{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T16:41:33Z","timestamp":1790095293323,"version":"4.0.1"},"reference-count":50,"publisher":"MDPI AG","issue":"24","license":[{"start":{"date-parts":[[2022,12,7]],"date-time":"2022-12-07T00:00:00Z","timestamp":1670371200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Key Technologies R&amp;D Program for Modernization of Traditional Chinese Medicine","award":["2017YFC1701300"],"award-info":[{"award-number":["2017YFC1701300"]}]},{"name":"National Key Technologies R&amp;D Program for Modernization of Traditional Chinese Medicine","award":["2021-QYZL-02"],"award-info":[{"award-number":["2021-QYZL-02"]}]},{"name":"National Key Technologies R&amp;D Program for Modernization of Traditional Chinese Medicine","award":["2022ZDLSF05-01"],"award-info":[{"award-number":["2022ZDLSF05-01"]}]},{"name":"National Key Technologies R&amp;D Program for Modernization of Traditional Chinese Medicine","award":["GK202205006"],"award-info":[{"award-number":["GK202205006"]}]},{"name":"Administration of Traditional Chinese Medicine of Shaanxi","award":["2017YFC1701300"],"award-info":[{"award-number":["2017YFC1701300"]}]},{"name":"Administration of Traditional Chinese Medicine of Shaanxi","award":["2021-QYZL-02"],"award-info":[{"award-number":["2021-QYZL-02"]}]},{"name":"Administration of Traditional Chinese Medicine of Shaanxi","award":["2022ZDLSF05-01"],"award-info":[{"award-number":["2022ZDLSF05-01"]}]},{"name":"Administration of Traditional Chinese Medicine of Shaanxi","award":["GK202205006"],"award-info":[{"award-number":["GK202205006"]}]},{"name":"Key Industry Chain Project of Shaanxi province","award":["2017YFC1701300"],"award-info":[{"award-number":["2017YFC1701300"]}]},{"name":"Key Industry Chain Project of Shaanxi province","award":["2021-QYZL-02"],"award-info":[{"award-number":["2021-QYZL-02"]}]},{"name":"Key Industry Chain Project of Shaanxi province","award":["2022ZDLSF05-01"],"award-info":[{"award-number":["2022ZDLSF05-01"]}]},{"name":"Key Industry Chain Project of Shaanxi province","award":["GK202205006"],"award-info":[{"award-number":["GK202205006"]}]},{"name":"Fundamental Research Funds for the Central Universities","award":["2017YFC1701300"],"award-info":[{"award-number":["2017YFC1701300"]}]},{"name":"Fundamental Research Funds for the Central Universities","award":["2021-QYZL-02"],"award-info":[{"award-number":["2021-QYZL-02"]}]},{"name":"Fundamental Research Funds for the Central Universities","award":["2022ZDLSF05-01"],"award-info":[{"award-number":["2022ZDLSF05-01"]}]},{"name":"Fundamental Research Funds for the Central Universities","award":["GK202205006"],"award-info":[{"award-number":["GK202205006"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Scutellaria baicalensis Georgi is an annual herb from the Scutellaria genus that has been extensively used as a traditional medicine for over 2000 years in China. Baicalin and other flavonoids have been identified as the principal bioactive ingredients. The biosynthetic pathway of baicalin in S. baicalensis has been elucidated; however, the specific functions of R2R3-MYB TF, which regulates baicalin synthesis, has not been well characterized in S. baicalensis to date. Here, a S20 R2R3-MYB TF (SbMYB12), which encodes 263 amino acids with a length of 792 bp, was expressed in all tested tissues (mainly in leaves) and responded to exogenous hormone methyl jasmonate (MeJA) treatment. The overexpression of SbMYB12 significantly promoted the accumulation of flavonoids such as baicalin and wogonoside in S. baicalensis hairy roots. Furthermore, biochemical experiments revealed that SbMYB12 is a nuclear-localized transcription activator that binds to the SbCCL7-4, SbCHI-2, and SbF6H-1 promoters to activate their expression. These results illustrate that SbMYB12 positively regulates the generation of baicalin and wogonoside. In summary, this work revealed a novel S20 R2R3-MYB regulator and enhances our understanding of the transcriptional and regulatory mechanisms of baicalin biosynthesis, as well as sheds new light on metabolic engineering in S. baicalensis.<\/jats:p>","DOI":"10.3390\/ijms232415452","type":"journal-article","created":{"date-parts":[[2022,12,7]],"date-time":"2022-12-07T05:50:52Z","timestamp":1670392252000},"page":"15452","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":31,"title":["A Novel R2R3-MYB Transcription Factor SbMYB12 Positively Regulates Baicalin Biosynthesis in Scutellaria baicalensis Georgi"],"prefix":"10.3390","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8952-1088","authenticated-orcid":false,"given":"Wentao","family":"Wang","sequence":"first","affiliation":[{"name":"National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest China, Key Laboratory of the Ministry of Education for Medicinal Resources and Natural Pharmaceutical Chemistry, Shaanxi Normal University, Xi\u2019an 710062, China"},{"name":"University of Chinese Academy of Sciences, Beijing 100049, China"},{"name":"Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suying","family":"Hu","sequence":"additional","affiliation":[{"name":"National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest China, Key Laboratory of the Ministry of Education for Medicinal Resources and Natural Pharmaceutical Chemistry, Shaanxi Normal University, Xi\u2019an 710062, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Yang","sequence":"additional","affiliation":[{"name":"National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest China, Key Laboratory of the Ministry of Education for Medicinal Resources and Natural Pharmaceutical Chemistry, Shaanxi Normal University, Xi\u2019an 710062, China"},{"name":"University of Chinese Academy of Sciences, Beijing 100049, China"},{"name":"National Maize Improvement Center, College of Agronomy and Biotechnology, China Agricultural University, Beijing 100094, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caijuan","family":"Zhang","sequence":"additional","affiliation":[{"name":"National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest China, Key Laboratory of the Ministry of Education for Medicinal Resources and Natural Pharmaceutical Chemistry, Shaanxi Normal University, Xi\u2019an 710062, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tong","family":"Zhang","sequence":"additional","affiliation":[{"name":"National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest China, Key Laboratory of the Ministry of Education for Medicinal Resources and Natural Pharmaceutical Chemistry, Shaanxi Normal University, Xi\u2019an 710062, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3437-7386","authenticated-orcid":false,"given":"Donghao","family":"Wang","sequence":"additional","affiliation":[{"name":"National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest China, Key Laboratory of the Ministry of Education for Medicinal Resources and Natural Pharmaceutical Chemistry, Shaanxi Normal University, Xi\u2019an 710062, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3188-6663","authenticated-orcid":false,"given":"Xiaoyan","family":"Cao","sequence":"additional","affiliation":[{"name":"National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest China, Key Laboratory of the Ministry of Education for Medicinal Resources and Natural Pharmaceutical Chemistry, Shaanxi Normal University, Xi\u2019an 710062, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhezhi","family":"Wang","sequence":"additional","affiliation":[{"name":"National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest China, Key Laboratory of the Ministry of Education for Medicinal Resources and Natural Pharmaceutical Chemistry, Shaanxi Normal University, Xi\u2019an 710062, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,12,7]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Wang, Z., Tang, J., Hu, R., Wu, P., Hou, X.L., Song, X.M., and Xiong, A.S. 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