowel diseases. Nature, 569, 655\u2013662.","DOI":"10.1038\/s41586-019-1237-9"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"514","DOI":"10.1016\/S0140-6736(80)92767-1","article-title":"A simple index of Crohns-disease activity","volume":"1","author":"Harvey","year":"1980","journal-title":"Lancet"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1136\/gut.43.1.29","article-title":"A simple clinical colitis activity index","volume":"43","author":"Walmsley","year":"1998","journal-title":"Gut"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"576","DOI":"10.1038\/nrmicro.2016.89","article-title":"Quorum sensing signal\u2013response systems in Gram-negative bacteria","volume":"14","author":"Papenfort","year":"2016","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"107","DOI":"10.3354\/ame01734","article-title":"Diversity of quorum sensing autoinducer synthases in the Global Ocean Sampling metagenomic database","volume":"74","author":"Doberva","year":"2015","journal-title":"Aquat. Microb. Ecol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1111\/j.1469-0691.2007.01925.x","article-title":"Quorum-sensing activity and related virulence factor expression in clinically pathogenic isolates of Pseudomonas aeruginosa","volume":"14","author":"Nguyen","year":"2008","journal-title":"Clin. Microbiol. Infect."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"7155","DOI":"10.1128\/jb.177.24.7155-7163.1995","article-title":"Synthesis of multiple exoproducts in Pseudomonas aeruginosa is under the control of RhlR-RhlI, another set of regulators in strain PAO1 with homology to the autoinducer-responsive LuxR-LuxI family","volume":"177","author":"Brint","year":"1995","journal-title":"J. Bacteriol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"e01417-15","DOI":"10.1128\/genomeA.01417-15","article-title":"Complete Genome Sequence of Serratia marcescens SmUNAM836, a Nonpigmented Multidrug-Resistant Strain Isolated from a Mexican Patient with Obstructive Pulmonary Disease","volume":"4","author":"Vinuesa","year":"2016","journal-title":"Genome Announc."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"525","DOI":"10.1128\/JB.01144-09","article-title":"The Citrobacter rodentium genome sequence reveals convergent evolution with human pathogenic Escherichia coli","volume":"192","author":"Petty","year":"2010","journal-title":"J. Bacteriol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"412","DOI":"10.1007\/s00248-007-9358-3","article-title":"Presence of quorum-sensing systems associated with multidrug resistance and biofilm formation in Bacteroides fragilis","volume":"56","author":"Pumbwe","year":"2008","journal-title":"Microb. Ecol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1016\/S0022-2836(05)80360-2","article-title":"Basic local alignment search tool","volume":"215","author":"Altschul","year":"1990","journal-title":"J. Mol. Biol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"R60","DOI":"10.1186\/gb-2011-12-6-r60","article-title":"Metagenomic biomarker discovery and explanation","volume":"12","author":"Segata","year":"2011","journal-title":"Genome Biol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"7247","DOI":"10.3748\/wjg.v27.i42.7247","article-title":"Gossip in the gut: Quorum sensing, a new player in the host-microbiota interactions","volume":"27","author":"Coquant","year":"2021","journal-title":"World J. Gastroenterol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1861","DOI":"10.1016\/j.celrep.2015.02.049","article-title":"Manipulation of the quorum sensing signal AI-2 affects the antibiotic-treated gut microbiota","volume":"10","author":"Thompson","year":"2015","journal-title":"Cell Rep."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1039","DOI":"10.1136\/gutjnl-2015-310746","article-title":"Fungal microbiota dysbiosis in IBD","volume":"66","author":"Sokol","year":"2017","journal-title":"Gut"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1425","DOI":"10.1023\/B:DDAS.0000042241.13489.88","article-title":"Are Helicobacter species and enterotoxigenic Bacteroides fragilis involved in inflammatory bowel disease?","volume":"49","author":"Basset","year":"2004","journal-title":"Dig. Dis. Sci."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1552","DOI":"10.1053\/j.gastro.2021.08.003","article-title":"Enterotoxigenic Bacteroides fragilis Promotes Intestinal Inflammation and Malignancy by Inhibiting Exosome-Packaged miR-149-3p","volume":"161","author":"Cao","year":"2021","journal-title":"Gastroenterology"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"852","DOI":"10.1038\/s41587-019-0209-9","article-title":"Reproducible, interactive, scalable and extensible microbiome data science using QIIME 2","volume":"37","author":"Bolyen","year":"2019","journal-title":"Nat. Biotechnol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"281","DOI":"10.3389\/fcimb.2018.00281","article-title":"Alterations in the Abundance and Co-occurrence of Akkermansia muciniphila and Faecalibacterium prausnitzii in the Colonic Mucosa of Inflammatory Bowel Disease Subjects","volume":"8","author":"Aldeguer","year":"2018","journal-title":"Front. Cell Infect. Microbiol."},{"key":"ref_44","first-page":"9","article-title":"PAST: Paleontological statistics software package for education and data analysis","volume":"4","author":"Hammer","year":"2001","journal-title":"Palaeontol. Electron."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/23\/23\/15404\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T01:34:59Z","timestamp":1760146499000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/23\/23\/15404"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,12,6]]},"references-count":44,"journal-issue":{"issue":"23","published-online":{"date-parts":[[2022,12]]}},"alternative-id":["ijms232315404"],"URL":"https:\/\/doi.org\/10.3390\/ijms232315404","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,12,6]]}}}