{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T16:33:47Z","timestamp":1790699627934,"version":"4.1.0"},"reference-count":120,"publisher":"MDPI AG","issue":"24","license":[{"start":{"date-parts":[[2022,12,8]],"date-time":"2022-12-08T00:00:00Z","timestamp":1670457600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004442","name":"National Science Centre, Poland","doi-asserted-by":"publisher","award":["UMO-2020\/39\/B\/NZ9\/00491"],"award-info":[{"award-number":["UMO-2020\/39\/B\/NZ9\/00491"]}],"id":[{"id":"10.13039\/501100004442","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Fungal phytopathogens are challenging to control due to their penetration into plant tissues. Therefore, plant-colonizing bacteria could serve as an excellent weapon in fighting fungal infections. In this study, we aim to determine the biocontrol potential of the new endophytic strain Serratia quinivorans KP32, isolated from the roots of Petroselinum crispum L.; identify the related mechanisms; and understand the basis of its antagonistic interaction with taxonomically diverse fungi at the molecular level. The KP32 strain presented biological activity against Rhizoctonia solani, Colletotrichum dematium, Fusarium avenaceum, and Sclerotinia sclerotiorum, and its ability to inhibit the growth of the phytopathogens was found to be mediated by a broad spectrum of biocontrol features, such as the production of a number of lytic enzymes (amylases, chitinases, and proteases), siderophores, volatile organic and inorganic compounds, salicylic acid, and N-acyl-homoserine lactones. The higher expression of chitinase (chiA) and genes involved in the biosynthesis of hydrogen cyanide (hcnC), enterobactin (entB), and acetoin (budA) in bacteria exposed to fungal filtrates confirmed that these factors could act in combination, leading to a synergistic inhibitory effect of the strain against phytopathogens. We also confirm the active movement, self-aggregation, exopolysaccharide production, and biofilm formation abilities of the KP32 strain, which are essential for effective plant colonization. Its biological activity and colonization potential indicate that KP32 holds tremendous potential for use as an active biopesticide and plant growth promoter.<\/jats:p>","DOI":"10.3390\/ijms232415561","type":"journal-article","created":{"date-parts":[[2022,12,9]],"date-time":"2022-12-09T01:51:26Z","timestamp":1670550686000},"page":"15561","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Genetic Determinants of Antagonistic Interactions and the Response of New Endophytic Strain Serratia quinivorans KP32 to Fungal Phytopathogens"],"prefix":"10.3390","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7467-7418","authenticated-orcid":false,"given":"Daria","family":"Chlebek","sequence":"first","affiliation":[{"name":"Institute of Biology, Biotechnology and Environmental Protection, Faculty of Natural Sciences, University of Silesia in Katowice, Jagiello\u0144ska 28, 40-032 Katowice, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valeriia","family":"Grebtsova","sequence":"additional","affiliation":[{"name":"Institute of Biology, Biotechnology and Environmental Protection, Faculty of Natural Sciences, University of Silesia in Katowice, Jagiello\u0144ska 28, 40-032 Katowice, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2616-9539","authenticated-orcid":false,"given":"Artur","family":"Pi\u0144ski","sequence":"additional","affiliation":[{"name":"Institute of Biology, Biotechnology and Environmental Protection, Faculty of Natural Sciences, University of Silesia in Katowice, Jagiello\u0144ska 28, 40-032 Katowice, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2496-6200","authenticated-orcid":false,"given":"Joanna","family":"\u017bur-Pi\u0144ska","sequence":"additional","affiliation":[{"name":"Institute of Biology, Biotechnology and Environmental Protection, Faculty of Natural Sciences, University of Silesia in Katowice, Jagiello\u0144ska 28, 40-032 Katowice, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2821-568X","authenticated-orcid":false,"given":"Katarzyna","family":"Hupert-Kocurek","sequence":"additional","affiliation":[{"name":"Institute of Biology, Biotechnology and Environmental Protection, Faculty of Natural Sciences, University of Silesia in Katowice, Jagiello\u0144ska 28, 40-032 Katowice, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,12,8]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Marques-Pereira, C., Proen\u00e7a, D.N., and Morais, P.V. (2020). Genome sequences of Serratia strains revealed common genes in both serratomolides gene clusters. Biology, 9.","DOI":"10.3390\/biology9120482"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"3635","DOI":"10.1111\/1462-2920.13241","article-title":"Biosynthesis of the acetyl-CoA carboxylase-inhibiting antibiotic, andrimid in Serratia is regulated by Hfq and the LysR-type transcriptional regulator, AdmX","volume":"18","author":"Matilla","year":"2016","journal-title":"Environ. Microbiol."},{"key":"ref_3","first-page":"555814","article-title":"Serratia a novel source of secondary metabolites","volume":"11","year":"2018","journal-title":"Adv. Biotechnol. Microbiol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"126956","DOI":"10.1016\/j.micres.2021.126956","article-title":"The endophytic bacterial entomopathogen Serratia marcescens promotes plant growth and improves resistance against Nilaparvata lugens in rice","volume":"256","author":"Niu","year":"2022","journal-title":"Microbiol. Res."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Vaughan, A.L., Altermann, E., Glare, T.R., and Hurst, M.R. (2022). Genome sequence of the entomopathogenic Serratia entomophila isolate 626 and characterisation of the species specific itaconate degradation pathway. BMC Genom., 23.","DOI":"10.1186\/s12864-022-08938-2"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"577","DOI":"10.1007\/s11101-019-09657-5","article-title":"Biocontrol capabilities of the genus Serratia","volume":"19","author":"Soenens","year":"2020","journal-title":"Phytochem. Rev."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Lahlali, R., Ezrari, S., Radouane, N., Kenfaoui, J., Esmaeel, Q., El Hamss, H., Belabess, Z., and Barka, E.A. (2022). Biological control of plant pathogens: A global perspective. Microorganisms, 10.","DOI":"10.3390\/microorganisms10030596"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1111\/j.1439-0434.2010.01748.x","article-title":"Histological and ultrastructural studies on the curative effects of mandipropamid on Plasmopara viticola","volume":"159","author":"Toffolatti","year":"2010","journal-title":"J. Phytopathol."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Hazarika, D.J., Goswami, G., Gautom, T., Parveen, A., Das, P., Barooah, M., and Chandra Boro, R. (2019). Lipopeptide mediated biocontrol activity of endophytic Bacillus subtilis against fungal phytopathogens. BMC Microbiol., 19.","DOI":"10.1186\/s12866-019-1440-8"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"533","DOI":"10.1139\/cjm-2014-0212","article-title":"The role of the chi1 gene from the endophytic bacteria Serratia proteamaculans 336x in the biological control of wheat take-all","volume":"60","author":"Wang","year":"2014","journal-title":"Can. J. Microbiol."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Dhar Purkayastha, G., Mangar, P., Saha, A., and Saha, D. (2018). Evaluation of the biocontrol efficacy of a Serratia marcescens strain indigenous to tea rhizosphere for the management of root rot disease in tea. PLoS ONE, 13.","DOI":"10.1371\/journal.pone.0191761"},{"key":"ref_12","first-page":"65","article-title":"Management of Plant Diseases by PGPR-Mediated Induced Resistance with Special Reference to Tea and Rice Crops","volume":"Volume 13","author":"Sayyed","year":"2019","journal-title":"Plant Growth Promoting Rhizobacteria for Sustainable Stress Management. Microorganisms for Sustainability"},{"key":"ref_13","first-page":"169","article-title":"Role of Serratia sp. as Biocontrol Agent and Plant Growth Stimulator, with Prospects of Biotic Stress Management in Plant","volume":"Volume 13","author":"Sayyed","year":"2019","journal-title":"Plant Growth Promoting Rhizobacteria for Sustainable Stress Management. Microorganisms for Sustainability"},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Su, C., Xiang, Z., Liu, Y., Zhao, X., Sun, Y., Li, Z., Li, L., Chang, F., Chen, T., and Wen, X. (2016). Analysis of the genomic sequences and metabolites of Serratia surfactantfaciens sp. nov. YD25T that simultaneously produces prodigiosin and serrawettin W2. BMC Genom., 17.","DOI":"10.1186\/s12864-016-3171-7"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1465","DOI":"10.1007\/s11274-010-0321-y","article-title":"Biocontrol potential of an endophytic Serratia sp. G3 and its mode of action","volume":"26","author":"Liu","year":"2010","journal-title":"World J. Microbiol. Biotechnol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1016\/S0038-0717(02)00283-3","article-title":"Soil-borne strain IC14 of Serratia plymuthica with multiple mechanisms of antifungal activity provides biocontrol of Botrytis cinerea and Sclerotinia sclerotiorum diseases","volume":"35","author":"Kamensky","year":"2003","journal-title":"Soil Biol. Biochem."},{"key":"ref_17","first-page":"46","article-title":"Using Serratia plymuthica to control fungal pathogens of plant","volume":"2","year":"2007","journal-title":"CAB Rev."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1111\/1758-2229.12203","article-title":"Transcriptional responses of the bacterial antagonist Serratia plymuthica to the fungal phytopathogen Rhizoctonia solani","volume":"7","author":"Neupane","year":"2015","journal-title":"Environ. Microbiol. Rep."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1007\/s11274-011-0803-6","article-title":"Production of prodigiosin and chitinases by tropical Serratia marcescens strains with potential to control plant pathogens","volume":"28","author":"Dunn","year":"2012","journal-title":"World J. Microbiol. Biotechnol."},{"key":"ref_20","first-page":"417","article-title":"Antagonistic rhizobacteria and jasmonic acid induce resistance against tomato bacterial spot","volume":"74","author":"Ferraz","year":"2015","journal-title":"Plant Prot. Sci."},{"key":"ref_21","first-page":"1","article-title":"Entry, colonization, and distribution of endophytic microorganisms in plants","volume":"Volume 1","author":"Kumar","year":"2019","journal-title":"Microbial Endophytes: Functional Biology and Applications"},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Nelkner, J., Tejerizo, G.T., Hassa, J., Lin, T.W., Witte, J., Verwaaijen, B., Winkler, A., Bunk, B., Spr\u00f6er, C., and Overmann, J. (2019). Genetic potential of the biocontrol agent Pseudomonas brassicacearum (formerly P. trivialis) 3Re2-7 unraveled by genome sequencing and mining, comparative genomics and transcriptomics. Genes, 10.","DOI":"10.3390\/genes10080601"},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Li, J., Yang, Y., Dubern, J.F., Li, H., Halliday, N., Chernin, L., Gao, K., C\u00e1mara, M., and Liu, X. (2015). Regulation of GacA in Pseudomonas chlororaphis Strains Shows a Niche Specificity. PLoS ONE, 10.","DOI":"10.1371\/journal.pone.0137553"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"18006","DOI":"10.1038\/s41598-019-54601-4","article-title":"Serratia liquefaciens FG3 isolated from a metallophyte plant sheds light on the evolution and mechanisms of adaptive traits in extreme environments","volume":"9","author":"Caneschi","year":"2019","journal-title":"Sci. Rep."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Matteoli, F.P., Passarelli-Araujo, H., Reis, R.J.A., da Rocha, L.O., de Souza, E.M., Aravind, L., Olivares, F.L., and Venancio, T.M. (2018). Genome sequencing and assessment of plant growth-promoting properties of a Serratia marcescens strain isolated from vermicompost. BMC Genom., 16.","DOI":"10.1101\/288084"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1146\/annurev.micro.60.080805.142106","article-title":"Curli biogenesis and function","volume":"60","author":"Barnhart","year":"2006","journal-title":"Annu. Rev. Microbiol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"6645","DOI":"10.3390\/s120506645","article-title":"Detection of AI-2 receptors in genomes of Enterobacteriaceae suggests a role of type-2 quorum sensing in closed ecosystems","volume":"12","author":"Rezzonico","year":"2012","journal-title":"Sensors"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"369","DOI":"10.3389\/fmicb.2020.00369","article-title":"Genome Insights of the Plant-Growth Promoting Bacterium Cronobacter muytjensii JZ38 With Volatile-Mediated Antagonistic Activity Against Phytophthora infestans","volume":"11","author":"Eida","year":"2020","journal-title":"Front. Microbiol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"2023","DOI":"10.3389\/fmicb.2017.02023","article-title":"Complete genome sequence analysis of Enterobacter sp. SA187, a plant multi-stress tolerance promoting endophytic bacterium","volume":"8","author":"Lafi","year":"2017","journal-title":"Front. Microbiol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1128\/microbiolspec.VMBF-0012-2015","article-title":"Bacterial Secretion Systems: An Overview","volume":"4","author":"Green","year":"2016","journal-title":"Microbiol. Spectr."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"920","DOI":"10.1038\/s41564-018-0191-x","article-title":"The type VI secretion system deploys antifungal effectors against microbial competitors","volume":"3","author":"Trunk","year":"2018","journal-title":"Nat. Microbiol."},{"key":"ref_32","first-page":"371","article-title":"Wykrywanie biofilmu Pseudomonas aeruginosa na biomateria\u0142ach medycznych","volume":"55","author":"Wolska","year":"2003","journal-title":"Med. Do\u015bwiadczalna Mikrobiol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"891","DOI":"10.1111\/j.1600-0463.2007.apm_630.x","article-title":"Quantification of biofilm in microtiter plates: Overview of testing conditions and practical recommendations for assessment of biofilm production by staphylococci","volume":"115","author":"Stepanovic","year":"2007","journal-title":"APMIS"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"575578","DOI":"10.3389\/fmicb.2020.575578","article-title":"A crosstalk between Brachypodium root exudates, organic acids, and Bacillus velezensis B26, a growth promoting bacterium","volume":"11","author":"Sharma","year":"2020","journal-title":"Front. Microbiol."},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"He, D.-C., He, M.-H., Amalin, D.M., Liu, W., Alvindia, D.G., and Zhan, J. (2021). Biological Control of Plant Diseases: An Evolutionary and Eco-Economic Consideration. Pathogens, 10.","DOI":"10.3390\/pathogens10101311"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"6015","DOI":"10.1007\/s11033-020-05676-0","article-title":"Plant growth promoting Pseudomonas aeruginosa from Valeriana wallichii displays antagonistic potential against three phytopathogenic fungi","volume":"47","author":"Chandra","year":"2020","journal-title":"Mol. Biol. Rep."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1016\/j.micres.2019.02.001","article-title":"Plant beneficial endophytic bacteria: Mechanisms, diversity, host range and genetic determinants","volume":"221","author":"Afzal","year":"2019","journal-title":"Microbiol. Res."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"221","DOI":"10.5423\/PPJ.2002.18.4.221","article-title":"In vitro and in vivo activities of biocontrol agent, Serratia plymuthica A21-4, against Phytophthora capsici","volume":"18","author":"Shen","year":"2002","journal-title":"Plant Pathol. J."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1007\/s002030100347","article-title":"Purification and properties of two chitinolytic enzymes of Serratia plymuthica HRO-C48","volume":"176","author":"Frankowski","year":"2001","journal-title":"Arch. Microbiol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1016\/j.cropro.2004.07.004","article-title":"Biocontrol agents applied individually and in combination for suppression of soilborne diseases of cucumber","volume":"24","author":"Roberts","year":"2005","journal-title":"Crop Prot."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1007\/s12088-011-0065-x","article-title":"Isolation of Serratia marcescens SR1 as a source of chitinase having potentiality of using as a biocontrol agent","volume":"51","author":"Parani","year":"2011","journal-title":"Indian J. Microbiol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1080\/17429140903551738","article-title":"Influence of Serratia marcescens TRS-1 on growth promotion and induction of resistance in Camellia sinensis against Fomes lamaoensis","volume":"5","author":"Chakraborty","year":"2010","journal-title":"J. Plant Interact."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1007\/s40003-012-0019-5","article-title":"Piriformospora indica: A Novel Plant Growth Promoting Mycorrhizal Fungus","volume":"1","author":"Varma","year":"2012","journal-title":"Agric. Res."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1046\/j.1439-037X.2000.00394.x","article-title":"Root and Rhizosphere Colonization of Soybean (Glycine max (L.) Merr.) by Plant-Growth-Promoting Rhizobacteria at Low Root Zone Temperatures and under Short-Season Conditions","volume":"185","author":"Dashti","year":"2000","journal-title":"J. Agron. Crop Sci."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"3328","DOI":"10.1128\/AEM.68.7.3328-3338.2002","article-title":"Plant-dependent genotypic and phenotypic diversity of antagonistic rhizobacteria isolated from different Verticillium host plants","volume":"68","author":"Berg","year":"2002","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"1424","DOI":"10.1038\/ncomms2427","article-title":"The evolution and pathogenic mechanisms of the rice sheath blight pathogen","volume":"4","author":"Zheng","year":"2013","journal-title":"Nat. Commun."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"111655","DOI":"10.1016\/j.postharvbio.2021.111655","article-title":"Biocontrol of Rhizoctonia solani using volatile organic compounds of solanaceae seed-borne endophytic bacteria","volume":"181","author":"Marzouk","year":"2021","journal-title":"Postharvest Biol. Technol."},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Gkarmiri, K., Finlay, R.D., Alstr\u00f6m, S., Thomas, E., Cubeta, M.A., and H\u00f6gberg, N. (2015). Transcriptomic changes in the plant pathogenic fungus Rhizoctonia solani AG-3 in response to the antagonistic bacteria Serratia proteamaculans and Serratia plymuthica. BMC Genom., 16.","DOI":"10.1186\/s12864-015-1758-z"},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Li, P., Kwok, A.H.Y., Jiang, J., Ran, T., Xu, D., Wang, W., and Leung, F.C. (2015). Comparative Genome Analyses of Serratia marcescens FS14 Reveals Its High Antagonistic Potential. PLoS ONE, 10.","DOI":"10.1371\/journal.pone.0123061"},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Delgado, N., Olivera, M., C\u00e1diz, F., Bravo, G., Montenegro, I., Madrid, A., Fuentealba, C., Pedreschi, R., Salgado, E., and Besoain, X. (2021). Volatile Organic Compounds (VOCs) Produced by Gluconobacter cerinus and Hanseniaspora osmophila Displaying Control Effect against Table Grape-Rot Pathogens. Antibiotics, 10.","DOI":"10.3390\/antibiotics10060663"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1111\/1574-6968.12359","article-title":"VOC emission of various Serratia species and isolates and genome analysis of Serratia plymuthica 4Rx13","volume":"352","author":"Weise","year":"2014","journal-title":"FEMS Microbiol. Lett."},{"key":"ref_52","first-page":"e000178","article-title":"From plants to nematodes: Serratia grimesii BXF1 genome reveals an adaptation to the modulation of multi-species interactions","volume":"4","author":"Nascimento","year":"2018","journal-title":"Microb. Genom."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"993","DOI":"10.3389\/fmicb.2016.00993","article-title":"Impact of a Bacterial Volatile 2,3-Butanediol on Bacillus subtilis Rhizosphere Robustness","volume":"7","author":"Yi","year":"2016","journal-title":"Front. Microbiol."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"346","DOI":"10.1080\/07352680903241204","article-title":"Poplar and its Bacterial Endophytes: Coexistence and Harmony","volume":"28","author":"Taghavi","year":"2009","journal-title":"Crit. Rev. Plant Sci."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"748","DOI":"10.1128\/AEM.02239-08","article-title":"Genome survey and characterization of endophytic bacteria exhibiting a beneficial effect on growth and development of poplar trees","volume":"75","author":"Taghavi","year":"2009","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"Lakhan Singh, R., and Mondal, S. (2018). Microbial biotechnology and sustainable agriculture. Biotechnology for Sustainable Agriculture, Woodhead Publishing.","DOI":"10.1016\/B978-0-12-812160-3.00011-8"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"126297","DOI":"10.1016\/j.micres.2019.126297","article-title":"Engineering Bacillus velezensis with high production of acetoin primes strong induced systemic resistance in Arabidopsis thaliana","volume":"227","author":"Peng","year":"2019","journal-title":"Microbiol. Res."},{"key":"ref_58","doi-asserted-by":"crossref","unstructured":"Fu, L.H., Hu, K.D., Hu, L.Y., Li, Y.H., Hu, L.B., Yan, H., Liu, Y.S., and Zhang, H. (2014). An antifungal role of hydrogen sulfide on the postharvest pathogens Aspergillus niger and Penicillium italicum. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0104206"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"125704","DOI":"10.1155\/2014\/125704","article-title":"Inhibitory and toxic effects of volatiles emitted by strains of Pseudomonas and Serratia on growth and survival of selected microorganisms, Caenorhabditis elegans, and Drosophila melanogaster","volume":"2014","author":"Popova","year":"2014","journal-title":"Biomed. Res. Int."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"208","DOI":"10.5423\/PPJ.OA.07.2017.0158","article-title":"Isolation and Identification of Endophytic Bacteria with Plant Growth Promoting Activity and Biocontrol Potential from Wild Pistachio Trees","volume":"34","author":"Etminani","year":"2018","journal-title":"Plant Pathol. J."},{"key":"ref_61","first-page":"1335","article-title":"Plant growth promoting rhizobacteria Pseudomonas: A review","volume":"6","author":"Dorjey","year":"2017","journal-title":"Int. J. Curr. Microbiol."},{"key":"ref_62","doi-asserted-by":"crossref","unstructured":"Purushotham, P., Arun, P.V., Prakash, J.S., and Podile, A.R. (2012). Chitin binding proteins act synergistically with chitinases in Serratia proteamaculans 568. PLoS ONE, 7.","DOI":"10.1371\/journal.pone.0036714"},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"4951","DOI":"10.1128\/AEM.71.9.4951-4959.2005","article-title":"Use of plant growth promoting bacteria for biocontrol of plant diseases: Principles, mechanisms of action, and future prospects","volume":"71","author":"Compant","year":"2005","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1080\/09583150400016092","article-title":"Potential of Serratia marcescens strain B2 for biological control of rice sheath blight","volume":"15","author":"Someya","year":"2005","journal-title":"Biocontrol Sci. Technol."},{"key":"ref_65","doi-asserted-by":"crossref","unstructured":"Ben Slama, H., Triki, M.A., Bouket, A.C., Mefteh, B.F., Alenezi, F.N., Luptakova, L., Cherif-Silini, H., Vallat, A., Oszako, T., and Gharsallah, N. (2019). Screening of the High Rhizosphere Competent Limoniastrum monopetalum\u2019 Culturable Endophyte Microbiota Allows the Recovery of Multifaceted and Versatile Biocontrol Agents. Microorganisms, 7.","DOI":"10.3390\/microorganisms7080249"},{"key":"ref_66","unstructured":"Kumar, A., Vandana, R.S., Singh, M., and Pandey, K.D. (2019). Plant growth promoting rhizobacteria (PGPR). A promising approach to disease management. Microbes and Environmental Management, Studium Press."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"811","DOI":"10.1139\/w04-063","article-title":"Strategy to select and assess antagonistic bacteria for biological control of Rhizoctonia solani K\u00fchn","volume":"50","author":"Faltin","year":"2004","journal-title":"Can. J. Microbiol."},{"key":"ref_68","doi-asserted-by":"crossref","unstructured":"Kannan, V.R., and Bastas, K.K. (2015). Biocontrol mechanisms of siderophores against bacterial plant pathogens. Sustainable Approaches to Controlling Plant Pathogenic Bacteria, CRC Press. [1st ed.].","DOI":"10.1201\/b18892"},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"196","DOI":"10.1111\/1751-7915.12117","article-title":"Siderophores in environmental research: Roles and applications","volume":"7","author":"Ahmed","year":"2014","journal-title":"Microbial. Biotechnol."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"1964","DOI":"10.3389\/fmicb.2017.01964","article-title":"Magnitude and Mechanism of Siderophore-Mediated Competition at Low Iron Solubility in the Pseudomonas aeruginosa Pyochelin System","volume":"8","author":"Schiessl","year":"2017","journal-title":"Front. Microbiol."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1007\/BF02979698","article-title":"Induction of systemic resistance to Colletotrichum lindemuthianum in bean by a benzothiadiazole derivative and rhizobacteria","volume":"30","author":"Bigirimana","year":"2002","journal-title":"Phytoparasitica"},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1078\/0944-5013-00197","article-title":"Growth promoting influence of siderophore producing Pseudomonas strains GRP3A and PRS9 in maize (Zea mays L.) under iron limiting conditions","volume":"158","author":"Sharma","year":"2003","journal-title":"Microbiol. Res."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"593","DOI":"10.1094\/PHYTO.2001.91.6.593","article-title":"Role of iron in rhizobacteria mediated induced systemic resistance of cucumber","volume":"91","author":"Press","year":"2001","journal-title":"Phytopathology"},{"key":"ref_74","doi-asserted-by":"crossref","unstructured":"Singh, D.P., Gupta, V.K., and Prabha, R. (2019). Microbial Interventions in Agriculture and Environment: Rhizosphere, Microbiome and Agro-Ecology, Springer.","DOI":"10.1007\/978-981-13-8383-0"},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1002\/jobm.201100552","article-title":"Microbial siderophores: A mini review","volume":"53","author":"Saha","year":"2013","journal-title":"J. Basic Microbiol."},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"1606","DOI":"10.3389\/fmicb.2018.01606","article-title":"Soil Microbial Resources for Improving Fertilizers Efficiency in an Integrated Plant Nutrient Management System","volume":"9","author":"Bargaz","year":"2018","journal-title":"Front. Microbiol."},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"587","DOI":"10.1186\/2193-1801-2-587","article-title":"Phosphate solubilizing microbes: Sustainable approach for managing phosphorus deficiency in agricultural soils","volume":"2","author":"Sharma","year":"2013","journal-title":"Springerplus"},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1007\/s13199-015-0337-z","article-title":"Beneficial effects of native phosphate solubilizing bacteria on peanut (Arachis hypogaea L.) growth and phosphorus acquisition","volume":"66","author":"Anzuay","year":"2015","journal-title":"Symbiosis"},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"130","DOI":"10.1016\/j.aasci.2016.10.001","article-title":"Isolation and characterization of phosphate solubilizing bacterium Pseudomonas aeruginosa KUPSB12 with antibacterial potential from river Ganga, India","volume":"15","author":"Dipak","year":"2017","journal-title":"Ann. Agrar. Sci."},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"844","DOI":"10.4014\/jmb.1611.11057","article-title":"Phosphate solubilization and gene expression of phosphate-solubilizing bacterium Burkholderia multivorans WS-FJ9 under different levels of soluble phosphate","volume":"27","author":"Zeng","year":"2017","journal-title":"J. Microbiol. Biotechnol."},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"379","DOI":"10.1016\/j.jgeb.2017.06.005","article-title":"Phenotypic and genotypic characterization of phosphate solubilizing bacteria and their efficiency on the growth of maize","volume":"15","author":"Pande","year":"2017","journal-title":"J. Genet. Eng. Biotechnol."},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"288","DOI":"10.1016\/S1049-9644(02)00108-1","article-title":"The role of salicylic acid in induced systemic resistance elicited by plant growth-promoting rhizobacteria against blue mold of tobacco","volume":"25","author":"Zhang","year":"2002","journal-title":"Biol. Control"},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1007\/s10658-007-9157-1","article-title":"Dialogues of root-colonizing biocontrol pseudomonads","volume":"119","author":"Dubuis","year":"2007","journal-title":"Eur. J. Plant Pathol."},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1007\/s11104-008-9568-6","article-title":"The rhizosphere: A playground and battlefeld for soilborne pathogens and benefcial microorganisms","volume":"321","author":"Raaijmakers","year":"2009","journal-title":"Plant Soil"},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"3593","DOI":"10.1128\/JB.182.12.3593-3596.2000","article-title":"Exopolysaccharide production is required for development of Escherichia coli K-12 biofilm architecture","volume":"182","author":"Danese","year":"2000","journal-title":"J. Bacteriol."},{"key":"ref_86","doi-asserted-by":"crossref","unstructured":"Zhu, M.-L., Wu, X.-Q., Wang, Y.-H., and Dai, Y. (2020). Role of Biofilm Formation by Bacillus pumilus HR10 in Biocontrol against Pine Seedling Damping-Off Disease Caused by Rhizoctonia solani. Forests, 11.","DOI":"10.3390\/f11060652"},{"key":"ref_87","doi-asserted-by":"crossref","first-page":"1548","DOI":"10.3389\/fmicb.2016.01548","article-title":"Genes Required for the Anti-fungal Activity of a Bacterial Endophyte Isolated from a Corn Landrace Grown Continuously by Subsistence Farmers Since 1000 BC","volume":"7","author":"Shehata","year":"2016","journal-title":"Front. Microbiol."},{"key":"ref_88","doi-asserted-by":"crossref","unstructured":"Kandel, S.L., Joubert, P.M., and Doty, S.L. (2017). Bacterial Endophyte Colonization and Distribution within Plants. Microorganisms, 5.","DOI":"10.3390\/microorganisms5040077"},{"key":"ref_89","doi-asserted-by":"crossref","first-page":"2585","DOI":"10.1128\/AEM.04070-15","article-title":"Mechanisms of Bacterial (Serratia marcescens) Attachment to, Migration along, and Killing of Fungal Hyphae","volume":"82","author":"Hover","year":"2016","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_90","doi-asserted-by":"crossref","unstructured":"Chlebek, D., Pinski, A., \u017bur, J., Michalska, J., and Hupert-Kocurek, K. (2020). Genome Mining and Evaluation of the Biocontrol Potential of Pseudomonas fluorescens BRZ63, a New Endophyte of Oilseed Rape (Brassica napus L.) against Fungal Pathogens. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21228740"},{"key":"ref_91","doi-asserted-by":"crossref","first-page":"D309","DOI":"10.1093\/nar\/gky1085","article-title":"EggNOG 5.0: A hierarchical, functionally and phylogenetically annotated orthology resource based on 5090 organisms and 2502 viruses","volume":"47","author":"Szklarczyk","year":"2019","journal-title":"Nucleic Acids Res."},{"key":"ref_92","doi-asserted-by":"crossref","first-page":"D457","DOI":"10.1093\/nar\/gkv1070","article-title":"KEGG as a reference resource for gene and protein annotation","volume":"44","author":"Kanehisa","year":"2016","journal-title":"Nucleic Acids Res."},{"key":"ref_93","doi-asserted-by":"crossref","first-page":"W81","DOI":"10.1093\/nar\/gkz310","article-title":"AntiSMASH 5.0: Updates to the secondary metabolite genome mining pipeline","volume":"47","author":"Blin","year":"2019","journal-title":"Nucleic Acids Res."},{"key":"ref_94","doi-asserted-by":"crossref","first-page":"W445","DOI":"10.1093\/nar\/gks479","article-title":"dbCAN: A web resource for automated carbohydrate-active enzyme annotation","volume":"40","author":"Yin","year":"2012","journal-title":"Nucleic Acids Res."},{"key":"ref_95","doi-asserted-by":"crossref","first-page":"W95","DOI":"10.1093\/nar\/gky418","article-title":"dbCAN2: A meta server for automated carbohydrate-active enzyme annotation","volume":"46","author":"Zhang","year":"2018","journal-title":"Nucleic Acids Res."},{"key":"ref_96","doi-asserted-by":"crossref","first-page":"W30","DOI":"10.1093\/nar\/gkx343","article-title":"IslandViewer 4: Expanded prediction of genomic islands for larger-scale datasets","volume":"45","author":"Bertelli","year":"2017","journal-title":"Nucleic Acids Res."},{"key":"ref_97","doi-asserted-by":"crossref","first-page":"W88","DOI":"10.1093\/nar\/gkz423","article-title":"M1CR0B1AL1Z3R\u2014A user-friendly web server for the analysis of large-scale microbial genomics data","volume":"47","author":"Avram","year":"2019","journal-title":"Nucleic Acids Res."},{"key":"ref_98","doi-asserted-by":"crossref","first-page":"564","DOI":"10.1080\/10635150701472164","article-title":"Improvement of phylogenies after removing divergent and ambiguously aligned blocks from protein sequence alignments","volume":"56","author":"Talavera","year":"2007","journal-title":"Syst. Biol."},{"key":"ref_99","doi-asserted-by":"crossref","first-page":"1547","DOI":"10.1093\/molbev\/msy096","article-title":"MEGA X: Molecular evolutionary genetics analysis across computing platforms","volume":"35","author":"Kumar","year":"2018","journal-title":"Mol. Biol. Evol."},{"key":"ref_100","doi-asserted-by":"crossref","unstructured":"Vicente, C.S.L., Nascimento, F.X., Ikuyo, Y., Cock, P.J., Mota, M., and Hasegawa, K. (2016). The genome and genetics of a high oxidative stress tolerant Serratia sp. LCN16 isolated from the plant parasitic nematode Bursaphelenchus xylophilus. BMC Genom., 17.","DOI":"10.1186\/s12864-016-2626-1"},{"key":"ref_101","doi-asserted-by":"crossref","unstructured":"Zur, J., Pinski, A., Wojcieszynska, D., Smu\u0142ek, W., and Guzik, U. (2020). Diclofenac degradation\u2014Enzymes, genetic background and cellular alterations triggered in diclofenac-metabolizing strain Pseudomonas moorei KB4. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21186786"},{"key":"ref_102","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1016\/j.ijbiomac.2021.10.063","article-title":"Potential of Serratia plymuthica IV-11-34 strain for biodegradation of polylactide and poly(ethylene terephthalate)","volume":"193","author":"Richert","year":"2021","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_103","doi-asserted-by":"crossref","first-page":"S1","DOI":"10.1016\/j.ymeth.2010.01.005","article-title":"A practical approach to RT-qPCR-Publishing data that conform to the MIQE guidelines","volume":"50","author":"Taylor","year":"2010","journal-title":"Methods"},{"key":"ref_104","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real-time quantitative PCR and the 2\u2212\u2206\u2206CT method","volume":"25","author":"Livak","year":"2001","journal-title":"Methods"},{"key":"ref_105","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1351\/pac198759020257","article-title":"Measurement of cellulase activities","volume":"59","author":"Ghose","year":"1987","journal-title":"Pure Appl. Chem."},{"key":"ref_106","doi-asserted-by":"crossref","unstructured":"Rais, A., Jabeen, Z., Shair, F., Hafeez, F.Y., and Hassan, M.N. (2017). Bacillus spp., a bio-control agent enhances the activity of antioxidant defense enzymes in rice against Pyricularia oryzae. PLoS ONE, 12.","DOI":"10.1371\/journal.pone.0187412"},{"key":"ref_107","doi-asserted-by":"crossref","first-page":"1017","DOI":"10.1590\/S1517-83822011000300022","article-title":"Characterization of a chitinase with antifungal activity from a native Serratia marcescens B4A","volume":"42","author":"Zarei","year":"2011","journal-title":"Braz. J. Microbiol."},{"key":"ref_108","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1016\/j.ab.2016.03.014","article-title":"A new formula to calculate activity of superoxide dismutase in indirect assays","volume":"503","author":"Zhang","year":"2016","journal-title":"Anal. Biochem."},{"key":"ref_109","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1007\/s11270-015-2359-9","article-title":"Bioremediation of heavy metals by a novel bacterial strain Enterobacter cloaca and its antioxidant enzyme activity, flocculant production and protein expression in presence of lead, cadmium and nickel","volume":"226","author":"Banerjee","year":"2015","journal-title":"Water Air Soil Pollut."},{"key":"ref_110","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/0003-2697(76)90527-3","article-title":"A rapid and sensitive method for the quantitation of microgram quantities of proteins utilizing the principle of protein-dye binding","volume":"72","author":"Bradford","year":"1976","journal-title":"Anal. Biochem."},{"key":"ref_111","doi-asserted-by":"crossref","unstructured":"Johnston-Monje, D., and Raizada, M.N. (2013). Conservation and diversity of seed associated endophytes in Zea across boundaries of evolution, ethnography and ecology. PLoS ONE, 6.","DOI":"10.1371\/journal.pone.0020396"},{"key":"ref_112","first-page":"120","article-title":"Functional characterization of various plant growth promoting activity of Pseudomonas fluorescens and Bacillus subtilis from Aloe vera rhizosphere","volume":"6","author":"Syamala","year":"2017","journal-title":"J. Pharmacogn. Phytochem."},{"key":"ref_113","doi-asserted-by":"crossref","first-page":"22","DOI":"10.3103\/S1068367417010165","article-title":"Endophytes from maize with plant growth promotion and biocontrol activity under drought stress","volume":"43","author":"Sandhya","year":"2017","journal-title":"Russ. Agric. Sci."},{"key":"ref_114","unstructured":"Cappuccino, J.G., and Sherman, N. (1992). Biochemical Activities of Microorganisms. Microbiology, A Laboratory Manual, The Benjamin\/Cummings Publishing Co."},{"key":"ref_115","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1016\/0003-2697(87)90612-9","article-title":"Universal chemical assay for the detection and determination of siderophores","volume":"160","author":"Schwyn","year":"1987","journal-title":"Anal. Biochem."},{"key":"ref_116","first-page":"362","article-title":"Mobilization of phosphorus in soil in connection with vital activity of some microbial species","volume":"17","author":"Pikovskaya","year":"1948","journal-title":"Microbiology"},{"key":"ref_117","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1007\/s00374-013-0854-y","article-title":"The endophyte Enterobacter sp. FD17: A maize growth enhancer selected based on rigorous testing of plant beneficial traits and colonization characteristics","volume":"50","author":"Naveed","year":"2014","journal-title":"Biol. Fertil. Soils"},{"key":"ref_118","doi-asserted-by":"crossref","first-page":"872","DOI":"10.1136\/jcp.42.8.872","article-title":"New method for detecting slime production by coagulase negative staphylococci","volume":"42","author":"Freeman","year":"1989","journal-title":"J. Clin. Pathol."},{"key":"ref_119","first-page":"56","article-title":"Biochemical detection of N-Acyl homoserine lactone from biofilm-forming uropathogenic Escherichia coli isolated from urinary tract infection samples","volume":"3","author":"Taghadosi","year":"2015","journal-title":"Rep. Biochem. Mol. Biol."},{"key":"ref_120","doi-asserted-by":"crossref","unstructured":"Michalska, J., Pi\u0144ski, A., \u017bur, J., and Mrozik, A. (2020). Selecting Bacteria Candidates for the Bioaugmentation of Activated Sludge to Improve the Aerobic Treatment of Landfill Leachate. Water, 12.","DOI":"10.3390\/w12010140"}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/23\/24\/15561\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T01:36:34Z","timestamp":1760146594000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/23\/24\/15561"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,12,8]]},"references-count":120,"journal-issue":{"issue":"24","published-online":{"date-parts":[[2022,12]]}},"alternative-id":["ijms232415561"],"URL":"https:\/\/doi.org\/10.3390\/ijms232415561","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,12,8]]}}}