{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T07:06:44Z","timestamp":1785481604775,"version":"3.56.0"},"reference-count":66,"publisher":"MDPI AG","issue":"24","license":[{"start":{"date-parts":[[2022,12,8]],"date-time":"2022-12-08T00:00:00Z","timestamp":1670457600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Chinese National Science Foundation","award":["31972651"],"award-info":[{"award-number":["31972651"]}]},{"name":"Chinese National Science Foundation","award":["31101826"],"award-info":[{"award-number":["31101826"]}]},{"name":"Chinese National Science Foundation","award":["2021YFD1800404"],"award-info":[{"award-number":["2021YFD1800404"]}]},{"name":"Chinese National Science Foundation","award":["2017YFD0500203"],"award-info":[{"award-number":["2017YFD0500203"]}]},{"name":"National Key Research and Development Program of China","award":["31972651"],"award-info":[{"award-number":["31972651"]}]},{"name":"National Key Research and Development Program of China","award":["31101826"],"award-info":[{"award-number":["31101826"]}]},{"name":"National Key Research and Development Program of China","award":["2021YFD1800404"],"award-info":[{"award-number":["2021YFD1800404"]}]},{"name":"National Key Research and Development Program of China","award":["2017YFD0500203"],"award-info":[{"award-number":["2017YFD0500203"]}]},{"name":"the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)","award":["31972651"],"award-info":[{"award-number":["31972651"]}]},{"name":"the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)","award":["31101826"],"award-info":[{"award-number":["31101826"]}]},{"name":"the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)","award":["2021YFD1800404"],"award-info":[{"award-number":["2021YFD1800404"]}]},{"name":"the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)","award":["2017YFD0500203"],"award-info":[{"award-number":["2017YFD0500203"]}]},{"name":"Yangzhou University Interdisciplinary Research Foundation for Veterinary Medicine Discipline of Targeted Support","award":["31972651"],"award-info":[{"award-number":["31972651"]}]},{"name":"Yangzhou University Interdisciplinary Research Foundation for Veterinary Medicine Discipline of Targeted Support","award":["31101826"],"award-info":[{"award-number":["31101826"]}]},{"name":"Yangzhou University Interdisciplinary Research Foundation for Veterinary Medicine Discipline of Targeted Support","award":["2021YFD1800404"],"award-info":[{"award-number":["2021YFD1800404"]}]},{"name":"Yangzhou University Interdisciplinary Research Foundation for Veterinary Medicine Discipline of Targeted Support","award":["2017YFD0500203"],"award-info":[{"award-number":["2017YFD0500203"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Avian pathogenic Escherichia coli (APEC) is an important member of extraintestinal pathogenic Escherichia coli (ExPEC). It shares similar pathogenic strategies with neonatal meningitis E. coli (NMEC) and may threaten human health due to its potential zoonosis. RyhB is a small non-coding RNA that regulates iron homeostasis in E. coli. However, it is unclear whether RyhB regulates meningitis occurrence. To investigate the function of RyhB in the development of meningitis, we constructed the deletion mutant APEC XM\u2206ryhB and the complemented mutant APEC XM\u2206ryhB\/pryhB, established a mouse meningitis model and evaluated the role of RyhB in virulence of APEC. The results showed that the deletion of ryhB decreased biofilm formation, adhesion to the brain microvascular endothelial cell line bEnd.3 and serum resistance. RNA-seq data showed that the expression of multiple virulence-related genes changed in the ryhB deletion mutant in the presence of duck serum. Deletion of ryhB reduced the clinical symptoms of mice, such as opisthotonus, diarrhea and neurological signs, when challenged with APEC. Compared with the mice infected with the wild-type APEC, fewer histopathological lesions were observed in the brain of mice infected with the ryhB deletion mutant APEC XM\u2206ryhB. The bacterial loads in the tissues and the relative expression of cytokines (IL-1\u03b2, IL-6, and TNF-\u03b1) in the brain significantly decreased when challenged with the APEC XM\u2206ryhB. The expressions of tight junction proteins (claudin-5, occludin and ZO-1) were not reduced in the brain of mice infected with APEC XM\u2206ryhB; that is, the blood-brain barrier permeability of mice was not significantly damaged. In conclusion, RyhB contributes to the pathogenicity of APEC XM in the meningitis-causing process by promoting biofilm formation, adhesion to endothelial cells, serum resistance and virulence-related genes expression.<\/jats:p>","DOI":"10.3390\/ijms232415532","type":"journal-article","created":{"date-parts":[[2022,12,8]],"date-time":"2022-12-08T02:51:56Z","timestamp":1670467916000},"page":"15532","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["RyhB in Avian Pathogenic Escherichia coli Regulates the Expression of Virulence-Related Genes and Contributes to Meningitis Development in a Mouse Model"],"prefix":"10.3390","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6547-9417","authenticated-orcid":false,"given":"Xia","family":"Meng","sequence":"first","affiliation":[{"name":"Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Diseases and Zoonoses, College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China"},{"name":"International Research Laboratory of Prevention and Control of Important Animal Infectious Diseases and Zoonotic Diseases of Jiangsu Higher Education Institutions, Yangzhou University, Yangzhou 225009, China"},{"name":"Joint International Research Laboratory of Agriculture and Agri-Product Safety, The Ministry of Education of China, Yangzhou 225009, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanfei","family":"Chen","sequence":"additional","affiliation":[{"name":"Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Diseases and Zoonoses, College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China"},{"name":"International Research Laboratory of Prevention and Control of Important Animal Infectious Diseases and Zoonotic Diseases of Jiangsu Higher Education Institutions, Yangzhou University, Yangzhou 225009, China"},{"name":"Joint International Research Laboratory of Agriculture and Agri-Product Safety, The Ministry of Education of China, Yangzhou 225009, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peili","family":"Wang","sequence":"additional","affiliation":[{"name":"Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Diseases and Zoonoses, College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mengping","family":"He","sequence":"additional","affiliation":[{"name":"Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Diseases and Zoonoses, College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China"},{"name":"International Research Laboratory of Prevention and Control of Important Animal Infectious Diseases and Zoonotic Diseases of Jiangsu Higher Education Institutions, Yangzhou University, Yangzhou 225009, 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Co-Innovation Center for the Prevention and Control of Important Animal Infectious Diseases and Zoonoses, College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China"},{"name":"International Research Laboratory of Prevention and Control of Important Animal Infectious Diseases and Zoonotic Diseases of Jiangsu Higher Education Institutions, Yangzhou University, Yangzhou 225009, China"},{"name":"Joint International Research Laboratory of Agriculture and Agri-Product Safety, The Ministry of Education of China, Yangzhou 225009, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guoqiang","family":"Zhu","sequence":"additional","affiliation":[{"name":"Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Diseases and Zoonoses, College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China"},{"name":"International Research Laboratory of Prevention and Control of Important Animal Infectious Diseases and 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