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To enlighten the role of PBM in the evolution of epithelial maturity in OM ulcers, the present study evaluated the effect of PBM with red (\u03bb) wavelength of 660 nanometers (nm) and infrared of 780 nm in radio-induced OM wounds on the tongue of rats, eight and twenty days after irradiation with single dose of 20 Gy. The percentage area corresponding to positive staining for cytokeratin 10 (CK10) and 14 (CK14) proteins was evaluated in the epithelial area of the lesions, using an immunohistochemical technique (IHC), 8 and 20 days after the induction of lesions, and compared with an untreated control group. CK10 was significantly more expressed in the group treated with 660 nm PBM. CK14 did not show quantitative differences between the groups evaluated. However, whereas in the groups treated with PBM, CK14 was already restricted to the basal layer of the epithelium, as expected in healthy epithelia, in control group it was also expressed in upper layers of the epithelium. In this work, PBM was able to improve epithelial maturity of the repaired OM wound, especially in the 660 nm group.<\/jats:p>","DOI":"10.3390\/ijms232415611","type":"journal-article","created":{"date-parts":[[2022,12,9]],"date-time":"2022-12-09T06:14:00Z","timestamp":1670566440000},"page":"15611","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Does Photobiomodulation Affects CK10 and CK14 in Oral Mucositis Radioinduced Repair?"],"prefix":"10.3390","volume":"23","author":[{"given":"Ariane Venzon Naia","family":"Sardo","sequence":"first","affiliation":[{"name":"Center for Lasers and Applications, Instituto de Pesquisas Energeticas e Nucelares IPEN-CNEN, S\u00e3o Paulo CEP 05508-000, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ma\u00edra Franco","family":"Andrade","sequence":"additional","affiliation":[{"name":"Center for Lasers and Applications, Instituto de Pesquisas Energeticas e Nucelares IPEN-CNEN, S\u00e3o Paulo CEP 05508-000, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anaeliza","family":"Figueiredo","sequence":"additional","affiliation":[{"name":"School of Dentistry, University of S\u00e3o Paulo, S\u00e3o Paulo CEP 05508-000, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fl\u00e1via Cristina Perillo","family":"Rosin","sequence":"additional","affiliation":[{"name":"School of Dentistry, University of S\u00e3o Paulo, S\u00e3o Paulo CEP 05508-000, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5774-0750","authenticated-orcid":false,"given":"Luciana","family":"Corr\u00eaa","sequence":"additional","affiliation":[{"name":"School of Dentistry, University of S\u00e3o Paulo, S\u00e3o Paulo CEP 05508-000, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7404-9606","authenticated-orcid":false,"given":"Denise Maria","family":"Zezell","sequence":"additional","affiliation":[{"name":"Center for Lasers and Applications, Instituto de Pesquisas Energeticas e Nucelares IPEN-CNEN, S\u00e3o Paulo CEP 05508-000, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,12,9]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1995","DOI":"10.1002\/cncr.20162","article-title":"Perspectives on Cancer Therapy-Induced Mucosal Injury: Pathogenesis, Measurement, Epidemiology, and Consequences for Patients","volume":"100","author":"Sonis","year":"2004","journal-title":"Cancer"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1016\/j.oraloncology.2015.10.022","article-title":"Cost-Effectiveness of Low-Level Laser Therapy (LLLT) in Head and Neck Cancer Patients Receiving Concurrent Chemoradiation","volume":"52","author":"Antunes","year":"2016","journal-title":"Oral Oncol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"400","DOI":"10.3322\/caac.21157","article-title":"Oral Complications of Cancer and Cancer Therapy","volume":"62","author":"Epstein","year":"2012","journal-title":"CA Cancer J. Clin."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"395","DOI":"10.1016\/j.oraloncology.2006.04.011","article-title":"Nuclear Factor-\u039aB (NF-\u039aB) and Cyclooxygenase-2 (COX-2) Expression in the Oral Mucosa Following Cancer Chemotherapy","volume":"43","author":"Logan","year":"2007","journal-title":"Oral Oncol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"597","DOI":"10.1111\/j.1601-0825.2010.01681.x","article-title":"New Thoughts on the Initiation of Mucositis","volume":"16","author":"Sonis","year":"2010","journal-title":"Oral Dis."},{"key":"ref_6","first-page":"e201","article-title":"Treatment of Oral Mucositis Due to Chemotherapy","volume":"8","year":"2016","journal-title":"J. Clin. Exp. Dent."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"3949","DOI":"10.1007\/s00520-019-04848-4","article-title":"Systematic Review of Basic Oral Care for the Management of Oral Mucositis in Cancer Patients and Clinical Practice Guidelines","volume":"27","author":"Hong","year":"2019","journal-title":"Support. Care Cancer"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"4423","DOI":"10.1002\/cncr.33100","article-title":"MASCC\/ISOO Clinical Practice Guidelines for the Management of Mucositis Secondary to Cancer Therapy","volume":"126","author":"Elad","year":"2020","journal-title":"Cancer"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"316","DOI":"10.1111\/petr.12440","article-title":"Oral Mucositis in Pediatric Patients Undergoing Hematopoietic Stem Cell Transplantation: Clinical Outcomes in a Context of Specialized Oral Care Using Low-Level Laser Therapy","volume":"19","author":"Eduardo","year":"2015","journal-title":"Pediatr. Transplant."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1016\/j.oraloncology.2017.11.001","article-title":"A Network Meta-Analysis in Comparing Prophylactic Treatments of Radiotherapy-Induced Oral Mucositis for Patients with Head and Neck Cancers Receiving Radiotherapy","volume":"75","author":"Peng","year":"2017","journal-title":"Oral Oncol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1813","DOI":"10.1007\/s10103-018-2549-y","article-title":"Treatment of Mucositis with Combined 660- and 808-Nm-Wavelength Low-Level Laser Therapy Reduced Mucositis Grade, Pain, and Use of Analgesics: A Parallel, Single-Blind, Two-Arm Controlled Study","volume":"33","author":"Soares","year":"2018","journal-title":"Lasers Med. Sci."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"3319","DOI":"10.1007\/s00520-018-4262-6","article-title":"Photobiomodulation Therapy in the Management of Oral Mucositis: Search for the Optimal Clinical Treatment Parameters","volume":"26","author":"Elad","year":"2018","journal-title":"Support. Care Cancer"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"226","DOI":"10.1016\/j.canrad.2015.11.006","article-title":"Actualit\u00e9s de la Pr\u00e9vention et du Traitement des Mucites Orales Chez les Enfants Canc\u00e9reux: Recommandations Pratiques","volume":"20","author":"Eljahd","year":"2016","journal-title":"Cancer\/Radiotherapie"},{"key":"ref_14","first-page":"17","article-title":"Oral Mucositis Due to High-Dose Chemotherapy and\/or Head and Neck Radiation Therapy","volume":"2019","author":"Lalla","year":"2019","journal-title":"J. Natl. Cancer Inst. Monogr."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"3969","DOI":"10.1007\/s00520-019-04890-2","article-title":"Systematic Review of Photobiomodulation for the Management of Oral Mucositis in Cancer Patients and Clinical Practice Guidelines","volume":"27","author":"Zadik","year":"2019","journal-title":"Support. Care Cancer"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1097\/SPC.0000000000000421","article-title":"Animal Models of Mucositis: Critical Tools for Advancing Pathobiological Understanding and Identifying Therapeutic Targets","volume":"13","author":"Wardill","year":"2019","journal-title":"Curr. Opin. Support. Palliat. Care"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/0092-8674(82)90400-7","article-title":"The Catalog of Human Cytokeratins: Patterns of Expression in Normal Epithelia, Tumors and Cultured Cells","volume":"31","author":"Moll","year":"1982","journal-title":"Cell"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1016\/S0955-0674(01)00301-5","article-title":"\u201cHard\u201d and \u201cSoft\u201d Principles Defining the Structure, Function and Regulation of Keratin Intermediate Filaments","volume":"14","author":"Coulombe","year":"2002","journal-title":"Curr. Opin. Cell Biol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1257","DOI":"10.1083\/jcb.101.4.1257","article-title":"Expression of Epidermal Keratins and Filaggrin during Human Fetal Skin Development","volume":"101","author":"Dale","year":"1985","journal-title":"J. Cell Biol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"998","DOI":"10.1038\/sj.jid.5700786","article-title":"Keratinocyte-Fibroblast Interactions in Wound Healing","volume":"127","author":"Werner","year":"2007","journal-title":"J. Investig. Dermatol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1016\/j.tripleo.2005.03.033","article-title":"Cytokeratin Expression in Initial Oral Mucositis of Head and Neck Irradiated Patients","volume":"101","author":"Bonan","year":"2006","journal-title":"Oral Surg. Oral Med. Oral Pathol. Oral Radiol. Endodontology"},{"key":"ref_22","first-page":"187","article-title":"Cytokeratin Profiles in Oral Epithelial: A Review and a New Classification","volume":"19","author":"Sawaf","year":"1991","journal-title":"J. Biol. Buccale"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1046\/j.0022-202x.2001.01651.x","article-title":"Human \u03b2-Defensin-2 Production in Keratinocytes Is Regulated by Interleukin-1, Bacteria, and the State of Differentiation","volume":"118","author":"Liu","year":"2002","journal-title":"J. Investig. Dermatol."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Piipponen, M., Li, D., and Land\u00e9n, N.X. (2020). The Immune Functions of Keratinocytes in Skin Wound Healing. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21228790"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1177\/1534734617744951","article-title":"ImageJ: A Free, Easy, and Reliable Method to Measure Leg Ulcers Using Digital Pictures","volume":"16","year":"2017","journal-title":"Int. J. Low. Extrem. Wounds"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1485","DOI":"10.1093\/bioinformatics\/btaa847","article-title":"Colour Deconvolution: Stain Unmixing in Histological Imaging","volume":"37","author":"Landini","year":"2021","journal-title":"Bioinformatics"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"154","DOI":"10.3389\/fonc.2016.00154","article-title":"Single-Dose Radiation-Induced Oral Mucositis Mouse Model","volume":"6","author":"Maria","year":"2016","journal-title":"Front. Oncol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"72739","DOI":"10.18632\/oncotarget.20286","article-title":"Alpha Lipoic Acid Attenuates Radiation-Induced Oral Mucositis in Rats","volume":"8","author":"Kim","year":"2017","journal-title":"Oncotarget"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1007\/s00066-019-01532-8","article-title":"Modulation of Radiation-Induced Oral Mucositis (Mouse) by Dermatan Sulfate: Effects on Differentiation Processes","volume":"196","author":"Cini","year":"2020","journal-title":"Strahlenther. Onkol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1015","DOI":"10.1016\/j.oraloncology.2009.08.006","article-title":"Mucositis: The Impact, Biology and Therapeutic Opportunities of Oral Mucositis","volume":"45","author":"Sonis","year":"2009","journal-title":"Oral Oncol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"111850","DOI":"10.1016\/j.jphotobiol.2020.111850","article-title":"Improvement of Full-Thickness Rat Skin Wounds by Photobiomodulation Therapy (PBMT): A Dosimetric Study","volume":"206","author":"Chen","year":"2020","journal-title":"J. Photochem. Photobiol. B Biol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"795","DOI":"10.1002\/jbio.201400064","article-title":"Low-Level Laser Irradiation Promotes the Proliferation and Maturation of Keratinocytes during Epithelial Wound Repair","volume":"8","author":"Sperandio","year":"2015","journal-title":"J. Biophotonics"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1007\/s10103-017-2313-8","article-title":"CDNA Microarray Analysis of Human Keratinocytes Cells of Patients Submitted to Chemoradiotherapy and Oral Photobiomodulation Therapy: Pilot Study","volume":"33","author":"Antunes","year":"2018","journal-title":"Lasers Med. Sci."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"2543","DOI":"10.1088\/0022-3727\/38\/15\/004","article-title":"Optical Properties of Human Skin, Subcutaneous and Mucous Tissues in the Wavelength Range from 400 to 2000 Nm","volume":"38","author":"Bashkatov","year":"2005","journal-title":"J. Phys. D Appl. Phys."},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Rusu, D., Calenic, B., Greabu, M., Kralev, A., Boariu, M., Bojin, F., Anghel, S., Paunescu, V., Vela, O., and Calniceanu, H. (2016). Evaluation of Oral Keratinocyte Progenitor and T-Lymphocite Cells Response during Early Healing after Augmentation of Keratinized Gingiva with a 3D Collagen Matrix\u2014A Pilot Study. BMC Oral Health, 17.","DOI":"10.1186\/s12903-016-0240-x"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"2557","DOI":"10.1007\/s00520-015-3048-3","article-title":"Topical Application of Aloe Vera and Vitamin E on Induced Ulcers on the Tongue of Rats Subjected to Radiation: Clinical and Histological Evaluation","volume":"24","author":"Cherubini","year":"2016","journal-title":"Support. Care Cancer"},{"key":"ref_37","first-page":"481","article-title":"Comparison of Ketamine-Xylazine and Ketamine-Dexmedetomidine Anesthesia and Intraperitoneal Tolerance in Rats","volume":"52","author":"Wellington","year":"2013","journal-title":"J. Am. Assoc. Lab. Anim. Sci."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/23\/24\/15611\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T01:37:11Z","timestamp":1760146631000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/23\/24\/15611"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,12,9]]},"references-count":37,"journal-issue":{"issue":"24","published-online":{"date-parts":[[2022,12]]}},"alternative-id":["ijms232415611"],"URL":"https:\/\/doi.org\/10.3390\/ijms232415611","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,12,9]]}}}