{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T00:51:45Z","timestamp":1791247905028,"version":"4.1.0"},"reference-count":39,"publisher":"MDPI AG","issue":"24","license":[{"start":{"date-parts":[[2022,12,7]],"date-time":"2022-12-07T00:00:00Z","timestamp":1670371200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Swedish Research Council","award":["2016-10279"],"award-info":[{"award-number":["2016-10279"]}]},{"name":"Swedish Research Council","award":["2019-01355"],"award-info":[{"award-number":["2019-01355"]}]},{"name":"Swedish Research Council","award":["CAN2017\/362"],"award-info":[{"award-number":["CAN2017\/362"]}]},{"name":"Swedish Research Council","award":["20 0795 PfF"],"award-info":[{"award-number":["20 0795 PfF"]}]},{"name":"Swedish Research Council","award":["N2018-0030"],"award-info":[{"award-number":["N2018-0030"]}]},{"name":"Swedish Research Council","award":["P2019-0001"],"award-info":[{"award-number":["P2019-0001"]}]},{"name":"Swedish Cancer Society","award":["2016-10279"],"award-info":[{"award-number":["2016-10279"]}]},{"name":"Swedish Cancer Society","award":["2019-01355"],"award-info":[{"award-number":["2019-01355"]}]},{"name":"Swedish Cancer Society","award":["CAN2017\/362"],"award-info":[{"award-number":["CAN2017\/362"]}]},{"name":"Swedish Cancer Society","award":["20 0795 PfF"],"award-info":[{"award-number":["20 0795 PfF"]}]},{"name":"Swedish Cancer Society","award":["N2018-0030"],"award-info":[{"award-number":["N2018-0030"]}]},{"name":"Swedish Cancer Society","award":["P2019-0001"],"award-info":[{"award-number":["P2019-0001"]}]},{"name":"Sven och Lilly Lawskis fond f\u00f6r naturvetenskaplig forskning","award":["2016-10279"],"award-info":[{"award-number":["2016-10279"]}]},{"name":"Sven och Lilly Lawskis fond f\u00f6r naturvetenskaplig forskning","award":["2019-01355"],"award-info":[{"award-number":["2019-01355"]}]},{"name":"Sven och Lilly Lawskis fond f\u00f6r naturvetenskaplig forskning","award":["CAN2017\/362"],"award-info":[{"award-number":["CAN2017\/362"]}]},{"name":"Sven och Lilly Lawskis fond f\u00f6r naturvetenskaplig forskning","award":["20 0795 PfF"],"award-info":[{"award-number":["20 0795 PfF"]}]},{"name":"Sven och Lilly Lawskis fond f\u00f6r naturvetenskaplig forskning","award":["N2018-0030"],"award-info":[{"award-number":["N2018-0030"]}]},{"name":"Sven och Lilly Lawskis fond f\u00f6r naturvetenskaplig forskning","award":["P2019-0001"],"award-info":[{"award-number":["P2019-0001"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Helicobacter pylori infection triggers inflammation that may lead to gastritis, stomach ulcers and cancer. Probiotic bacteria, such as Lactobacillus, have been of interest as treatment options, however, little is known about the molecular mechanisms of Lactobacillus-mediated inhibition of H. pylori pathogenesis. In this work, we investigated the effect of Lactobacillus culture supernatants, so-called conditioned medium (CM), from two gastric isolates, L. gasseri and L. oris, on the expression of transcriptional regulators in H. pylori. Among the four known two-component systems (TCSs), i.e., ArsRS, FlgRS, CheAY and CrdRS, the flagellar regulator gene flgR and the acid resistance associated arsS gene were down-regulated by L. gasseri CM, whereas expression of the other TCS-genes remained unaffected. L. gasseri CM also reduced the motility of H. pylori, which is in line with reduced flgR expression. Furthermore, among six transcription factors of H. pylori only the ferric uptake regulator gene fur was regulated by L. gasseri CM. Deletion of fur further led to dramatically increased sensitivity to the antimicrobial peptide LL-37. Taken together, the results highlight that released\/secreted factors of some lactobacilli, but not all, downregulate transcriptional regulators involved in motility, acid tolerance and LL-37 sensitivity of H. pylori.<\/jats:p>","DOI":"10.3390\/ijms232415451","type":"journal-article","created":{"date-parts":[[2022,12,7]],"date-time":"2022-12-07T01:47:46Z","timestamp":1670377666000},"page":"15451","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Lactobacilli Downregulate Transcription Factors in Helicobacter pylori That Affect Motility, Acid Tolerance and Antimicrobial Peptide Survival"],"prefix":"10.3390","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4545-1265","authenticated-orcid":false,"given":"Fanglei","family":"Zuo","sequence":"first","affiliation":[{"name":"Department of Molecular Biosciences, The Wenner-Gren Institute, Stockholm University, 10691 Stockholm, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tanvi","family":"Somiah","sequence":"additional","affiliation":[{"name":"Department of Molecular Biosciences, The Wenner-Gren Institute, Stockholm University, 10691 Stockholm, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanna G.","family":"Gebremariam","sequence":"additional","affiliation":[{"name":"Department of Molecular Biosciences, The Wenner-Gren Institute, Stockholm University, 10691 Stockholm, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ann-Beth","family":"Jonsson","sequence":"additional","affiliation":[{"name":"Department of Molecular Biosciences, The Wenner-Gren Institute, Stockholm University, 10691 Stockholm, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,12,7]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"629","DOI":"10.1038\/nrgastro.2010.154","article-title":"Mechanisms of disease: Helicobacter pylori virulence factors","volume":"7","author":"Yamaoka","year":"2010","journal-title":"Nat. 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